Amino acid dipepetide frequency for Microbacterium phage Phractured

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.216AlaAla: 11.216 ± 1.261
0.294AlaCys: 0.294 ± 0.17
7.047AlaAsp: 7.047 ± 0.525
6.988AlaGlu: 6.988 ± 0.746
2.76AlaPhe: 2.76 ± 0.409
6.283AlaGly: 6.283 ± 0.712
1.351AlaHis: 1.351 ± 0.301
4.052AlaIle: 4.052 ± 0.378
2.877AlaLys: 2.877 ± 0.417
7.751AlaLeu: 7.751 ± 0.492
2.231AlaMet: 2.231 ± 0.352
4.404AlaAsn: 4.404 ± 0.555
3.993AlaPro: 3.993 ± 0.417
4.991AlaGln: 4.991 ± 0.77
6.166AlaArg: 6.166 ± 0.578
6.225AlaSer: 6.225 ± 0.558
5.872AlaThr: 5.872 ± 0.587
5.872AlaVal: 5.872 ± 0.655
1.703AlaTrp: 1.703 ± 0.334
3.289AlaTyr: 3.289 ± 0.361
0.0AlaXaa: 0.0 ± 0.0
Cys
0.235CysAla: 0.235 ± 0.137
0.0CysCys: 0.0 ± 0.0
0.059CysAsp: 0.059 ± 0.063
0.294CysGlu: 0.294 ± 0.162
0.352CysPhe: 0.352 ± 0.186
0.47CysGly: 0.47 ± 0.196
0.235CysHis: 0.235 ± 0.133
0.176CysIle: 0.176 ± 0.151
0.176CysLys: 0.176 ± 0.12
0.411CysLeu: 0.411 ± 0.18
0.176CysMet: 0.176 ± 0.117
0.117CysAsn: 0.117 ± 0.096
0.411CysPro: 0.411 ± 0.251
0.059CysGln: 0.059 ± 0.071
0.176CysArg: 0.176 ± 0.111
0.352CysSer: 0.352 ± 0.183
0.294CysThr: 0.294 ± 0.139
0.47CysVal: 0.47 ± 0.208
0.059CysTrp: 0.059 ± 0.063
0.176CysTyr: 0.176 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
6.871AspAla: 6.871 ± 0.969
0.411AspCys: 0.411 ± 0.202
3.993AspAsp: 3.993 ± 0.413
5.755AspGlu: 5.755 ± 0.602
2.055AspPhe: 2.055 ± 0.338
5.579AspGly: 5.579 ± 0.498
1.174AspHis: 1.174 ± 0.248
3.406AspIle: 3.406 ± 0.409
3.758AspLys: 3.758 ± 0.578
5.52AspLeu: 5.52 ± 0.538
1.527AspMet: 1.527 ± 0.301
2.643AspAsn: 2.643 ± 0.331
3.289AspPro: 3.289 ± 0.487
2.995AspGln: 2.995 ± 0.409
3.582AspArg: 3.582 ± 0.458
2.995AspSer: 2.995 ± 0.366
3.758AspThr: 3.758 ± 0.413
3.23AspVal: 3.23 ± 0.392
1.879AspTrp: 1.879 ± 0.301
1.644AspTyr: 1.644 ± 0.311
0.0AspXaa: 0.0 ± 0.0
Glu
6.342GluAla: 6.342 ± 0.559
0.059GluCys: 0.059 ± 0.055
4.698GluAsp: 4.698 ± 0.693
3.993GluGlu: 3.993 ± 0.692
2.055GluPhe: 2.055 ± 0.297
4.757GluGly: 4.757 ± 0.718
1.116GluHis: 1.116 ± 0.305
2.76GluIle: 2.76 ± 0.463
3.23GluLys: 3.23 ± 0.51
7.047GluLeu: 7.047 ± 0.659
1.703GluMet: 1.703 ± 0.322
3.112GluAsn: 3.112 ± 0.396
1.938GluPro: 1.938 ± 0.392
3.289GluGln: 3.289 ± 0.378
4.052GluArg: 4.052 ± 0.662
3.171GluSer: 3.171 ± 0.413
4.169GluThr: 4.169 ± 0.508
4.404GluVal: 4.404 ± 0.497
1.997GluTrp: 1.997 ± 0.226
2.29GluTyr: 2.29 ± 0.511
0.0GluXaa: 0.0 ± 0.0
Phe
2.29PheAla: 2.29 ± 0.341
0.529PheCys: 0.529 ± 0.236
2.584PheAsp: 2.584 ± 0.332
2.114PheGlu: 2.114 ± 0.499
1.586PhePhe: 1.586 ± 0.313
2.349PheGly: 2.349 ± 0.323
0.352PheHis: 0.352 ± 0.12
1.938PheIle: 1.938 ± 0.375
1.292PheLys: 1.292 ± 0.315
1.703PheLeu: 1.703 ± 0.315
0.94PheMet: 0.94 ± 0.284
1.703PheAsn: 1.703 ± 0.324
1.233PhePro: 1.233 ± 0.362
1.233PheGln: 1.233 ± 0.216
2.114PheArg: 2.114 ± 0.3
2.525PheSer: 2.525 ± 0.284
2.408PheThr: 2.408 ± 0.387
2.173PheVal: 2.173 ± 0.309
0.47PheTrp: 0.47 ± 0.16
0.763PheTyr: 0.763 ± 0.161
0.0PheXaa: 0.0 ± 0.0
Gly
7.047GlyAla: 7.047 ± 0.823
0.117GlyCys: 0.117 ± 0.093
4.58GlyAsp: 4.58 ± 0.472
3.641GlyGlu: 3.641 ± 0.384
2.29GlyPhe: 2.29 ± 0.288
7.282GlyGly: 7.282 ± 1.092
0.881GlyHis: 0.881 ± 0.222
3.993GlyIle: 3.993 ± 0.489
3.347GlyLys: 3.347 ± 0.723
7.047GlyLeu: 7.047 ± 0.847
2.055GlyMet: 2.055 ± 0.499
3.054GlyAsn: 3.054 ± 0.433
1.82GlyPro: 1.82 ± 0.235
3.054GlyGln: 3.054 ± 0.523
4.522GlyArg: 4.522 ± 0.664
5.637GlySer: 5.637 ± 0.634
4.815GlyThr: 4.815 ± 0.487
5.344GlyVal: 5.344 ± 0.592
1.468GlyTrp: 1.468 ± 0.204
2.995GlyTyr: 2.995 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
1.292HisAla: 1.292 ± 0.195
0.294HisCys: 0.294 ± 0.146
1.174HisAsp: 1.174 ± 0.258
1.057HisGlu: 1.057 ± 0.346
0.411HisPhe: 0.411 ± 0.149
1.409HisGly: 1.409 ± 0.321
0.411HisHis: 0.411 ± 0.177
0.763HisIle: 0.763 ± 0.225
1.233HisLys: 1.233 ± 0.359
0.998HisLeu: 0.998 ± 0.18
0.352HisMet: 0.352 ± 0.215
0.94HisAsn: 0.94 ± 0.266
1.351HisPro: 1.351 ± 0.284
0.47HisGln: 0.47 ± 0.133
0.881HisArg: 0.881 ± 0.232
0.94HisSer: 0.94 ± 0.363
0.646HisThr: 0.646 ± 0.206
1.233HisVal: 1.233 ± 0.324
0.411HisTrp: 0.411 ± 0.136
0.294HisTyr: 0.294 ± 0.104
0.0HisXaa: 0.0 ± 0.0
Ile
5.05IleAla: 5.05 ± 0.489
0.117IleCys: 0.117 ± 0.08
4.052IleAsp: 4.052 ± 0.509
3.465IleGlu: 3.465 ± 0.507
1.292IlePhe: 1.292 ± 0.285
3.289IleGly: 3.289 ± 0.312
0.998IleHis: 0.998 ± 0.179
3.054IleIle: 3.054 ± 0.473
3.054IleLys: 3.054 ± 0.532
3.641IleLeu: 3.641 ± 0.412
1.116IleMet: 1.116 ± 0.207
2.701IleAsn: 2.701 ± 0.33
2.173IlePro: 2.173 ± 0.42
1.997IleGln: 1.997 ± 0.361
2.936IleArg: 2.936 ± 0.359
2.584IleSer: 2.584 ± 0.338
3.465IleThr: 3.465 ± 0.468
2.995IleVal: 2.995 ± 0.485
0.411IleTrp: 0.411 ± 0.162
1.057IleTyr: 1.057 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
4.052LysAla: 4.052 ± 0.545
0.352LysCys: 0.352 ± 0.157
2.877LysAsp: 2.877 ± 0.456
2.466LysGlu: 2.466 ± 0.458
1.292LysPhe: 1.292 ± 0.203
2.936LysGly: 2.936 ± 0.296
0.47LysHis: 0.47 ± 0.176
2.349LysIle: 2.349 ± 0.336
2.525LysLys: 2.525 ± 0.547
3.876LysLeu: 3.876 ± 0.661
1.174LysMet: 1.174 ± 0.359
1.586LysAsn: 1.586 ± 0.265
2.349LysPro: 2.349 ± 0.393
1.82LysGln: 1.82 ± 0.331
2.173LysArg: 2.173 ± 0.475
2.643LysSer: 2.643 ± 0.435
3.054LysThr: 3.054 ± 0.515
3.289LysVal: 3.289 ± 0.466
0.646LysTrp: 0.646 ± 0.185
2.055LysTyr: 2.055 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
7.634LeuAla: 7.634 ± 0.587
0.294LeuCys: 0.294 ± 0.179
5.285LeuAsp: 5.285 ± 0.822
5.637LeuGlu: 5.637 ± 0.654
2.584LeuPhe: 2.584 ± 0.301
5.872LeuGly: 5.872 ± 0.537
1.409LeuHis: 1.409 ± 0.365
4.698LeuIle: 4.698 ± 0.64
3.582LeuLys: 3.582 ± 0.699
6.401LeuLeu: 6.401 ± 0.733
1.938LeuMet: 1.938 ± 0.333
4.111LeuAsn: 4.111 ± 0.399
3.876LeuPro: 3.876 ± 0.546
4.346LeuGln: 4.346 ± 0.476
5.403LeuArg: 5.403 ± 0.48
5.168LeuSer: 5.168 ± 0.496
5.814LeuThr: 5.814 ± 0.629
5.285LeuVal: 5.285 ± 0.586
1.174LeuTrp: 1.174 ± 0.16
2.466LeuTyr: 2.466 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
2.936MetAla: 2.936 ± 0.352
0.059MetCys: 0.059 ± 0.062
1.409MetAsp: 1.409 ± 0.243
1.703MetGlu: 1.703 ± 0.312
1.057MetPhe: 1.057 ± 0.228
1.233MetGly: 1.233 ± 0.239
0.529MetHis: 0.529 ± 0.134
0.822MetIle: 0.822 ± 0.233
0.822MetLys: 0.822 ± 0.278
1.938MetLeu: 1.938 ± 0.365
0.763MetMet: 0.763 ± 0.21
1.174MetAsn: 1.174 ± 0.303
1.527MetPro: 1.527 ± 0.266
1.116MetGln: 1.116 ± 0.232
1.586MetArg: 1.586 ± 0.238
2.055MetSer: 2.055 ± 0.38
2.114MetThr: 2.114 ± 0.319
1.409MetVal: 1.409 ± 0.278
0.294MetTrp: 0.294 ± 0.123
0.705MetTyr: 0.705 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
4.169AsnAla: 4.169 ± 0.492
0.176AsnCys: 0.176 ± 0.121
2.466AsnAsp: 2.466 ± 0.314
2.525AsnGlu: 2.525 ± 0.346
1.644AsnPhe: 1.644 ± 0.223
3.993AsnGly: 3.993 ± 0.437
0.235AsnHis: 0.235 ± 0.142
2.29AsnIle: 2.29 ± 0.325
1.997AsnLys: 1.997 ± 0.359
2.877AsnLeu: 2.877 ± 0.357
0.822AsnMet: 0.822 ± 0.231
2.76AsnAsn: 2.76 ± 0.419
3.289AsnPro: 3.289 ± 0.445
1.938AsnGln: 1.938 ± 0.345
2.173AsnArg: 2.173 ± 0.318
2.819AsnSer: 2.819 ± 0.363
3.523AsnThr: 3.523 ± 0.436
3.934AsnVal: 3.934 ± 0.432
0.998AsnTrp: 0.998 ± 0.235
1.762AsnTyr: 1.762 ± 0.369
0.0AsnXaa: 0.0 ± 0.0
Pro
4.463ProAla: 4.463 ± 0.613
0.059ProCys: 0.059 ± 0.061
3.347ProAsp: 3.347 ± 0.436
4.933ProGlu: 4.933 ± 0.57
1.351ProPhe: 1.351 ± 0.289
3.171ProGly: 3.171 ± 0.473
0.705ProHis: 0.705 ± 0.217
2.055ProIle: 2.055 ± 0.442
2.055ProLys: 2.055 ± 0.356
2.76ProLeu: 2.76 ± 0.349
1.174ProMet: 1.174 ± 0.269
2.173ProAsn: 2.173 ± 0.362
1.351ProPro: 1.351 ± 0.338
1.468ProGln: 1.468 ± 0.205
2.114ProArg: 2.114 ± 0.403
3.876ProSer: 3.876 ± 0.513
3.289ProThr: 3.289 ± 0.536
2.936ProVal: 2.936 ± 0.296
1.233ProTrp: 1.233 ± 0.254
1.351ProTyr: 1.351 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
5.52GlnAla: 5.52 ± 0.584
0.352GlnCys: 0.352 ± 0.166
2.584GlnAsp: 2.584 ± 0.353
2.877GlnGlu: 2.877 ± 0.381
1.116GlnPhe: 1.116 ± 0.176
2.525GlnGly: 2.525 ± 0.433
1.057GlnHis: 1.057 ± 0.184
2.584GlnIle: 2.584 ± 0.339
1.703GlnLys: 1.703 ± 0.309
3.582GlnLeu: 3.582 ± 0.373
1.586GlnMet: 1.586 ± 0.338
2.29GlnAsn: 2.29 ± 0.295
1.82GlnPro: 1.82 ± 0.367
2.231GlnGln: 2.231 ± 0.438
2.819GlnArg: 2.819 ± 0.418
2.231GlnSer: 2.231 ± 0.369
3.758GlnThr: 3.758 ± 1.036
2.231GlnVal: 2.231 ± 0.408
0.822GlnTrp: 0.822 ± 0.259
1.409GlnTyr: 1.409 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
5.05ArgAla: 5.05 ± 0.686
0.235ArgCys: 0.235 ± 0.167
3.934ArgAsp: 3.934 ± 0.665
4.522ArgGlu: 4.522 ± 0.587
2.466ArgPhe: 2.466 ± 0.452
4.933ArgGly: 4.933 ± 0.703
0.881ArgHis: 0.881 ± 0.207
3.112ArgIle: 3.112 ± 0.515
1.879ArgLys: 1.879 ± 0.345
4.874ArgLeu: 4.874 ± 0.428
2.408ArgMet: 2.408 ± 0.534
3.054ArgAsn: 3.054 ± 0.356
2.466ArgPro: 2.466 ± 0.34
3.582ArgGln: 3.582 ± 0.478
3.641ArgArg: 3.641 ± 0.833
3.465ArgSer: 3.465 ± 0.597
3.465ArgThr: 3.465 ± 0.472
3.934ArgVal: 3.934 ± 0.443
0.763ArgTrp: 0.763 ± 0.22
1.233ArgTyr: 1.233 ± 0.215
0.0ArgXaa: 0.0 ± 0.0
Ser
5.872SerAla: 5.872 ± 0.574
0.411SerCys: 0.411 ± 0.17
3.876SerAsp: 3.876 ± 0.564
3.641SerGlu: 3.641 ± 0.374
2.114SerPhe: 2.114 ± 0.383
5.226SerGly: 5.226 ± 0.709
1.174SerHis: 1.174 ± 0.295
3.406SerIle: 3.406 ± 0.512
1.997SerLys: 1.997 ± 0.215
5.637SerLeu: 5.637 ± 0.691
1.938SerMet: 1.938 ± 0.394
2.584SerAsn: 2.584 ± 0.459
2.819SerPro: 2.819 ± 0.284
2.408SerGln: 2.408 ± 0.455
3.523SerArg: 3.523 ± 0.402
4.228SerSer: 4.228 ± 0.768
4.815SerThr: 4.815 ± 0.471
3.641SerVal: 3.641 ± 0.39
0.881SerTrp: 0.881 ± 0.332
2.055SerTyr: 2.055 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
6.636ThrAla: 6.636 ± 0.724
0.235ThrCys: 0.235 ± 0.121
3.465ThrAsp: 3.465 ± 0.352
4.169ThrGlu: 4.169 ± 0.454
2.466ThrPhe: 2.466 ± 0.278
6.46ThrGly: 6.46 ± 0.933
1.174ThrHis: 1.174 ± 0.344
2.701ThrIle: 2.701 ± 0.315
2.408ThrLys: 2.408 ± 0.334
6.577ThrLeu: 6.577 ± 0.555
0.763ThrMet: 0.763 ± 0.17
3.112ThrAsn: 3.112 ± 0.36
4.58ThrPro: 4.58 ± 0.568
2.584ThrGln: 2.584 ± 0.433
4.287ThrArg: 4.287 ± 0.491
4.111ThrSer: 4.111 ± 0.507
4.757ThrThr: 4.757 ± 0.513
4.757ThrVal: 4.757 ± 0.623
0.881ThrTrp: 0.881 ± 0.241
1.938ThrTyr: 1.938 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
5.696ValAla: 5.696 ± 0.582
0.294ValCys: 0.294 ± 0.141
5.109ValAsp: 5.109 ± 0.634
3.465ValGlu: 3.465 ± 0.528
1.762ValPhe: 1.762 ± 0.267
3.817ValGly: 3.817 ± 0.457
1.351ValHis: 1.351 ± 0.271
2.877ValIle: 2.877 ± 0.377
3.112ValLys: 3.112 ± 0.686
5.696ValLeu: 5.696 ± 0.526
1.527ValMet: 1.527 ± 0.376
2.643ValAsn: 2.643 ± 0.546
4.111ValPro: 4.111 ± 0.612
2.936ValGln: 2.936 ± 0.316
4.757ValArg: 4.757 ± 0.392
4.287ValSer: 4.287 ± 0.646
4.874ValThr: 4.874 ± 0.543
3.934ValVal: 3.934 ± 0.516
1.409ValTrp: 1.409 ± 0.298
1.409ValTyr: 1.409 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
1.057TrpAla: 1.057 ± 0.227
0.176TrpCys: 0.176 ± 0.106
1.468TrpAsp: 1.468 ± 0.283
0.705TrpGlu: 0.705 ± 0.264
0.646TrpPhe: 0.646 ± 0.198
1.233TrpGly: 1.233 ± 0.367
0.352TrpHis: 0.352 ± 0.133
1.116TrpIle: 1.116 ± 0.337
1.233TrpLys: 1.233 ± 0.273
1.703TrpLeu: 1.703 ± 0.352
0.294TrpMet: 0.294 ± 0.135
0.822TrpAsn: 0.822 ± 0.164
0.47TrpPro: 0.47 ± 0.143
1.057TrpGln: 1.057 ± 0.2
1.116TrpArg: 1.116 ± 0.29
1.057TrpSer: 1.057 ± 0.424
1.586TrpThr: 1.586 ± 0.269
1.116TrpVal: 1.116 ± 0.246
0.411TrpTrp: 0.411 ± 0.138
0.822TrpTyr: 0.822 ± 0.296
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.997TyrAla: 1.997 ± 0.405
0.235TyrCys: 0.235 ± 0.13
2.055TyrAsp: 2.055 ± 0.239
2.231TyrGlu: 2.231 ± 0.395
0.998TyrPhe: 0.998 ± 0.32
2.173TyrGly: 2.173 ± 0.41
0.705TyrHis: 0.705 ± 0.224
1.292TyrIle: 1.292 ± 0.189
1.703TyrLys: 1.703 ± 0.319
2.936TyrLeu: 2.936 ± 0.297
0.705TyrMet: 0.705 ± 0.174
1.409TyrAsn: 1.409 ± 0.291
0.998TyrPro: 0.998 ± 0.324
1.527TyrGln: 1.527 ± 0.22
1.879TyrArg: 1.879 ± 0.3
1.879TyrSer: 1.879 ± 0.323
1.586TyrThr: 1.586 ± 0.419
2.877TyrVal: 2.877 ± 0.517
0.529TyrTrp: 0.529 ± 0.156
1.409TyrTyr: 1.409 ± 0.323
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (17030 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski