Bovine faeces associated circular DNA virus 1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HWJ0|A0A160HWJ0_9VIRU Putative capsid protein OS=Bovine faeces associated circular DNA virus 1 OX=1843764 PE=4 SV=1
MM1 pKa = 8.05SDD3 pKa = 2.89RR4 pKa = 11.84TKK6 pKa = 11.08AKK8 pKa = 9.76RR9 pKa = 11.84WCFTINNPFNVDD21 pKa = 4.32PIIASPGAEE30 pKa = 4.7DD31 pKa = 5.73DD32 pKa = 3.69PDD34 pKa = 3.82EE35 pKa = 4.59PVVIDD40 pKa = 3.52LRR42 pKa = 11.84YY43 pKa = 9.13PFWYY47 pKa = 8.75HH48 pKa = 5.81HH49 pKa = 6.85AMLAVFKK56 pKa = 10.62VEE58 pKa = 3.94YY59 pKa = 9.55MVAQIEE65 pKa = 4.3KK66 pKa = 10.02GEE68 pKa = 4.24NGTPHH73 pKa = 5.85WQGFIIFKK81 pKa = 9.36DD82 pKa = 3.62QKK84 pKa = 10.13RR85 pKa = 11.84LQWLKK90 pKa = 11.02DD91 pKa = 3.52HH92 pKa = 7.0LDD94 pKa = 3.69KK95 pKa = 10.86RR96 pKa = 11.84AHH98 pKa = 5.89WEE100 pKa = 3.89VARR103 pKa = 11.84GTNQQASEE111 pKa = 4.33YY112 pKa = 8.85CKK114 pKa = 10.4KK115 pKa = 10.84DD116 pKa = 3.19EE117 pKa = 4.62TYY119 pKa = 10.83DD120 pKa = 3.64PSVIPADD127 pKa = 3.4MKK129 pKa = 10.35IRR131 pKa = 11.84GRR133 pKa = 11.84FEE135 pKa = 3.76WGSMPEE141 pKa = 3.88RR142 pKa = 11.84NVPKK146 pKa = 10.14KK147 pKa = 10.38DD148 pKa = 3.41EE149 pKa = 4.21RR150 pKa = 11.84LKK152 pKa = 11.1LAADD156 pKa = 3.79EE157 pKa = 5.07LDD159 pKa = 4.08TIKK162 pKa = 10.96DD163 pKa = 3.72GYY165 pKa = 10.09KK166 pKa = 9.89RR167 pKa = 11.84PAEE170 pKa = 4.18IDD172 pKa = 3.56SLTLMQCGFIPAYY185 pKa = 9.82KK186 pKa = 10.23EE187 pKa = 3.55LTADD191 pKa = 2.64ILGPYY196 pKa = 9.07RR197 pKa = 11.84PKK199 pKa = 10.82LQIITMIGPPGTGKK213 pKa = 10.4SYY215 pKa = 10.78AIQQYY220 pKa = 8.88FPNHH224 pKa = 5.6GRR226 pKa = 11.84CICGNNGVWFQNPTAPVMVFEE247 pKa = 4.71EE248 pKa = 4.71FCGQIQLQRR257 pKa = 11.84MLQYY261 pKa = 11.0LDD263 pKa = 4.6PYY265 pKa = 10.57PLALEE270 pKa = 4.65VKK272 pKa = 9.95GGMRR276 pKa = 11.84PAMYY280 pKa = 7.83EE281 pKa = 3.97TVIITSNTRR290 pKa = 11.84PDD292 pKa = 2.63GWYY295 pKa = 9.09TGDD298 pKa = 3.87QAGQPGKK305 pKa = 9.5RR306 pKa = 11.84TDD308 pKa = 3.44ALLALWDD315 pKa = 3.81RR316 pKa = 11.84LGFEE320 pKa = 4.16SGAYY324 pKa = 9.89VPVRR328 pKa = 11.84TCGHH332 pKa = 5.13YY333 pKa = 9.99WEE335 pKa = 5.04VTPGWSIEE343 pKa = 3.89EE344 pKa = 3.94ARR346 pKa = 11.84DD347 pKa = 3.56FFTLKK352 pKa = 8.77VTRR355 pKa = 11.84FLDD358 pKa = 3.12IAEE361 pKa = 5.18IIDD364 pKa = 4.43DD365 pKa = 4.43EE366 pKa = 5.33DD367 pKa = 4.52GQGHH371 pKa = 7.15PGAAAAADD379 pKa = 3.76SQEE382 pKa = 4.04EE383 pKa = 4.09HH384 pKa = 6.9RR385 pKa = 11.84SIYY388 pKa = 10.0HH389 pKa = 5.89HH390 pKa = 6.96

Molecular weight: 44.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HWJ0|A0A160HWJ0_9VIRU Putative capsid protein OS=Bovine faeces associated circular DNA virus 1 OX=1843764 PE=4 SV=1
MM1 pKa = 7.66PSGYY5 pKa = 9.71RR6 pKa = 11.84YY7 pKa = 9.42RR8 pKa = 11.84RR9 pKa = 11.84SYY11 pKa = 8.18YY12 pKa = 9.79GRR14 pKa = 11.84YY15 pKa = 8.33RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 8.91RR19 pKa = 11.84RR20 pKa = 11.84AYY22 pKa = 7.76RR23 pKa = 11.84SLYY26 pKa = 9.0RR27 pKa = 11.84RR28 pKa = 11.84YY29 pKa = 9.21GRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 9.98SYY36 pKa = 11.13ARR38 pKa = 11.84RR39 pKa = 11.84FANGSSASQVRR50 pKa = 11.84IKK52 pKa = 11.14VPVSVAYY59 pKa = 10.38AMYY62 pKa = 9.48PVTAAGSQAVCIAHH76 pKa = 7.48PFRR79 pKa = 11.84AFTSSRR85 pKa = 11.84KK86 pKa = 8.87WSPLGTKK93 pKa = 10.22LYY95 pKa = 10.03QAYY98 pKa = 10.23ANLYY102 pKa = 10.21EE103 pKa = 4.1EE104 pKa = 4.98VKK106 pKa = 10.99CIGAKK111 pKa = 9.72VQVSVCTPLGTSSIPALEE129 pKa = 5.21VYY131 pKa = 7.57TAWDD135 pKa = 3.46RR136 pKa = 11.84RR137 pKa = 11.84TGTSEE142 pKa = 3.69NGSLNSEE149 pKa = 4.37DD150 pKa = 3.36LAGYY154 pKa = 8.61GSCQQFTAVSNSVAKK169 pKa = 9.85FVRR172 pKa = 11.84SCYY175 pKa = 10.69ARR177 pKa = 11.84DD178 pKa = 3.77LMEE181 pKa = 4.28KK182 pKa = 9.94AQWHH186 pKa = 6.73DD187 pKa = 3.24SSLAEE192 pKa = 4.16STSVSDD198 pKa = 4.07EE199 pKa = 3.92AFEE202 pKa = 4.72AAGANPNFFCPGLYY216 pKa = 10.37VGFKK220 pKa = 10.14TPGLADD226 pKa = 3.63DD227 pKa = 3.98QVRR230 pKa = 11.84EE231 pKa = 3.95VDD233 pKa = 3.08IMINAKK239 pKa = 9.86YY240 pKa = 10.44YY241 pKa = 10.13YY242 pKa = 10.26VFRR245 pKa = 11.84NPGFGASAASKK256 pKa = 8.97SAVRR260 pKa = 11.84AVEE263 pKa = 3.76PVMRR267 pKa = 11.84ASAPEE272 pKa = 3.97FEE274 pKa = 5.75VEE276 pKa = 4.84DD277 pKa = 4.37LDD279 pKa = 4.84LAGDD283 pKa = 3.95EE284 pKa = 5.19DD285 pKa = 4.19VQADD289 pKa = 4.47IIAQEE294 pKa = 4.11LAAKK298 pKa = 9.99RR299 pKa = 11.84ATVKK303 pKa = 9.45KK304 pKa = 7.89TATKK308 pKa = 10.18IVTPKK313 pKa = 10.4KK314 pKa = 10.44

Molecular weight: 34.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

704

314

390

352.0

39.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.227 ± 1.891

1.847 ± 0.039

5.966 ± 1.097

5.966 ± 0.523

3.977 ± 0.093

6.96 ± 0.546

1.847 ± 0.727

4.972 ± 1.265

5.398 ± 0.182

5.54 ± 0.458

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.409

2.841 ± 0.015

5.966 ± 0.905

4.119 ± 0.561

7.102 ± 1.09

6.108 ± 2.261

4.972 ± 0.117

6.25 ± 1.219

1.989 ± 0.621

5.682 ± 0.987
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski