Staphylococcus phage phiSP119-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499SR17|A0A499SR17_9CAUD Major capsid protein OS=Staphylococcus phage phiSP119-3 OX=2491318 PE=4 SV=1
MM1 pKa = 7.69IKK3 pKa = 9.31RR4 pKa = 11.84TINLLITLALYY15 pKa = 10.26EE16 pKa = 4.56LGTYY20 pKa = 8.07LTEE23 pKa = 3.71QAIIILTANDD33 pKa = 3.79DD34 pKa = 3.21VDD36 pKa = 4.36AFNEE40 pKa = 3.91YY41 pKa = 11.24DD42 pKa = 4.95HH43 pKa = 7.44IDD45 pKa = 3.72LNDD48 pKa = 2.84IRR50 pKa = 11.84AEE52 pKa = 4.05VSGG55 pKa = 3.91

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499SJW5|A0A499SJW5_9CAUD VRR-NUC domain-containing protein OS=Staphylococcus phage phiSP119-3 OX=2491318 PE=4 SV=1
MM1 pKa = 7.28ATNPPKK7 pKa = 10.54DD8 pKa = 3.54GRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.63GAVKK16 pKa = 10.41SRR18 pKa = 11.84SQVKK22 pKa = 9.45NPKK25 pKa = 6.99TNRR28 pKa = 11.84YY29 pKa = 7.67VKK31 pKa = 10.3RR32 pKa = 11.84DD33 pKa = 3.45TKK35 pKa = 10.27TGRR38 pKa = 11.84FMDD41 pKa = 3.97MKK43 pKa = 10.74SDD45 pKa = 3.6SKK47 pKa = 10.75PFKK50 pKa = 10.45GVRR53 pKa = 11.84KK54 pKa = 8.89EE55 pKa = 3.86HH56 pKa = 6.25

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13527

41

1039

198.9

22.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.862 ± 0.367

0.525 ± 0.096

6.616 ± 0.365

7.54 ± 0.462

4.295 ± 0.176

5.264 ± 0.242

1.893 ± 0.141

7.348 ± 0.301

9.492 ± 0.333

8.258 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.117

6.735 ± 0.252

2.454 ± 0.183

3.608 ± 0.18

4.177 ± 0.212

5.877 ± 0.287

6.069 ± 0.237

6.335 ± 0.263

1.072 ± 0.139

4.295 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski