Porphyra umbilicalis (Purple laver) (Red alga)

Taxonomy: cellular organisms; Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Porphyra

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13469 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6PII2|A0A1X6PII2_PORUM Uncharacterized protein OS=Porphyra umbilicalis OX=2786 GN=BU14_0047s0012 PE=4 SV=1
MM1 pKa = 6.73ATAVAFPLAAAVSAAAADD19 pKa = 3.75ARR21 pKa = 11.84AAPVGRR27 pKa = 11.84QAEE30 pKa = 4.74GEE32 pKa = 4.07AAPAPEE38 pKa = 4.87GGVVPLGGPCATAADD53 pKa = 4.47CAAPTDD59 pKa = 3.98GGPPAEE65 pKa = 4.58CVAGPGGDD73 pKa = 3.43GGGDD77 pKa = 3.48GDD79 pKa = 4.86GGSDD83 pKa = 3.88GDD85 pKa = 4.14GGGDD89 pKa = 3.67GDD91 pKa = 5.13SGGDD95 pKa = 3.46GDD97 pKa = 5.51GGGDD101 pKa = 3.66GDD103 pKa = 4.46GGGDD107 pKa = 3.36GGGDD111 pKa = 3.33GDD113 pKa = 4.36SDD115 pKa = 3.82NGVGDD120 pKa = 3.79EE121 pKa = 4.27VSTCRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84HH129 pKa = 5.3AAGAPCTTAADD140 pKa = 3.68ACHH143 pKa = 6.92RR144 pKa = 11.84DD145 pKa = 3.79LLCGEE150 pKa = 5.0GGVCVPPPPEE160 pKa = 3.8EE161 pKa = 3.77AAGADD166 pKa = 3.97RR167 pKa = 11.84YY168 pKa = 10.74RR169 pKa = 11.84QFF171 pKa = 3.93

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6NRX2|A0A1X6NRX2_PORUM Uncharacterized protein OS=Porphyra umbilicalis OX=2786 GN=BU14_0553s0002 PE=4 SV=1
MM1 pKa = 6.85TWRR4 pKa = 11.84GRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84LRR10 pKa = 11.84PPPRR14 pKa = 11.84RR15 pKa = 11.84PPSRR19 pKa = 11.84GWPNSAPRR27 pKa = 11.84ACASGAPPTTLRR39 pKa = 11.84KK40 pKa = 7.65WSKK43 pKa = 9.15RR44 pKa = 11.84TRR46 pKa = 11.84TWRR49 pKa = 11.84ASRR52 pKa = 11.84RR53 pKa = 11.84GCSTKK58 pKa = 10.08RR59 pKa = 11.84RR60 pKa = 11.84ASRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84SSGGTTARR73 pKa = 11.84RR74 pKa = 11.84GGARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84ARR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84WRR87 pKa = 11.84WSRR90 pKa = 11.84RR91 pKa = 11.84APRR94 pKa = 11.84APATPLRR101 pKa = 11.84PSRR104 pKa = 11.84RR105 pKa = 11.84CARR108 pKa = 11.84GGWRR112 pKa = 11.84ARR114 pKa = 11.84PRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84WVPRR128 pKa = 11.84ARR130 pKa = 11.84RR131 pKa = 11.84KK132 pKa = 9.59RR133 pKa = 11.84ASAMAGGGRR142 pKa = 11.84AWRR145 pKa = 11.84RR146 pKa = 11.84ASALTRR152 pKa = 11.84TFPWSSSTLRR162 pKa = 11.84SSARR166 pKa = 11.84TPSLCPRR173 pKa = 11.84AARR176 pKa = 11.84GGVAAARR183 pKa = 11.84PVGRR187 pKa = 11.84RR188 pKa = 11.84GAPRR192 pKa = 11.84PATSTRR198 pKa = 11.84GRR200 pKa = 11.84TGGGGSRR207 pKa = 11.84GQAA210 pKa = 2.96

Molecular weight: 23.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13291

178

13469

5967975

29

4583

443.1

45.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

17.515 ± 0.056

1.519 ± 0.011

4.632 ± 0.021

3.541 ± 0.022

2.029 ± 0.013

11.735 ± 0.05

2.079 ± 0.01

1.832 ± 0.014

2.119 ± 0.02

7.097 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.454 ± 0.009

1.568 ± 0.011

9.17 ± 0.046

2.058 ± 0.014

9.777 ± 0.049

6.523 ± 0.028

5.393 ± 0.016

7.315 ± 0.025

1.297 ± 0.009

1.346 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski