Amino acid dipepetide frequency for Escherichia phage G AB-2017

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.418AlaAla: 10.418 ± 1.699
0.795AlaCys: 0.795 ± 0.257
6.999AlaAsp: 6.999 ± 0.83
7.953AlaGlu: 7.953 ± 1.111
4.533AlaPhe: 4.533 ± 0.642
7.555AlaGly: 7.555 ± 0.705
1.193AlaHis: 1.193 ± 0.355
5.169AlaIle: 5.169 ± 0.864
6.442AlaLys: 6.442 ± 0.83
8.51AlaLeu: 8.51 ± 0.823
1.909AlaMet: 1.909 ± 0.406
3.499AlaAsn: 3.499 ± 0.475
3.499AlaPro: 3.499 ± 0.471
3.499AlaGln: 3.499 ± 0.933
3.738AlaArg: 3.738 ± 0.544
5.408AlaSer: 5.408 ± 0.965
4.851AlaThr: 4.851 ± 0.702
6.999AlaVal: 6.999 ± 0.817
1.909AlaTrp: 1.909 ± 0.44
2.943AlaTyr: 2.943 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
1.113CysAla: 1.113 ± 0.328
0.08CysCys: 0.08 ± 0.083
0.954CysAsp: 0.954 ± 0.277
1.272CysGlu: 1.272 ± 0.415
0.318CysPhe: 0.318 ± 0.163
1.193CysGly: 1.193 ± 0.342
0.239CysHis: 0.239 ± 0.156
0.477CysIle: 0.477 ± 0.203
0.716CysLys: 0.716 ± 0.218
0.557CysLeu: 0.557 ± 0.241
0.08CysMet: 0.08 ± 0.076
0.318CysAsn: 0.318 ± 0.203
0.239CysPro: 0.239 ± 0.138
0.239CysGln: 0.239 ± 0.128
0.795CysArg: 0.795 ± 0.34
0.159CysSer: 0.159 ± 0.121
0.795CysThr: 0.795 ± 0.337
0.716CysVal: 0.716 ± 0.275
0.159CysTrp: 0.159 ± 0.107
0.636CysTyr: 0.636 ± 0.219
0.0CysXaa: 0.0 ± 0.0
Asp
6.76AspAla: 6.76 ± 0.789
0.636AspCys: 0.636 ± 0.246
4.772AspAsp: 4.772 ± 0.628
4.772AspGlu: 4.772 ± 0.753
2.863AspPhe: 2.863 ± 0.475
6.283AspGly: 6.283 ± 0.854
0.795AspHis: 0.795 ± 0.256
3.738AspIle: 3.738 ± 0.6
3.34AspLys: 3.34 ± 0.448
4.295AspLeu: 4.295 ± 0.762
1.272AspMet: 1.272 ± 0.328
2.863AspAsn: 2.863 ± 0.559
1.272AspPro: 1.272 ± 0.397
1.272AspGln: 1.272 ± 0.381
2.943AspArg: 2.943 ± 0.495
2.624AspSer: 2.624 ± 0.523
4.215AspThr: 4.215 ± 0.562
4.692AspVal: 4.692 ± 0.558
0.875AspTrp: 0.875 ± 0.233
2.386AspTyr: 2.386 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
6.521GluAla: 6.521 ± 0.834
0.716GluCys: 0.716 ± 0.264
3.579GluAsp: 3.579 ± 0.665
5.726GluGlu: 5.726 ± 1.101
2.465GluPhe: 2.465 ± 0.48
4.613GluGly: 4.613 ± 0.61
1.113GluHis: 1.113 ± 0.325
3.42GluIle: 3.42 ± 0.546
4.931GluLys: 4.931 ± 0.826
6.283GluLeu: 6.283 ± 0.679
3.261GluMet: 3.261 ± 0.578
2.386GluAsn: 2.386 ± 0.47
2.068GluPro: 2.068 ± 0.527
3.738GluGln: 3.738 ± 0.746
3.897GluArg: 3.897 ± 0.725
3.102GluSer: 3.102 ± 0.578
3.658GluThr: 3.658 ± 0.789
4.374GluVal: 4.374 ± 0.509
1.113GluTrp: 1.113 ± 0.389
2.624GluTyr: 2.624 ± 0.543
0.0GluXaa: 0.0 ± 0.0
Phe
2.704PheAla: 2.704 ± 0.533
0.557PheCys: 0.557 ± 0.206
3.42PheAsp: 3.42 ± 0.706
2.465PheGlu: 2.465 ± 0.445
1.034PhePhe: 1.034 ± 0.294
3.42PheGly: 3.42 ± 0.544
0.398PheHis: 0.398 ± 0.211
2.306PheIle: 2.306 ± 0.493
2.465PheLys: 2.465 ± 0.431
1.988PheLeu: 1.988 ± 0.464
0.795PheMet: 0.795 ± 0.24
1.909PheAsn: 1.909 ± 0.4
1.352PhePro: 1.352 ± 0.433
0.954PheGln: 0.954 ± 0.328
1.829PheArg: 1.829 ± 0.33
3.181PheSer: 3.181 ± 0.54
2.465PheThr: 2.465 ± 0.455
3.022PheVal: 3.022 ± 0.516
0.636PheTrp: 0.636 ± 0.236
1.272PheTyr: 1.272 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
7.237GlyAla: 7.237 ± 0.824
1.272GlyCys: 1.272 ± 0.302
4.533GlyAsp: 4.533 ± 0.729
5.01GlyGlu: 5.01 ± 0.686
3.499GlyPhe: 3.499 ± 0.56
5.488GlyGly: 5.488 ± 0.694
1.591GlyHis: 1.591 ± 0.407
2.624GlyIle: 2.624 ± 0.467
5.09GlyLys: 5.09 ± 0.872
5.328GlyLeu: 5.328 ± 0.654
2.306GlyMet: 2.306 ± 0.625
3.499GlyAsn: 3.499 ± 0.44
2.147GlyPro: 2.147 ± 0.484
3.102GlyGln: 3.102 ± 0.634
3.658GlyArg: 3.658 ± 0.647
5.249GlySer: 5.249 ± 0.878
4.056GlyThr: 4.056 ± 0.579
5.885GlyVal: 5.885 ± 0.932
1.193GlyTrp: 1.193 ± 0.313
2.863GlyTyr: 2.863 ± 0.601
0.08GlyXaa: 0.08 ± 0.076
His
1.432HisAla: 1.432 ± 0.333
0.477HisCys: 0.477 ± 0.182
1.113HisAsp: 1.113 ± 0.28
1.113HisGlu: 1.113 ± 0.326
0.716HisPhe: 0.716 ± 0.229
1.113HisGly: 1.113 ± 0.334
0.716HisHis: 0.716 ± 0.268
0.875HisIle: 0.875 ± 0.277
1.034HisLys: 1.034 ± 0.371
1.272HisLeu: 1.272 ± 0.342
0.318HisMet: 0.318 ± 0.164
0.557HisAsn: 0.557 ± 0.226
1.034HisPro: 1.034 ± 0.318
0.716HisGln: 0.716 ± 0.288
1.432HisArg: 1.432 ± 0.33
0.557HisSer: 0.557 ± 0.212
0.716HisThr: 0.716 ± 0.363
1.432HisVal: 1.432 ± 0.366
0.08HisTrp: 0.08 ± 0.075
0.557HisTyr: 0.557 ± 0.217
0.08HisXaa: 0.08 ± 0.076
Ile
5.328IleAla: 5.328 ± 1.044
0.795IleCys: 0.795 ± 0.258
3.579IleAsp: 3.579 ± 0.676
3.499IleGlu: 3.499 ± 0.435
1.352IlePhe: 1.352 ± 0.401
3.261IleGly: 3.261 ± 0.46
0.557IleHis: 0.557 ± 0.235
3.022IleIle: 3.022 ± 0.372
3.34IleLys: 3.34 ± 0.618
2.784IleLeu: 2.784 ± 0.481
1.034IleMet: 1.034 ± 0.314
2.943IleAsn: 2.943 ± 0.519
2.863IlePro: 2.863 ± 0.47
1.909IleGln: 1.909 ± 0.458
2.147IleArg: 2.147 ± 0.479
4.136IleSer: 4.136 ± 0.677
4.454IleThr: 4.454 ± 0.691
4.533IleVal: 4.533 ± 0.558
0.636IleTrp: 0.636 ± 0.254
1.511IleTyr: 1.511 ± 0.451
0.0IleXaa: 0.0 ± 0.0
Lys
5.647LysAla: 5.647 ± 0.984
0.636LysCys: 0.636 ± 0.268
3.976LysAsp: 3.976 ± 0.656
4.692LysGlu: 4.692 ± 0.9
2.147LysPhe: 2.147 ± 0.324
3.658LysGly: 3.658 ± 0.468
1.432LysHis: 1.432 ± 0.347
2.306LysIle: 2.306 ± 0.568
3.022LysLys: 3.022 ± 0.529
5.01LysLeu: 5.01 ± 0.678
2.943LysMet: 2.943 ± 0.666
2.306LysAsn: 2.306 ± 0.552
2.227LysPro: 2.227 ± 0.457
2.147LysGln: 2.147 ± 0.452
3.897LysArg: 3.897 ± 0.545
2.784LysSer: 2.784 ± 0.446
4.136LysThr: 4.136 ± 0.537
3.897LysVal: 3.897 ± 0.589
0.875LysTrp: 0.875 ± 0.305
2.465LysTyr: 2.465 ± 0.45
0.0LysXaa: 0.0 ± 0.0
Leu
8.271LeuAla: 8.271 ± 0.828
0.795LeuCys: 0.795 ± 0.259
3.738LeuAsp: 3.738 ± 0.713
5.09LeuGlu: 5.09 ± 0.785
2.306LeuPhe: 2.306 ± 0.54
5.488LeuGly: 5.488 ± 0.616
1.591LeuHis: 1.591 ± 0.434
4.533LeuIle: 4.533 ± 0.654
3.579LeuLys: 3.579 ± 0.64
5.169LeuLeu: 5.169 ± 0.83
1.909LeuMet: 1.909 ± 0.39
3.897LeuAsn: 3.897 ± 0.661
3.817LeuPro: 3.817 ± 0.739
2.704LeuGln: 2.704 ± 0.523
4.136LeuArg: 4.136 ± 0.59
4.533LeuSer: 4.533 ± 0.59
5.647LeuThr: 5.647 ± 0.883
4.215LeuVal: 4.215 ± 0.74
0.954LeuTrp: 0.954 ± 0.341
2.704LeuTyr: 2.704 ± 0.59
0.0LeuXaa: 0.0 ± 0.0
Met
2.943MetAla: 2.943 ± 0.502
0.398MetCys: 0.398 ± 0.148
0.795MetAsp: 0.795 ± 0.292
0.954MetGlu: 0.954 ± 0.26
0.954MetPhe: 0.954 ± 0.292
1.988MetGly: 1.988 ± 0.406
0.398MetHis: 0.398 ± 0.196
1.75MetIle: 1.75 ± 0.477
1.909MetLys: 1.909 ± 0.467
1.988MetLeu: 1.988 ± 0.338
0.557MetMet: 0.557 ± 0.257
0.795MetAsn: 0.795 ± 0.252
1.511MetPro: 1.511 ± 0.275
0.716MetGln: 0.716 ± 0.22
1.352MetArg: 1.352 ± 0.272
2.227MetSer: 2.227 ± 0.396
1.75MetThr: 1.75 ± 0.356
2.227MetVal: 2.227 ± 0.402
0.318MetTrp: 0.318 ± 0.157
0.318MetTyr: 0.318 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
5.01AsnAla: 5.01 ± 0.793
0.636AsnCys: 0.636 ± 0.186
2.386AsnAsp: 2.386 ± 0.517
2.386AsnGlu: 2.386 ± 0.529
1.034AsnPhe: 1.034 ± 0.346
3.976AsnGly: 3.976 ± 0.647
0.557AsnHis: 0.557 ± 0.19
2.147AsnIle: 2.147 ± 0.488
2.545AsnLys: 2.545 ± 0.511
2.704AsnLeu: 2.704 ± 0.406
1.272AsnMet: 1.272 ± 0.36
2.624AsnAsn: 2.624 ± 0.482
1.67AsnPro: 1.67 ± 0.342
1.511AsnGln: 1.511 ± 0.391
2.386AsnArg: 2.386 ± 0.589
2.704AsnSer: 2.704 ± 0.315
2.784AsnThr: 2.784 ± 0.516
3.658AsnVal: 3.658 ± 0.63
0.795AsnTrp: 0.795 ± 0.244
1.193AsnTyr: 1.193 ± 0.343
0.0AsnXaa: 0.0 ± 0.0
Pro
2.863ProAla: 2.863 ± 0.435
0.477ProCys: 0.477 ± 0.184
3.42ProAsp: 3.42 ± 0.564
3.42ProGlu: 3.42 ± 0.516
1.511ProPhe: 1.511 ± 0.446
3.022ProGly: 3.022 ± 0.614
0.557ProHis: 0.557 ± 0.245
1.511ProIle: 1.511 ± 0.342
1.432ProLys: 1.432 ± 0.356
2.704ProLeu: 2.704 ± 0.467
0.875ProMet: 0.875 ± 0.35
1.591ProAsn: 1.591 ± 0.416
0.875ProPro: 0.875 ± 0.297
1.034ProGln: 1.034 ± 0.259
1.352ProArg: 1.352 ± 0.405
2.306ProSer: 2.306 ± 0.343
2.386ProThr: 2.386 ± 0.481
3.897ProVal: 3.897 ± 0.534
0.318ProTrp: 0.318 ± 0.19
1.034ProTyr: 1.034 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
3.817GlnAla: 3.817 ± 0.629
0.398GlnCys: 0.398 ± 0.233
1.909GlnAsp: 1.909 ± 0.393
2.068GlnGlu: 2.068 ± 0.44
1.272GlnPhe: 1.272 ± 0.328
1.75GlnGly: 1.75 ± 0.304
0.954GlnHis: 0.954 ± 0.343
2.386GlnIle: 2.386 ± 0.478
2.943GlnLys: 2.943 ± 0.603
3.579GlnLeu: 3.579 ± 0.612
1.113GlnMet: 1.113 ± 0.283
1.511GlnAsn: 1.511 ± 0.431
1.432GlnPro: 1.432 ± 0.31
2.624GlnGln: 2.624 ± 0.86
2.624GlnArg: 2.624 ± 0.497
1.829GlnSer: 1.829 ± 0.446
2.147GlnThr: 2.147 ± 0.485
2.386GlnVal: 2.386 ± 0.467
0.716GlnTrp: 0.716 ± 0.224
1.591GlnTyr: 1.591 ± 0.428
0.0GlnXaa: 0.0 ± 0.0
Arg
4.136ArgAla: 4.136 ± 0.516
0.318ArgCys: 0.318 ± 0.203
2.943ArgAsp: 2.943 ± 0.428
3.579ArgGlu: 3.579 ± 0.684
1.909ArgPhe: 1.909 ± 0.335
3.42ArgGly: 3.42 ± 0.496
1.75ArgHis: 1.75 ± 0.402
3.022ArgIle: 3.022 ± 0.575
3.42ArgLys: 3.42 ± 0.569
4.056ArgLeu: 4.056 ± 0.43
1.829ArgMet: 1.829 ± 0.437
2.624ArgAsn: 2.624 ± 0.385
1.352ArgPro: 1.352 ± 0.33
3.261ArgGln: 3.261 ± 0.65
4.533ArgArg: 4.533 ± 0.614
2.704ArgSer: 2.704 ± 0.398
2.306ArgThr: 2.306 ± 0.363
3.499ArgVal: 3.499 ± 0.463
0.557ArgTrp: 0.557 ± 0.217
1.829ArgTyr: 1.829 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
5.01SerAla: 5.01 ± 0.715
0.159SerCys: 0.159 ± 0.114
3.102SerAsp: 3.102 ± 0.507
3.022SerGlu: 3.022 ± 0.602
2.147SerPhe: 2.147 ± 0.469
6.521SerGly: 6.521 ± 0.715
0.795SerHis: 0.795 ± 0.264
3.181SerIle: 3.181 ± 0.773
3.658SerLys: 3.658 ± 0.599
4.295SerLeu: 4.295 ± 0.442
1.113SerMet: 1.113 ± 0.365
3.022SerAsn: 3.022 ± 0.638
2.306SerPro: 2.306 ± 0.632
1.909SerGln: 1.909 ± 0.458
3.022SerArg: 3.022 ± 0.414
3.022SerSer: 3.022 ± 0.916
3.817SerThr: 3.817 ± 0.599
4.613SerVal: 4.613 ± 0.558
0.716SerTrp: 0.716 ± 0.241
2.147SerTyr: 2.147 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
6.68ThrAla: 6.68 ± 1.13
0.557ThrCys: 0.557 ± 0.204
3.897ThrAsp: 3.897 ± 0.46
4.056ThrGlu: 4.056 ± 0.57
3.022ThrPhe: 3.022 ± 0.522
5.09ThrGly: 5.09 ± 0.971
0.954ThrHis: 0.954 ± 0.29
3.897ThrIle: 3.897 ± 0.62
3.658ThrLys: 3.658 ± 0.556
4.533ThrLeu: 4.533 ± 0.517
0.954ThrMet: 0.954 ± 0.263
2.147ThrAsn: 2.147 ± 0.428
3.579ThrPro: 3.579 ± 0.606
2.147ThrGln: 2.147 ± 0.496
2.545ThrArg: 2.545 ± 0.303
3.499ThrSer: 3.499 ± 0.491
4.136ThrThr: 4.136 ± 0.696
3.42ThrVal: 3.42 ± 0.49
1.034ThrTrp: 1.034 ± 0.301
2.227ThrTyr: 2.227 ± 0.45
0.0ThrXaa: 0.0 ± 0.0
Val
7.158ValAla: 7.158 ± 0.689
0.954ValCys: 0.954 ± 0.282
3.817ValAsp: 3.817 ± 0.53
4.851ValGlu: 4.851 ± 0.907
2.386ValPhe: 2.386 ± 0.462
4.215ValGly: 4.215 ± 0.552
0.636ValHis: 0.636 ± 0.2
5.169ValIle: 5.169 ± 0.701
4.215ValLys: 4.215 ± 0.558
5.726ValLeu: 5.726 ± 0.903
1.193ValMet: 1.193 ± 0.283
3.34ValAsn: 3.34 ± 0.697
1.829ValPro: 1.829 ± 0.47
3.34ValGln: 3.34 ± 0.513
3.976ValArg: 3.976 ± 0.5
4.692ValSer: 4.692 ± 0.673
5.249ValThr: 5.249 ± 0.769
4.374ValVal: 4.374 ± 0.779
0.875ValTrp: 0.875 ± 0.358
2.943ValTyr: 2.943 ± 0.585
0.08ValXaa: 0.08 ± 0.076
Trp
1.193TrpAla: 1.193 ± 0.439
0.08TrpCys: 0.08 ± 0.078
0.954TrpAsp: 0.954 ± 0.31
0.716TrpGlu: 0.716 ± 0.266
1.034TrpPhe: 1.034 ± 0.344
0.636TrpGly: 0.636 ± 0.222
0.318TrpHis: 0.318 ± 0.174
0.477TrpIle: 0.477 ± 0.182
0.557TrpLys: 0.557 ± 0.196
1.75TrpLeu: 1.75 ± 0.29
0.636TrpMet: 0.636 ± 0.204
0.636TrpAsn: 0.636 ± 0.206
0.318TrpPro: 0.318 ± 0.215
0.716TrpGln: 0.716 ± 0.287
1.034TrpArg: 1.034 ± 0.298
0.954TrpSer: 0.954 ± 0.367
0.636TrpThr: 0.636 ± 0.213
0.795TrpVal: 0.795 ± 0.209
0.08TrpTrp: 0.08 ± 0.084
0.557TrpTyr: 0.557 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.579TyrAla: 3.579 ± 0.597
0.318TyrCys: 0.318 ± 0.163
2.784TyrAsp: 2.784 ± 0.682
2.943TyrGlu: 2.943 ± 0.436
1.67TyrPhe: 1.67 ± 0.358
2.943TyrGly: 2.943 ± 0.531
0.795TyrHis: 0.795 ± 0.252
1.67TyrIle: 1.67 ± 0.354
1.909TyrLys: 1.909 ± 0.439
2.465TyrLeu: 2.465 ± 0.346
0.398TyrMet: 0.398 ± 0.149
1.511TyrAsn: 1.511 ± 0.308
1.193TyrPro: 1.193 ± 0.44
1.511TyrGln: 1.511 ± 0.312
1.67TyrArg: 1.67 ± 0.357
1.988TyrSer: 1.988 ± 0.378
1.829TyrThr: 1.829 ± 0.378
2.386TyrVal: 2.386 ± 0.459
0.239TyrTrp: 0.239 ± 0.123
1.272TyrTyr: 1.272 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.08XaaGly: 0.08 ± 0.076
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.08XaaLeu: 0.08 ± 0.076
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.08XaaArg: 0.08 ± 0.076
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.159XaaXaa: 0.159 ± 0.152
Statistics based on 68 proteins (12575 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski