Amino acid dipepetide frequency for Arthrobacter phage Tweety19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.448AlaAla: 19.448 ± 2.186
0.714AlaCys: 0.714 ± 0.233
8.89AlaAsp: 8.89 ± 0.893
10.002AlaGlu: 10.002 ± 1.065
3.493AlaPhe: 3.493 ± 0.677
10.002AlaGly: 10.002 ± 1.29
2.064AlaHis: 2.064 ± 0.333
5.715AlaIle: 5.715 ± 0.846
5.477AlaLys: 5.477 ± 0.766
13.732AlaLeu: 13.732 ± 1.034
2.54AlaMet: 2.54 ± 0.443
2.143AlaAsn: 2.143 ± 0.361
6.588AlaPro: 6.588 ± 0.753
3.175AlaGln: 3.175 ± 0.504
9.208AlaArg: 9.208 ± 1.036
7.541AlaSer: 7.541 ± 0.64
7.223AlaThr: 7.223 ± 0.953
9.763AlaVal: 9.763 ± 0.999
1.826AlaTrp: 1.826 ± 0.397
3.572AlaTyr: 3.572 ± 0.407
0.0AlaXaa: 0.0 ± 0.0
Cys
0.556CysAla: 0.556 ± 0.18
0.0CysCys: 0.0 ± 0.0
0.476CysAsp: 0.476 ± 0.193
0.476CysGlu: 0.476 ± 0.166
0.159CysPhe: 0.159 ± 0.1
0.714CysGly: 0.714 ± 0.222
0.159CysHis: 0.159 ± 0.111
0.079CysIle: 0.079 ± 0.086
0.159CysLys: 0.159 ± 0.126
0.238CysLeu: 0.238 ± 0.146
0.079CysMet: 0.079 ± 0.08
0.159CysAsn: 0.159 ± 0.092
0.556CysPro: 0.556 ± 0.192
0.238CysGln: 0.238 ± 0.135
0.714CysArg: 0.714 ± 0.276
0.476CysSer: 0.476 ± 0.212
0.318CysThr: 0.318 ± 0.161
0.159CysVal: 0.159 ± 0.132
0.159CysTrp: 0.159 ± 0.111
0.079CysTyr: 0.079 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
8.573AspAla: 8.573 ± 0.817
0.318AspCys: 0.318 ± 0.189
4.366AspAsp: 4.366 ± 0.589
4.683AspGlu: 4.683 ± 0.592
1.905AspPhe: 1.905 ± 0.355
6.985AspGly: 6.985 ± 0.91
1.191AspHis: 1.191 ± 0.299
1.746AspIle: 1.746 ± 0.314
1.508AspLys: 1.508 ± 0.3
6.35AspLeu: 6.35 ± 0.796
0.635AspMet: 0.635 ± 0.192
1.429AspAsn: 1.429 ± 0.285
3.493AspPro: 3.493 ± 0.481
1.191AspGln: 1.191 ± 0.264
3.334AspArg: 3.334 ± 0.567
3.254AspSer: 3.254 ± 0.586
3.334AspThr: 3.334 ± 0.526
4.366AspVal: 4.366 ± 0.431
0.873AspTrp: 0.873 ± 0.24
1.508AspTyr: 1.508 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
10.081GluAla: 10.081 ± 1.199
0.318GluCys: 0.318 ± 0.161
3.096GluAsp: 3.096 ± 0.527
5.318GluGlu: 5.318 ± 0.614
1.667GluPhe: 1.667 ± 0.339
5.477GluGly: 5.477 ± 0.501
1.349GluHis: 1.349 ± 0.301
3.651GluIle: 3.651 ± 0.49
2.778GluLys: 2.778 ± 0.509
6.112GluLeu: 6.112 ± 0.829
0.873GluMet: 0.873 ± 0.259
2.223GluAsn: 2.223 ± 0.334
3.096GluPro: 3.096 ± 0.565
1.27GluGln: 1.27 ± 0.33
4.842GluArg: 4.842 ± 0.582
2.699GluSer: 2.699 ± 0.479
2.54GluThr: 2.54 ± 0.427
5.795GluVal: 5.795 ± 0.557
1.588GluTrp: 1.588 ± 0.339
1.508GluTyr: 1.508 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.254PheAla: 3.254 ± 0.464
0.318PheCys: 0.318 ± 0.159
2.302PheAsp: 2.302 ± 0.513
2.461PheGlu: 2.461 ± 0.419
1.429PhePhe: 1.429 ± 0.423
3.096PheGly: 3.096 ± 0.462
0.714PheHis: 0.714 ± 0.198
1.111PheIle: 1.111 ± 0.238
0.794PheLys: 0.794 ± 0.29
2.223PheLeu: 2.223 ± 0.491
1.111PheMet: 1.111 ± 0.291
0.953PheAsn: 0.953 ± 0.337
1.588PhePro: 1.588 ± 0.32
1.111PheGln: 1.111 ± 0.356
2.381PheArg: 2.381 ± 0.559
1.667PheSer: 1.667 ± 0.334
2.143PheThr: 2.143 ± 0.369
2.54PheVal: 2.54 ± 0.379
0.238PheTrp: 0.238 ± 0.124
1.27PheTyr: 1.27 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
9.922GlyAla: 9.922 ± 1.216
0.397GlyCys: 0.397 ± 0.17
4.683GlyAsp: 4.683 ± 0.595
5.715GlyGlu: 5.715 ± 0.666
3.493GlyPhe: 3.493 ± 0.564
6.191GlyGly: 6.191 ± 0.763
1.429GlyHis: 1.429 ± 0.296
4.286GlyIle: 4.286 ± 0.878
3.969GlyLys: 3.969 ± 0.56
7.223GlyLeu: 7.223 ± 0.871
1.429GlyMet: 1.429 ± 0.343
2.619GlyAsn: 2.619 ± 0.673
3.969GlyPro: 3.969 ± 0.614
1.429GlyGln: 1.429 ± 0.274
6.191GlyArg: 6.191 ± 0.488
4.207GlySer: 4.207 ± 0.539
5.239GlyThr: 5.239 ± 0.852
6.668GlyVal: 6.668 ± 0.949
1.588GlyTrp: 1.588 ± 0.366
2.302GlyTyr: 2.302 ± 0.324
0.0GlyXaa: 0.0 ± 0.0
His
2.223HisAla: 2.223 ± 0.411
0.159HisCys: 0.159 ± 0.107
1.588HisAsp: 1.588 ± 0.465
1.111HisGlu: 1.111 ± 0.243
1.191HisPhe: 1.191 ± 0.281
1.191HisGly: 1.191 ± 0.232
0.397HisHis: 0.397 ± 0.179
0.635HisIle: 0.635 ± 0.209
0.714HisLys: 0.714 ± 0.237
1.191HisLeu: 1.191 ± 0.415
0.635HisMet: 0.635 ± 0.228
0.556HisAsn: 0.556 ± 0.218
0.953HisPro: 0.953 ± 0.212
0.556HisGln: 0.556 ± 0.195
1.508HisArg: 1.508 ± 0.306
0.714HisSer: 0.714 ± 0.289
0.556HisThr: 0.556 ± 0.191
0.873HisVal: 0.873 ± 0.212
0.238HisTrp: 0.238 ± 0.131
0.238HisTyr: 0.238 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
5.874IleAla: 5.874 ± 0.717
0.159IleCys: 0.159 ± 0.101
2.143IleAsp: 2.143 ± 0.387
2.778IleGlu: 2.778 ± 0.455
1.746IlePhe: 1.746 ± 0.394
3.413IleGly: 3.413 ± 0.56
1.111IleHis: 1.111 ± 0.248
1.588IleIle: 1.588 ± 0.339
1.27IleLys: 1.27 ± 0.301
3.493IleLeu: 3.493 ± 0.431
0.953IleMet: 0.953 ± 0.224
1.111IleAsn: 1.111 ± 0.37
2.619IlePro: 2.619 ± 0.412
1.905IleGln: 1.905 ± 0.465
3.89IleArg: 3.89 ± 0.513
2.302IleSer: 2.302 ± 0.389
2.858IleThr: 2.858 ± 0.549
2.699IleVal: 2.699 ± 0.514
0.635IleTrp: 0.635 ± 0.253
0.873IleTyr: 0.873 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
5.556LysAla: 5.556 ± 0.758
0.238LysCys: 0.238 ± 0.122
1.826LysAsp: 1.826 ± 0.458
2.461LysGlu: 2.461 ± 0.433
1.111LysPhe: 1.111 ± 0.303
3.016LysGly: 3.016 ± 0.495
0.873LysHis: 0.873 ± 0.223
1.905LysIle: 1.905 ± 0.518
2.461LysLys: 2.461 ± 0.456
3.413LysLeu: 3.413 ± 0.585
0.794LysMet: 0.794 ± 0.231
0.873LysAsn: 0.873 ± 0.274
2.54LysPro: 2.54 ± 0.466
0.794LysGln: 0.794 ± 0.229
2.381LysArg: 2.381 ± 0.416
2.223LysSer: 2.223 ± 0.436
2.461LysThr: 2.461 ± 0.522
3.016LysVal: 3.016 ± 0.476
0.714LysTrp: 0.714 ± 0.218
0.873LysTyr: 0.873 ± 0.25
0.0LysXaa: 0.0 ± 0.0
Leu
12.542LeuAla: 12.542 ± 0.879
0.556LeuCys: 0.556 ± 0.211
5.398LeuAsp: 5.398 ± 0.587
4.286LeuGlu: 4.286 ± 0.665
2.461LeuPhe: 2.461 ± 0.38
7.144LeuGly: 7.144 ± 0.738
1.588LeuHis: 1.588 ± 0.402
4.366LeuIle: 4.366 ± 0.555
2.302LeuLys: 2.302 ± 0.423
6.826LeuLeu: 6.826 ± 0.724
1.588LeuMet: 1.588 ± 0.386
3.175LeuAsn: 3.175 ± 0.371
4.683LeuPro: 4.683 ± 0.615
2.699LeuGln: 2.699 ± 0.5
7.382LeuArg: 7.382 ± 0.917
5.16LeuSer: 5.16 ± 0.704
6.35LeuThr: 6.35 ± 0.651
6.985LeuVal: 6.985 ± 0.501
1.746LeuTrp: 1.746 ± 0.307
1.667LeuTyr: 1.667 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
3.175MetAla: 3.175 ± 0.429
0.159MetCys: 0.159 ± 0.091
0.635MetAsp: 0.635 ± 0.225
1.032MetGlu: 1.032 ± 0.226
0.397MetPhe: 0.397 ± 0.155
0.873MetGly: 0.873 ± 0.277
0.159MetHis: 0.159 ± 0.11
1.191MetIle: 1.191 ± 0.305
1.191MetLys: 1.191 ± 0.402
1.429MetLeu: 1.429 ± 0.267
0.079MetMet: 0.079 ± 0.067
0.556MetAsn: 0.556 ± 0.184
0.953MetPro: 0.953 ± 0.283
0.397MetGln: 0.397 ± 0.151
1.746MetArg: 1.746 ± 0.316
1.905MetSer: 1.905 ± 0.42
2.461MetThr: 2.461 ± 0.439
0.794MetVal: 0.794 ± 0.26
0.0MetTrp: 0.0 ± 0.0
0.159MetTyr: 0.159 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.969AsnAla: 3.969 ± 0.696
0.159AsnCys: 0.159 ± 0.109
1.508AsnAsp: 1.508 ± 0.336
0.714AsnGlu: 0.714 ± 0.249
0.556AsnPhe: 0.556 ± 0.224
3.254AsnGly: 3.254 ± 0.456
0.318AsnHis: 0.318 ± 0.184
0.873AsnIle: 0.873 ± 0.242
0.873AsnLys: 0.873 ± 0.192
2.302AsnLeu: 2.302 ± 0.405
0.476AsnMet: 0.476 ± 0.176
0.873AsnAsn: 0.873 ± 0.333
1.826AsnPro: 1.826 ± 0.392
0.635AsnGln: 0.635 ± 0.166
1.349AsnArg: 1.349 ± 0.301
1.588AsnSer: 1.588 ± 0.333
1.984AsnThr: 1.984 ± 0.423
2.461AsnVal: 2.461 ± 0.486
0.635AsnTrp: 0.635 ± 0.272
0.873AsnTyr: 0.873 ± 0.318
0.0AsnXaa: 0.0 ± 0.0
Pro
7.065ProAla: 7.065 ± 0.949
0.238ProCys: 0.238 ± 0.125
3.493ProAsp: 3.493 ± 0.542
4.445ProGlu: 4.445 ± 0.628
1.905ProPhe: 1.905 ± 0.4
5.318ProGly: 5.318 ± 0.607
0.953ProHis: 0.953 ± 0.225
2.223ProIle: 2.223 ± 0.367
2.223ProLys: 2.223 ± 0.422
4.048ProLeu: 4.048 ± 0.616
1.27ProMet: 1.27 ± 0.289
1.27ProAsn: 1.27 ± 0.26
1.349ProPro: 1.349 ± 0.406
1.111ProGln: 1.111 ± 0.387
2.858ProArg: 2.858 ± 0.482
1.905ProSer: 1.905 ± 0.293
3.334ProThr: 3.334 ± 0.542
4.525ProVal: 4.525 ± 0.714
0.635ProTrp: 0.635 ± 0.213
1.191ProTyr: 1.191 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
2.937GlnAla: 2.937 ± 0.527
0.318GlnCys: 0.318 ± 0.149
1.27GlnAsp: 1.27 ± 0.302
1.191GlnGlu: 1.191 ± 0.279
0.397GlnPhe: 0.397 ± 0.167
1.508GlnGly: 1.508 ± 0.277
0.238GlnHis: 0.238 ± 0.132
1.746GlnIle: 1.746 ± 0.35
1.588GlnLys: 1.588 ± 0.392
1.429GlnLeu: 1.429 ± 0.357
0.476GlnMet: 0.476 ± 0.201
0.794GlnAsn: 0.794 ± 0.246
1.429GlnPro: 1.429 ± 0.324
0.397GlnGln: 0.397 ± 0.145
1.984GlnArg: 1.984 ± 0.554
1.349GlnSer: 1.349 ± 0.267
2.223GlnThr: 2.223 ± 0.421
2.223GlnVal: 2.223 ± 0.427
0.397GlnTrp: 0.397 ± 0.195
0.238GlnTyr: 0.238 ± 0.146
0.0GlnXaa: 0.0 ± 0.0
Arg
7.382ArgAla: 7.382 ± 0.67
0.397ArgCys: 0.397 ± 0.131
4.842ArgAsp: 4.842 ± 0.715
5.795ArgGlu: 5.795 ± 0.833
2.937ArgPhe: 2.937 ± 0.416
3.81ArgGly: 3.81 ± 0.507
1.191ArgHis: 1.191 ± 0.272
2.937ArgIle: 2.937 ± 0.547
3.493ArgLys: 3.493 ± 0.462
6.985ArgLeu: 6.985 ± 0.884
1.905ArgMet: 1.905 ± 0.37
1.905ArgAsn: 1.905 ± 0.356
4.048ArgPro: 4.048 ± 0.591
2.699ArgGln: 2.699 ± 0.513
7.382ArgArg: 7.382 ± 0.964
3.81ArgSer: 3.81 ± 0.601
3.254ArgThr: 3.254 ± 0.57
6.826ArgVal: 6.826 ± 0.778
1.27ArgTrp: 1.27 ± 0.389
2.381ArgTyr: 2.381 ± 0.467
0.0ArgXaa: 0.0 ± 0.0
Ser
6.826SerAla: 6.826 ± 0.833
0.476SerCys: 0.476 ± 0.198
2.937SerAsp: 2.937 ± 0.454
2.699SerGlu: 2.699 ± 0.345
2.064SerPhe: 2.064 ± 0.374
6.826SerGly: 6.826 ± 0.861
0.476SerHis: 0.476 ± 0.222
2.54SerIle: 2.54 ± 0.5
1.588SerLys: 1.588 ± 0.34
5.16SerLeu: 5.16 ± 0.683
1.349SerMet: 1.349 ± 0.364
1.032SerAsn: 1.032 ± 0.288
3.175SerPro: 3.175 ± 0.53
0.873SerGln: 0.873 ± 0.212
3.89SerArg: 3.89 ± 0.551
2.858SerSer: 2.858 ± 0.565
3.254SerThr: 3.254 ± 0.595
3.651SerVal: 3.651 ± 0.441
0.873SerTrp: 0.873 ± 0.239
1.905SerTyr: 1.905 ± 0.495
0.0SerXaa: 0.0 ± 0.0
Thr
8.176ThrAla: 8.176 ± 0.805
0.238ThrCys: 0.238 ± 0.159
3.731ThrAsp: 3.731 ± 0.495
3.175ThrGlu: 3.175 ± 0.649
2.381ThrPhe: 2.381 ± 0.365
5.16ThrGly: 5.16 ± 0.673
0.953ThrHis: 0.953 ± 0.24
2.619ThrIle: 2.619 ± 0.541
2.778ThrLys: 2.778 ± 0.449
5.874ThrLeu: 5.874 ± 0.65
0.794ThrMet: 0.794 ± 0.275
1.429ThrAsn: 1.429 ± 0.313
3.175ThrPro: 3.175 ± 0.541
1.349ThrGln: 1.349 ± 0.312
4.604ThrArg: 4.604 ± 0.638
3.89ThrSer: 3.89 ± 0.585
3.175ThrThr: 3.175 ± 0.475
4.763ThrVal: 4.763 ± 0.599
0.794ThrTrp: 0.794 ± 0.266
1.191ThrTyr: 1.191 ± 0.245
0.0ThrXaa: 0.0 ± 0.0
Val
10.557ValAla: 10.557 ± 0.709
0.556ValCys: 0.556 ± 0.206
4.921ValAsp: 4.921 ± 0.702
4.525ValGlu: 4.525 ± 0.654
2.461ValPhe: 2.461 ± 0.427
5.318ValGly: 5.318 ± 0.746
1.349ValHis: 1.349 ± 0.274
2.619ValIle: 2.619 ± 0.582
2.619ValLys: 2.619 ± 0.403
7.541ValLeu: 7.541 ± 0.581
1.349ValMet: 1.349 ± 0.339
2.699ValAsn: 2.699 ± 0.416
3.81ValPro: 3.81 ± 0.556
1.588ValGln: 1.588 ± 0.345
5.874ValArg: 5.874 ± 0.682
4.128ValSer: 4.128 ± 0.558
5.001ValThr: 5.001 ± 0.518
5.477ValVal: 5.477 ± 0.618
1.032ValTrp: 1.032 ± 0.313
2.619ValTyr: 2.619 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
2.143TrpAla: 2.143 ± 0.409
0.0TrpCys: 0.0 ± 0.0
0.953TrpAsp: 0.953 ± 0.255
1.27TrpGlu: 1.27 ± 0.322
0.318TrpPhe: 0.318 ± 0.123
0.873TrpGly: 0.873 ± 0.243
0.397TrpHis: 0.397 ± 0.161
0.476TrpIle: 0.476 ± 0.165
1.191TrpLys: 1.191 ± 0.266
1.032TrpLeu: 1.032 ± 0.3
0.238TrpMet: 0.238 ± 0.12
0.476TrpAsn: 0.476 ± 0.211
0.397TrpPro: 0.397 ± 0.177
0.397TrpGln: 0.397 ± 0.145
1.667TrpArg: 1.667 ± 0.341
1.588TrpSer: 1.588 ± 0.428
1.191TrpThr: 1.191 ± 0.286
0.635TrpVal: 0.635 ± 0.258
0.159TrpTrp: 0.159 ± 0.094
0.397TrpTyr: 0.397 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.443
0.238TyrCys: 0.238 ± 0.144
1.984TyrAsp: 1.984 ± 0.329
2.143TyrGlu: 2.143 ± 0.446
0.635TyrPhe: 0.635 ± 0.193
2.778TyrGly: 2.778 ± 0.61
0.318TyrHis: 0.318 ± 0.139
1.111TyrIle: 1.111 ± 0.267
0.556TyrLys: 0.556 ± 0.193
2.302TyrLeu: 2.302 ± 0.356
0.635TyrMet: 0.635 ± 0.208
0.953TyrAsn: 0.953 ± 0.348
1.191TyrPro: 1.191 ± 0.323
0.318TyrGln: 0.318 ± 0.139
2.064TyrArg: 2.064 ± 0.339
1.191TyrSer: 1.191 ± 0.38
1.349TyrThr: 1.349 ± 0.27
1.826TyrVal: 1.826 ± 0.418
0.397TyrTrp: 0.397 ± 0.159
0.238TyrTyr: 0.238 ± 0.131
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12599 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski