Amino acid dipepetide frequency for Variovorax sp. HW608

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.034AlaAla: 18.034 ± 0.117	1.367AlaCys: 1.367 ± 0.029	6.393AlaAsp: 6.393 ± 0.054	6.896AlaGlu: 6.896 ± 0.06	4.295AlaPhe: 4.295 ± 0.044	10.94AlaGly: 10.94 ± 0.077	2.659AlaHis: 2.659 ± 0.034	5.715AlaIle: 5.715 ± 0.055	3.982AlaLys: 3.982 ± 0.049	14.459AlaLeu: 14.459 ± 0.101	3.553AlaMet: 3.553 ± 0.04	2.934AlaAsn: 2.934 ± 0.043	6.261AlaPro: 6.261 ± 0.065	5.236AlaGln: 5.236 ± 0.06	9.418AlaArg: 9.418 ± 0.08	7.225AlaSer: 7.225 ± 0.057	6.149AlaThr: 6.149 ± 0.052	9.188AlaVal: 9.188 ± 0.065	1.81AlaTrp: 1.81 ± 0.035	2.466AlaTyr: 2.466 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.232CysAla: 1.232 ± 0.023	0.137CysCys: 0.137 ± 0.008	0.575CysAsp: 0.575 ± 0.018	0.59CysGlu: 0.59 ± 0.016	0.335CysPhe: 0.335 ± 0.013	1.034CysGly: 1.034 ± 0.024	0.262CysHis: 0.262 ± 0.012	0.439CysIle: 0.439 ± 0.015	0.233CysLys: 0.233 ± 0.011	0.782CysLeu: 0.782 ± 0.019	0.213CysMet: 0.213 ± 0.01	0.259CysAsn: 0.259 ± 0.011	0.475CysPro: 0.475 ± 0.016	0.25CysGln: 0.25 ± 0.012	0.619CysArg: 0.619 ± 0.018	0.527CysSer: 0.527 ± 0.017	0.506CysThr: 0.506 ± 0.015	0.723CysVal: 0.723 ± 0.018	0.121CysTrp: 0.121 ± 0.007	0.195CysTyr: 0.195 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.404AspAla: 7.404 ± 0.067	0.481AspCys: 0.481 ± 0.018	2.635AspAsp: 2.635 ± 0.038	3.185AspGlu: 3.185 ± 0.039	2.02AspPhe: 2.02 ± 0.028	4.539AspGly: 4.539 ± 0.055	1.138AspHis: 1.138 ± 0.025	2.376AspIle: 2.376 ± 0.032	1.572AspLys: 1.572 ± 0.028	5.337AspLeu: 5.337 ± 0.046	1.162AspMet: 1.162 ± 0.022	1.162AspAsn: 1.162 ± 0.024	3.087AspPro: 3.087 ± 0.041	1.45AspGln: 1.45 ± 0.027	3.618AspArg: 3.618 ± 0.041	2.301AspSer: 2.301 ± 0.033	2.357AspThr: 2.357 ± 0.032	4.051AspVal: 4.051 ± 0.038	0.915AspTrp: 0.915 ± 0.021	1.335AspTyr: 1.335 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.165GluAla: 7.165 ± 0.071	0.432GluCys: 0.432 ± 0.014	2.26GluAsp: 2.26 ± 0.034	2.679GluGlu: 2.679 ± 0.047	1.855GluPhe: 1.855 ± 0.033	3.99GluGly: 3.99 ± 0.051	1.387GluHis: 1.387 ± 0.027	2.74GluIle: 2.74 ± 0.04	1.822GluLys: 1.822 ± 0.027	6.046GluLeu: 6.046 ± 0.058	1.307GluMet: 1.307 ± 0.023	1.236GluAsn: 1.236 ± 0.025	2.631GluPro: 2.631 ± 0.034	2.3GluGln: 2.3 ± 0.031	4.964GluArg: 4.964 ± 0.053	2.813GluSer: 2.813 ± 0.04	2.53GluThr: 2.53 ± 0.034	4.089GluVal: 4.089 ± 0.042	0.751GluTrp: 0.751 ± 0.019	1.006GluTyr: 1.006 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	4.464PheAla: 4.464 ± 0.05	0.422PheCys: 0.422 ± 0.014	2.59PheAsp: 2.59 ± 0.038	2.225PheGlu: 2.225 ± 0.033	1.331PhePhe: 1.331 ± 0.027	3.529PheGly: 3.529 ± 0.038	0.749PheHis: 0.749 ± 0.019	1.551PheIle: 1.551 ± 0.028	1.104PheLys: 1.104 ± 0.021	2.982PheLeu: 2.982 ± 0.041	0.819PheMet: 0.819 ± 0.018	1.083PheAsn: 1.083 ± 0.021	1.557PhePro: 1.557 ± 0.024	1.002PheGln: 1.002 ± 0.019	2.029PheArg: 2.029 ± 0.031	2.081PheSer: 2.081 ± 0.031	1.828PheThr: 1.828 ± 0.033	2.954PheVal: 2.954 ± 0.042	0.53PheTrp: 0.53 ± 0.015	0.807PheTyr: 0.807 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	9.632GlyAla: 9.632 ± 0.075	0.901GlyCys: 0.901 ± 0.019	4.028GlyAsp: 4.028 ± 0.044	4.456GlyGlu: 4.456 ± 0.046	3.434GlyPhe: 3.434 ± 0.037	7.425GlyGly: 7.425 ± 0.081	1.97GlyHis: 1.97 ± 0.031	4.338GlyIle: 4.338 ± 0.047	3.224GlyLys: 3.224 ± 0.049	8.612GlyLeu: 8.612 ± 0.076	2.326GlyMet: 2.326 ± 0.035	2.206GlyAsn: 2.206 ± 0.029	3.407GlyPro: 3.407 ± 0.038	3.038GlyGln: 3.038 ± 0.032	5.888GlyArg: 5.888 ± 0.056	4.919GlySer: 4.919 ± 0.055	4.644GlyThr: 4.644 ± 0.065	6.706GlyVal: 6.706 ± 0.064	1.457GlyTrp: 1.457 ± 0.03	2.292GlyTyr: 2.292 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.902HisAla: 2.902 ± 0.035	0.304HisCys: 0.304 ± 0.011	1.19HisAsp: 1.19 ± 0.026	1.219HisGlu: 1.219 ± 0.022	0.871HisPhe: 0.871 ± 0.021	2.166HisGly: 2.166 ± 0.037	0.61HisHis: 0.61 ± 0.017	0.909HisIle: 0.909 ± 0.021	0.518HisLys: 0.518 ± 0.014	2.2HisLeu: 2.2 ± 0.035	0.552HisMet: 0.552 ± 0.016	0.486HisAsn: 0.486 ± 0.015	1.537HisPro: 1.537 ± 0.024	0.657HisGln: 0.657 ± 0.019	1.645HisArg: 1.645 ± 0.028	1.057HisSer: 1.057 ± 0.022	0.972HisThr: 0.972 ± 0.021	1.696HisVal: 1.696 ± 0.027	0.388HisTrp: 0.388 ± 0.013	0.58HisTyr: 0.58 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.694IleAla: 6.694 ± 0.054	0.459IleCys: 0.459 ± 0.013	3.281IleAsp: 3.281 ± 0.039	3.319IleGlu: 3.319 ± 0.038	1.298IlePhe: 1.298 ± 0.023	4.554IleGly: 4.554 ± 0.046	0.914IleHis: 0.914 ± 0.022	1.425IleIle: 1.425 ± 0.027	1.353IleLys: 1.353 ± 0.028	3.283IleLeu: 3.283 ± 0.039	0.72IleMet: 0.72 ± 0.017	1.35IleAsn: 1.35 ± 0.025	2.105IlePro: 2.105 ± 0.032	1.274IleGln: 1.274 ± 0.023	2.796IleArg: 2.796 ± 0.037	2.361IleSer: 2.361 ± 0.031	2.304IleThr: 2.304 ± 0.035	3.893IleVal: 3.893 ± 0.044	0.493IleTrp: 0.493 ± 0.016	0.993IleTyr: 0.993 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.078LysAla: 4.078 ± 0.047	0.167LysCys: 0.167 ± 0.009	1.615LysAsp: 1.615 ± 0.031	1.561LysGlu: 1.561 ± 0.029	0.907LysPhe: 0.907 ± 0.022	2.43LysGly: 2.43 ± 0.032	0.614LysHis: 0.614 ± 0.016	1.414LysIle: 1.414 ± 0.029	1.253LysLys: 1.253 ± 0.035	3.49LysLeu: 3.49 ± 0.046	0.728LysMet: 0.728 ± 0.019	0.849LysAsn: 0.849 ± 0.022	2.005LysPro: 2.005 ± 0.029	1.094LysGln: 1.094 ± 0.023	2.248LysArg: 2.248 ± 0.036	1.819LysSer: 1.819 ± 0.028	1.829LysThr: 1.829 ± 0.031	2.526LysVal: 2.526 ± 0.038	0.387LysTrp: 0.387 ± 0.012	0.66LysTyr: 0.66 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.546LeuAla: 14.546 ± 0.101	1.027LeuCys: 1.027 ± 0.022	5.738LeuAsp: 5.738 ± 0.054	5.24LeuGlu: 5.24 ± 0.052	3.402LeuPhe: 3.402 ± 0.048	8.792LeuGly: 8.792 ± 0.063	2.326LeuHis: 2.326 ± 0.034	4.355LeuIle: 4.355 ± 0.047	3.391LeuLys: 3.391 ± 0.04	10.489LeuLeu: 10.489 ± 0.104	2.49LeuMet: 2.49 ± 0.034	2.5LeuAsn: 2.5 ± 0.037	5.862LeuPro: 5.862 ± 0.055	3.703LeuGln: 3.703 ± 0.044	7.904LeuArg: 7.904 ± 0.071	6.01LeuSer: 6.01 ± 0.05	4.948LeuThr: 4.948 ± 0.042	7.973LeuVal: 7.973 ± 0.069	1.262LeuTrp: 1.262 ± 0.023	1.967LeuTyr: 1.967 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.962MetAla: 2.962 ± 0.036	0.188MetCys: 0.188 ± 0.01	1.071MetAsp: 1.071 ± 0.022	1.07MetGlu: 1.07 ± 0.022	0.731MetPhe: 0.731 ± 0.019	1.797MetGly: 1.797 ± 0.031	0.587MetHis: 0.587 ± 0.014	0.978MetIle: 0.978 ± 0.019	1.078MetLys: 1.078 ± 0.024	2.718MetLeu: 2.718 ± 0.035	0.564MetMet: 0.564 ± 0.017	0.912MetAsn: 0.912 ± 0.019	1.553MetPro: 1.553 ± 0.029	1.032MetGln: 1.032 ± 0.024	1.902MetArg: 1.902 ± 0.03	1.701MetSer: 1.701 ± 0.028	1.649MetThr: 1.649 ± 0.023	1.715MetVal: 1.715 ± 0.025	0.233MetTrp: 0.233 ± 0.01	0.384MetTyr: 0.384 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.302AsnAla: 3.302 ± 0.044	0.269AsnCys: 0.269 ± 0.012	1.257AsnAsp: 1.257 ± 0.024	1.245AsnGlu: 1.245 ± 0.024	0.932AsnPhe: 0.932 ± 0.022	2.253AsnGly: 2.253 ± 0.036	0.495AsnHis: 0.495 ± 0.016	1.183AsnIle: 1.183 ± 0.023	0.768AsnLys: 0.768 ± 0.02	2.55AsnLeu: 2.55 ± 0.036	0.575AsnMet: 0.575 ± 0.017	0.693AsnAsn: 0.693 ± 0.021	1.821AsnPro: 1.821 ± 0.031	0.811AsnGln: 0.811 ± 0.022	1.652AsnArg: 1.652 ± 0.026	1.237AsnSer: 1.237 ± 0.029	1.336AsnThr: 1.336 ± 0.025	1.945AsnVal: 1.945 ± 0.031	0.423AsnTrp: 0.423 ± 0.016	0.688AsnTyr: 0.688 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.1ProAla: 7.1 ± 0.076	0.378ProCys: 0.378 ± 0.014	3.199ProAsp: 3.199 ± 0.04	3.344ProGlu: 3.344 ± 0.046	1.859ProPhe: 1.859 ± 0.027	4.794ProGly: 4.794 ± 0.05	1.137ProHis: 1.137 ± 0.023	2.112ProIle: 2.112 ± 0.028	1.638ProLys: 1.638 ± 0.028	5.122ProLeu: 5.122 ± 0.044	1.413ProMet: 1.413 ± 0.024	1.322ProAsn: 1.322 ± 0.028	2.847ProPro: 2.847 ± 0.045	1.934ProGln: 1.934 ± 0.03	3.154ProArg: 3.154 ± 0.037	3.03ProSer: 3.03 ± 0.037	2.672ProThr: 2.672 ± 0.033	4.424ProVal: 4.424 ± 0.045	0.813ProTrp: 0.813 ± 0.02	1.241ProTyr: 1.241 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.791GlnAla: 4.791 ± 0.05	0.279GlnCys: 0.279 ± 0.011	1.488GlnAsp: 1.488 ± 0.025	1.482GlnGlu: 1.482 ± 0.025	1.212GlnPhe: 1.212 ± 0.028	2.78GlnGly: 2.78 ± 0.038	0.81GlnHis: 0.81 ± 0.02	1.73GlnIle: 1.73 ± 0.026	1.143GlnLys: 1.143 ± 0.024	3.796GlnLeu: 3.796 ± 0.041	0.93GlnMet: 0.93 ± 0.021	0.787GlnAsn: 0.787 ± 0.019	2.112GlnPro: 2.112 ± 0.032	1.733GlnGln: 1.733 ± 0.043	3.159GlnArg: 3.159 ± 0.043	1.94GlnSer: 1.94 ± 0.028	1.728GlnThr: 1.728 ± 0.03	2.802GlnVal: 2.802 ± 0.035	0.596GlnTrp: 0.596 ± 0.017	0.699GlnTyr: 0.699 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	8.424ArgAla: 8.424 ± 0.066	0.66ArgCys: 0.66 ± 0.02	3.717ArgAsp: 3.717 ± 0.043	4.472ArgGlu: 4.472 ± 0.054	2.912ArgPhe: 2.912 ± 0.038	4.912ArgGly: 4.912 ± 0.048	1.892ArgHis: 1.892 ± 0.03	3.74ArgIle: 3.74 ± 0.041	2.256ArgLys: 2.256 ± 0.032	7.815ArgLeu: 7.815 ± 0.071	2.018ArgMet: 2.018 ± 0.03	1.856ArgAsn: 1.856 ± 0.028	3.526ArgPro: 3.526 ± 0.044	2.768ArgGln: 2.768 ± 0.041	5.75ArgArg: 5.75 ± 0.06	4.019ArgSer: 4.019 ± 0.047	3.423ArgThr: 3.423 ± 0.041	5.254ArgVal: 5.254 ± 0.048	1.224ArgTrp: 1.224 ± 0.022	1.743ArgTyr: 1.743 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.68SerAla: 6.68 ± 0.059	0.474SerCys: 0.474 ± 0.016	2.725SerAsp: 2.725 ± 0.033	2.654SerGlu: 2.654 ± 0.036	2.201SerPhe: 2.201 ± 0.029	5.338SerGly: 5.338 ± 0.058	1.229SerHis: 1.229 ± 0.025	2.53SerIle: 2.53 ± 0.035	1.627SerLys: 1.627 ± 0.027	5.675SerLeu: 5.675 ± 0.054	1.445SerMet: 1.445 ± 0.025	1.456SerAsn: 1.456 ± 0.028	3.128SerPro: 3.128 ± 0.039	1.841SerGln: 1.841 ± 0.028	3.777SerArg: 3.777 ± 0.037	3.302SerSer: 3.302 ± 0.047	2.995SerThr: 2.995 ± 0.043	4.143SerVal: 4.143 ± 0.041	0.767SerTrp: 0.767 ± 0.021	1.329SerTyr: 1.329 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.875ThrAla: 5.875 ± 0.061	0.428ThrCys: 0.428 ± 0.014	2.359ThrAsp: 2.359 ± 0.033	2.379ThrGlu: 2.379 ± 0.029	1.741ThrPhe: 1.741 ± 0.027	4.738ThrGly: 4.738 ± 0.054	1.219ThrHis: 1.219 ± 0.02	2.205ThrIle: 2.205 ± 0.032	1.18ThrLys: 1.18 ± 0.025	5.916ThrLeu: 5.916 ± 0.057	1.18ThrMet: 1.18 ± 0.022	1.188ThrAsn: 1.188 ± 0.026	3.488ThrPro: 3.488 ± 0.035	1.783ThrGln: 1.783 ± 0.028	3.375ThrArg: 3.375 ± 0.034	2.81ThrSer: 2.81 ± 0.042	2.752ThrThr: 2.752 ± 0.045	4.183ThrVal: 4.183 ± 0.041	0.697ThrTrp: 0.697 ± 0.018	1.064ThrTyr: 1.064 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	9.832ValAla: 9.832 ± 0.066	0.747ValCys: 0.747 ± 0.02	4.164ValAsp: 4.164 ± 0.049	4.237ValGlu: 4.237 ± 0.042	2.781ValPhe: 2.781 ± 0.039	5.808ValGly: 5.808 ± 0.054	1.654ValHis: 1.654 ± 0.028	3.625ValIle: 3.625 ± 0.045	2.382ValLys: 2.382 ± 0.044	8.377ValLeu: 8.377 ± 0.078	1.962ValMet: 1.962 ± 0.03	2.115ValAsn: 2.115 ± 0.035	4.306ValPro: 4.306 ± 0.04	2.699ValGln: 2.699 ± 0.034	5.56ValArg: 5.56 ± 0.049	4.177ValSer: 4.177 ± 0.042	4.046ValThr: 4.046 ± 0.045	6.539ValVal: 6.539 ± 0.065	0.962ValTrp: 0.962 ± 0.021	1.532ValTyr: 1.532 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.298TrpAla: 1.298 ± 0.025	0.149TrpCys: 0.149 ± 0.008	0.639TrpAsp: 0.639 ± 0.018	0.565TrpGlu: 0.565 ± 0.018	0.58TrpPhe: 0.58 ± 0.017	1.023TrpGly: 1.023 ± 0.02	0.37TrpHis: 0.37 ± 0.012	0.713TrpIle: 0.713 ± 0.018	0.48TrpLys: 0.48 ± 0.017	1.953TrpLeu: 1.953 ± 0.036	0.431TrpMet: 0.431 ± 0.014	0.465TrpAsn: 0.465 ± 0.016	0.685TrpPro: 0.685 ± 0.02	0.662TrpGln: 0.662 ± 0.017	1.267TrpArg: 1.267 ± 0.024	0.846TrpSer: 0.846 ± 0.019	0.764TrpThr: 0.764 ± 0.019	1.008TrpVal: 1.008 ± 0.02	0.265TrpTrp: 0.265 ± 0.011	0.317TrpTyr: 0.317 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.564TyrAla: 2.564 ± 0.035	0.238TyrCys: 0.238 ± 0.01	1.203TyrAsp: 1.203 ± 0.024	1.184TyrGlu: 1.184 ± 0.023	0.941TyrPhe: 0.941 ± 0.02	2.059TyrGly: 2.059 ± 0.029	0.437TyrHis: 0.437 ± 0.013	0.791TyrIle: 0.791 ± 0.019	0.668TyrLys: 0.668 ± 0.017	2.322TyrLeu: 2.322 ± 0.034	0.435TyrMet: 0.435 ± 0.014	0.577TyrAsn: 0.577 ± 0.017	1.143TyrPro: 1.143 ± 0.022	0.732TyrGln: 0.732 ± 0.019	1.656TyrArg: 1.656 ± 0.026	1.14TyrSer: 1.14 ± 0.021	1.128TyrThr: 1.128 ± 0.026	1.692TyrVal: 1.692 ± 0.025	0.388TyrTrp: 0.388 ± 0.011	0.587TyrTyr: 0.587 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7062 proteins (2305172 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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