Marchantia polymorpha subsp. ruderalis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Marchantiophyta; Marchantiopsida; Marchantiidae; Marchantiales; Marchantiaceae; Marchantia; Marchantia polymorpha

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A176VNU6|A0A176VNU6_MARPO Uncharacterized protein OS=Marchantia polymorpha subsp. ruderalis OX=1480154 GN=AXG93_242s1340 PE=4 SV=1
MM1 pKa = 7.39AQTEE5 pKa = 4.19LALPSGRR12 pKa = 11.84LQRR15 pKa = 11.84CALAVLLLFLNLPAYY30 pKa = 8.54PINEE34 pKa = 3.81ICKK37 pKa = 10.34AMDD40 pKa = 4.91SIPEE44 pKa = 4.1SDD46 pKa = 3.11SMARR50 pKa = 11.84IFAAMDD56 pKa = 3.17VFDD59 pKa = 5.6NYY61 pKa = 10.86TGDD64 pKa = 3.89LACFDD69 pKa = 4.92IYY71 pKa = 11.23DD72 pKa = 4.82DD73 pKa = 3.79PHH75 pKa = 8.72GMSGWDD81 pKa = 3.08

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A176VRM0|A0A176VRM0_MARPO Uncharacterized protein OS=Marchantia polymorpha subsp. ruderalis OX=1480154 GN=AXG93_285s1370 PE=4 SV=1
MM1 pKa = 7.37TMYY4 pKa = 10.85DD5 pKa = 3.88DD6 pKa = 4.69SDD8 pKa = 3.71PTRR11 pKa = 11.84GMLGHH16 pKa = 6.99GGMGMGASMRR26 pKa = 11.84HH27 pKa = 5.28TGQPSTPVFRR37 pKa = 11.84VVGPIPACGYY47 pKa = 10.35APEE50 pKa = 4.58FNTALSHH57 pKa = 6.1SPSPKK62 pKa = 8.29STLSRR67 pKa = 11.84LHH69 pKa = 6.87DD70 pKa = 4.02FARR73 pKa = 11.84RR74 pKa = 11.84NYY76 pKa = 7.87KK77 pKa = 8.63TRR79 pKa = 11.84SRR81 pKa = 11.84SRR83 pKa = 11.84LVSALLIVTEE93 pKa = 4.02QARR96 pKa = 11.84ACASAWACARR106 pKa = 11.84AQPQAPEE113 pKa = 4.08SQRR116 pKa = 11.84TRR118 pKa = 11.84MRR120 pKa = 11.84YY121 pKa = 9.33FLGKK125 pKa = 9.23PWGQRR130 pKa = 11.84KK131 pKa = 7.74YY132 pKa = 10.35VRR134 pKa = 11.84RR135 pKa = 11.84DD136 pKa = 2.82GNAEE140 pKa = 3.82HH141 pKa = 7.14GSGKK145 pKa = 10.22KK146 pKa = 9.84SPAWRR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84KK154 pKa = 8.57VTAAGEE160 pKa = 4.2SRR162 pKa = 11.84AGDD165 pKa = 3.59EE166 pKa = 4.34TVSAVTGEE174 pKa = 4.29SVRR177 pKa = 11.84NSGVTVPTTEE187 pKa = 4.59PLPLDD192 pKa = 3.65LTFLISPQGQNPWLIPSRR210 pKa = 11.84ALDD213 pKa = 3.49GNARR217 pKa = 11.84RR218 pKa = 11.84EE219 pKa = 4.31LRR221 pKa = 11.84CSPLPHH227 pKa = 6.79GSSEE231 pKa = 4.05PAVAEE236 pKa = 4.16LTCGFGVGPLKK247 pKa = 10.56FGHH250 pKa = 7.15DD251 pKa = 4.26NFPVILPRR259 pKa = 11.84VRR261 pKa = 3.77

Molecular weight: 28.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17951

0

17951

7780001

66

7651

433.4

47.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.302 ± 0.018

1.656 ± 0.008

5.344 ± 0.012

6.903 ± 0.022

3.552 ± 0.012

7.233 ± 0.019

2.226 ± 0.008

4.236 ± 0.012

5.335 ± 0.018

9.397 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.007

3.446 ± 0.01

5.198 ± 0.021

3.957 ± 0.014

6.457 ± 0.017

8.69 ± 0.022

5.14 ± 0.011

6.927 ± 0.014

1.334 ± 0.007

2.361 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski