Spinybacked orbweaver circular virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses; Circularisvirus

Average proteome isoelectric point is 8.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPA1|A0A346BPA1_9VIRU Putative capsid protein OS=Spinybacked orbweaver circular virus 1 OX=2293305 PE=4 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84CHH5 pKa = 6.04GWTFTVFALEE15 pKa = 4.24PEE17 pKa = 4.59PVFEE21 pKa = 5.09PNLHH25 pKa = 6.64EE26 pKa = 3.83YY27 pKa = 10.77LIFGRR32 pKa = 11.84EE33 pKa = 3.97TCPTTGRR40 pKa = 11.84SHH42 pKa = 6.18LQGYY46 pKa = 9.92IYY48 pKa = 10.4FKK50 pKa = 10.21EE51 pKa = 4.07RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84LPYY57 pKa = 9.69LKK59 pKa = 10.06KK60 pKa = 9.48WIPGAHH66 pKa = 6.02FEE68 pKa = 4.14AAKK71 pKa = 10.69GNPEE75 pKa = 3.9QNQKK79 pKa = 10.31YY80 pKa = 9.46CSKK83 pKa = 11.11DD84 pKa = 3.12GDD86 pKa = 3.73FKK88 pKa = 11.41EE89 pKa = 4.29YY90 pKa = 10.93GRR92 pKa = 11.84LPRR95 pKa = 11.84SKK97 pKa = 10.59SGGGAFKK104 pKa = 10.81DD105 pKa = 3.5VLLKK109 pKa = 10.97AEE111 pKa = 4.72SGNIADD117 pKa = 4.8IKK119 pKa = 10.8EE120 pKa = 4.23EE121 pKa = 4.21YY122 pKa = 9.4PGLFIRR128 pKa = 11.84YY129 pKa = 6.7KK130 pKa = 9.9TNILSSVKK138 pKa = 10.11FRR140 pKa = 11.84VEE142 pKa = 3.98EE143 pKa = 4.14LKK145 pKa = 10.97SSCGVWICGPPRR157 pKa = 11.84CGKK160 pKa = 10.26DD161 pKa = 3.24YY162 pKa = 11.18AVRR165 pKa = 11.84KK166 pKa = 9.71LNSVYY171 pKa = 10.7VKK173 pKa = 10.27PLNKK177 pKa = 9.2WWDD180 pKa = 3.72GYY182 pKa = 10.78NNEE185 pKa = 4.18NFVLLSDD192 pKa = 4.36VEE194 pKa = 4.45PPHH197 pKa = 6.66CSWLGFFLKK206 pKa = 10.08IWTDD210 pKa = 3.27RR211 pKa = 11.84YY212 pKa = 11.26AFVAEE217 pKa = 4.17IKK219 pKa = 10.54GGSVKK224 pKa = 9.84IRR226 pKa = 11.84PKK228 pKa = 10.61KK229 pKa = 10.45IFVTSNFTLEE239 pKa = 3.93EE240 pKa = 4.25CFSGKK245 pKa = 9.15VLEE248 pKa = 4.26ALRR251 pKa = 11.84TRR253 pKa = 11.84FNVYY257 pKa = 10.75NEE259 pKa = 3.81FDD261 pKa = 3.27GSFFARR267 pKa = 11.84QVDD270 pKa = 4.28SIKK273 pKa = 10.0TAVYY277 pKa = 10.45DD278 pKa = 3.85KK279 pKa = 11.04LLSLEE284 pKa = 4.79DD285 pKa = 3.69GLVQEE290 pKa = 5.28KK291 pKa = 10.69EE292 pKa = 4.17NVSPSVSKK300 pKa = 11.23AEE302 pKa = 4.6EE303 pKa = 3.89ILSSSEE309 pKa = 3.62EE310 pKa = 3.97FEE312 pKa = 4.95NVQKK316 pKa = 10.57PSKK319 pKa = 9.87KK320 pKa = 9.82KK321 pKa = 10.26KK322 pKa = 9.94RR323 pKa = 11.84LSKK326 pKa = 9.92TNSAQQ331 pKa = 3.11

Molecular weight: 38.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPA1|A0A346BPA1_9VIRU Putative capsid protein OS=Spinybacked orbweaver circular virus 1 OX=2293305 PE=4 SV=1
MM1 pKa = 7.55AWFKK5 pKa = 10.36RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84MFRR11 pKa = 11.84RR12 pKa = 11.84LFRR15 pKa = 11.84KK16 pKa = 9.38RR17 pKa = 11.84KK18 pKa = 8.72KK19 pKa = 9.48FYY21 pKa = 9.89HH22 pKa = 6.03RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84NLRR28 pKa = 11.84MFRR31 pKa = 11.84NRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84NDD39 pKa = 2.84YY40 pKa = 10.53RR41 pKa = 11.84KK42 pKa = 8.42QTVLSDD48 pKa = 2.94ITYY51 pKa = 8.79TLDD54 pKa = 3.22VTHH57 pKa = 6.46EE58 pKa = 4.13QDD60 pKa = 4.76SKK62 pKa = 11.72SSTNNAEE69 pKa = 3.73MWGANCSLSALQTPEE84 pKa = 4.52FKK86 pKa = 10.07TLCKK90 pKa = 10.09DD91 pKa = 3.09WKK93 pKa = 9.01FCKK96 pKa = 9.97INSISYY102 pKa = 8.75ACTVGAIGYY111 pKa = 8.57NVYY114 pKa = 10.54EE115 pKa = 4.4PADD118 pKa = 3.56AKK120 pKa = 11.24AKK122 pKa = 10.05VQAQRR127 pKa = 11.84IDD129 pKa = 4.25LEE131 pKa = 4.42VNDD134 pKa = 4.25IVKK137 pKa = 9.78NQPFYY142 pKa = 10.67FIWDD146 pKa = 3.36ADD148 pKa = 3.6TRR150 pKa = 11.84LAEE153 pKa = 4.46SGNVNPNQLMSEE165 pKa = 4.31TSKK168 pKa = 10.58KK169 pKa = 9.95HH170 pKa = 6.42CIINGKK176 pKa = 9.82KK177 pKa = 9.84SAFFNLRR184 pKa = 11.84PPSILRR190 pKa = 11.84RR191 pKa = 11.84YY192 pKa = 8.24YY193 pKa = 10.52DD194 pKa = 3.18SKK196 pKa = 11.21VVYY199 pKa = 10.64NNRR202 pKa = 11.84NKK204 pKa = 8.97NTWGEE209 pKa = 4.14YY210 pKa = 10.01FDD212 pKa = 4.25NLSTISNYY220 pKa = 10.26RR221 pKa = 11.84MCNTFRR227 pKa = 11.84GTCGSYY233 pKa = 10.39FRR235 pKa = 11.84SLPIFVKK242 pKa = 10.62VDD244 pKa = 3.61DD245 pKa = 4.2TPVIYY250 pKa = 10.32KK251 pKa = 10.38CAMKK255 pKa = 10.72LILRR259 pKa = 11.84VKK261 pKa = 10.34CYY263 pKa = 10.8VNCTFKK269 pKa = 10.98GRR271 pKa = 11.84AYY273 pKa = 10.11EE274 pKa = 4.08YY275 pKa = 11.2NN276 pKa = 3.36

Molecular weight: 32.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

607

276

331

303.5

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.942 ± 0.332

2.965 ± 0.443

4.283 ± 0.532

6.26 ± 2.021

6.26 ± 0.312

5.272 ± 1.355

1.318 ± 0.156

4.613 ± 0.31

9.555 ± 0.579

7.084 ± 0.867

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.318 ± 0.821

6.59 ± 1.419

4.119 ± 0.822

2.306 ± 0.155

7.743 ± 1.862

7.578 ± 0.712

4.613 ± 0.798

6.096 ± 0.445

1.977 ± 0.111

5.107 ± 0.709
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski