Amino acid dipepetide frequency for Synechococcus lacustris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.495AlaAla: 13.495 ± 0.196
1.306AlaCys: 1.306 ± 0.043
5.37AlaAsp: 5.37 ± 0.096
6.617AlaGlu: 6.617 ± 0.095
3.205AlaPhe: 3.205 ± 0.071
8.456AlaGly: 8.456 ± 0.12
1.676AlaHis: 1.676 ± 0.05
5.715AlaIle: 5.715 ± 0.088
3.743AlaLys: 3.743 ± 0.076
13.642AlaLeu: 13.642 ± 0.171
2.52AlaMet: 2.52 ± 0.069
3.657AlaAsn: 3.657 ± 0.1
4.631AlaPro: 4.631 ± 0.087
4.293AlaGln: 4.293 ± 0.086
5.331AlaArg: 5.331 ± 0.105
6.569AlaSer: 6.569 ± 0.124
4.743AlaThr: 4.743 ± 0.116
7.094AlaVal: 7.094 ± 0.102
1.606AlaTrp: 1.606 ± 0.049
2.061AlaTyr: 2.061 ± 0.063
0.548AlaXaa: 0.548 ± 0.032
Cys
0.923CysAla: 0.923 ± 0.035
0.278CysCys: 0.278 ± 0.019
0.606CysAsp: 0.606 ± 0.03
0.592CysGlu: 0.592 ± 0.03
0.501CysPhe: 0.501 ± 0.022
1.114CysGly: 1.114 ± 0.042
0.334CysHis: 0.334 ± 0.02
0.527CysIle: 0.527 ± 0.026
0.383CysLys: 0.383 ± 0.023
1.628CysLeu: 1.628 ± 0.048
0.17CysMet: 0.17 ± 0.015
0.478CysAsn: 0.478 ± 0.029
0.649CysPro: 0.649 ± 0.03
0.493CysGln: 0.493 ± 0.025
0.848CysArg: 0.848 ± 0.034
0.973CysSer: 0.973 ± 0.04
0.48CysThr: 0.48 ± 0.028
0.63CysVal: 0.63 ± 0.028
0.282CysTrp: 0.282 ± 0.02
0.285CysTyr: 0.285 ± 0.02
0.06CysXaa: 0.06 ± 0.009
Asp
4.411AspAla: 4.411 ± 0.085
0.526AspCys: 0.526 ± 0.026
1.754AspAsp: 1.754 ± 0.056
2.259AspGlu: 2.259 ± 0.072
1.66AspPhe: 1.66 ± 0.049
4.397AspGly: 4.397 ± 0.099
0.821AspHis: 0.821 ± 0.036
1.909AspIle: 1.909 ± 0.061
1.405AspLys: 1.405 ± 0.049
6.595AspLeu: 6.595 ± 0.095
0.63AspMet: 0.63 ± 0.029
1.377AspAsn: 1.377 ± 0.045
3.363AspPro: 3.363 ± 0.066
2.648AspGln: 2.648 ± 0.062
3.062AspArg: 3.062 ± 0.063
2.679AspSer: 2.679 ± 0.065
1.654AspThr: 1.654 ± 0.057
2.653AspVal: 2.653 ± 0.075
0.885AspTrp: 0.885 ± 0.036
1.121AspTyr: 1.121 ± 0.046
0.17AspXaa: 0.17 ± 0.014
Glu
6.992GluAla: 6.992 ± 0.12
0.423GluCys: 0.423 ± 0.024
1.961GluAsp: 1.961 ± 0.057
3.184GluGlu: 3.184 ± 0.085
1.495GluPhe: 1.495 ± 0.05
3.92GluGly: 3.92 ± 0.07
0.949GluHis: 0.949 ± 0.036
2.902GluIle: 2.902 ± 0.071
2.162GluLys: 2.162 ± 0.062
8.89GluLeu: 8.89 ± 0.145
1.018GluMet: 1.018 ± 0.039
1.693GluAsn: 1.693 ± 0.057
2.865GluPro: 2.865 ± 0.068
3.718GluGln: 3.718 ± 0.088
4.2GluArg: 4.2 ± 0.098
2.648GluSer: 2.648 ± 0.064
2.391GluThr: 2.391 ± 0.062
3.526GluVal: 3.526 ± 0.069
0.804GluTrp: 0.804 ± 0.036
0.875GluTyr: 0.875 ± 0.034
0.257GluXaa: 0.257 ± 0.019
Phe
3.241PheAla: 3.241 ± 0.06
0.588PheCys: 0.588 ± 0.029
1.967PheAsp: 1.967 ± 0.049
1.835PheGlu: 1.835 ± 0.057
1.168PhePhe: 1.168 ± 0.048
2.955PheGly: 2.955 ± 0.063
0.64PheHis: 0.64 ± 0.029
1.53PheIle: 1.53 ± 0.055
1.192PheLys: 1.192 ± 0.037
3.365PheLeu: 3.365 ± 0.075
0.595PheMet: 0.595 ± 0.027
1.56PheAsn: 1.56 ± 0.046
1.484PhePro: 1.484 ± 0.041
1.358PheGln: 1.358 ± 0.037
1.787PheArg: 1.787 ± 0.048
2.936PheSer: 2.936 ± 0.066
1.585PheThr: 1.585 ± 0.043
1.898PheVal: 1.898 ± 0.05
0.62PheTrp: 0.62 ± 0.033
0.846PheTyr: 0.846 ± 0.032
0.119PheXaa: 0.119 ± 0.013
Gly
7.551GlyAla: 7.551 ± 0.124
1.236GlyCys: 1.236 ± 0.044
3.856GlyAsp: 3.856 ± 0.092
4.403GlyGlu: 4.403 ± 0.078
3.444GlyPhe: 3.444 ± 0.076
6.26GlyGly: 6.26 ± 0.112
1.51GlyHis: 1.51 ± 0.046
4.339GlyIle: 4.339 ± 0.086
2.953GlyLys: 2.953 ± 0.067
10.443GlyLeu: 10.443 ± 0.148
1.733GlyMet: 1.733 ± 0.049
2.747GlyAsn: 2.747 ± 0.092
3.208GlyPro: 3.208 ± 0.064
3.136GlyGln: 3.136 ± 0.073
4.558GlyArg: 4.558 ± 0.087
5.956GlySer: 5.956 ± 0.129
3.691GlyThr: 3.691 ± 0.114
5.396GlyVal: 5.396 ± 0.093
1.693GlyTrp: 1.693 ± 0.052
1.999GlyTyr: 1.999 ± 0.057
0.361GlyXaa: 0.361 ± 0.023
His
1.33HisAla: 1.33 ± 0.048
0.379HisCys: 0.379 ± 0.022
0.717HisAsp: 0.717 ± 0.03
0.759HisGlu: 0.759 ± 0.032
0.682HisPhe: 0.682 ± 0.027
1.865HisGly: 1.865 ± 0.056
0.654HisHis: 0.654 ± 0.036
0.687HisIle: 0.687 ± 0.032
0.482HisLys: 0.482 ± 0.024
2.414HisLeu: 2.414 ± 0.058
0.244HisMet: 0.244 ± 0.019
0.64HisAsn: 0.64 ± 0.033
1.293HisPro: 1.293 ± 0.043
1.126HisGln: 1.126 ± 0.046
1.527HisArg: 1.527 ± 0.052
1.222HisSer: 1.222 ± 0.043
0.735HisThr: 0.735 ± 0.03
0.865HisVal: 0.865 ± 0.038
0.577HisTrp: 0.577 ± 0.029
0.471HisTyr: 0.471 ± 0.024
0.066HisXaa: 0.066 ± 0.009
Ile
5.449IleAla: 5.449 ± 0.083
0.671IleCys: 0.671 ± 0.03
3.046IleAsp: 3.046 ± 0.068
3.127IleGlu: 3.127 ± 0.075
1.442IlePhe: 1.442 ± 0.05
4.258IleGly: 4.258 ± 0.09
0.944IleHis: 0.944 ± 0.037
1.72IleIle: 1.72 ± 0.061
1.948IleLys: 1.948 ± 0.058
3.916IleLeu: 3.916 ± 0.081
0.456IleMet: 0.456 ± 0.028
2.365IleAsn: 2.365 ± 0.064
2.617IlePro: 2.617 ± 0.059
1.877IleGln: 1.877 ± 0.047
2.624IleArg: 2.624 ± 0.064
3.922IleSer: 3.922 ± 0.098
2.932IleThr: 2.932 ± 0.078
2.418IleVal: 2.418 ± 0.067
0.803IleTrp: 0.803 ± 0.038
1.172IleTyr: 1.172 ± 0.041
0.224IleXaa: 0.224 ± 0.019
Lys
4.109LysAla: 4.109 ± 0.084
0.259LysCys: 0.259 ± 0.02
1.456LysAsp: 1.456 ± 0.045
2.115LysGlu: 2.115 ± 0.058
0.944LysPhe: 0.944 ± 0.039
2.604LysGly: 2.604 ± 0.067
0.575LysHis: 0.575 ± 0.032
1.556LysIle: 1.556 ± 0.05
1.317LysLys: 1.317 ± 0.056
4.354LysLeu: 4.354 ± 0.094
0.487LysMet: 0.487 ± 0.022
1.104LysAsn: 1.104 ± 0.036
2.344LysPro: 2.344 ± 0.058
2.127LysGln: 2.127 ± 0.061
2.329LysArg: 2.329 ± 0.064
1.982LysSer: 1.982 ± 0.055
1.699LysThr: 1.699 ± 0.047
2.071LysVal: 2.071 ± 0.051
0.339LysTrp: 0.339 ± 0.022
0.665LysTyr: 0.665 ± 0.033
0.192LysXaa: 0.192 ± 0.016
Leu
14.619LeuAla: 14.619 ± 0.175
1.322LeuCys: 1.322 ± 0.044
6.101LeuAsp: 6.101 ± 0.1
8.63LeuGlu: 8.63 ± 0.154
3.834LeuPhe: 3.834 ± 0.092
9.653LeuGly: 9.653 ± 0.13
2.28LeuHis: 2.28 ± 0.064
6.141LeuIle: 6.141 ± 0.101
4.769LeuLys: 4.769 ± 0.087
17.768LeuLeu: 17.768 ± 0.298
2.425LeuMet: 2.425 ± 0.065
4.591LeuAsn: 4.591 ± 0.081
7.201LeuPro: 7.201 ± 0.111
7.001LeuGln: 7.001 ± 0.134
7.862LeuArg: 7.862 ± 0.13
7.787LeuSer: 7.787 ± 0.1
5.128LeuThr: 5.128 ± 0.091
8.602LeuVal: 8.602 ± 0.116
1.863LeuTrp: 1.863 ± 0.056
2.073LeuTyr: 2.073 ± 0.059
0.593LeuXaa: 0.593 ± 0.035
Met
3.032MetAla: 3.032 ± 0.08
0.104MetCys: 0.104 ± 0.012
0.691MetAsp: 0.691 ± 0.032
1.092MetGlu: 1.092 ± 0.04
0.423MetPhe: 0.423 ± 0.022
1.681MetGly: 1.681 ± 0.049
0.316MetHis: 0.316 ± 0.022
0.674MetIle: 0.674 ± 0.033
0.628MetLys: 0.628 ± 0.031
2.022MetLeu: 2.022 ± 0.061
0.245MetMet: 0.245 ± 0.018
0.623MetAsn: 0.623 ± 0.029
1.138MetPro: 1.138 ± 0.043
0.885MetGln: 0.885 ± 0.032
1.094MetArg: 1.094 ± 0.041
0.825MetSer: 0.825 ± 0.032
0.811MetThr: 0.811 ± 0.034
1.403MetVal: 1.403 ± 0.047
0.098MetTrp: 0.098 ± 0.011
0.199MetTyr: 0.199 ± 0.019
0.069MetXaa: 0.069 ± 0.009
Asn
2.983AsnAla: 2.983 ± 0.083
0.525AsnCys: 0.525 ± 0.027
1.68AsnAsp: 1.68 ± 0.053
1.472AsnGlu: 1.472 ± 0.044
1.226AsnPhe: 1.226 ± 0.044
3.016AsnGly: 3.016 ± 0.1
0.779AsnHis: 0.779 ± 0.034
1.521AsnIle: 1.521 ± 0.051
1.327AsnLys: 1.327 ± 0.044
4.388AsnLeu: 4.388 ± 0.078
0.54AsnMet: 0.54 ± 0.024
1.648AsnAsn: 1.648 ± 0.073
2.754AsnPro: 2.754 ± 0.073
2.023AsnGln: 2.023 ± 0.056
2.195AsnArg: 2.195 ± 0.053
2.743AsnSer: 2.743 ± 0.085
1.648AsnThr: 1.648 ± 0.071
1.644AsnVal: 1.644 ± 0.06
0.739AsnTrp: 0.739 ± 0.034
0.982AsnTyr: 0.982 ± 0.044
0.153AsnXaa: 0.153 ± 0.016
Pro
5.556ProAla: 5.556 ± 0.101
0.53ProCys: 0.53 ± 0.025
2.552ProAsp: 2.552 ± 0.065
3.599ProGlu: 3.599 ± 0.076
1.777ProPhe: 1.777 ± 0.047
4.327ProGly: 4.327 ± 0.08
0.957ProHis: 0.957 ± 0.035
2.55ProIle: 2.55 ± 0.055
1.735ProLys: 1.735 ± 0.045
7.146ProLeu: 7.146 ± 0.121
1.121ProMet: 1.121 ± 0.039
1.963ProAsn: 1.963 ± 0.054
2.5ProPro: 2.5 ± 0.064
2.401ProGln: 2.401 ± 0.06
2.381ProArg: 2.381 ± 0.062
3.579ProSer: 3.579 ± 0.095
2.41ProThr: 2.41 ± 0.057
3.509ProVal: 3.509 ± 0.067
1.067ProTrp: 1.067 ± 0.046
1.172ProTyr: 1.172 ± 0.042
0.329ProXaa: 0.329 ± 0.024
Gln
5.426GlnAla: 5.426 ± 0.11
0.412GlnCys: 0.412 ± 0.023
1.38GlnAsp: 1.38 ± 0.039
2.772GlnGlu: 2.772 ± 0.071
1.297GlnPhe: 1.297 ± 0.04
3.559GlnGly: 3.559 ± 0.075
0.936GlnHis: 0.936 ± 0.043
2.312GlnIle: 2.312 ± 0.054
1.704GlnLys: 1.704 ± 0.042
8.318GlnLeu: 8.318 ± 0.147
0.808GlnMet: 0.808 ± 0.037
1.371GlnAsn: 1.371 ± 0.051
2.565GlnPro: 2.565 ± 0.062
3.876GlnGln: 3.876 ± 0.102
3.824GlnArg: 3.824 ± 0.084
2.365GlnSer: 2.365 ± 0.06
1.733GlnThr: 1.733 ± 0.051
3.43GlnVal: 3.43 ± 0.064
0.979GlnTrp: 0.979 ± 0.037
0.647GlnTyr: 0.647 ± 0.03
0.279GlnXaa: 0.279 ± 0.019
Arg
4.849ArgAla: 4.849 ± 0.082
0.924ArgCys: 0.924 ± 0.037
2.523ArgAsp: 2.523 ± 0.056
3.206ArgGlu: 3.206 ± 0.094
2.544ArgPhe: 2.544 ± 0.06
3.914ArgGly: 3.914 ± 0.084
1.431ArgHis: 1.431 ± 0.048
2.999ArgIle: 2.999 ± 0.064
1.882ArgLys: 1.882 ± 0.057
8.758ArgLeu: 8.758 ± 0.159
1.172ArgMet: 1.172 ± 0.039
1.959ArgAsn: 1.959 ± 0.058
2.882ArgPro: 2.882 ± 0.07
3.496ArgGln: 3.496 ± 0.085
4.55ArgArg: 4.55 ± 0.1
4.15ArgSer: 4.15 ± 0.086
2.222ArgThr: 2.222 ± 0.057
3.547ArgVal: 3.547 ± 0.075
1.514ArgTrp: 1.514 ± 0.047
1.521ArgTyr: 1.521 ± 0.049
0.239ArgXaa: 0.239 ± 0.017
Ser
6.08SerAla: 6.08 ± 0.113
0.94SerCys: 0.94 ± 0.041
3.12SerAsp: 3.12 ± 0.076
3.081SerGlu: 3.081 ± 0.063
2.559SerPhe: 2.559 ± 0.059
5.814SerGly: 5.814 ± 0.119
1.296SerHis: 1.296 ± 0.042
3.305SerIle: 3.305 ± 0.07
2.306SerLys: 2.306 ± 0.06
7.861SerLeu: 7.861 ± 0.112
1.226SerMet: 1.226 ± 0.042
2.803SerAsn: 2.803 ± 0.09
3.218SerPro: 3.218 ± 0.085
2.762SerGln: 2.762 ± 0.067
3.633SerArg: 3.633 ± 0.074
5.384SerSer: 5.384 ± 0.147
3.254SerThr: 3.254 ± 0.118
3.506SerVal: 3.506 ± 0.068
1.262SerTrp: 1.262 ± 0.042
1.688SerTyr: 1.688 ± 0.056
0.328SerXaa: 0.328 ± 0.026
Thr
5.306ThrAla: 5.306 ± 0.126
0.403ThrCys: 0.403 ± 0.023
1.962ThrAsp: 1.962 ± 0.06
2.182ThrGlu: 2.182 ± 0.055
1.385ThrPhe: 1.385 ± 0.042
3.941ThrGly: 3.941 ± 0.106
0.724ThrHis: 0.724 ± 0.031
2.302ThrIle: 2.302 ± 0.063
1.435ThrLys: 1.435 ± 0.045
4.616ThrLeu: 4.616 ± 0.086
0.724ThrMet: 0.724 ± 0.029
1.755ThrAsn: 1.755 ± 0.071
3.027ThrPro: 3.027 ± 0.081
1.388ThrGln: 1.388 ± 0.048
1.963ThrArg: 1.963 ± 0.047
3.164ThrSer: 3.164 ± 0.124
2.779ThrThr: 2.779 ± 0.116
3.07ThrVal: 3.07 ± 0.08
0.639ThrTrp: 0.639 ± 0.032
1.123ThrTyr: 1.123 ± 0.044
0.21ThrXaa: 0.21 ± 0.019
Val
7.161ValAla: 7.161 ± 0.093
0.731ValCys: 0.731 ± 0.035
3.452ValAsp: 3.452 ± 0.079
3.948ValGlu: 3.948 ± 0.088
2.122ValPhe: 2.122 ± 0.052
4.848ValGly: 4.848 ± 0.084
1.014ValHis: 1.014 ± 0.038
3.443ValIle: 3.443 ± 0.073
1.943ValLys: 1.943 ± 0.05
8.197ValLeu: 8.197 ± 0.103
1.229ValMet: 1.229 ± 0.042
2.13ValAsn: 2.13 ± 0.06
3.072ValPro: 3.072 ± 0.063
2.632ValGln: 2.632 ± 0.057
3.344ValArg: 3.344 ± 0.078
3.768ValSer: 3.768 ± 0.068
2.589ValThr: 2.589 ± 0.067
5.553ValVal: 5.553 ± 0.093
0.776ValTrp: 0.776 ± 0.03
1.06ValTyr: 1.06 ± 0.042
0.22ValXaa: 0.22 ± 0.016
Trp
1.381TrpAla: 1.381 ± 0.048
0.278TrpCys: 0.278 ± 0.017
0.701TrpAsp: 0.701 ± 0.029
0.731TrpGlu: 0.731 ± 0.034
0.675TrpPhe: 0.675 ± 0.034
1.243TrpGly: 1.243 ± 0.041
0.452TrpHis: 0.452 ± 0.027
0.775TrpIle: 0.775 ± 0.03
0.449TrpLys: 0.449 ± 0.029
2.917TrpLeu: 2.917 ± 0.079
0.323TrpMet: 0.323 ± 0.02
0.545TrpAsn: 0.545 ± 0.03
0.965TrpPro: 0.965 ± 0.038
1.459TrpGln: 1.459 ± 0.044
1.373TrpArg: 1.373 ± 0.044
0.944TrpSer: 0.944 ± 0.039
0.505TrpThr: 0.505 ± 0.028
0.964TrpVal: 0.964 ± 0.033
0.406TrpTrp: 0.406 ± 0.026
0.328TrpTyr: 0.328 ± 0.022
0.059TrpXaa: 0.059 ± 0.009
Tyr
1.857TyrAla: 1.857 ± 0.057
0.339TyrCys: 0.339 ± 0.021
0.894TyrAsp: 0.894 ± 0.032
0.994TyrGlu: 0.994 ± 0.033
0.722TyrPhe: 0.722 ± 0.029
2.266TyrGly: 2.266 ± 0.063
0.369TyrHis: 0.369 ± 0.021
0.804TyrIle: 0.804 ± 0.037
0.675TyrLys: 0.675 ± 0.034
2.46TyrLeu: 2.46 ± 0.068
0.307TyrMet: 0.307 ± 0.022
0.777TyrAsn: 0.777 ± 0.039
1.123TyrPro: 1.123 ± 0.041
1.042TyrGln: 1.042 ± 0.036
1.575TyrArg: 1.575 ± 0.046
1.492TyrSer: 1.492 ± 0.052
0.931TyrThr: 0.931 ± 0.043
1.232TyrVal: 1.232 ± 0.041
0.429TyrTrp: 0.429 ± 0.027
0.56TyrTyr: 0.56 ± 0.032
0.078TyrXaa: 0.078 ± 0.009
Xaa
0.527XaaAla: 0.527 ± 0.031
0.059XaaCys: 0.059 ± 0.008
0.192XaaAsp: 0.192 ± 0.018
0.274XaaGlu: 0.274 ± 0.019
0.164XaaPhe: 0.164 ± 0.015
0.381XaaGly: 0.381 ± 0.024
0.08XaaHis: 0.08 ± 0.011
0.218XaaIle: 0.218 ± 0.017
0.188XaaLys: 0.188 ± 0.017
0.546XaaLeu: 0.546 ± 0.031
0.083XaaMet: 0.083 ± 0.009
0.198XaaAsn: 0.198 ± 0.017
0.263XaaPro: 0.263 ± 0.017
0.253XaaGln: 0.253 ± 0.016
0.219XaaArg: 0.219 ± 0.018
0.337XaaSer: 0.337 ± 0.028
0.179XaaThr: 0.179 ± 0.015
0.223XaaVal: 0.223 ± 0.02
0.094XaaTrp: 0.094 ± 0.012
0.079XaaTyr: 0.079 ± 0.011
0.167XaaXaa: 0.167 ± 0.018
Statistics based on 2879 proteins (798731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski