Amino acid dipepetide frequency for Granulicatella sp. WM01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.794AlaAla: 3.794 ± 0.167
0.649AlaCys: 0.649 ± 0.041
2.882AlaAsp: 2.882 ± 0.084
3.457AlaGlu: 3.457 ± 0.101
2.693AlaPhe: 2.693 ± 0.08
3.925AlaGly: 3.925 ± 0.103
1.465AlaHis: 1.465 ± 0.056
6.111AlaIle: 6.111 ± 0.144
5.416AlaLys: 5.416 ± 0.223
7.159AlaLeu: 7.159 ± 0.142
1.76AlaMet: 1.76 ± 0.058
3.06AlaAsn: 3.06 ± 0.083
1.732AlaPro: 1.732 ± 0.053
2.905AlaGln: 2.905 ± 0.075
2.547AlaArg: 2.547 ± 0.07
4.105AlaSer: 4.105 ± 0.267
3.929AlaThr: 3.929 ± 0.1
4.078AlaVal: 4.078 ± 0.1
0.515AlaTrp: 0.515 ± 0.033
2.655AlaTyr: 2.655 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.522CysAla: 0.522 ± 0.032
0.129CysCys: 0.129 ± 0.017
0.377CysAsp: 0.377 ± 0.027
0.486CysGlu: 0.486 ± 0.035
0.352CysPhe: 0.352 ± 0.023
0.609CysGly: 0.609 ± 0.033
0.318CysHis: 0.318 ± 0.026
0.698CysIle: 0.698 ± 0.037
0.343CysLys: 0.343 ± 0.027
0.924CysLeu: 0.924 ± 0.043
0.204CysMet: 0.204 ± 0.021
0.276CysAsn: 0.276 ± 0.023
0.261CysPro: 0.261 ± 0.028
0.528CysGln: 0.528 ± 0.034
0.274CysArg: 0.274 ± 0.025
0.481CysSer: 0.481 ± 0.035
0.358CysThr: 0.358 ± 0.026
0.693CysVal: 0.693 ± 0.044
0.053CysTrp: 0.053 ± 0.009
0.371CysTyr: 0.371 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
3.533AspAla: 3.533 ± 0.131
0.407AspCys: 0.407 ± 0.029
2.759AspAsp: 2.759 ± 0.086
4.207AspGlu: 4.207 ± 0.106
2.392AspPhe: 2.392 ± 0.07
3.077AspGly: 3.077 ± 0.089
0.691AspHis: 0.691 ± 0.037
4.981AspIle: 4.981 ± 0.087
4.126AspLys: 4.126 ± 0.137
4.479AspLeu: 4.479 ± 0.094
1.546AspMet: 1.546 ± 0.064
2.776AspAsn: 2.776 ± 0.091
1.19AspPro: 1.19 ± 0.05
1.052AspGln: 1.052 ± 0.046
1.716AspArg: 1.716 ± 0.063
2.852AspSer: 2.852 ± 0.065
3.804AspThr: 3.804 ± 0.098
4.419AspVal: 4.419 ± 0.097
0.507AspTrp: 0.507 ± 0.035
2.579AspTyr: 2.579 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
4.267GluAla: 4.267 ± 0.104
0.534GluCys: 0.534 ± 0.031
3.522GluAsp: 3.522 ± 0.092
4.956GluGlu: 4.956 ± 0.135
2.538GluPhe: 2.538 ± 0.065
2.858GluGly: 2.858 ± 0.077
2.267GluHis: 2.267 ± 0.079
4.754GluIle: 4.754 ± 0.117
6.194GluLys: 6.194 ± 0.134
7.057GluLeu: 7.057 ± 0.125
1.866GluMet: 1.866 ± 0.062
3.77GluAsn: 3.77 ± 0.089
1.522GluPro: 1.522 ± 0.057
4.046GluGln: 4.046 ± 0.134
3.183GluArg: 3.183 ± 0.091
3.512GluSer: 3.512 ± 0.2
3.792GluThr: 3.792 ± 0.122
4.188GluVal: 4.188 ± 0.094
0.785GluTrp: 0.785 ± 0.041
2.693GluTyr: 2.693 ± 0.082
0.0GluXaa: 0.0 ± 0.0
Phe
2.49PheAla: 2.49 ± 0.074
0.341PheCys: 0.341 ± 0.026
2.598PheAsp: 2.598 ± 0.087
2.719PheGlu: 2.719 ± 0.066
1.752PhePhe: 1.752 ± 0.06
2.907PheGly: 2.907 ± 0.073
0.793PheHis: 0.793 ± 0.044
3.624PheIle: 3.624 ± 0.108
2.714PheLys: 2.714 ± 0.077
3.753PheLeu: 3.753 ± 0.096
1.107PheMet: 1.107 ± 0.046
2.076PheAsn: 2.076 ± 0.061
1.372PhePro: 1.372 ± 0.048
1.336PheGln: 1.336 ± 0.05
1.336PheArg: 1.336 ± 0.054
3.34PheSer: 3.34 ± 0.094
2.318PheThr: 2.318 ± 0.068
2.877PheVal: 2.877 ± 0.081
0.325PheTrp: 0.325 ± 0.026
1.603PheTyr: 1.603 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
3.925GlyAla: 3.925 ± 0.116
0.473GlyCys: 0.473 ± 0.032
2.899GlyAsp: 2.899 ± 0.139
3.925GlyGlu: 3.925 ± 0.096
2.576GlyPhe: 2.576 ± 0.081
3.726GlyGly: 3.726 ± 0.106
1.289GlyHis: 1.289 ± 0.05
5.35GlyIle: 5.35 ± 0.103
4.824GlyLys: 4.824 ± 0.114
5.505GlyLeu: 5.505 ± 0.128
1.709GlyMet: 1.709 ± 0.061
2.723GlyAsn: 2.723 ± 0.082
0.976GlyPro: 0.976 ± 0.048
2.56GlyGln: 2.56 ± 0.077
2.263GlyArg: 2.263 ± 0.079
3.291GlySer: 3.291 ± 0.089
3.828GlyThr: 3.828 ± 0.103
4.752GlyVal: 4.752 ± 0.118
0.462GlyTrp: 0.462 ± 0.032
2.829GlyTyr: 2.829 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
1.641HisAla: 1.641 ± 0.066
0.199HisCys: 0.199 ± 0.021
1.276HisAsp: 1.276 ± 0.056
1.463HisGlu: 1.463 ± 0.054
1.183HisPhe: 1.183 ± 0.046
1.344HisGly: 1.344 ± 0.052
0.969HisHis: 0.969 ± 0.048
2.473HisIle: 2.473 ± 0.084
1.294HisLys: 1.294 ± 0.047
2.648HisLeu: 2.648 ± 0.078
0.426HisMet: 0.426 ± 0.026
0.941HisAsn: 0.941 ± 0.044
0.872HisPro: 0.872 ± 0.037
1.177HisGln: 1.177 ± 0.051
0.836HisArg: 0.836 ± 0.041
1.255HisSer: 1.255 ± 0.046
1.423HisThr: 1.423 ± 0.057
2.027HisVal: 2.027 ± 0.07
0.15HisTrp: 0.15 ± 0.017
1.304HisTyr: 1.304 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
5.857IleAla: 5.857 ± 0.127
0.74IleCys: 0.74 ± 0.039
5.064IleAsp: 5.064 ± 0.129
5.738IleGlu: 5.738 ± 0.115
3.221IlePhe: 3.221 ± 0.085
5.297IleGly: 5.297 ± 0.107
1.919IleHis: 1.919 ± 0.068
6.103IleIle: 6.103 ± 0.126
5.111IleLys: 5.111 ± 0.102
7.522IleLeu: 7.522 ± 0.136
1.786IleMet: 1.786 ± 0.059
3.658IleAsn: 3.658 ± 0.087
2.884IlePro: 2.884 ± 0.074
3.48IleGln: 3.48 ± 0.094
2.965IleArg: 2.965 ± 0.077
5.255IleSer: 5.255 ± 0.104
4.326IleThr: 4.326 ± 0.094
6.158IleVal: 6.158 ± 0.129
0.492IleTrp: 0.492 ± 0.029
2.791IleTyr: 2.791 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
4.468LysAla: 4.468 ± 0.164
0.392LysCys: 0.392 ± 0.027
4.056LysAsp: 4.056 ± 0.105
6.353LysGlu: 6.353 ± 0.118
1.847LysPhe: 1.847 ± 0.055
4.088LysGly: 4.088 ± 0.142
2.002LysHis: 2.002 ± 0.062
4.782LysIle: 4.782 ± 0.11
5.596LysLys: 5.596 ± 0.131
5.611LysLeu: 5.611 ± 0.108
2.057LysMet: 2.057 ± 0.062
3.463LysAsn: 3.463 ± 0.098
2.05LysPro: 2.05 ± 0.108
4.394LysGln: 4.394 ± 0.103
3.465LysArg: 3.465 ± 0.087
3.579LysSer: 3.579 ± 0.094
4.46LysThr: 4.46 ± 0.117
4.631LysVal: 4.631 ± 0.084
0.681LysTrp: 0.681 ± 0.038
2.598LysTyr: 2.598 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
6.807LeuAla: 6.807 ± 0.115
0.948LeuCys: 0.948 ± 0.044
5.469LeuAsp: 5.469 ± 0.12
6.173LeuGlu: 6.173 ± 0.134
4.269LeuPhe: 4.269 ± 0.113
6.009LeuGly: 6.009 ± 0.128
2.148LeuHis: 2.148 ± 0.067
6.807LeuIle: 6.807 ± 0.136
6.669LeuLys: 6.669 ± 0.121
9.362LeuLeu: 9.362 ± 0.16
2.434LeuMet: 2.434 ± 0.074
4.85LeuAsn: 4.85 ± 0.086
3.635LeuPro: 3.635 ± 0.076
3.664LeuGln: 3.664 ± 0.095
3.376LeuArg: 3.376 ± 0.085
8.421LeuSer: 8.421 ± 0.53
6.041LeuThr: 6.041 ± 0.118
6.4LeuVal: 6.4 ± 0.109
0.677LeuTrp: 0.677 ± 0.039
3.342LeuTyr: 3.342 ± 0.096
0.0LeuXaa: 0.0 ± 0.0
Met
1.788MetAla: 1.788 ± 0.073
0.189MetCys: 0.189 ± 0.018
1.24MetAsp: 1.24 ± 0.046
1.402MetGlu: 1.402 ± 0.058
0.908MetPhe: 0.908 ± 0.047
1.438MetGly: 1.438 ± 0.061
0.524MetHis: 0.524 ± 0.027
2.059MetIle: 2.059 ± 0.068
1.93MetLys: 1.93 ± 0.058
2.432MetLeu: 2.432 ± 0.079
0.751MetMet: 0.751 ± 0.04
1.353MetAsn: 1.353 ± 0.054
0.937MetPro: 0.937 ± 0.041
1.024MetGln: 1.024 ± 0.045
0.967MetArg: 0.967 ± 0.042
1.962MetSer: 1.962 ± 0.074
1.813MetThr: 1.813 ± 0.058
1.59MetVal: 1.59 ± 0.053
0.148MetTrp: 0.148 ± 0.017
0.876MetTyr: 0.876 ± 0.043
0.002MetXaa: 0.002 ± 0.002
Asn
3.143AsnAla: 3.143 ± 0.114
0.327AsnCys: 0.327 ± 0.026
2.303AsnAsp: 2.303 ± 0.077
3.049AsnGlu: 3.049 ± 0.086
1.51AsnPhe: 1.51 ± 0.058
3.499AsnGly: 3.499 ± 0.1
1.317AsnHis: 1.317 ± 0.055
4.014AsnIle: 4.014 ± 0.099
3.094AsnLys: 3.094 ± 0.094
3.73AsnLeu: 3.73 ± 0.104
1.234AsnMet: 1.234 ± 0.045
2.21AsnAsn: 2.21 ± 0.082
2.159AsnPro: 2.159 ± 0.077
2.506AsnGln: 2.506 ± 0.086
2.112AsnArg: 2.112 ± 0.068
2.384AsnSer: 2.384 ± 0.077
2.691AsnThr: 2.691 ± 0.087
3.278AsnVal: 3.278 ± 0.087
0.422AsnTrp: 0.422 ± 0.027
1.847AsnTyr: 1.847 ± 0.063
0.0AsnXaa: 0.0 ± 0.0
Pro
1.671ProAla: 1.671 ± 0.065
0.182ProCys: 0.182 ± 0.02
1.609ProAsp: 1.609 ± 0.057
2.566ProGlu: 2.566 ± 0.14
1.635ProPhe: 1.635 ± 0.053
1.476ProGly: 1.476 ± 0.093
0.73ProHis: 0.73 ± 0.04
2.672ProIle: 2.672 ± 0.077
2.229ProLys: 2.229 ± 0.068
2.854ProLeu: 2.854 ± 0.078
0.708ProMet: 0.708 ± 0.035
1.718ProAsn: 1.718 ± 0.055
0.517ProPro: 0.517 ± 0.036
1.243ProGln: 1.243 ± 0.05
0.956ProArg: 0.956 ± 0.045
1.995ProSer: 1.995 ± 0.065
2.023ProThr: 2.023 ± 0.067
2.313ProVal: 2.313 ± 0.095
0.263ProTrp: 0.263 ± 0.022
1.395ProTyr: 1.395 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
3.456GlnAla: 3.456 ± 0.091
0.29GlnCys: 0.29 ± 0.022
2.392GlnAsp: 2.392 ± 0.076
3.815GlnGlu: 3.815 ± 0.096
2.019GlnPhe: 2.019 ± 0.073
2.367GlnGly: 2.367 ± 0.072
1.317GlnHis: 1.317 ± 0.061
2.907GlnIle: 2.907 ± 0.081
3.45GlnLys: 3.45 ± 0.089
5.0GlnLeu: 5.0 ± 0.132
1.086GlnMet: 1.086 ± 0.054
1.983GlnAsn: 1.983 ± 0.07
1.092GlnPro: 1.092 ± 0.085
2.526GlnGln: 2.526 ± 0.082
1.824GlnArg: 1.824 ± 0.059
2.894GlnSer: 2.894 ± 0.127
2.825GlnThr: 2.825 ± 0.082
2.998GlnVal: 2.998 ± 0.077
0.384GlnTrp: 0.384 ± 0.029
1.813GlnTyr: 1.813 ± 0.056
0.0GlnXaa: 0.0 ± 0.0
Arg
2.305ArgAla: 2.305 ± 0.065
0.327ArgCys: 0.327 ± 0.025
1.783ArgAsp: 1.783 ± 0.064
2.782ArgGlu: 2.782 ± 0.081
1.715ArgPhe: 1.715 ± 0.053
2.065ArgGly: 2.065 ± 0.067
1.052ArgHis: 1.052 ± 0.04
3.172ArgIle: 3.172 ± 0.087
2.648ArgLys: 2.648 ± 0.071
4.23ArgLeu: 4.23 ± 0.11
1.092ArgMet: 1.092 ± 0.043
1.397ArgAsn: 1.397 ± 0.052
1.039ArgPro: 1.039 ± 0.048
2.337ArgGln: 2.337 ± 0.071
1.769ArgArg: 1.769 ± 0.07
1.879ArgSer: 1.879 ± 0.058
1.798ArgThr: 1.798 ± 0.056
2.882ArgVal: 2.882 ± 0.069
0.308ArgTrp: 0.308 ± 0.026
1.862ArgTyr: 1.862 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
4.12SerAla: 4.12 ± 0.267
0.449SerCys: 0.449 ± 0.028
3.253SerAsp: 3.253 ± 0.087
4.126SerGlu: 4.126 ± 0.194
2.765SerPhe: 2.765 ± 0.08
4.065SerGly: 4.065 ± 0.099
1.506SerHis: 1.506 ± 0.058
5.248SerIle: 5.248 ± 0.107
3.813SerLys: 3.813 ± 0.093
6.603SerLeu: 6.603 ± 0.523
1.569SerMet: 1.569 ± 0.076
2.619SerAsn: 2.619 ± 0.092
1.824SerPro: 1.824 ± 0.062
3.255SerGln: 3.255 ± 0.148
2.182SerArg: 2.182 ± 0.061
4.054SerSer: 4.054 ± 0.126
4.101SerThr: 4.101 ± 0.336
4.937SerVal: 4.937 ± 0.122
0.483SerTrp: 0.483 ± 0.028
2.335SerTyr: 2.335 ± 0.072
0.0SerXaa: 0.0 ± 0.0
Thr
3.242ThrAla: 3.242 ± 0.105
0.522ThrCys: 0.522 ± 0.033
2.877ThrAsp: 2.877 ± 0.089
3.168ThrGlu: 3.168 ± 0.086
2.666ThrPhe: 2.666 ± 0.079
3.897ThrGly: 3.897 ± 0.144
1.648ThrHis: 1.648 ± 0.056
5.2ThrIle: 5.2 ± 0.102
3.88ThrLys: 3.88 ± 0.088
6.858ThrLeu: 6.858 ± 0.144
1.196ThrMet: 1.196 ± 0.047
2.547ThrAsn: 2.547 ± 0.083
2.574ThrPro: 2.574 ± 0.081
2.964ThrGln: 2.964 ± 0.104
2.059ThrArg: 2.059 ± 0.06
3.895ThrSer: 3.895 ± 0.34
3.775ThrThr: 3.775 ± 0.131
4.406ThrVal: 4.406 ± 0.112
0.433ThrTrp: 0.433 ± 0.03
2.543ThrTyr: 2.543 ± 0.074
0.0ThrXaa: 0.0 ± 0.0
Val
4.657ValAla: 4.657 ± 0.117
0.708ValCys: 0.708 ± 0.04
4.141ValAsp: 4.141 ± 0.107
4.96ValGlu: 4.96 ± 0.116
2.907ValPhe: 2.907 ± 0.091
4.347ValGly: 4.347 ± 0.093
1.495ValHis: 1.495 ± 0.054
5.558ValIle: 5.558 ± 0.099
4.404ValLys: 4.404 ± 0.107
6.987ValLeu: 6.987 ± 0.141
1.773ValMet: 1.773 ± 0.066
3.274ValAsn: 3.274 ± 0.074
2.595ValPro: 2.595 ± 0.086
2.772ValGln: 2.772 ± 0.078
2.5ValArg: 2.5 ± 0.08
5.306ValSer: 5.306 ± 0.13
4.078ValThr: 4.078 ± 0.118
5.316ValVal: 5.316 ± 0.116
0.503ValTrp: 0.503 ± 0.035
2.88ValTyr: 2.88 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.416TrpAla: 0.416 ± 0.027
0.074TrpCys: 0.074 ± 0.013
0.405TrpAsp: 0.405 ± 0.029
0.606TrpGlu: 0.606 ± 0.031
0.414TrpPhe: 0.414 ± 0.025
0.462TrpGly: 0.462 ± 0.031
0.223TrpHis: 0.223 ± 0.018
0.763TrpIle: 0.763 ± 0.043
0.581TrpLys: 0.581 ± 0.033
0.829TrpLeu: 0.829 ± 0.042
0.142TrpMet: 0.142 ± 0.017
0.426TrpAsn: 0.426 ± 0.028
0.114TrpPro: 0.114 ± 0.016
0.433TrpGln: 0.433 ± 0.029
0.308TrpArg: 0.308 ± 0.028
0.549TrpSer: 0.549 ± 0.036
0.388TrpThr: 0.388 ± 0.029
0.454TrpVal: 0.454 ± 0.029
0.068TrpTrp: 0.068 ± 0.01
0.324TrpTyr: 0.324 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.733TyrAla: 2.733 ± 0.069
0.377TyrCys: 0.377 ± 0.027
2.191TyrAsp: 2.191 ± 0.075
2.352TyrGlu: 2.352 ± 0.069
2.019TyrPhe: 2.019 ± 0.059
2.434TyrGly: 2.434 ± 0.067
1.224TyrHis: 1.224 ± 0.046
3.094TyrIle: 3.094 ± 0.086
2.258TyrLys: 2.258 ± 0.076
3.961TyrLeu: 3.961 ± 0.098
0.888TyrMet: 0.888 ± 0.043
1.709TyrAsn: 1.709 ± 0.058
1.41TyrPro: 1.41 ± 0.052
2.235TyrGln: 2.235 ± 0.082
1.8TyrArg: 1.8 ± 0.062
2.297TyrSer: 2.297 ± 0.079
2.576TyrThr: 2.576 ± 0.081
2.767TyrVal: 2.767 ± 0.091
0.318TyrTrp: 0.318 ± 0.03
1.658TyrTyr: 1.658 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.002XaaCys: 0.002 ± 0.002
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1694 proteins (528419 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski