Lactobacillus phage Sabazios

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coetzeevirus; unclassified Coetzeevirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8HZJ8|A0A3Q8HZJ8_9CAUD Adenine-specific methyltransferase OS=Lactobacillus phage Sabazios OX=2315482 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.02RR4 pKa = 11.84AQRR7 pKa = 11.84VLDD10 pKa = 3.74ALMEE14 pKa = 4.35FDD16 pKa = 4.51SNFGVGYY23 pKa = 8.85PIYY26 pKa = 10.03SIHH29 pKa = 7.09HH30 pKa = 6.43FFDD33 pKa = 2.88KK34 pKa = 10.72CYY36 pKa = 10.88NGNEE40 pKa = 3.91YY41 pKa = 10.98DD42 pKa = 5.04DD43 pKa = 6.02DD44 pKa = 4.66YY45 pKa = 11.95EE46 pKa = 4.34NLTIDD51 pKa = 4.55EE52 pKa = 4.47EE53 pKa = 4.44KK54 pKa = 10.8QVIKK58 pKa = 10.79KK59 pKa = 9.94FLEE62 pKa = 3.81FAYY65 pKa = 10.45

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7UNL9|A0A3S7UNL9_9CAUD Uncharacterized protein OS=Lactobacillus phage Sabazios OX=2315482 PE=4 SV=1
MM1 pKa = 6.88WWFIKK6 pKa = 10.1FALVISFIIGMAIAALPYY24 pKa = 10.43LIALAAGITLIVLLIKK40 pKa = 10.2LLKK43 pKa = 10.01RR44 pKa = 11.84KK45 pKa = 9.67GVII48 pKa = 3.35

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11865

29

1066

191.4

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.723 ± 0.954

0.565 ± 0.132

7.038 ± 0.494

5.807 ± 0.466

3.877 ± 0.193

7.467 ± 0.53

1.5 ± 0.133

6.094 ± 0.215

6.97 ± 0.329

7.644 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.034 ± 0.235

5.588 ± 0.316

2.866 ± 0.167

3.43 ± 0.17

3.953 ± 0.344

6.313 ± 0.383

6.81 ± 0.259

6.7 ± 0.246

1.07 ± 0.098

4.551 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski