Priestia filamentosa

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4KI16|A0A0H4KI16_9BACI Peptidase OS=Priestia filamentosa OX=1402861 GN=BEH_10360 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.0TPEE5 pKa = 4.0QLIQEE10 pKa = 4.67DD11 pKa = 3.83KK12 pKa = 10.06VAFYY16 pKa = 11.12GSFISSMVYY25 pKa = 9.68INEE28 pKa = 4.13AVTEE32 pKa = 4.73AIEE35 pKa = 4.0EE36 pKa = 4.3MVAVGYY42 pKa = 10.65VSVEE46 pKa = 4.16SEE48 pKa = 4.32SMVQRR53 pKa = 11.84IEE55 pKa = 4.32AFADD59 pKa = 3.43LLGYY63 pKa = 9.29EE64 pKa = 4.59LEE66 pKa = 4.44VMPDD70 pKa = 3.67ADD72 pKa = 5.63SDD74 pKa = 4.33DD75 pKa = 3.39YY76 pKa = 11.16WYY78 pKa = 10.45DD79 pKa = 3.56VEE81 pKa = 4.5VVNWQVKK88 pKa = 7.05SS89 pKa = 3.54

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4KRC7|A0A0H4KRC7_9BACI 50S ribosomal protein L15 OS=Priestia filamentosa OX=1402861 GN=rplO PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 10.24VHH16 pKa = 5.71GFRR19 pKa = 11.84NRR21 pKa = 11.84MSSKK25 pKa = 10.15NGRR28 pKa = 11.84HH29 pKa = 4.53ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.09GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5042

0

5042

1427216

27

4076

283.1

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.671 ± 0.037

0.785 ± 0.01

4.715 ± 0.031

7.717 ± 0.049

4.638 ± 0.032

6.936 ± 0.047

2.175 ± 0.018

7.667 ± 0.032

7.288 ± 0.036

9.949 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.018

4.366 ± 0.024

3.482 ± 0.021

3.54 ± 0.023

4.002 ± 0.026

6.269 ± 0.028

5.503 ± 0.038

7.024 ± 0.029

0.999 ± 0.011

3.613 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski