Amino acid dipepetide frequency for Azoarcus pumilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.382AlaAla: 17.382 ± 0.207	1.402AlaCys: 1.402 ± 0.042	7.381AlaAsp: 7.381 ± 0.105	7.909AlaGlu: 7.909 ± 0.118	4.129AlaPhe: 4.129 ± 0.063	10.929AlaGly: 10.929 ± 0.116	2.898AlaHis: 2.898 ± 0.062	5.927AlaIle: 5.927 ± 0.09	2.645AlaLys: 2.645 ± 0.073	15.208AlaLeu: 15.208 ± 0.169	3.392AlaMet: 3.392 ± 0.063	2.795AlaAsn: 2.795 ± 0.055	5.618AlaPro: 5.618 ± 0.093	4.213AlaGln: 4.213 ± 0.072	11.143AlaArg: 11.143 ± 0.137	6.087AlaSer: 6.087 ± 0.102	5.878AlaThr: 5.878 ± 0.086	8.772AlaVal: 8.772 ± 0.11	1.93AlaTrp: 1.93 ± 0.05	2.33AlaTyr: 2.33 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.298CysAla: 1.298 ± 0.042	0.153CysCys: 0.153 ± 0.012	0.589CysAsp: 0.589 ± 0.021	0.656CysGlu: 0.656 ± 0.024	0.357CysPhe: 0.357 ± 0.018	1.155CysGly: 1.155 ± 0.037	0.295CysHis: 0.295 ± 0.018	0.442CysIle: 0.442 ± 0.021	0.205CysLys: 0.205 ± 0.014	0.9CysLeu: 0.9 ± 0.029	0.179CysMet: 0.179 ± 0.013	0.232CysAsn: 0.232 ± 0.016	0.518CysPro: 0.518 ± 0.028	0.204CysGln: 0.204 ± 0.016	0.742CysArg: 0.742 ± 0.029	0.526CysSer: 0.526 ± 0.025	0.504CysThr: 0.504 ± 0.027	0.797CysVal: 0.797 ± 0.031	0.129CysTrp: 0.129 ± 0.009	0.217CysTyr: 0.217 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	8.445AspAla: 8.445 ± 0.112	0.557AspCys: 0.557 ± 0.023	3.72AspAsp: 3.72 ± 0.068	4.525AspGlu: 4.525 ± 0.083	2.259AspPhe: 2.259 ± 0.06	5.422AspGly: 5.422 ± 0.094	1.292AspHis: 1.292 ± 0.035	2.808AspIle: 2.808 ± 0.064	1.423AspLys: 1.423 ± 0.045	5.644AspLeu: 5.644 ± 0.092	1.322AspMet: 1.322 ± 0.038	1.256AspAsn: 1.256 ± 0.038	3.107AspPro: 3.107 ± 0.067	1.385AspGln: 1.385 ± 0.035	4.029AspArg: 4.029 ± 0.074	2.284AspSer: 2.284 ± 0.05	2.854AspThr: 2.854 ± 0.06	4.415AspVal: 4.415 ± 0.081	1.075AspTrp: 1.075 ± 0.036	1.479AspTyr: 1.479 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.379GluAla: 8.379 ± 0.121	0.564GluCys: 0.564 ± 0.025	3.065GluAsp: 3.065 ± 0.057	3.115GluGlu: 3.115 ± 0.067	1.95GluPhe: 1.95 ± 0.042	4.207GluGly: 4.207 ± 0.068	1.846GluHis: 1.846 ± 0.046	3.271GluIle: 3.271 ± 0.056	1.659GluLys: 1.659 ± 0.048	6.733GluLeu: 6.733 ± 0.102	1.408GluMet: 1.408 ± 0.037	1.45GluAsn: 1.45 ± 0.041	2.852GluPro: 2.852 ± 0.06	2.465GluGln: 2.465 ± 0.059	6.726GluArg: 6.726 ± 0.103	2.909GluSer: 2.909 ± 0.058	2.97GluThr: 2.97 ± 0.064	4.777GluVal: 4.777 ± 0.078	0.827GluTrp: 0.827 ± 0.033	1.28GluTyr: 1.28 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.741PheAla: 4.741 ± 0.073	0.396PheCys: 0.396 ± 0.024	2.939PheAsp: 2.939 ± 0.065	2.515PheGlu: 2.515 ± 0.056	1.332PhePhe: 1.332 ± 0.044	3.591PheGly: 3.591 ± 0.067	0.764PheHis: 0.764 ± 0.029	1.55PheIle: 1.55 ± 0.045	0.747PheLys: 0.747 ± 0.029	3.085PheLeu: 3.085 ± 0.064	0.788PheMet: 0.788 ± 0.029	0.957PheAsn: 0.957 ± 0.036	1.471PhePro: 1.471 ± 0.04	0.833PheGln: 0.833 ± 0.027	2.353PheArg: 2.353 ± 0.045	1.973PheSer: 1.973 ± 0.052	1.699PheThr: 1.699 ± 0.04	3.177PheVal: 3.177 ± 0.065	0.454PheTrp: 0.454 ± 0.023	0.799PheTyr: 0.799 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.096GlyAla: 9.096 ± 0.118	1.02GlyCys: 1.02 ± 0.033	4.58GlyAsp: 4.58 ± 0.08	5.456GlyGlu: 5.456 ± 0.079	3.527GlyPhe: 3.527 ± 0.061	6.824GlyGly: 6.824 ± 0.112	2.067GlyHis: 2.067 ± 0.048	4.196GlyIle: 4.196 ± 0.065	2.627GlyLys: 2.627 ± 0.059	8.542GlyLeu: 8.542 ± 0.122	2.367GlyMet: 2.367 ± 0.051	2.007GlyAsn: 2.007 ± 0.061	2.843GlyPro: 2.843 ± 0.059	2.507GlyGln: 2.507 ± 0.058	6.803GlyArg: 6.803 ± 0.087	4.07GlySer: 4.07 ± 0.061	3.958GlyThr: 3.958 ± 0.078	6.778GlyVal: 6.778 ± 0.104	1.367GlyTrp: 1.367 ± 0.037	2.142GlyTyr: 2.142 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	3.285HisAla: 3.285 ± 0.076	0.325HisCys: 0.325 ± 0.018	1.468HisAsp: 1.468 ± 0.048	1.288HisGlu: 1.288 ± 0.034	0.936HisPhe: 0.936 ± 0.032	2.267HisGly: 2.267 ± 0.056	0.737HisHis: 0.737 ± 0.035	0.955HisIle: 0.955 ± 0.031	0.512HisLys: 0.512 ± 0.024	2.435HisLeu: 2.435 ± 0.056	0.486HisMet: 0.486 ± 0.025	0.498HisAsn: 0.498 ± 0.024	1.584HisPro: 1.584 ± 0.041	0.608HisGln: 0.608 ± 0.025	1.867HisArg: 1.867 ± 0.043	0.913HisSer: 0.913 ± 0.035	1.004HisThr: 1.004 ± 0.031	1.805HisVal: 1.805 ± 0.041	0.427HisTrp: 0.427 ± 0.018	0.696HisTyr: 0.696 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.772IleAla: 6.772 ± 0.094	0.438IleCys: 0.438 ± 0.019	3.697IleAsp: 3.697 ± 0.062	3.837IleGlu: 3.837 ± 0.067	1.441IlePhe: 1.441 ± 0.039	4.487IleGly: 4.487 ± 0.074	1.012IleHis: 1.012 ± 0.032	1.678IleIle: 1.678 ± 0.05	1.153IleLys: 1.153 ± 0.035	3.78IleLeu: 3.78 ± 0.072	0.747IleMet: 0.747 ± 0.03	1.277IleAsn: 1.277 ± 0.035	2.175IlePro: 2.175 ± 0.052	1.021IleGln: 1.021 ± 0.036	3.104IleArg: 3.104 ± 0.053	2.249IleSer: 2.249 ± 0.056	2.373IleThr: 2.373 ± 0.049	4.301IleVal: 4.301 ± 0.08	0.404IleTrp: 0.404 ± 0.02	0.949IleTyr: 0.949 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	2.78LysAla: 2.78 ± 0.062	0.188LysCys: 0.188 ± 0.015	1.195LysAsp: 1.195 ± 0.042	1.087LysGlu: 1.087 ± 0.037	0.693LysPhe: 0.693 ± 0.03	1.823LysGly: 1.823 ± 0.052	0.62LysHis: 0.62 ± 0.029	1.219LysIle: 1.219 ± 0.036	0.948LysLys: 0.948 ± 0.042	2.722LysLeu: 2.722 ± 0.057	0.651LysMet: 0.651 ± 0.029	0.714LysAsn: 0.714 ± 0.031	1.503LysPro: 1.503 ± 0.039	0.84LysGln: 0.84 ± 0.033	2.022LysArg: 2.022 ± 0.05	1.488LysSer: 1.488 ± 0.048	1.319LysThr: 1.319 ± 0.038	1.876LysVal: 1.876 ± 0.052	0.278LysTrp: 0.278 ± 0.017	0.552LysTyr: 0.552 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	15.583LeuAla: 15.583 ± 0.177	1.066LeuCys: 1.066 ± 0.033	6.724LeuAsp: 6.724 ± 0.088	6.313LeuGlu: 6.313 ± 0.085	3.891LeuPhe: 3.891 ± 0.079	8.402LeuGly: 8.402 ± 0.13	2.361LeuHis: 2.361 ± 0.049	4.733LeuIle: 4.733 ± 0.092	2.545LeuLys: 2.545 ± 0.059	10.928LeuLeu: 10.928 ± 0.16	2.424LeuMet: 2.424 ± 0.057	2.255LeuAsn: 2.255 ± 0.055	5.887LeuPro: 5.887 ± 0.087	3.026LeuGln: 3.026 ± 0.062	8.607LeuArg: 8.607 ± 0.112	5.542LeuSer: 5.542 ± 0.081	5.022LeuThr: 5.022 ± 0.081	8.036LeuVal: 8.036 ± 0.106	1.292LeuTrp: 1.292 ± 0.043	2.088LeuTyr: 2.088 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.646MetAla: 2.646 ± 0.056	0.173MetCys: 0.173 ± 0.014	1.14MetAsp: 1.14 ± 0.036	1.048MetGlu: 1.048 ± 0.036	0.767MetPhe: 0.767 ± 0.035	1.645MetGly: 1.645 ± 0.047	0.576MetHis: 0.576 ± 0.022	1.136MetIle: 1.136 ± 0.039	0.963MetLys: 0.963 ± 0.031	2.8MetLeu: 2.8 ± 0.057	0.623MetMet: 0.623 ± 0.025	0.851MetAsn: 0.851 ± 0.033	1.429MetPro: 1.429 ± 0.038	0.955MetGln: 0.955 ± 0.033	2.068MetArg: 2.068 ± 0.05	1.685MetSer: 1.685 ± 0.043	1.42MetThr: 1.42 ± 0.037	1.619MetVal: 1.619 ± 0.038	0.244MetTrp: 0.244 ± 0.016	0.397MetTyr: 0.397 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.878AsnAla: 2.878 ± 0.064	0.256AsnCys: 0.256 ± 0.017	1.306AsnAsp: 1.306 ± 0.04	1.229AsnGlu: 1.229 ± 0.033	0.875AsnPhe: 0.875 ± 0.029	1.934AsnGly: 1.934 ± 0.049	0.523AsnHis: 0.523 ± 0.021	1.169AsnIle: 1.169 ± 0.033	0.568AsnLys: 0.568 ± 0.022	2.505AsnLeu: 2.505 ± 0.055	0.512AsnMet: 0.512 ± 0.026	0.569AsnAsn: 0.569 ± 0.03	1.618AsnPro: 1.618 ± 0.043	0.672AsnGln: 0.672 ± 0.028	1.85AsnArg: 1.85 ± 0.044	0.918AsnSer: 0.918 ± 0.033	1.194AsnThr: 1.194 ± 0.036	1.843AsnVal: 1.843 ± 0.049	0.327AsnTrp: 0.327 ± 0.018	0.599AsnTyr: 0.599 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.328ProAla: 6.328 ± 0.098	0.428ProCys: 0.428 ± 0.022	3.739ProAsp: 3.739 ± 0.062	3.808ProGlu: 3.808 ± 0.069	1.732ProPhe: 1.732 ± 0.04	4.348ProGly: 4.348 ± 0.064	1.115ProHis: 1.115 ± 0.036	1.794ProIle: 1.794 ± 0.037	1.075ProLys: 1.075 ± 0.039	5.045ProLeu: 5.045 ± 0.077	1.17ProMet: 1.17 ± 0.037	1.101ProAsn: 1.101 ± 0.034	2.666ProPro: 2.666 ± 0.066	1.776ProGln: 1.776 ± 0.045	3.211ProArg: 3.211 ± 0.068	2.294ProSer: 2.294 ± 0.051	2.29ProThr: 2.29 ± 0.051	4.141ProVal: 4.141 ± 0.063	0.674ProTrp: 0.674 ± 0.029	1.028ProTyr: 1.028 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.95GlnAla: 3.95 ± 0.078	0.278GlnCys: 0.278 ± 0.017	1.316GlnAsp: 1.316 ± 0.035	1.375GlnGlu: 1.375 ± 0.041	0.999GlnPhe: 0.999 ± 0.035	2.254GlnGly: 2.254 ± 0.047	0.759GlnHis: 0.759 ± 0.029	1.572GlnIle: 1.572 ± 0.044	0.777GlnLys: 0.777 ± 0.032	3.128GlnLeu: 3.128 ± 0.06	0.845GlnMet: 0.845 ± 0.031	0.634GlnAsn: 0.634 ± 0.026	1.701GlnPro: 1.701 ± 0.049	1.201GlnGln: 1.201 ± 0.042	2.954GlnArg: 2.954 ± 0.067	1.577GlnSer: 1.577 ± 0.039	1.483GlnThr: 1.483 ± 0.045	2.333GlnVal: 2.333 ± 0.049	0.465GlnTrp: 0.465 ± 0.025	0.626GlnTyr: 0.626 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.416ArgAla: 9.416 ± 0.112	0.724ArgCys: 0.724 ± 0.032	4.73ArgAsp: 4.73 ± 0.075	6.073ArgGlu: 6.073 ± 0.095	3.291ArgPhe: 3.291 ± 0.057	5.601ArgGly: 5.601 ± 0.089	2.293ArgHis: 2.293 ± 0.056	4.62ArgIle: 4.62 ± 0.067	1.988ArgLys: 1.988 ± 0.05	9.205ArgLeu: 9.205 ± 0.119	2.299ArgMet: 2.299 ± 0.049	1.992ArgAsn: 1.992 ± 0.04	3.574ArgPro: 3.574 ± 0.06	2.495ArgGln: 2.495 ± 0.061	6.976ArgArg: 6.976 ± 0.129	3.794ArgSer: 3.794 ± 0.071	3.32ArgThr: 3.32 ± 0.06	6.208ArgVal: 6.208 ± 0.082	1.189ArgTrp: 1.189 ± 0.038	1.899ArgTyr: 1.899 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	5.926SerAla: 5.926 ± 0.08	0.455SerCys: 0.455 ± 0.022	2.75SerAsp: 2.75 ± 0.05	2.849SerGlu: 2.849 ± 0.056	1.853SerPhe: 1.853 ± 0.049	5.101SerGly: 5.101 ± 0.083	1.14SerHis: 1.14 ± 0.039	2.385SerIle: 2.385 ± 0.057	1.118SerLys: 1.118 ± 0.033	5.028SerLeu: 5.028 ± 0.078	1.212SerMet: 1.212 ± 0.037	1.14SerAsn: 1.14 ± 0.034	2.461SerPro: 2.461 ± 0.051	1.337SerGln: 1.337 ± 0.042	3.926SerArg: 3.926 ± 0.069	2.452SerSer: 2.452 ± 0.061	2.296SerThr: 2.296 ± 0.049	3.898SerVal: 3.898 ± 0.069	0.633SerTrp: 0.633 ± 0.024	0.996SerTyr: 0.996 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.383ThrAla: 5.383 ± 0.079	0.428ThrCys: 0.428 ± 0.021	2.58ThrAsp: 2.58 ± 0.04	2.468ThrGlu: 2.468 ± 0.054	1.55ThrPhe: 1.55 ± 0.038	4.394ThrGly: 4.394 ± 0.074	1.17ThrHis: 1.17 ± 0.034	2.153ThrIle: 2.153 ± 0.05	0.843ThrLys: 0.843 ± 0.031	6.174ThrLeu: 6.174 ± 0.088	1.09ThrMet: 1.09 ± 0.034	1.045ThrAsn: 1.045 ± 0.036	3.132ThrPro: 3.132 ± 0.054	1.47ThrGln: 1.47 ± 0.042	3.834ThrArg: 3.834 ± 0.064	2.192ThrSer: 2.192 ± 0.057	2.431ThrThr: 2.431 ± 0.058	3.822ThrVal: 3.822 ± 0.069	0.597ThrTrp: 0.597 ± 0.027	0.933ThrTyr: 0.933 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	9.847ValAla: 9.847 ± 0.105	0.808ValCys: 0.808 ± 0.034	4.547ValAsp: 4.547 ± 0.068	5.181ValGlu: 5.181 ± 0.093	3.109ValPhe: 3.109 ± 0.06	5.741ValGly: 5.741 ± 0.088	1.707ValHis: 1.707 ± 0.036	3.8ValIle: 3.8 ± 0.07	1.838ValLys: 1.838 ± 0.05	8.633ValLeu: 8.633 ± 0.113	1.849ValMet: 1.849 ± 0.054	1.636ValAsn: 1.636 ± 0.044	3.865ValPro: 3.865 ± 0.063	2.014ValGln: 2.014 ± 0.046	6.146ValArg: 6.146 ± 0.094	4.188ValSer: 4.188 ± 0.074	4.029ValThr: 4.029 ± 0.075	6.639ValVal: 6.639 ± 0.104	0.992ValTrp: 0.992 ± 0.038	1.387ValTyr: 1.387 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.266TrpAla: 1.266 ± 0.039	0.17TrpCys: 0.17 ± 0.015	0.601TrpAsp: 0.601 ± 0.026	0.624TrpGlu: 0.624 ± 0.026	0.575TrpPhe: 0.575 ± 0.029	0.858TrpGly: 0.858 ± 0.032	0.431TrpHis: 0.431 ± 0.022	0.737TrpIle: 0.737 ± 0.031	0.371TrpLys: 0.371 ± 0.021	1.959TrpLeu: 1.959 ± 0.05	0.41TrpMet: 0.41 ± 0.021	0.382TrpAsn: 0.382 ± 0.022	0.666TrpPro: 0.666 ± 0.025	0.581TrpGln: 0.581 ± 0.022	1.37TrpArg: 1.37 ± 0.04	0.776TrpSer: 0.776 ± 0.028	0.598TrpThr: 0.598 ± 0.028	0.995TrpVal: 0.995 ± 0.039	0.28TrpTrp: 0.28 ± 0.018	0.282TrpTyr: 0.282 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.568TyrAla: 2.568 ± 0.049	0.266TyrCys: 0.266 ± 0.018	1.235TyrAsp: 1.235 ± 0.036	1.1TyrGlu: 1.1 ± 0.032	0.898TyrPhe: 0.898 ± 0.029	1.795TyrGly: 1.795 ± 0.044	0.49TyrHis: 0.49 ± 0.025	0.753TyrIle: 0.753 ± 0.03	0.512TyrLys: 0.512 ± 0.023	2.271TyrLeu: 2.271 ± 0.053	0.409TyrMet: 0.409 ± 0.018	0.553TyrAsn: 0.553 ± 0.024	1.098TyrPro: 1.098 ± 0.036	0.623TyrGln: 0.623 ± 0.028	1.956TyrArg: 1.956 ± 0.054	1.038TyrSer: 1.038 ± 0.039	1.044TyrThr: 1.044 ± 0.034	1.724TyrVal: 1.724 ± 0.042	0.347TyrTrp: 0.347 ± 0.017	0.59TyrTyr: 0.59 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3041 proteins (971649 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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