Amino acid dipepetide frequency for Pseudomonas phage DL54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.138AlaAla: 8.138 ± 1.54
0.857AlaCys: 0.857 ± 0.281
4.14AlaAsp: 4.14 ± 0.534
6.353AlaGlu: 6.353 ± 0.851
2.855AlaPhe: 2.855 ± 0.42
6.71AlaGly: 6.71 ± 1.202
1.214AlaHis: 1.214 ± 0.317
4.925AlaIle: 4.925 ± 0.691
4.997AlaLys: 4.997 ± 0.686
7.281AlaLeu: 7.281 ± 0.751
2.57AlaMet: 2.57 ± 0.496
3.783AlaAsn: 3.783 ± 0.606
3.212AlaPro: 3.212 ± 0.609
5.282AlaGln: 5.282 ± 1.072
3.284AlaArg: 3.284 ± 0.56
5.14AlaSer: 5.14 ± 0.627
3.355AlaThr: 3.355 ± 0.585
5.925AlaVal: 5.925 ± 0.93
0.857AlaTrp: 0.857 ± 0.256
2.784AlaTyr: 2.784 ± 0.511
0.0AlaXaa: 0.0 ± 0.0
Cys
0.928CysAla: 0.928 ± 0.298
0.143CysCys: 0.143 ± 0.101
0.785CysAsp: 0.785 ± 0.275
1.142CysGlu: 1.142 ± 0.27
0.214CysPhe: 0.214 ± 0.118
0.928CysGly: 0.928 ± 0.292
0.5CysHis: 0.5 ± 0.185
0.642CysIle: 0.642 ± 0.228
0.357CysLys: 0.357 ± 0.132
0.571CysLeu: 0.571 ± 0.187
0.143CysMet: 0.143 ± 0.09
0.5CysAsn: 0.5 ± 0.207
0.357CysPro: 0.357 ± 0.151
0.357CysGln: 0.357 ± 0.157
0.286CysArg: 0.286 ± 0.126
0.714CysSer: 0.714 ± 0.218
0.5CysThr: 0.5 ± 0.186
0.571CysVal: 0.571 ± 0.272
0.357CysTrp: 0.357 ± 0.177
0.357CysTyr: 0.357 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
4.997AspAla: 4.997 ± 0.59
0.286AspCys: 0.286 ± 0.128
2.998AspAsp: 2.998 ± 0.48
3.997AspGlu: 3.997 ± 0.667
2.07AspPhe: 2.07 ± 0.436
5.282AspGly: 5.282 ± 0.687
1.57AspHis: 1.57 ± 0.404
3.498AspIle: 3.498 ± 0.419
3.212AspLys: 3.212 ± 0.504
6.068AspLeu: 6.068 ± 0.698
1.499AspMet: 1.499 ± 0.302
2.213AspAsn: 2.213 ± 0.311
3.641AspPro: 3.641 ± 0.576
1.499AspGln: 1.499 ± 0.399
3.641AspArg: 3.641 ± 0.56
2.855AspSer: 2.855 ± 0.474
3.712AspThr: 3.712 ± 0.578
2.998AspVal: 2.998 ± 0.395
1.785AspTrp: 1.785 ± 0.342
1.785AspTyr: 1.785 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
6.567GluAla: 6.567 ± 1.044
0.785GluCys: 0.785 ± 0.241
4.14GluAsp: 4.14 ± 0.575
6.781GluGlu: 6.781 ± 1.06
2.498GluPhe: 2.498 ± 0.439
5.14GluGly: 5.14 ± 0.616
1.142GluHis: 1.142 ± 0.274
3.783GluIle: 3.783 ± 0.446
3.926GluLys: 3.926 ± 0.602
6.639GluLeu: 6.639 ± 0.776
2.498GluMet: 2.498 ± 0.482
2.427GluAsn: 2.427 ± 0.41
1.999GluPro: 1.999 ± 0.376
2.284GluGln: 2.284 ± 0.46
5.354GluArg: 5.354 ± 0.677
3.212GluSer: 3.212 ± 0.592
2.784GluThr: 2.784 ± 0.538
6.924GluVal: 6.924 ± 0.734
0.857GluTrp: 0.857 ± 0.255
1.999GluTyr: 1.999 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
1.856PheAla: 1.856 ± 0.464
0.357PheCys: 0.357 ± 0.154
2.141PheAsp: 2.141 ± 0.407
1.927PheGlu: 1.927 ± 0.407
1.285PhePhe: 1.285 ± 0.415
4.212PheGly: 4.212 ± 0.467
0.642PheHis: 0.642 ± 0.195
2.713PheIle: 2.713 ± 0.569
1.785PheLys: 1.785 ± 0.312
3.926PheLeu: 3.926 ± 0.512
0.999PheMet: 0.999 ± 0.215
1.642PheAsn: 1.642 ± 0.331
1.785PhePro: 1.785 ± 0.38
1.57PheGln: 1.57 ± 0.349
1.642PheArg: 1.642 ± 0.37
2.57PheSer: 2.57 ± 0.432
2.141PheThr: 2.141 ± 0.325
2.427PheVal: 2.427 ± 0.407
0.857PheTrp: 0.857 ± 0.276
1.071PheTyr: 1.071 ± 0.296
0.0PheXaa: 0.0 ± 0.0
Gly
6.996GlyAla: 6.996 ± 1.108
0.928GlyCys: 0.928 ± 0.261
5.282GlyAsp: 5.282 ± 0.581
4.711GlyGlu: 4.711 ± 0.679
2.998GlyPhe: 2.998 ± 0.496
6.924GlyGly: 6.924 ± 1.085
1.713GlyHis: 1.713 ± 0.397
4.426GlyIle: 4.426 ± 0.501
5.282GlyLys: 5.282 ± 0.64
5.568GlyLeu: 5.568 ± 0.66
2.356GlyMet: 2.356 ± 0.411
3.855GlyAsn: 3.855 ± 0.678
2.927GlyPro: 2.927 ± 0.488
3.069GlyGln: 3.069 ± 0.345
4.283GlyArg: 4.283 ± 0.551
5.639GlySer: 5.639 ± 0.736
5.282GlyThr: 5.282 ± 0.654
6.424GlyVal: 6.424 ± 0.627
1.214GlyTrp: 1.214 ± 0.35
2.57GlyTyr: 2.57 ± 0.403
0.0GlyXaa: 0.0 ± 0.0
His
1.356HisAla: 1.356 ± 0.365
0.286HisCys: 0.286 ± 0.115
0.714HisAsp: 0.714 ± 0.213
1.356HisGlu: 1.356 ± 0.434
1.142HisPhe: 1.142 ± 0.267
1.642HisGly: 1.642 ± 0.383
0.428HisHis: 0.428 ± 0.194
1.356HisIle: 1.356 ± 0.273
1.071HisLys: 1.071 ± 0.275
2.784HisLeu: 2.784 ± 0.553
0.428HisMet: 0.428 ± 0.197
0.642HisAsn: 0.642 ± 0.216
0.571HisPro: 0.571 ± 0.204
0.571HisGln: 0.571 ± 0.193
1.57HisArg: 1.57 ± 0.391
0.928HisSer: 0.928 ± 0.291
0.714HisThr: 0.714 ± 0.199
1.071HisVal: 1.071 ± 0.264
0.571HisTrp: 0.571 ± 0.246
0.857HisTyr: 0.857 ± 0.27
0.0HisXaa: 0.0 ± 0.0
Ile
4.283IleAla: 4.283 ± 0.514
0.571IleCys: 0.571 ± 0.201
2.641IleAsp: 2.641 ± 0.484
3.141IleGlu: 3.141 ± 0.454
2.356IlePhe: 2.356 ± 0.48
4.925IleGly: 4.925 ± 0.452
0.928IleHis: 0.928 ± 0.309
2.784IleIle: 2.784 ± 0.45
3.712IleLys: 3.712 ± 0.513
3.997IleLeu: 3.997 ± 0.509
1.214IleMet: 1.214 ± 0.304
2.141IleAsn: 2.141 ± 0.371
3.284IlePro: 3.284 ± 0.607
2.57IleGln: 2.57 ± 0.47
3.641IleArg: 3.641 ± 0.499
2.855IleSer: 2.855 ± 0.461
2.927IleThr: 2.927 ± 0.482
2.784IleVal: 2.784 ± 0.447
0.714IleTrp: 0.714 ± 0.206
2.141IleTyr: 2.141 ± 0.339
0.0IleXaa: 0.0 ± 0.0
Lys
4.925LysAla: 4.925 ± 0.833
0.357LysCys: 0.357 ± 0.168
3.997LysAsp: 3.997 ± 0.453
4.14LysGlu: 4.14 ± 0.411
2.07LysPhe: 2.07 ± 0.416
3.997LysGly: 3.997 ± 0.597
1.428LysHis: 1.428 ± 0.322
2.998LysIle: 2.998 ± 0.407
3.569LysLys: 3.569 ± 0.483
4.854LysLeu: 4.854 ± 0.662
1.285LysMet: 1.285 ± 0.298
2.213LysAsn: 2.213 ± 0.437
2.356LysPro: 2.356 ± 0.404
1.57LysGln: 1.57 ± 0.359
3.712LysArg: 3.712 ± 0.558
3.069LysSer: 3.069 ± 0.416
2.784LysThr: 2.784 ± 0.501
5.068LysVal: 5.068 ± 0.648
1.356LysTrp: 1.356 ± 0.387
2.641LysTyr: 2.641 ± 0.31
0.0LysXaa: 0.0 ± 0.0
Leu
7.138LeuAla: 7.138 ± 0.669
0.928LeuCys: 0.928 ± 0.227
6.068LeuAsp: 6.068 ± 0.69
6.496LeuGlu: 6.496 ± 0.742
2.713LeuPhe: 2.713 ± 0.474
6.924LeuGly: 6.924 ± 0.851
1.142LeuHis: 1.142 ± 0.324
3.284LeuIle: 3.284 ± 0.497
5.925LeuLys: 5.925 ± 0.558
6.853LeuLeu: 6.853 ± 0.752
1.999LeuMet: 1.999 ± 0.509
3.641LeuAsn: 3.641 ± 0.499
3.212LeuPro: 3.212 ± 0.57
3.498LeuGln: 3.498 ± 0.611
6.068LeuArg: 6.068 ± 0.709
4.354LeuSer: 4.354 ± 0.726
3.426LeuThr: 3.426 ± 0.483
4.711LeuVal: 4.711 ± 0.521
0.857LeuTrp: 0.857 ± 0.303
2.141LeuTyr: 2.141 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
3.355MetAla: 3.355 ± 0.571
0.214MetCys: 0.214 ± 0.113
1.713MetAsp: 1.713 ± 0.338
1.285MetGlu: 1.285 ± 0.231
1.071MetPhe: 1.071 ± 0.262
1.856MetGly: 1.856 ± 0.423
0.286MetHis: 0.286 ± 0.103
1.713MetIle: 1.713 ± 0.412
1.856MetLys: 1.856 ± 0.345
1.642MetLeu: 1.642 ± 0.302
0.571MetMet: 0.571 ± 0.19
1.142MetAsn: 1.142 ± 0.286
1.214MetPro: 1.214 ± 0.284
1.142MetGln: 1.142 ± 0.248
1.356MetArg: 1.356 ± 0.25
2.356MetSer: 2.356 ± 0.399
1.642MetThr: 1.642 ± 0.374
1.428MetVal: 1.428 ± 0.348
0.571MetTrp: 0.571 ± 0.181
0.642MetTyr: 0.642 ± 0.222
0.0MetXaa: 0.0 ± 0.0
Asn
3.569AsnAla: 3.569 ± 0.565
0.785AsnCys: 0.785 ± 0.261
2.213AsnAsp: 2.213 ± 0.361
3.212AsnGlu: 3.212 ± 0.502
1.57AsnPhe: 1.57 ± 0.35
3.426AsnGly: 3.426 ± 0.6
1.142AsnHis: 1.142 ± 0.244
2.855AsnIle: 2.855 ± 0.446
1.356AsnLys: 1.356 ± 0.266
3.069AsnLeu: 3.069 ± 0.417
1.214AsnMet: 1.214 ± 0.275
1.642AsnAsn: 1.642 ± 0.354
2.784AsnPro: 2.784 ± 0.467
2.141AsnGln: 2.141 ± 0.486
2.784AsnArg: 2.784 ± 0.397
1.856AsnSer: 1.856 ± 0.326
2.784AsnThr: 2.784 ± 0.513
2.57AsnVal: 2.57 ± 0.364
0.928AsnTrp: 0.928 ± 0.205
1.356AsnTyr: 1.356 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
3.498ProAla: 3.498 ± 0.58
0.357ProCys: 0.357 ± 0.148
3.069ProAsp: 3.069 ± 0.413
4.783ProGlu: 4.783 ± 0.692
2.356ProPhe: 2.356 ± 0.401
3.498ProGly: 3.498 ± 0.566
0.785ProHis: 0.785 ± 0.251
1.642ProIle: 1.642 ± 0.299
2.641ProLys: 2.641 ± 0.432
2.141ProLeu: 2.141 ± 0.375
0.714ProMet: 0.714 ± 0.218
2.213ProAsn: 2.213 ± 0.375
1.642ProPro: 1.642 ± 0.521
2.07ProGln: 2.07 ± 0.459
1.499ProArg: 1.499 ± 0.318
2.07ProSer: 2.07 ± 0.36
2.57ProThr: 2.57 ± 0.364
2.57ProVal: 2.57 ± 0.458
0.785ProTrp: 0.785 ± 0.17
1.642ProTyr: 1.642 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
4.854GlnAla: 4.854 ± 0.94
0.214GlnCys: 0.214 ± 0.128
2.141GlnAsp: 2.141 ± 0.393
3.426GlnGlu: 3.426 ± 0.502
1.214GlnPhe: 1.214 ± 0.267
2.927GlnGly: 2.927 ± 0.553
0.428GlnHis: 0.428 ± 0.161
1.428GlnIle: 1.428 ± 0.341
1.642GlnLys: 1.642 ± 0.367
2.855GlnLeu: 2.855 ± 0.499
1.713GlnMet: 1.713 ± 0.381
1.856GlnAsn: 1.856 ± 0.376
0.928GlnPro: 0.928 ± 0.284
1.927GlnGln: 1.927 ± 0.463
3.569GlnArg: 3.569 ± 0.561
2.07GlnSer: 2.07 ± 0.418
2.141GlnThr: 2.141 ± 0.428
3.426GlnVal: 3.426 ± 0.431
0.571GlnTrp: 0.571 ± 0.247
1.142GlnTyr: 1.142 ± 0.327
0.0GlnXaa: 0.0 ± 0.0
Arg
4.997ArgAla: 4.997 ± 0.756
0.571ArgCys: 0.571 ± 0.206
3.426ArgAsp: 3.426 ± 0.477
3.212ArgGlu: 3.212 ± 0.592
1.999ArgPhe: 1.999 ± 0.454
4.854ArgGly: 4.854 ± 0.58
1.142ArgHis: 1.142 ± 0.282
3.926ArgIle: 3.926 ± 0.508
4.14ArgLys: 4.14 ± 0.768
5.568ArgLeu: 5.568 ± 0.611
2.07ArgMet: 2.07 ± 0.363
3.141ArgAsn: 3.141 ± 0.482
2.07ArgPro: 2.07 ± 0.399
2.784ArgGln: 2.784 ± 0.399
3.926ArgArg: 3.926 ± 0.603
3.355ArgSer: 3.355 ± 0.459
1.785ArgThr: 1.785 ± 0.35
4.783ArgVal: 4.783 ± 0.548
0.999ArgTrp: 0.999 ± 0.302
1.285ArgTyr: 1.285 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
4.426SerAla: 4.426 ± 0.578
0.714SerCys: 0.714 ± 0.254
3.355SerAsp: 3.355 ± 0.516
4.568SerGlu: 4.568 ± 0.611
1.927SerPhe: 1.927 ± 0.442
5.354SerGly: 5.354 ± 0.495
1.428SerHis: 1.428 ± 0.305
3.498SerIle: 3.498 ± 0.471
3.855SerLys: 3.855 ± 0.648
4.212SerLeu: 4.212 ± 0.633
1.214SerMet: 1.214 ± 0.307
2.498SerAsn: 2.498 ± 0.372
2.213SerPro: 2.213 ± 0.428
2.498SerGln: 2.498 ± 0.456
2.57SerArg: 2.57 ± 0.378
3.855SerSer: 3.855 ± 0.562
2.284SerThr: 2.284 ± 0.407
4.069SerVal: 4.069 ± 0.514
1.214SerTrp: 1.214 ± 0.291
1.856SerTyr: 1.856 ± 0.45
0.0SerXaa: 0.0 ± 0.0
Thr
4.354ThrAla: 4.354 ± 0.518
0.357ThrCys: 0.357 ± 0.166
2.07ThrAsp: 2.07 ± 0.469
2.998ThrGlu: 2.998 ± 0.366
2.284ThrPhe: 2.284 ± 0.443
4.711ThrGly: 4.711 ± 0.627
1.428ThrHis: 1.428 ± 0.288
2.641ThrIle: 2.641 ± 0.493
2.356ThrLys: 2.356 ± 0.38
4.854ThrLeu: 4.854 ± 0.59
1.285ThrMet: 1.285 ± 0.284
1.713ThrAsn: 1.713 ± 0.34
3.712ThrPro: 3.712 ± 0.624
2.141ThrGln: 2.141 ± 0.358
3.284ThrArg: 3.284 ± 0.408
2.57ThrSer: 2.57 ± 0.414
2.498ThrThr: 2.498 ± 0.523
3.498ThrVal: 3.498 ± 0.569
0.642ThrTrp: 0.642 ± 0.27
1.214ThrTyr: 1.214 ± 0.309
0.0ThrXaa: 0.0 ± 0.0
Val
4.497ValAla: 4.497 ± 0.566
0.714ValCys: 0.714 ± 0.197
4.711ValAsp: 4.711 ± 0.541
4.711ValGlu: 4.711 ± 0.592
3.141ValPhe: 3.141 ± 0.419
5.425ValGly: 5.425 ± 0.51
1.927ValHis: 1.927 ± 0.424
3.069ValIle: 3.069 ± 0.461
3.498ValLys: 3.498 ± 0.374
4.925ValLeu: 4.925 ± 0.683
1.856ValMet: 1.856 ± 0.393
3.569ValAsn: 3.569 ± 0.452
2.213ValPro: 2.213 ± 0.34
1.856ValGln: 1.856 ± 0.382
4.568ValArg: 4.568 ± 0.552
5.211ValSer: 5.211 ± 0.683
4.783ValThr: 4.783 ± 0.642
5.853ValVal: 5.853 ± 0.721
0.857ValTrp: 0.857 ± 0.256
3.141ValTyr: 3.141 ± 0.52
0.0ValXaa: 0.0 ± 0.0
Trp
1.142TrpAla: 1.142 ± 0.299
0.357TrpCys: 0.357 ± 0.16
1.285TrpAsp: 1.285 ± 0.253
1.499TrpGlu: 1.499 ± 0.324
0.571TrpPhe: 0.571 ± 0.181
1.142TrpGly: 1.142 ± 0.244
0.0TrpHis: 0.0 ± 0.0
0.714TrpIle: 0.714 ± 0.24
1.428TrpLys: 1.428 ± 0.34
1.142TrpLeu: 1.142 ± 0.269
0.571TrpMet: 0.571 ± 0.174
0.642TrpAsn: 0.642 ± 0.25
0.785TrpPro: 0.785 ± 0.296
0.5TrpGln: 0.5 ± 0.185
1.071TrpArg: 1.071 ± 0.262
0.999TrpSer: 0.999 ± 0.313
0.857TrpThr: 0.857 ± 0.193
0.999TrpVal: 0.999 ± 0.336
0.357TrpTrp: 0.357 ± 0.182
0.642TrpTyr: 0.642 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.856TyrAla: 1.856 ± 0.34
0.571TyrCys: 0.571 ± 0.21
2.713TyrAsp: 2.713 ± 0.447
1.999TyrGlu: 1.999 ± 0.381
1.214TyrPhe: 1.214 ± 0.252
2.57TyrGly: 2.57 ± 0.391
0.785TyrHis: 0.785 ± 0.245
1.713TyrIle: 1.713 ± 0.398
1.785TyrLys: 1.785 ± 0.396
2.427TyrLeu: 2.427 ± 0.439
0.785TyrMet: 0.785 ± 0.287
1.785TyrAsn: 1.785 ± 0.321
1.642TyrPro: 1.642 ± 0.35
1.142TyrGln: 1.142 ± 0.248
1.927TyrArg: 1.927 ± 0.328
1.927TyrSer: 1.927 ± 0.386
1.642TyrThr: 1.642 ± 0.363
2.427TyrVal: 2.427 ± 0.377
0.357TyrTrp: 0.357 ± 0.2
1.142TyrTyr: 1.142 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (14010 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski