Escherichia phage flopper

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Cleopatravirinae; Carltongylesvirus; Escherichia virus flopper

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WWK7|A0A6B9WWK7_9CAUD Uncharacterized protein OS=Escherichia phage flopper OX=2696397 GN=flopper_12 PE=4 SV=1
MM1 pKa = 7.18QNDD4 pKa = 4.4NITQAVEE11 pKa = 3.82QLKK14 pKa = 9.83SWRR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 2.9EE20 pKa = 4.16TLLVRR25 pKa = 11.84FYY27 pKa = 10.62TGEE30 pKa = 4.16TNSEE34 pKa = 4.54FVDD37 pKa = 3.51AWIDD41 pKa = 3.66GLSWRR46 pKa = 11.84HH47 pKa = 5.42YY48 pKa = 10.64DD49 pKa = 3.78FDD51 pKa = 3.65MRR53 pKa = 11.84DD54 pKa = 3.79YY55 pKa = 11.33LHH57 pKa = 7.07EE58 pKa = 4.74EE59 pKa = 3.94YY60 pKa = 10.72GIEE63 pKa = 4.07YY64 pKa = 9.81PEE66 pKa = 5.34DD67 pKa = 3.26GTYY70 pKa = 11.02DD71 pKa = 3.49VIEE74 pKa = 4.57SNEE77 pKa = 3.72EE78 pKa = 3.88DD79 pKa = 3.53VTEE82 pKa = 4.09VCFANGLFSIRR93 pKa = 11.84KK94 pKa = 7.3YY95 pKa = 11.46DD96 pKa = 3.41KK97 pKa = 10.92FNEE100 pKa = 4.17LLVDD104 pKa = 3.63TDD106 pKa = 4.55LDD108 pKa = 4.01VILAAFRR115 pKa = 11.84SGDD118 pKa = 3.06IDD120 pKa = 3.72IFTVDD125 pKa = 4.29DD126 pKa = 4.76LNDD129 pKa = 3.63KK130 pKa = 10.81VIARR134 pKa = 11.84CDD136 pKa = 3.57SIADD140 pKa = 3.5YY141 pKa = 10.45FRR143 pKa = 11.84QYY145 pKa = 11.6DD146 pKa = 4.18GIEE149 pKa = 4.37VPDD152 pKa = 4.04HH153 pKa = 5.92LRR155 pKa = 11.84PYY157 pKa = 10.2IDD159 pKa = 3.26WDD161 pKa = 3.56AVARR165 pKa = 11.84DD166 pKa = 3.78YY167 pKa = 11.83GSDD170 pKa = 3.43YY171 pKa = 10.74TFSEE175 pKa = 5.41GILFSNAA182 pKa = 2.85

Molecular weight: 21.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9WSV8|A0A6B9WSV8_9CAUD Baseplate assembly protein OS=Escherichia phage flopper OX=2696397 GN=flopper_35 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84KK3 pKa = 9.56VKK5 pKa = 9.57TKK7 pKa = 9.42WYY9 pKa = 9.24MVGQLYY15 pKa = 10.79LDD17 pKa = 3.86RR18 pKa = 11.84NSFMVGQLYY27 pKa = 10.23LAEE30 pKa = 4.48ICSNRR35 pKa = 11.84SPTMKK40 pKa = 10.55

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

15211

40

1238

211.3

23.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.402 ± 0.403

1.078 ± 0.151

5.779 ± 0.284

6.587 ± 0.318

3.964 ± 0.183

7.343 ± 0.409

1.907 ± 0.151

6.075 ± 0.239

6.035 ± 0.339

7.751 ± 0.345

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.199

5.036 ± 0.2

3.833 ± 0.151

3.826 ± 0.226

4.687 ± 0.252

6.725 ± 0.344

6.272 ± 0.372

6.969 ± 0.342

1.387 ± 0.125

3.59 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski