Stentor coeruleus

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Postciliodesmatophora; Heterotrichea; Heterotrichida; Stentoridae; Stentor

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1R2CIJ7|A0A1R2CIJ7_9CILI Uncharacterized protein OS=Stentor coeruleus OX=5963 GN=SteCoe_9126 PE=4 SV=1
MM1 pKa = 7.64LIISLLLQVIGSLGAIEE18 pKa = 5.02YY19 pKa = 7.38FTKK22 pKa = 10.53GQHH25 pKa = 5.83LKK27 pKa = 10.69FYY29 pKa = 10.18FPTVDD34 pKa = 2.94QVTFEE39 pKa = 4.14LWVPSANLLGFDD51 pKa = 4.44WIGIAIQCTNYY62 pKa = 9.43PRR64 pKa = 11.84NAFQADD70 pKa = 4.36YY71 pKa = 9.78YY72 pKa = 10.5IAMVDD77 pKa = 4.57DD78 pKa = 4.13VFTDD82 pKa = 3.54RR83 pKa = 11.84WADD86 pKa = 3.36GHH88 pKa = 5.95YY89 pKa = 10.84LPVLDD94 pKa = 4.69TDD96 pKa = 4.43LGGNSDD102 pKa = 3.61IVSDD106 pKa = 3.78RR107 pKa = 11.84YY108 pKa = 10.42EE109 pKa = 4.11YY110 pKa = 10.77EE111 pKa = 4.39DD112 pKa = 3.71YY113 pKa = 11.14VIYY116 pKa = 10.18TLTRR120 pKa = 11.84SLATGDD126 pKa = 4.18VYY128 pKa = 11.34DD129 pKa = 3.97QEE131 pKa = 5.38LVVDD135 pKa = 4.5TPYY138 pKa = 10.33MIQWAKK144 pKa = 10.87GNLVDD149 pKa = 5.44GEE151 pKa = 4.56IGVHH155 pKa = 5.76TMDD158 pKa = 3.14NCGFEE163 pKa = 4.24YY164 pKa = 10.8LILADD169 pKa = 5.35DD170 pKa = 4.11YY171 pKa = 11.83LDD173 pKa = 5.22RR174 pKa = 11.84NHH176 pKa = 7.63DD177 pKa = 4.11DD178 pKa = 3.55NGKK181 pKa = 9.9FGPWTDD187 pKa = 3.24QFRR190 pKa = 11.84DD191 pKa = 4.5FVRR194 pKa = 11.84KK195 pKa = 8.64EE196 pKa = 3.92DD197 pKa = 3.97KK198 pKa = 10.24EE199 pKa = 4.24ALVMWTDD206 pKa = 5.7LIDD209 pKa = 4.32EE210 pKa = 4.9DD211 pKa = 5.82DD212 pKa = 3.85EE213 pKa = 4.99LGNIEE218 pKa = 5.85DD219 pKa = 4.73IEE221 pKa = 5.35DD222 pKa = 4.08DD223 pKa = 3.79FATDD227 pKa = 3.24EE228 pKa = 5.54DD229 pKa = 4.45VVEE232 pKa = 4.81DD233 pKa = 3.75NN234 pKa = 4.81

Molecular weight:
26.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1R2BMS3|A0A1R2BMS3_9CILI Uncharacterized protein OS=Stentor coeruleus OX=5963 GN=SteCoe_22191 PE=3 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84KK3 pKa = 10.06NIGFYY8 pKa = 10.28DD9 pKa = 4.16LCRR12 pKa = 11.84LGKK15 pKa = 9.85ISGMGMNDD23 pKa = 2.84KK24 pKa = 10.96GGSEE28 pKa = 4.44DD29 pKa = 3.52FWPLVLSNVLKK40 pKa = 10.55RR41 pKa = 11.84ARR43 pKa = 11.84FRR45 pKa = 11.84ILSWIRR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 10.39EE54 pKa = 3.73MLRR57 pKa = 11.84YY58 pKa = 9.11NVNGCVEE65 pKa = 3.65IGLRR69 pKa = 11.84YY70 pKa = 9.75ARR72 pKa = 4.52

Molecular weight:
8.47 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30969

0

30969

13122840

66

5139

423.7

48.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.322 ± 0.01

1.939 ± 0.014

5.065 ± 0.01

7.659 ± 0.022

4.833 ± 0.012

4.35 ± 0.015

1.854 ± 0.006

8.44 ± 0.012

9.245 ± 0.023

9.556 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.006

6.267 ± 0.011

3.548 ± 0.012

3.667 ± 0.008

3.944 ± 0.011

8.307 ± 0.017

4.917 ± 0.013

4.898 ± 0.011

0.929 ± 0.004

3.974 ± 0.009

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski