Klebsiella phage KMI9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Slopekvirus; unclassified Slopekvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NDF9|A0A5B9NDF9_9CAUD Uncharacterized protein OS=Klebsiella phage KMI9 OX=2601611 GN=KMI9_151 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 4.6IVDD5 pKa = 3.85YY6 pKa = 11.59VNILNEE12 pKa = 3.76EE13 pKa = 3.94GRR15 pKa = 11.84YY16 pKa = 9.5KK17 pKa = 10.75DD18 pKa = 3.61LYY20 pKa = 11.41KK21 pKa = 10.37MFGFDD26 pKa = 4.11VIVAVQQDD34 pKa = 4.0SYY36 pKa = 11.92NEE38 pKa = 4.02YY39 pKa = 10.37VSLGFTLIKK48 pKa = 10.64DD49 pKa = 3.8NYY51 pKa = 10.02HH52 pKa = 5.13EE53 pKa = 4.45TKK55 pKa = 10.12VWTFTVLNSMDD66 pKa = 4.29EE67 pKa = 4.23DD68 pKa = 4.01SVIYY72 pKa = 10.73CFDD75 pKa = 3.41STITNMISDD84 pKa = 4.04ISQRR88 pKa = 11.84IIDD91 pKa = 3.75EE92 pKa = 4.34TQII95 pKa = 3.48

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NCJ4|A0A5B9NCJ4_9CAUD Uncharacterized protein OS=Klebsiella phage KMI9 OX=2601611 GN=KMI9_164 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.17RR3 pKa = 11.84SYY5 pKa = 11.65NMNEE9 pKa = 3.59LVFFKK14 pKa = 10.23TRR16 pKa = 11.84RR17 pKa = 11.84EE18 pKa = 3.6ARR20 pKa = 11.84AYY22 pKa = 10.51VLAAGKK28 pKa = 7.95NTSAAIDD35 pKa = 3.7MGTDD39 pKa = 3.15KK40 pKa = 11.32AVGARR45 pKa = 11.84WAAVIVIAAPVTPVKK60 pKa = 10.13TLVLGARR67 pKa = 11.84HH68 pKa = 6.69AEE70 pKa = 4.22TLNTAASGRR79 pKa = 11.84GHH81 pKa = 5.46EE82 pKa = 4.17VKK84 pKa = 10.57VFKK87 pKa = 10.58KK88 pKa = 10.43RR89 pKa = 11.84SYY91 pKa = 10.47LSCARR96 pKa = 11.84GG97 pKa = 3.1

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

252

0

252

53350

38

1327

211.7

24.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.643 ± 0.159

1.162 ± 0.072

6.611 ± 0.11

6.86 ± 0.165

4.092 ± 0.117

6.341 ± 0.214

1.762 ± 0.082

6.851 ± 0.134

7.423 ± 0.179

7.485 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.932 ± 0.092

5.492 ± 0.129

3.546 ± 0.085

3.286 ± 0.11

4.761 ± 0.115

6.071 ± 0.145

5.978 ± 0.219

6.924 ± 0.125

1.318 ± 0.053

4.463 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski