Microbacterium phage GaeCeo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Schubertvirus; unclassified Schubertvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9A319|A0A7G9A319_9CAUD DUF2786 domain-containing protein OS=Microbacterium phage GaeCeo OX=2759461 GN=55 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.12NNYY5 pKa = 8.3PTFQEE10 pKa = 3.88VRR12 pKa = 11.84NYY14 pKa = 10.92LIDD17 pKa = 4.91DD18 pKa = 4.21NDD20 pKa = 3.8WTDD23 pKa = 3.52EE24 pKa = 4.34EE25 pKa = 4.58VDD27 pKa = 3.58QLAGYY32 pKa = 9.83AFRR35 pKa = 11.84HH36 pKa = 5.65INQDD40 pKa = 2.44GTTTMTRR47 pKa = 11.84DD48 pKa = 2.59QWTCYY53 pKa = 10.12IMDD56 pKa = 3.99YY57 pKa = 10.69CNEE60 pKa = 4.55HH61 pKa = 6.81IDD63 pKa = 3.8LL64 pKa = 4.85

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9A2Y7|A0A7G9A2Y7_9CAUD Uncharacterized protein OS=Microbacterium phage GaeCeo OX=2759461 GN=22 PE=4 SV=1
MM1 pKa = 7.62AMRR4 pKa = 11.84RR5 pKa = 11.84AIVRR9 pKa = 11.84NPNSFKK15 pKa = 9.73QTLTIQVKK23 pKa = 7.7VTSAGGDD30 pKa = 3.37VKK32 pKa = 10.4ITDD35 pKa = 5.86LILQAGSTGTGWVPNVTEE53 pKa = 4.44MPWTAGVVSS62 pKa = 4.18

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12836

50

808

221.3

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.058 ± 0.432

0.639 ± 0.093

6.614 ± 0.367

6.529 ± 0.475

3.077 ± 0.195

8.04 ± 0.401

1.893 ± 0.219

4.659 ± 0.437

4.908 ± 0.461

8.609 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.131

3.373 ± 0.196

4.503 ± 0.224

3.965 ± 0.178

5.726 ± 0.398

5.905 ± 0.323

6.661 ± 0.325

7.495 ± 0.303

1.987 ± 0.251

2.89 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski