Proteome-pI 2.0 - Proteome Isoelectric Point Database

Spizellomyces punctatus (strain DAOM BR117)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Spizellomycetales; Spizellomycetaceae; Spizellomyces; Spizellomyces punctatus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 9267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0L0HU77|A0A0L0HU77_SPIPD Uncharacterized protein OS=Spizellomyces punctatus (strain DAOM BR117) OX=645134 GN=SPPG_00189 PE=4 SV=1

MM1 pKa = 7.32GNAARR6 pKa = 11.84TFAIPPGTYY15 pKa = 10.32SPDD18 pKa = 3.76DD19 pKa = 3.79LVDD22 pKa = 3.59ALTAGFNSTGTQAYY36 pKa = 6.47TVEE39 pKa = 4.21YY40 pKa = 10.66NHH42 pKa = 7.03INSQITVTAGTTPFKK57 pKa = 10.88VLYY60 pKa = 8.99PQTTASKK67 pKa = 10.9LLGLQQTQQSDD78 pKa = 3.68PGTSITFSNPIDD90 pKa = 3.63LTGVKK95 pKa = 8.66MVLVASSNIRR105 pKa = 11.84SNDD108 pKa = 3.2VIYY111 pKa = 10.94AGTDD115 pKa = 3.27DD116 pKa = 4.91LNIIASIPVSQEE128 pKa = 3.18IEE130 pKa = 4.29TVLCAQNYY138 pKa = 9.2FDD140 pKa = 4.88TDD142 pKa = 4.36FIDD145 pKa = 3.92TEE147 pKa = 4.39SGLVSTIDD155 pKa = 3.1IHH157 pKa = 8.72LLDD160 pKa = 5.36AEE162 pKa = 4.55TLLPLDD168 pKa = 3.97LQGKK172 pKa = 9.23GFTLVLDD179 pKa = 3.95CHH181 pKa = 7.17SSGFADD187 pKa = 4.47DD188 pKa = 3.91

Molecular weight: 20.04 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0L0HQ18|A0A0L0HQ18_SPIPD Uncharacterized protein OS=Spizellomyces punctatus (strain DAOM BR117) OX=645134 GN=SPPG_08985 PE=4 SV=1

MM1 pKa = 7.24VEE3 pKa = 3.97KK4 pKa = 10.61RR5 pKa = 11.84PARR8 pKa = 11.84GGRR11 pKa = 11.84GGGMRR16 pKa = 11.84GGARR20 pKa = 11.84GGRR23 pKa = 11.84GGFGGGRR30 pKa = 11.84GSFGGGRR37 pKa = 11.84GSFGGGRR44 pKa = 11.84GAPRR48 pKa = 11.84GASRR52 pKa = 11.84GGRR55 pKa = 11.84GRR57 pKa = 11.84GRR59 pKa = 11.84GFF61 pKa = 3.26

Molecular weight: 5.81 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
8940	327	9267	4819746	51	7024	520.1	57.77

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.209 ± 0.023	1.266 ± 0.011	5.676 ± 0.016	6.508 ± 0.032	3.535 ± 0.016	6.389 ± 0.022	2.428 ± 0.012	4.941 ± 0.015	5.271 ± 0.025	9.157 ± 0.026

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.224 ± 0.009	3.612 ± 0.014	5.82 ± 0.026	4.037 ± 0.017	6.294 ± 0.022	7.909 ± 0.027	6.145 ± 0.024	6.648 ± 0.017	1.248 ± 0.008	2.683 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski