Epulopiscium sp. Nuni2H_MBin003

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2YKY1|A0A1V2YKY1_9FIRM G5 domain-containing protein OS=Epulopiscium sp. Nuni2H_MBin003 OX=1884657 GN=BEN19_06205 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.13KK3 pKa = 10.66CMMVLYY9 pKa = 10.34LLIGCLFTNIYY20 pKa = 8.8ATEE23 pKa = 4.03DD24 pKa = 3.68MEE26 pKa = 4.36NGRR29 pKa = 11.84YY30 pKa = 7.58QVYY33 pKa = 11.02NNTLYY38 pKa = 10.98EE39 pKa = 4.17NAVGIAMSRR48 pKa = 11.84SYY50 pKa = 10.78TEE52 pKa = 4.86EE53 pKa = 3.13ITEE56 pKa = 4.39IIKK59 pKa = 9.88MDD61 pKa = 3.89DD62 pKa = 3.14KK63 pKa = 11.6LYY65 pKa = 11.41VEE67 pKa = 4.84VSLNNTQYY75 pKa = 10.53MGDD78 pKa = 3.46FTITVNGITVPNEE91 pKa = 4.05TIFHH95 pKa = 6.87DD96 pKa = 4.19EE97 pKa = 3.87EE98 pKa = 4.48SVANMKK104 pKa = 8.61TVQFEE109 pKa = 4.78VNSLTDD115 pKa = 4.72DD116 pKa = 3.63ITVGMYY122 pKa = 9.49VGPMGRR128 pKa = 11.84DD129 pKa = 3.11VEE131 pKa = 4.53YY132 pKa = 11.05GLEE135 pKa = 4.31FYY137 pKa = 11.05LDD139 pKa = 3.43TLTLVEE145 pKa = 5.58EE146 pKa = 4.23LDD148 pKa = 3.77EE149 pKa = 4.4YY150 pKa = 11.53NIGVANLEE158 pKa = 4.46DD159 pKa = 3.52IEE161 pKa = 4.53MEE163 pKa = 4.11VEE165 pKa = 4.01EE166 pKa = 5.04VIDD169 pKa = 4.73DD170 pKa = 4.05VVDD173 pKa = 4.04DD174 pKa = 5.02VIDD177 pKa = 4.24DD178 pKa = 4.03VVDD181 pKa = 3.99DD182 pKa = 4.29VVDD185 pKa = 4.1DD186 pKa = 4.24VVDD189 pKa = 4.51DD190 pKa = 4.07VASEE194 pKa = 4.22VIEE197 pKa = 4.08PVVEE201 pKa = 4.78SIADD205 pKa = 3.71VVEE208 pKa = 4.17VADD211 pKa = 5.68DD212 pKa = 3.58VSKK215 pKa = 11.58NNNHH219 pKa = 6.16TFTIVGIIAVIILILAFIIGGKK241 pKa = 10.1KK242 pKa = 9.6NGKK245 pKa = 8.32NAA247 pKa = 3.54

Molecular weight: 27.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2YHX0|A0A1V2YHX0_9FIRM Oxaloacetate decarboxylase gamma chain OS=Epulopiscium sp. Nuni2H_MBin003 OX=1884657 GN=BEN19_08160 PE=3 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.95GHH5 pKa = 6.56RR6 pKa = 11.84SQIKK10 pKa = 9.55RR11 pKa = 11.84EE12 pKa = 3.86RR13 pKa = 11.84NRR15 pKa = 11.84TNVEE19 pKa = 3.71QRR21 pKa = 11.84PHH23 pKa = 5.69AKK25 pKa = 9.8ARR27 pKa = 11.84YY28 pKa = 8.53VRR30 pKa = 11.84LSSSKK35 pKa = 11.02AKK37 pKa = 10.19IVLDD41 pKa = 3.77QIKK44 pKa = 10.59GKK46 pKa = 10.22DD47 pKa = 3.25VSTAIGILRR56 pKa = 11.84YY57 pKa = 7.09TPRR60 pKa = 11.84YY61 pKa = 8.37AADD64 pKa = 3.61VIMKK68 pKa = 9.71IVNSAVANAEE78 pKa = 4.3HH79 pKa = 6.92NNGLDD84 pKa = 3.32TTNLYY89 pKa = 9.5VQEE92 pKa = 4.86AYY94 pKa = 8.62ATQGPTLKK102 pKa = 10.35RR103 pKa = 11.84IRR105 pKa = 11.84PRR107 pKa = 11.84AQGRR111 pKa = 11.84AFRR114 pKa = 11.84IEE116 pKa = 4.51KK117 pKa = 8.09KK118 pKa = 7.6TCHH121 pKa = 5.21ITVVLNEE128 pKa = 4.04RR129 pKa = 3.9

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1656

0

1656

525598

37

1676

317.4

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.32 ± 0.058

1.161 ± 0.021

5.795 ± 0.047

6.833 ± 0.057

4.027 ± 0.042

6.13 ± 0.055

1.608 ± 0.023

10.143 ± 0.065

7.329 ± 0.056

9.056 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.758 ± 0.03

5.805 ± 0.041

3.024 ± 0.033

3.301 ± 0.035

3.165 ± 0.038

5.99 ± 0.049

5.987 ± 0.042

6.773 ± 0.048

0.595 ± 0.015

4.199 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski