Amino acid dipepetide frequency for Microbacterium saperdae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.208AlaAla: 20.208 ± 0.168
0.617AlaCys: 0.617 ± 0.02
8.4AlaAsp: 8.4 ± 0.102
8.282AlaGlu: 8.282 ± 0.088
4.105AlaPhe: 4.105 ± 0.057
11.808AlaGly: 11.808 ± 0.115
2.54AlaHis: 2.54 ± 0.049
6.39AlaIle: 6.39 ± 0.078
2.524AlaLys: 2.524 ± 0.055
14.547AlaLeu: 14.547 ± 0.128
2.599AlaMet: 2.599 ± 0.044
2.273AlaAsn: 2.273 ± 0.046
6.542AlaPro: 6.542 ± 0.107
4.064AlaGln: 4.064 ± 0.053
8.9AlaArg: 8.9 ± 0.104
7.395AlaSer: 7.395 ± 0.069
7.435AlaThr: 7.435 ± 0.084
11.646AlaVal: 11.646 ± 0.125
1.884AlaTrp: 1.884 ± 0.043
2.405AlaTyr: 2.405 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.638CysAla: 0.638 ± 0.021
0.045CysCys: 0.045 ± 0.006
0.298CysAsp: 0.298 ± 0.015
0.232CysGlu: 0.232 ± 0.013
0.168CysPhe: 0.168 ± 0.012
0.5CysGly: 0.5 ± 0.018
0.105CysHis: 0.105 ± 0.009
0.197CysIle: 0.197 ± 0.012
0.043CysLys: 0.043 ± 0.006
0.399CysLeu: 0.399 ± 0.016
0.076CysMet: 0.076 ± 0.009
0.087CysAsn: 0.087 ± 0.008
0.235CysPro: 0.235 ± 0.015
0.102CysGln: 0.102 ± 0.008
0.276CysArg: 0.276 ± 0.015
0.3CysSer: 0.3 ± 0.017
0.323CysThr: 0.323 ± 0.015
0.376CysVal: 0.376 ± 0.017
0.061CysTrp: 0.061 ± 0.006
0.086CysTyr: 0.086 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
9.465AspAla: 9.465 ± 0.099
0.201AspCys: 0.201 ± 0.012
4.416AspAsp: 4.416 ± 0.078
4.298AspGlu: 4.298 ± 0.071
1.75AspPhe: 1.75 ± 0.039
6.256AspGly: 6.256 ± 0.087
1.137AspHis: 1.137 ± 0.031
2.724AspIle: 2.724 ± 0.051
0.958AspLys: 0.958 ± 0.032
6.168AspLeu: 6.168 ± 0.071
0.833AspMet: 0.833 ± 0.025
0.964AspAsn: 0.964 ± 0.031
4.079AspPro: 4.079 ± 0.07
1.521AspGln: 1.521 ± 0.039
4.342AspArg: 4.342 ± 0.055
2.714AspSer: 2.714 ± 0.052
3.006AspThr: 3.006 ± 0.055
5.524AspVal: 5.524 ± 0.068
0.988AspTrp: 0.988 ± 0.027
1.224AspTyr: 1.224 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.759GluAla: 6.759 ± 0.074
0.237GluCys: 0.237 ± 0.014
2.82GluAsp: 2.82 ± 0.055
3.154GluGlu: 3.154 ± 0.058
1.718GluPhe: 1.718 ± 0.039
4.1GluGly: 4.1 ± 0.061
1.405GluHis: 1.405 ± 0.033
2.954GluIle: 2.954 ± 0.054
1.482GluLys: 1.482 ± 0.039
6.243GluLeu: 6.243 ± 0.084
0.979GluMet: 0.979 ± 0.025
1.35GluAsn: 1.35 ± 0.033
2.853GluPro: 2.853 ± 0.052
2.178GluGln: 2.178 ± 0.047
4.936GluArg: 4.936 ± 0.076
3.101GluSer: 3.101 ± 0.049
3.138GluThr: 3.138 ± 0.049
4.531GluVal: 4.531 ± 0.058
0.886GluTrp: 0.886 ± 0.025
1.206GluTyr: 1.206 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.335PheAla: 4.335 ± 0.062
0.168PheCys: 0.168 ± 0.013
2.428PheAsp: 2.428 ± 0.049
1.685PheGlu: 1.685 ± 0.037
1.098PhePhe: 1.098 ± 0.033
3.374PheGly: 3.374 ± 0.056
0.556PheHis: 0.556 ± 0.023
1.279PheIle: 1.279 ± 0.033
0.4PheLys: 0.4 ± 0.015
3.025PheLeu: 3.025 ± 0.056
0.431PheMet: 0.431 ± 0.016
0.657PheAsn: 0.657 ± 0.022
1.461PhePro: 1.461 ± 0.034
0.827PheGln: 0.827 ± 0.025
1.925PheArg: 1.925 ± 0.04
1.919PheSer: 1.919 ± 0.035
2.262PheThr: 2.262 ± 0.049
2.851PheVal: 2.851 ± 0.054
0.513PheTrp: 0.513 ± 0.018
0.616PheTyr: 0.616 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.92GlyAla: 10.92 ± 0.104
0.515GlyCys: 0.515 ± 0.02
5.077GlyAsp: 5.077 ± 0.071
4.91GlyGlu: 4.91 ± 0.056
3.267GlyPhe: 3.267 ± 0.053
7.571GlyGly: 7.571 ± 0.115
1.707GlyHis: 1.707 ± 0.034
5.192GlyIle: 5.192 ± 0.07
1.97GlyLys: 1.97 ± 0.052
8.615GlyLeu: 8.615 ± 0.108
1.944GlyMet: 1.944 ± 0.042
1.688GlyAsn: 1.688 ± 0.045
3.593GlyPro: 3.593 ± 0.053
2.361GlyGln: 2.361 ± 0.044
5.958GlyArg: 5.958 ± 0.075
5.342GlySer: 5.342 ± 0.073
5.731GlyThr: 5.731 ± 0.091
8.109GlyVal: 8.109 ± 0.076
1.727GlyTrp: 1.727 ± 0.044
2.254GlyTyr: 2.254 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.443HisAla: 2.443 ± 0.049
0.089HisCys: 0.089 ± 0.008
1.344HisAsp: 1.344 ± 0.033
1.162HisGlu: 1.162 ± 0.033
0.553HisPhe: 0.553 ± 0.021
1.995HisGly: 1.995 ± 0.044
0.555HisHis: 0.555 ± 0.026
0.779HisIle: 0.779 ± 0.023
0.237HisLys: 0.237 ± 0.014
2.036HisLeu: 2.036 ± 0.045
0.309HisMet: 0.309 ± 0.014
0.343HisAsn: 0.343 ± 0.017
1.434HisPro: 1.434 ± 0.031
0.497HisGln: 0.497 ± 0.017
1.57HisArg: 1.57 ± 0.034
0.948HisSer: 0.948 ± 0.024
1.036HisThr: 1.036 ± 0.031
1.586HisVal: 1.586 ± 0.035
0.291HisTrp: 0.291 ± 0.014
0.409HisTyr: 0.409 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
7.818IleAla: 7.818 ± 0.08
0.246IleCys: 0.246 ± 0.014
3.783IleAsp: 3.783 ± 0.058
2.956IleGlu: 2.956 ± 0.059
1.3IlePhe: 1.3 ± 0.036
5.119IleGly: 5.119 ± 0.068
0.71IleHis: 0.71 ± 0.022
2.145IleIle: 2.145 ± 0.052
0.697IleLys: 0.697 ± 0.024
4.127IleLeu: 4.127 ± 0.062
0.673IleMet: 0.673 ± 0.025
0.91IleAsn: 0.91 ± 0.028
2.701IlePro: 2.701 ± 0.048
1.027IleGln: 1.027 ± 0.029
3.013IleArg: 3.013 ± 0.051
2.674IleSer: 2.674 ± 0.042
3.176IleThr: 3.176 ± 0.056
5.092IleVal: 5.092 ± 0.067
0.578IleTrp: 0.578 ± 0.021
0.78IleTyr: 0.78 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
2.258LysAla: 2.258 ± 0.05
0.054LysCys: 0.054 ± 0.006
1.134LysAsp: 1.134 ± 0.032
0.946LysGlu: 0.946 ± 0.028
0.464LysPhe: 0.464 ± 0.018
1.463LysGly: 1.463 ± 0.038
0.397LysHis: 0.397 ± 0.02
0.916LysIle: 0.916 ± 0.025
0.719LysLys: 0.719 ± 0.029
1.698LysLeu: 1.698 ± 0.038
0.356LysMet: 0.356 ± 0.017
0.507LysAsn: 0.507 ± 0.022
1.081LysPro: 1.081 ± 0.034
0.631LysGln: 0.631 ± 0.023
1.304LysArg: 1.304 ± 0.041
1.112LysSer: 1.112 ± 0.029
1.241LysThr: 1.241 ± 0.035
1.542LysVal: 1.542 ± 0.038
0.244LysTrp: 0.244 ± 0.013
0.401LysTyr: 0.401 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.246LeuAla: 14.246 ± 0.121
0.478LeuCys: 0.478 ± 0.018
6.652LeuAsp: 6.652 ± 0.082
4.875LeuGlu: 4.875 ± 0.068
3.069LeuPhe: 3.069 ± 0.055
9.131LeuGly: 9.131 ± 0.088
1.934LeuHis: 1.934 ± 0.04
5.092LeuIle: 5.092 ± 0.076
1.559LeuLys: 1.559 ± 0.038
10.535LeuLeu: 10.535 ± 0.135
1.661LeuMet: 1.661 ± 0.045
1.806LeuAsn: 1.806 ± 0.041
5.527LeuPro: 5.527 ± 0.08
2.705LeuGln: 2.705 ± 0.049
7.664LeuArg: 7.664 ± 0.092
6.353LeuSer: 6.353 ± 0.074
6.541LeuThr: 6.541 ± 0.073
9.054LeuVal: 9.054 ± 0.102
1.341LeuTrp: 1.341 ± 0.032
1.636LeuTyr: 1.636 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
1.959MetAla: 1.959 ± 0.036
0.076MetCys: 0.076 ± 0.008
0.779MetAsp: 0.779 ± 0.026
0.622MetGlu: 0.622 ± 0.022
0.53MetPhe: 0.53 ± 0.02
1.197MetGly: 1.197 ± 0.03
0.34MetHis: 0.34 ± 0.016
0.996MetIle: 0.996 ± 0.031
0.439MetLys: 0.439 ± 0.02
2.04MetLeu: 2.04 ± 0.044
0.363MetMet: 0.363 ± 0.019
0.529MetAsn: 0.529 ± 0.02
1.159MetPro: 1.159 ± 0.03
0.554MetGln: 0.554 ± 0.02
1.372MetArg: 1.372 ± 0.034
1.547MetSer: 1.547 ± 0.036
1.799MetThr: 1.799 ± 0.042
1.309MetVal: 1.309 ± 0.027
0.2MetTrp: 0.2 ± 0.013
0.276MetTyr: 0.276 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.486AsnAla: 2.486 ± 0.048
0.093AsnCys: 0.093 ± 0.008
1.207AsnAsp: 1.207 ± 0.032
0.953AsnGlu: 0.953 ± 0.031
0.652AsnPhe: 0.652 ± 0.028
1.915AsnGly: 1.915 ± 0.051
0.366AsnHis: 0.366 ± 0.016
0.875AsnIle: 0.875 ± 0.026
0.362AsnLys: 0.362 ± 0.017
1.855AsnLeu: 1.855 ± 0.043
0.308AsnMet: 0.308 ± 0.015
0.458AsnAsn: 0.458 ± 0.019
1.607AsnPro: 1.607 ± 0.036
0.536AsnGln: 0.536 ± 0.023
1.25AsnArg: 1.25 ± 0.039
1.012AsnSer: 1.012 ± 0.032
1.182AsnThr: 1.182 ± 0.029
1.601AsnVal: 1.601 ± 0.037
0.307AsnTrp: 0.307 ± 0.015
0.431AsnTyr: 0.431 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
6.99ProAla: 6.99 ± 0.095
0.15ProCys: 0.15 ± 0.011
3.687ProAsp: 3.687 ± 0.055
3.828ProGlu: 3.828 ± 0.068
1.743ProPhe: 1.743 ± 0.035
4.702ProGly: 4.702 ± 0.06
1.132ProHis: 1.132 ± 0.029
2.196ProIle: 2.196 ± 0.044
0.938ProLys: 0.938 ± 0.033
5.137ProLeu: 5.137 ± 0.068
0.855ProMet: 0.855 ± 0.028
1.013ProAsn: 1.013 ± 0.026
2.164ProPro: 2.164 ± 0.055
1.582ProGln: 1.582 ± 0.037
3.295ProArg: 3.295 ± 0.051
3.232ProSer: 3.232 ± 0.051
3.411ProThr: 3.411 ± 0.058
4.763ProVal: 4.763 ± 0.062
0.869ProTrp: 0.869 ± 0.028
1.075ProTyr: 1.075 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.55GlnAla: 3.55 ± 0.063
0.086GlnCys: 0.086 ± 0.008
1.401GlnAsp: 1.401 ± 0.035
1.428GlnGlu: 1.428 ± 0.037
0.929GlnPhe: 0.929 ± 0.025
2.197GlnGly: 2.197 ± 0.045
0.635GlnHis: 0.635 ± 0.024
1.513GlnIle: 1.513 ± 0.034
0.642GlnLys: 0.642 ± 0.023
3.11GlnLeu: 3.11 ± 0.051
0.522GlnMet: 0.522 ± 0.019
0.69GlnAsn: 0.69 ± 0.024
1.382GlnPro: 1.382 ± 0.03
1.14GlnGln: 1.14 ± 0.035
2.392GlnArg: 2.392 ± 0.047
1.53GlnSer: 1.53 ± 0.028
1.633GlnThr: 1.633 ± 0.033
2.338GlnVal: 2.338 ± 0.04
0.451GlnTrp: 0.451 ± 0.017
0.591GlnTyr: 0.591 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
8.796ArgAla: 8.796 ± 0.1
0.29ArgCys: 0.29 ± 0.015
4.234ArgAsp: 4.234 ± 0.065
4.236ArgGlu: 4.236 ± 0.066
2.306ArgPhe: 2.306 ± 0.048
5.381ArgGly: 5.381 ± 0.074
1.505ArgHis: 1.505 ± 0.035
3.871ArgIle: 3.871 ± 0.053
1.265ArgLys: 1.265 ± 0.037
7.05ArgLeu: 7.05 ± 0.086
1.804ArgMet: 1.804 ± 0.038
1.303ArgAsn: 1.303 ± 0.035
3.405ArgPro: 3.405 ± 0.061
2.048ArgGln: 2.048 ± 0.042
6.447ArgArg: 6.447 ± 0.085
4.124ArgSer: 4.124 ± 0.061
4.505ArgThr: 4.505 ± 0.061
5.848ArgVal: 5.848 ± 0.061
1.167ArgTrp: 1.167 ± 0.033
1.453ArgTyr: 1.453 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
7.617SerAla: 7.617 ± 0.078
0.277SerCys: 0.277 ± 0.016
3.444SerAsp: 3.444 ± 0.053
2.922SerGlu: 2.922 ± 0.049
2.012SerPhe: 2.012 ± 0.042
5.942SerGly: 5.942 ± 0.076
1.062SerHis: 1.062 ± 0.029
2.871SerIle: 2.871 ± 0.049
1.026SerLys: 1.026 ± 0.03
5.543SerLeu: 5.543 ± 0.073
1.209SerMet: 1.209 ± 0.027
1.064SerAsn: 1.064 ± 0.028
3.066SerPro: 3.066 ± 0.044
1.397SerGln: 1.397 ± 0.033
3.857SerArg: 3.857 ± 0.064
3.679SerSer: 3.679 ± 0.068
3.824SerThr: 3.824 ± 0.055
4.907SerVal: 4.907 ± 0.068
0.964SerTrp: 0.964 ± 0.028
1.165SerTyr: 1.165 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
8.468ThrAla: 8.468 ± 0.101
0.262ThrCys: 0.262 ± 0.014
3.667ThrAsp: 3.667 ± 0.057
3.08ThrGlu: 3.08 ± 0.05
1.978ThrPhe: 1.978 ± 0.04
5.826ThrGly: 5.826 ± 0.083
1.228ThrHis: 1.228 ± 0.031
3.145ThrIle: 3.145 ± 0.052
1.143ThrLys: 1.143 ± 0.032
6.357ThrLeu: 6.357 ± 0.066
1.002ThrMet: 1.002 ± 0.027
1.098ThrAsn: 1.098 ± 0.029
3.981ThrPro: 3.981 ± 0.057
1.548ThrGln: 1.548 ± 0.035
3.796ThrArg: 3.796 ± 0.053
3.488ThrSer: 3.488 ± 0.045
4.103ThrThr: 4.103 ± 0.063
5.933ThrVal: 5.933 ± 0.076
0.943ThrTrp: 0.943 ± 0.026
1.215ThrTyr: 1.215 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
11.561ValAla: 11.561 ± 0.105
0.46ValCys: 0.46 ± 0.018
5.663ValAsp: 5.663 ± 0.071
4.716ValGlu: 4.716 ± 0.062
2.881ValPhe: 2.881 ± 0.055
6.957ValGly: 6.957 ± 0.087
1.734ValHis: 1.734 ± 0.039
4.795ValIle: 4.795 ± 0.057
1.465ValLys: 1.465 ± 0.036
9.627ValLeu: 9.627 ± 0.09
1.523ValMet: 1.523 ± 0.039
1.747ValAsn: 1.747 ± 0.036
4.697ValPro: 4.697 ± 0.059
2.327ValGln: 2.327 ± 0.042
5.977ValArg: 5.977 ± 0.08
5.239ValSer: 5.239 ± 0.069
5.714ValThr: 5.714 ± 0.078
8.449ValVal: 8.449 ± 0.09
1.194ValTrp: 1.194 ± 0.031
1.477ValTyr: 1.477 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.648TrpAla: 1.648 ± 0.039
0.097TrpCys: 0.097 ± 0.009
0.833TrpAsp: 0.833 ± 0.024
0.706TrpGlu: 0.706 ± 0.025
0.581TrpPhe: 0.581 ± 0.021
1.185TrpGly: 1.185 ± 0.032
0.309TrpHis: 0.309 ± 0.016
0.824TrpIle: 0.824 ± 0.024
0.29TrpLys: 0.29 ± 0.017
1.673TrpLeu: 1.673 ± 0.041
0.36TrpMet: 0.36 ± 0.015
0.487TrpAsn: 0.487 ± 0.016
0.713TrpPro: 0.713 ± 0.026
0.56TrpGln: 0.56 ± 0.02
1.203TrpArg: 1.203 ± 0.034
0.993TrpSer: 0.993 ± 0.031
0.986TrpThr: 0.986 ± 0.03
1.19TrpVal: 1.19 ± 0.034
0.362TrpTrp: 0.362 ± 0.018
0.29TrpTyr: 0.29 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.401TyrAla: 2.401 ± 0.042
0.106TyrCys: 0.106 ± 0.009
1.298TyrAsp: 1.298 ± 0.028
1.041TyrGlu: 1.041 ± 0.026
0.704TyrPhe: 0.704 ± 0.026
1.828TyrGly: 1.828 ± 0.035
0.257TyrHis: 0.257 ± 0.015
0.771TyrIle: 0.771 ± 0.024
0.312TyrLys: 0.312 ± 0.015
2.077TyrLeu: 2.077 ± 0.032
0.276TyrMet: 0.276 ± 0.015
0.482TyrAsn: 0.482 ± 0.025
1.086TyrPro: 1.086 ± 0.033
0.556TyrGln: 0.556 ± 0.02
1.561TyrArg: 1.561 ± 0.032
1.143TyrSer: 1.143 ± 0.03
1.214TyrThr: 1.214 ± 0.039
1.558TyrVal: 1.558 ± 0.038
0.32TyrTrp: 0.32 ± 0.015
0.462TyrTyr: 0.462 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4035 proteins (1318807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski