Spironucleus salmonicida

Taxonomy: cellular organisms; Eukaryota; Metamonada; Fornicata; Diplomonadida; Hexamitidae; Hexamitinae; Spironucleus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8098 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|V6LS53|V6LS53_9EUKA 4Fe-4S ferredoxin iron-sulfur binding domain protein OS=Spironucleus salmonicida OX=348837 GN=SS50377_12391 PE=4 SV=1
MM1 pKa = 7.32SAWDD5 pKa = 4.93LITTHH10 pKa = 5.95SQQNLDD16 pKa = 3.53QMCFQPSTDD25 pKa = 3.35VYY27 pKa = 11.1RR28 pKa = 11.84EE29 pKa = 4.06DD30 pKa = 3.34QLIPMDD36 pKa = 5.3CSDD39 pKa = 3.63QQASDD44 pKa = 3.62GDD46 pKa = 3.41IDD48 pKa = 3.66QATRR52 pKa = 11.84QTSYY56 pKa = 11.42DD57 pKa = 3.73GVSLAQQLKK66 pKa = 8.22QQ67 pKa = 3.26

Molecular weight:
7.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|V6LUI8|V6LUI8_9EUKA Uncharacterized protein OS=Spironucleus salmonicida OX=348837 GN=SS50377_11555 PE=4 SV=1
RR1 pKa = 7.92QPVPPRR7 pKa = 11.84PRR9 pKa = 11.84PAGVPGRR16 pKa = 11.84RR17 pKa = 11.84GPGHH21 pKa = 6.95AVRR24 pKa = 11.84AVRR27 pKa = 11.84APGARR32 pKa = 11.84EE33 pKa = 3.84RR34 pKa = 11.84PRR36 pKa = 11.84GALPGLPAAPPSAPRR51 pKa = 11.84GPARR55 pKa = 11.84HH56 pKa = 5.81PQGVPGPCRR65 pKa = 11.84GCTRR69 pKa = 11.84LHH71 pKa = 4.98GHH73 pKa = 6.37ARR75 pKa = 11.84AARR78 pKa = 11.84ALPACRR84 pKa = 11.84PRR86 pKa = 11.84PSAPPAGVPGVRR98 pKa = 11.84ARR100 pKa = 11.84RR101 pKa = 11.84VPGASAPPARR111 pKa = 11.84PVRR114 pKa = 11.84RR115 pKa = 11.84SPRR118 pKa = 11.84RR119 pKa = 11.84HH120 pKa = 5.43RR121 pKa = 11.84RR122 pKa = 11.84AGACRR127 pKa = 11.84LRR129 pKa = 11.84PAGAGPRR136 pKa = 11.84EE137 pKa = 4.13RR138 pKa = 11.84GRR140 pKa = 11.84RR141 pKa = 11.84VPGALLLRR149 pKa = 11.84LQPPPVRR156 pKa = 11.84RR157 pKa = 11.84GPTRR161 pKa = 11.84HH162 pKa = 6.84PLRR165 pKa = 11.84LPRR168 pKa = 11.84RR169 pKa = 11.84ADD171 pKa = 3.09GRR173 pKa = 11.84PGHH176 pKa = 6.42GRR178 pKa = 11.84VQGPPPPGTPWRR190 pKa = 11.84ALRR193 pKa = 11.84VGAGGKK199 pKa = 9.56RR200 pKa = 11.84CWAPGRR206 pKa = 11.84CPRR209 pKa = 11.84WGG211 pKa = 3.21

Molecular weight:
22.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8098

0

8098

3053270

37

6144

377.0

43.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.273 ± 0.035

2.401 ± 0.039

4.998 ± 0.018

5.784 ± 0.026

5.23 ± 0.026

4.164 ± 0.031

1.69 ± 0.01

8.2 ± 0.033

6.882 ± 0.028

9.87 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.967 ± 0.011

6.01 ± 0.027

3.343 ± 0.022

8.29 ± 0.049

3.613 ± 0.023

7.478 ± 0.021

5.125 ± 0.019

5.5 ± 0.021

0.6 ± 0.008

3.581 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski