Amino acid dipepetide frequency for Enterococcus phage phiSHEF5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.397AlaAla: 0.397 ± 0.175
0.238AlaCys: 0.238 ± 0.153
3.255AlaAsp: 3.255 ± 0.481
3.334AlaGlu: 3.334 ± 0.494
2.382AlaPhe: 2.382 ± 0.541
2.937AlaGly: 2.937 ± 0.541
1.111AlaHis: 1.111 ± 0.337
4.684AlaIle: 4.684 ± 0.908
6.192AlaLys: 6.192 ± 0.685
4.446AlaLeu: 4.446 ± 0.803
3.096AlaMet: 3.096 ± 0.501
3.811AlaAsn: 3.811 ± 0.688
2.064AlaPro: 2.064 ± 0.352
1.508AlaGln: 1.508 ± 0.347
1.747AlaArg: 1.747 ± 0.383
2.937AlaSer: 2.937 ± 0.442
4.366AlaThr: 4.366 ± 0.615
4.684AlaVal: 4.684 ± 0.626
0.715AlaTrp: 0.715 ± 0.215
2.461AlaTyr: 2.461 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
0.318CysAla: 0.318 ± 0.143
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.179
0.635CysGlu: 0.635 ± 0.231
0.159CysPhe: 0.159 ± 0.111
0.556CysGly: 0.556 ± 0.22
0.159CysHis: 0.159 ± 0.099
0.635CysIle: 0.635 ± 0.238
0.715CysLys: 0.715 ± 0.235
0.476CysLeu: 0.476 ± 0.187
0.159CysMet: 0.159 ± 0.124
0.556CysAsn: 0.556 ± 0.203
0.0CysPro: 0.0 ± 0.0
0.079CysGln: 0.079 ± 0.069
0.159CysArg: 0.159 ± 0.1
0.397CysSer: 0.397 ± 0.174
0.238CysThr: 0.238 ± 0.144
0.318CysVal: 0.318 ± 0.146
0.079CysTrp: 0.079 ± 0.074
0.159CysTyr: 0.159 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
3.176AspAla: 3.176 ± 0.578
0.238AspCys: 0.238 ± 0.128
2.699AspAsp: 2.699 ± 0.537
4.525AspGlu: 4.525 ± 0.637
2.937AspPhe: 2.937 ± 0.465
4.684AspGly: 4.684 ± 0.561
0.556AspHis: 0.556 ± 0.202
4.763AspIle: 4.763 ± 0.734
5.002AspLys: 5.002 ± 0.69
4.684AspLeu: 4.684 ± 0.558
2.382AspMet: 2.382 ± 0.409
4.922AspAsn: 4.922 ± 0.57
2.144AspPro: 2.144 ± 0.418
1.508AspGln: 1.508 ± 0.266
1.985AspArg: 1.985 ± 0.366
3.334AspSer: 3.334 ± 0.397
3.414AspThr: 3.414 ± 0.63
3.811AspVal: 3.811 ± 0.503
0.873AspTrp: 0.873 ± 0.243
3.334AspTyr: 3.334 ± 0.567
0.0AspXaa: 0.0 ± 0.0
Glu
5.002GluAla: 5.002 ± 0.747
0.397GluCys: 0.397 ± 0.22
3.811GluAsp: 3.811 ± 0.731
7.542GluGlu: 7.542 ± 1.538
3.414GluPhe: 3.414 ± 0.569
5.002GluGly: 5.002 ± 0.585
1.667GluHis: 1.667 ± 0.362
3.811GluIle: 3.811 ± 0.537
6.113GluLys: 6.113 ± 0.638
9.686GluLeu: 9.686 ± 1.164
2.62GluMet: 2.62 ± 0.535
3.652GluAsn: 3.652 ± 0.473
2.461GluPro: 2.461 ± 0.598
3.97GluGln: 3.97 ± 0.607
3.97GluArg: 3.97 ± 0.579
3.652GluSer: 3.652 ± 0.434
4.763GluThr: 4.763 ± 0.708
7.304GluVal: 7.304 ± 0.988
1.588GluTrp: 1.588 ± 0.333
3.573GluTyr: 3.573 ± 0.711
0.0GluXaa: 0.0 ± 0.0
Phe
1.667PheAla: 1.667 ± 0.295
0.318PheCys: 0.318 ± 0.162
2.779PheAsp: 2.779 ± 0.6
2.858PheGlu: 2.858 ± 0.531
1.27PhePhe: 1.27 ± 0.399
3.176PheGly: 3.176 ± 0.535
0.318PheHis: 0.318 ± 0.183
4.684PheIle: 4.684 ± 0.644
4.128PheLys: 4.128 ± 0.667
1.985PheLeu: 1.985 ± 0.353
1.27PheMet: 1.27 ± 0.328
3.334PheAsn: 3.334 ± 0.517
0.635PhePro: 0.635 ± 0.188
2.064PheGln: 2.064 ± 0.441
1.747PheArg: 1.747 ± 0.383
1.667PheSer: 1.667 ± 0.4
3.811PheThr: 3.811 ± 0.65
2.54PheVal: 2.54 ± 0.38
0.476PheTrp: 0.476 ± 0.19
1.191PheTyr: 1.191 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
3.89GlyAla: 3.89 ± 1.051
0.318GlyCys: 0.318 ± 0.131
2.937GlyAsp: 2.937 ± 0.44
3.731GlyGlu: 3.731 ± 0.594
3.414GlyPhe: 3.414 ± 0.484
4.208GlyGly: 4.208 ± 1.272
0.715GlyHis: 0.715 ± 0.274
5.478GlyIle: 5.478 ± 0.849
6.192GlyLys: 6.192 ± 0.754
6.272GlyLeu: 6.272 ± 1.066
1.588GlyMet: 1.588 ± 0.315
4.366GlyAsn: 4.366 ± 0.619
0.715GlyPro: 0.715 ± 0.282
1.905GlyGln: 1.905 ± 0.339
2.382GlyArg: 2.382 ± 0.324
3.334GlySer: 3.334 ± 0.585
4.287GlyThr: 4.287 ± 0.763
4.446GlyVal: 4.446 ± 0.683
0.873GlyTrp: 0.873 ± 0.244
2.302GlyTyr: 2.302 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
0.715HisAla: 0.715 ± 0.225
0.159HisCys: 0.159 ± 0.099
0.794HisAsp: 0.794 ± 0.239
1.032HisGlu: 1.032 ± 0.299
0.953HisPhe: 0.953 ± 0.316
0.953HisGly: 0.953 ± 0.272
0.238HisHis: 0.238 ± 0.129
0.953HisIle: 0.953 ± 0.234
1.429HisLys: 1.429 ± 0.356
1.191HisLeu: 1.191 ± 0.32
0.318HisMet: 0.318 ± 0.149
1.191HisAsn: 1.191 ± 0.3
0.397HisPro: 0.397 ± 0.153
0.635HisGln: 0.635 ± 0.194
0.794HisArg: 0.794 ± 0.223
0.476HisSer: 0.476 ± 0.208
1.032HisThr: 1.032 ± 0.34
1.032HisVal: 1.032 ± 0.32
0.0HisTrp: 0.0 ± 0.0
0.953HisTyr: 0.953 ± 0.322
0.0HisXaa: 0.0 ± 0.0
Ile
3.89IleAla: 3.89 ± 0.529
0.556IleCys: 0.556 ± 0.207
6.034IleAsp: 6.034 ± 0.651
6.907IleGlu: 6.907 ± 0.929
2.302IlePhe: 2.302 ± 0.517
3.97IleGly: 3.97 ± 0.496
1.032IleHis: 1.032 ± 0.263
4.208IleIle: 4.208 ± 0.5
6.431IleLys: 6.431 ± 0.667
4.922IleLeu: 4.922 ± 0.699
1.667IleMet: 1.667 ± 0.397
4.922IleAsn: 4.922 ± 0.644
2.54IlePro: 2.54 ± 0.35
2.937IleGln: 2.937 ± 0.418
1.667IleArg: 1.667 ± 0.321
4.287IleSer: 4.287 ± 0.509
3.652IleThr: 3.652 ± 0.446
3.811IleVal: 3.811 ± 0.483
0.556IleTrp: 0.556 ± 0.181
1.905IleTyr: 1.905 ± 0.487
0.0IleXaa: 0.0 ± 0.0
Lys
6.113LysAla: 6.113 ± 0.795
0.318LysCys: 0.318 ± 0.15
5.478LysAsp: 5.478 ± 0.701
8.812LysGlu: 8.812 ± 1.042
3.096LysPhe: 3.096 ± 0.442
4.763LysGly: 4.763 ± 0.826
1.508LysHis: 1.508 ± 0.379
4.525LysIle: 4.525 ± 0.632
5.954LysLys: 5.954 ± 0.784
6.907LysLeu: 6.907 ± 0.752
4.128LysMet: 4.128 ± 0.478
5.24LysAsn: 5.24 ± 0.708
3.255LysPro: 3.255 ± 0.531
3.97LysGln: 3.97 ± 0.534
3.97LysArg: 3.97 ± 0.518
4.208LysSer: 4.208 ± 0.913
5.16LysThr: 5.16 ± 0.545
5.875LysVal: 5.875 ± 0.576
1.191LysTrp: 1.191 ± 0.36
3.573LysTyr: 3.573 ± 0.511
0.0LysXaa: 0.0 ± 0.0
Leu
3.89LeuAla: 3.89 ± 0.58
0.635LeuCys: 0.635 ± 0.225
5.875LeuAsp: 5.875 ± 0.707
8.971LeuGlu: 8.971 ± 0.984
3.573LeuPhe: 3.573 ± 0.632
5.24LeuGly: 5.24 ± 0.927
1.111LeuHis: 1.111 ± 0.235
4.922LeuIle: 4.922 ± 0.727
7.383LeuLys: 7.383 ± 0.82
6.748LeuLeu: 6.748 ± 0.969
2.144LeuMet: 2.144 ± 0.411
4.922LeuAsn: 4.922 ± 0.692
2.62LeuPro: 2.62 ± 0.509
4.049LeuGln: 4.049 ± 0.474
2.382LeuArg: 2.382 ± 0.435
4.843LeuSer: 4.843 ± 0.637
4.128LeuThr: 4.128 ± 0.531
5.081LeuVal: 5.081 ± 0.627
0.953LeuTrp: 0.953 ± 0.25
2.699LeuTyr: 2.699 ± 0.551
0.0LeuXaa: 0.0 ± 0.0
Met
1.508MetAla: 1.508 ± 0.422
0.318MetCys: 0.318 ± 0.154
1.826MetAsp: 1.826 ± 0.386
2.223MetGlu: 2.223 ± 0.583
1.35MetPhe: 1.35 ± 0.401
2.064MetGly: 2.064 ± 0.506
0.079MetHis: 0.079 ± 0.074
2.382MetIle: 2.382 ± 0.413
3.017MetLys: 3.017 ± 0.488
2.144MetLeu: 2.144 ± 0.412
0.476MetMet: 0.476 ± 0.184
2.144MetAsn: 2.144 ± 0.395
0.476MetPro: 0.476 ± 0.18
0.953MetGln: 0.953 ± 0.256
1.826MetArg: 1.826 ± 0.392
1.826MetSer: 1.826 ± 0.448
2.302MetThr: 2.302 ± 0.386
1.985MetVal: 1.985 ± 0.446
0.556MetTrp: 0.556 ± 0.273
1.667MetTyr: 1.667 ± 0.439
0.0MetXaa: 0.0 ± 0.0
Asn
4.446AsnAla: 4.446 ± 0.908
0.159AsnCys: 0.159 ± 0.114
3.414AsnAsp: 3.414 ± 0.508
6.113AsnGlu: 6.113 ± 0.701
1.905AsnPhe: 1.905 ± 0.366
6.351AsnGly: 6.351 ± 0.622
0.873AsnHis: 0.873 ± 0.224
4.208AsnIle: 4.208 ± 0.623
6.272AsnLys: 6.272 ± 0.746
4.684AsnLeu: 4.684 ± 0.497
1.747AsnMet: 1.747 ± 0.404
3.573AsnAsn: 3.573 ± 0.571
1.588AsnPro: 1.588 ± 0.364
1.826AsnGln: 1.826 ± 0.358
1.27AsnArg: 1.27 ± 0.242
3.731AsnSer: 3.731 ± 0.582
4.446AsnThr: 4.446 ± 0.582
3.97AsnVal: 3.97 ± 0.533
0.715AsnTrp: 0.715 ± 0.241
2.699AsnTyr: 2.699 ± 0.53
0.0AsnXaa: 0.0 ± 0.0
Pro
1.985ProAla: 1.985 ± 0.445
0.0ProCys: 0.0 ± 0.0
2.302ProAsp: 2.302 ± 0.546
3.414ProGlu: 3.414 ± 0.599
1.111ProPhe: 1.111 ± 0.31
0.0ProGly: 0.0 ± 0.0
0.318ProHis: 0.318 ± 0.15
1.588ProIle: 1.588 ± 0.273
2.144ProLys: 2.144 ± 0.607
2.62ProLeu: 2.62 ± 0.443
0.794ProMet: 0.794 ± 0.22
1.985ProAsn: 1.985 ± 0.392
0.238ProPro: 0.238 ± 0.166
1.35ProGln: 1.35 ± 0.332
0.794ProArg: 0.794 ± 0.246
1.508ProSer: 1.508 ± 0.307
2.064ProThr: 2.064 ± 0.381
1.985ProVal: 1.985 ± 0.349
0.238ProTrp: 0.238 ± 0.128
1.588ProTyr: 1.588 ± 0.466
0.0ProXaa: 0.0 ± 0.0
Gln
2.461GlnAla: 2.461 ± 0.369
0.397GlnCys: 0.397 ± 0.178
1.905GlnAsp: 1.905 ± 0.313
2.699GlnGlu: 2.699 ± 0.456
1.588GlnPhe: 1.588 ± 0.288
1.747GlnGly: 1.747 ± 0.301
0.556GlnHis: 0.556 ± 0.204
2.699GlnIle: 2.699 ± 0.411
2.302GlnLys: 2.302 ± 0.46
3.096GlnLeu: 3.096 ± 0.455
1.508GlnMet: 1.508 ± 0.347
1.826GlnAsn: 1.826 ± 0.353
1.27GlnPro: 1.27 ± 0.449
2.223GlnGln: 2.223 ± 0.441
2.064GlnArg: 2.064 ± 0.454
2.223GlnSer: 2.223 ± 0.461
1.985GlnThr: 1.985 ± 0.288
2.779GlnVal: 2.779 ± 0.468
0.715GlnTrp: 0.715 ± 0.449
2.382GlnTyr: 2.382 ± 0.501
0.0GlnXaa: 0.0 ± 0.0
Arg
1.905ArgAla: 1.905 ± 0.371
0.318ArgCys: 0.318 ± 0.19
2.779ArgAsp: 2.779 ± 0.529
2.382ArgGlu: 2.382 ± 0.456
1.985ArgPhe: 1.985 ± 0.351
1.747ArgGly: 1.747 ± 0.476
0.715ArgHis: 0.715 ± 0.248
2.62ArgIle: 2.62 ± 0.437
3.017ArgLys: 3.017 ± 0.515
3.096ArgLeu: 3.096 ± 0.612
0.873ArgMet: 0.873 ± 0.249
2.382ArgAsn: 2.382 ± 0.426
0.953ArgPro: 0.953 ± 0.252
1.429ArgGln: 1.429 ± 0.308
1.191ArgArg: 1.191 ± 0.279
1.747ArgSer: 1.747 ± 0.414
1.747ArgThr: 1.747 ± 0.379
2.62ArgVal: 2.62 ± 0.495
0.238ArgTrp: 0.238 ± 0.164
1.667ArgTyr: 1.667 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
3.811SerAla: 3.811 ± 0.819
0.238SerCys: 0.238 ± 0.132
3.096SerAsp: 3.096 ± 0.479
3.652SerGlu: 3.652 ± 0.515
2.223SerPhe: 2.223 ± 0.307
4.763SerGly: 4.763 ± 0.798
1.667SerHis: 1.667 ± 0.397
3.334SerIle: 3.334 ± 0.462
4.366SerLys: 4.366 ± 0.538
3.573SerLeu: 3.573 ± 0.602
1.429SerMet: 1.429 ± 0.291
3.573SerAsn: 3.573 ± 0.587
1.111SerPro: 1.111 ± 0.268
2.064SerGln: 2.064 ± 0.455
1.35SerArg: 1.35 ± 0.392
2.858SerSer: 2.858 ± 0.65
3.652SerThr: 3.652 ± 0.731
2.382SerVal: 2.382 ± 0.42
0.794SerTrp: 0.794 ± 0.212
2.461SerTyr: 2.461 ± 0.613
0.0SerXaa: 0.0 ± 0.0
Thr
3.573ThrAla: 3.573 ± 0.45
0.318ThrCys: 0.318 ± 0.141
2.937ThrAsp: 2.937 ± 0.469
4.287ThrGlu: 4.287 ± 0.517
2.461ThrPhe: 2.461 ± 0.476
4.446ThrGly: 4.446 ± 0.655
1.429ThrHis: 1.429 ± 0.346
4.605ThrIle: 4.605 ± 0.614
6.986ThrLys: 6.986 ± 0.737
5.399ThrLeu: 5.399 ± 0.829
1.667ThrMet: 1.667 ± 0.408
3.414ThrAsn: 3.414 ± 0.629
2.302ThrPro: 2.302 ± 0.402
2.144ThrGln: 2.144 ± 0.371
2.223ThrArg: 2.223 ± 0.394
2.144ThrSer: 2.144 ± 0.418
4.605ThrThr: 4.605 ± 0.927
4.366ThrVal: 4.366 ± 0.608
0.635ThrTrp: 0.635 ± 0.212
2.382ThrTyr: 2.382 ± 0.526
0.0ThrXaa: 0.0 ± 0.0
Val
4.843ValAla: 4.843 ± 0.534
0.556ValCys: 0.556 ± 0.216
4.605ValAsp: 4.605 ± 0.572
5.557ValGlu: 5.557 ± 0.815
2.937ValPhe: 2.937 ± 0.43
3.731ValGly: 3.731 ± 0.538
0.556ValHis: 0.556 ± 0.216
4.128ValIle: 4.128 ± 0.531
5.716ValLys: 5.716 ± 0.884
5.478ValLeu: 5.478 ± 0.691
1.985ValMet: 1.985 ± 0.297
4.287ValAsn: 4.287 ± 0.515
1.905ValPro: 1.905 ± 0.323
2.302ValGln: 2.302 ± 0.568
2.54ValArg: 2.54 ± 0.442
4.843ValSer: 4.843 ± 0.493
3.573ValThr: 3.573 ± 0.548
5.24ValVal: 5.24 ± 0.565
0.794ValTrp: 0.794 ± 0.248
2.461ValTyr: 2.461 ± 0.479
0.0ValXaa: 0.0 ± 0.0
Trp
0.476TrpAla: 0.476 ± 0.194
0.238TrpCys: 0.238 ± 0.154
0.556TrpAsp: 0.556 ± 0.233
1.667TrpGlu: 1.667 ± 0.311
0.953TrpPhe: 0.953 ± 0.209
1.111TrpGly: 1.111 ± 0.232
0.079TrpHis: 0.079 ± 0.072
0.556TrpIle: 0.556 ± 0.231
0.635TrpLys: 0.635 ± 0.195
1.032TrpLeu: 1.032 ± 0.289
0.159TrpMet: 0.159 ± 0.116
0.953TrpAsn: 0.953 ± 0.288
0.0TrpPro: 0.0 ± 0.0
0.397TrpGln: 0.397 ± 0.169
0.556TrpArg: 0.556 ± 0.269
0.556TrpSer: 0.556 ± 0.169
0.635TrpThr: 0.635 ± 0.188
1.35TrpVal: 1.35 ± 0.233
0.318TrpTrp: 0.318 ± 0.123
0.238TrpTyr: 0.238 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.432
0.476TyrCys: 0.476 ± 0.165
3.493TyrAsp: 3.493 ± 0.604
3.652TyrGlu: 3.652 ± 0.618
1.905TyrPhe: 1.905 ± 0.295
1.905TyrGly: 1.905 ± 0.428
0.556TyrHis: 0.556 ± 0.17
3.414TyrIle: 3.414 ± 0.583
4.049TyrLys: 4.049 ± 0.691
3.731TyrLeu: 3.731 ± 0.651
1.032TyrMet: 1.032 ± 0.238
2.937TyrAsn: 2.937 ± 0.482
1.35TyrPro: 1.35 ± 0.35
1.111TyrGln: 1.111 ± 0.272
0.794TyrArg: 0.794 ± 0.255
1.826TyrSer: 1.826 ± 0.448
2.54TyrThr: 2.54 ± 0.551
2.461TyrVal: 2.461 ± 0.486
0.159TyrTrp: 0.159 ± 0.094
1.667TyrTyr: 1.667 ± 0.476
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12597 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski