Actinokineospora auranticolor

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinokineospora

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6GTR1|A0A2S6GTR1_9PSEU Phosphotransferase family enzyme OS=Actinokineospora auranticolor OX=155976 GN=CLV40_105308 PE=4 SV=1
MM1 pKa = 7.61TEE3 pKa = 4.45PDD5 pKa = 3.67KK6 pKa = 11.26TWDD9 pKa = 3.74DD10 pKa = 3.32GHH12 pKa = 7.9YY13 pKa = 11.22GEE15 pKa = 6.08DD16 pKa = 4.08DD17 pKa = 3.96VIEE20 pKa = 4.26AADD23 pKa = 3.65GKK25 pKa = 11.17GYY27 pKa = 10.79LSVSDD32 pKa = 4.1YY33 pKa = 10.79THH35 pKa = 7.08GDD37 pKa = 3.24NGLEE41 pKa = 4.29PDD43 pKa = 3.57DD44 pKa = 5.17HH45 pKa = 7.64SWW47 pKa = 3.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6GZ32|A0A2S6GZ32_9PSEU Betaine-aldehyde dehydrogenase OS=Actinokineospora auranticolor OX=155976 GN=CLV40_102341 PE=3 SV=1
MM1 pKa = 7.66AWPARR6 pKa = 11.84PPRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84PAGRR15 pKa = 11.84RR16 pKa = 11.84SRR18 pKa = 11.84TPPRR22 pKa = 11.84RR23 pKa = 11.84PGAPTSRR30 pKa = 11.84TRR32 pKa = 11.84PPSASPATAWASPRR46 pKa = 11.84PAPPGARR53 pKa = 11.84APPLPAPPGRR63 pKa = 11.84PRR65 pKa = 11.84PHH67 pKa = 6.76RR68 pKa = 11.84AAPRR72 pKa = 11.84PPQARR77 pKa = 11.84QTPRR81 pKa = 11.84PHH83 pKa = 6.69VPTPQGPAHH92 pKa = 6.86HH93 pKa = 7.02AHH95 pKa = 5.84QACPTARR102 pKa = 11.84TLRR105 pKa = 11.84VRR107 pKa = 11.84GRR109 pKa = 11.84PRR111 pKa = 11.84VRR113 pKa = 11.84RR114 pKa = 11.84GPRR117 pKa = 11.84PRR119 pKa = 11.84PVTGALQPRR128 pKa = 11.84VQVRR132 pKa = 11.84LRR134 pKa = 11.84ARR136 pKa = 11.84PVRR139 pKa = 11.84PLLPRR144 pKa = 11.84HH145 pKa = 5.85RR146 pKa = 11.84RR147 pKa = 11.84PPRR150 pKa = 11.84RR151 pKa = 11.84PARR154 pKa = 11.84ARR156 pKa = 11.84QGPARR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84LAGRR168 pKa = 11.84ARR170 pKa = 11.84LRR172 pKa = 11.84ARR174 pKa = 11.84RR175 pKa = 11.84RR176 pKa = 11.84GRR178 pKa = 11.84PPGARR183 pKa = 11.84VGRR186 pKa = 11.84LPRR189 pKa = 11.84PGRR192 pKa = 11.84GSPATHH198 pKa = 6.34HH199 pKa = 7.03LSRR202 pKa = 11.84HH203 pKa = 4.92LVGSLRR209 pKa = 11.84RR210 pKa = 11.84DD211 pKa = 3.2LARR214 pKa = 11.84PGKK217 pKa = 7.8TAAPRR222 pKa = 11.84PRR224 pKa = 11.84DD225 pKa = 3.05RR226 pKa = 11.84ARR228 pKa = 11.84RR229 pKa = 11.84EE230 pKa = 3.63RR231 pKa = 11.84VRR233 pKa = 11.84PHH235 pKa = 5.83PSKK238 pKa = 10.19RR239 pKa = 11.84TAHH242 pKa = 7.2RR243 pKa = 11.84SRR245 pKa = 11.84VCTGSSARR253 pKa = 11.84RR254 pKa = 11.84TRR256 pKa = 11.84HH257 pKa = 5.87RR258 pKa = 11.84GRR260 pKa = 11.84IDD262 pKa = 3.22RR263 pKa = 11.84VRR265 pKa = 11.84GKK267 pKa = 9.45VRR269 pKa = 11.84RR270 pKa = 11.84SRR272 pKa = 11.84TAPGPTRR279 pKa = 11.84DD280 pKa = 3.67TAHH283 pKa = 6.7KK284 pKa = 8.51VHH286 pKa = 7.3RR287 pKa = 11.84SRR289 pKa = 11.84ASRR292 pKa = 11.84SGVGTSRR299 pKa = 11.84IRR301 pKa = 11.84ATVRR305 pKa = 11.84GRR307 pKa = 11.84RR308 pKa = 11.84QAHH311 pKa = 6.32RR312 pKa = 11.84PSQATAGHH320 pKa = 7.03RR321 pKa = 11.84ANGHH325 pKa = 5.58RR326 pKa = 11.84PCPSHH331 pKa = 5.79MALRR335 pKa = 11.84PGLVTLRR342 pKa = 11.84RR343 pKa = 11.84RR344 pKa = 11.84ANAPRR349 pKa = 11.84VRR351 pKa = 11.84RR352 pKa = 11.84PAARR356 pKa = 11.84LSRR359 pKa = 11.84TALRR363 pKa = 11.84PKK365 pKa = 10.64GLTLRR370 pKa = 11.84RR371 pKa = 11.84NAPRR375 pKa = 11.84ARR377 pKa = 11.84RR378 pKa = 11.84TAPHH382 pKa = 7.18PEE384 pKa = 3.49RR385 pKa = 11.84ATRR388 pKa = 11.84RR389 pKa = 11.84RR390 pKa = 11.84RR391 pKa = 11.84AMVSPRR397 pKa = 11.84RR398 pKa = 11.84VIARR402 pKa = 11.84SRR404 pKa = 11.84ALDD407 pKa = 3.38RR408 pKa = 11.84PTRR411 pKa = 11.84GRR413 pKa = 11.84RR414 pKa = 11.84PPPVRR419 pKa = 11.84HH420 pKa = 6.41DD421 pKa = 3.5RR422 pKa = 11.84APVRR426 pKa = 11.84TGNRR430 pKa = 11.84RR431 pKa = 11.84PDD433 pKa = 3.23RR434 pKa = 11.84PRR436 pKa = 11.84GLRR439 pKa = 11.84MATVPTGTTRR449 pKa = 11.84RR450 pKa = 11.84RR451 pKa = 11.84GASRR455 pKa = 11.84QRR457 pKa = 11.84RR458 pKa = 11.84TRR460 pKa = 11.84GPNHH464 pKa = 5.9VVRR467 pKa = 11.84TTPGARR473 pKa = 11.84SRR475 pKa = 11.84RR476 pKa = 11.84EE477 pKa = 3.75TSATAPTRR485 pKa = 11.84TPPANAPATTRR496 pKa = 11.84AGARR500 pKa = 11.84TRR502 pKa = 11.84TQPTTGPRR510 pKa = 11.84TRR512 pKa = 11.84TRR514 pKa = 11.84PRR516 pKa = 11.84ITGGTRR522 pKa = 11.84TGPSRR527 pKa = 11.84TGRR530 pKa = 11.84PTRR533 pKa = 11.84GSRR536 pKa = 11.84PTSAGTPRR544 pKa = 11.84TSRR547 pKa = 11.84TSTTPPTPSCWCARR561 pKa = 11.84STARR565 pKa = 11.84RR566 pKa = 11.84RR567 pKa = 11.84AAA569 pKa = 3.47

Molecular weight: 63.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7400

0

7400

2468596

26

7590

333.6

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.79 ± 0.045

0.777 ± 0.009

6.312 ± 0.026

5.196 ± 0.03

2.714 ± 0.014

9.158 ± 0.03

2.277 ± 0.015

3.051 ± 0.018

1.8 ± 0.024

10.406 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.483 ± 0.012

1.816 ± 0.021

6.227 ± 0.026

2.492 ± 0.017

8.305 ± 0.04

4.901 ± 0.026

6.488 ± 0.042

9.406 ± 0.033

1.524 ± 0.012

1.875 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski