Escherichia phage C130_2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Giessenvirus; Escherichia virus C1302

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384ZRQ5|A0A384ZRQ5_9CAUD Uncharacterized protein OS=Escherichia phage C130_2 OX=2234093 GN=1302_0012 PE=4 SV=1
MM1 pKa = 7.49GGIAYY6 pKa = 7.16QVCGAVSGPLRR17 pKa = 11.84IEE19 pKa = 3.76KK20 pKa = 10.53GITIDD25 pKa = 4.27GEE27 pKa = 4.35YY28 pKa = 10.69EE29 pKa = 3.69RR30 pKa = 11.84LAEE33 pKa = 4.07QEE35 pKa = 4.02ASGIDD40 pKa = 3.45YY41 pKa = 9.01AAPEE45 pKa = 4.09EE46 pKa = 4.27QGRR49 pKa = 11.84GAAIEE54 pKa = 5.0GEE56 pKa = 4.54DD57 pKa = 4.08LPPPAGLPDD66 pKa = 3.32

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384ZRQ6|A0A384ZRQ6_9CAUD Uncharacterized protein OS=Escherichia phage C130_2 OX=2234093 GN=1302_0016 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.32RR3 pKa = 11.84GIISALVNMVISSKK17 pKa = 10.73QKK19 pKa = 10.57AEE21 pKa = 4.32VIPEE25 pKa = 5.06PIQKK29 pKa = 8.4MRR31 pKa = 11.84ADD33 pKa = 4.51NIRR36 pKa = 11.84QEE38 pKa = 4.61AINAGIVKK46 pKa = 9.48PRR48 pKa = 11.84AAMQRR53 pKa = 11.84AMMGTPPDD61 pKa = 3.52VPDD64 pKa = 3.67RR65 pKa = 11.84KK66 pKa = 10.29EE67 pKa = 3.88GEE69 pKa = 3.94TRR71 pKa = 11.84KK72 pKa = 9.8EE73 pKa = 3.62KK74 pKa = 10.3KK75 pKa = 10.02ARR77 pKa = 11.84LIAKK81 pKa = 7.73GTASLMRR88 pKa = 11.84RR89 pKa = 11.84GYY91 pKa = 9.48SRR93 pKa = 11.84KK94 pKa = 8.92EE95 pKa = 3.5AEE97 pKa = 4.78AIAKK101 pKa = 10.0RR102 pKa = 11.84SYY104 pKa = 10.88AEE106 pKa = 3.84AVRR109 pKa = 11.84SVGNAAQGRR118 pKa = 4.05

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13031

44

1021

220.9

24.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.043 ± 0.597

1.166 ± 0.199

6.385 ± 0.195

6.208 ± 0.354

3.246 ± 0.142

8.065 ± 0.367

1.796 ± 0.187

5.349 ± 0.263

5.633 ± 0.353

7.083 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.12

4.297 ± 0.246

4.704 ± 0.251

5.026 ± 0.472

6.308 ± 0.249

4.766 ± 0.185

5.702 ± 0.321

5.97 ± 0.296

1.535 ± 0.121

3.062 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski