Microbacterium phage Fede

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; unclassified Eekayvirinae

Average proteome isoelectric point is 5.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9UVK5|A0A7G9UVK5_9CAUD Cas4 family exonuclease OS=Microbacterium phage Fede OX=2767556 GN=12 PE=4 SV=1
MM1 pKa = 7.64SNNTDD6 pKa = 2.82PRR8 pKa = 11.84YY9 pKa = 9.94RR10 pKa = 11.84SGNFDD15 pKa = 3.22EE16 pKa = 5.24DD17 pKa = 3.24SRR19 pKa = 11.84EE20 pKa = 3.72IVLFFLGEE28 pKa = 4.06NEE30 pKa = 4.87KK31 pKa = 10.54PSAMYY36 pKa = 9.86VGCNGEE42 pKa = 3.7YY43 pKa = 10.6DD44 pKa = 3.47AFYY47 pKa = 11.19DD48 pKa = 4.01LDD50 pKa = 4.36ANIVGSPLSDD60 pKa = 3.45IIEE63 pKa = 4.44ATPEE67 pKa = 4.49DD68 pKa = 3.79IDD70 pKa = 3.97YY71 pKa = 11.13LKK73 pKa = 10.94VAAEE77 pKa = 3.97LQEE80 pKa = 4.28NGDD83 pKa = 3.8WNEE86 pKa = 3.88PTLVSLPSMFAVDD99 pKa = 3.89PWSGLSINMVQEE111 pKa = 4.73SAFTWLASQVYY122 pKa = 9.24SRR124 pKa = 11.84WYY126 pKa = 10.21DD127 pKa = 3.45DD128 pKa = 3.01TDD130 pKa = 3.27TRR132 pKa = 11.84TKK134 pKa = 9.94IARR137 pKa = 11.84EE138 pKa = 4.02LGEE141 pKa = 4.05HH142 pKa = 6.4AA143 pKa = 5.1

Molecular weight: 16.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9UVL0|A0A7G9UVL0_9CAUD Uncharacterized protein OS=Microbacterium phage Fede OX=2767556 GN=17 PE=4 SV=1
MM1 pKa = 7.0NTTPEE6 pKa = 3.82NDD8 pKa = 3.19RR9 pKa = 11.84TIVEE13 pKa = 4.46SIKK16 pKa = 9.93TRR18 pKa = 11.84IKK20 pKa = 10.48VVEE23 pKa = 4.29TPKK26 pKa = 10.83YY27 pKa = 7.45PTGTVIRR34 pKa = 11.84FNRR37 pKa = 11.84KK38 pKa = 9.14DD39 pKa = 3.34GNGVKK44 pKa = 10.0RR45 pKa = 11.84KK46 pKa = 9.42YY47 pKa = 10.26AALFAAKK54 pKa = 9.84RR55 pKa = 11.84WWTTSTLRR63 pKa = 11.84GIAIMGDD70 pKa = 3.36EE71 pKa = 4.55QFFDD75 pKa = 3.86MLGQPNVTKK84 pKa = 10.93VEE86 pKa = 4.08VATGFEE92 pKa = 4.38RR93 pKa = 11.84IKK95 pKa = 11.14

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

17212

40

4477

318.7

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.557 ± 0.387

0.471 ± 0.15

6.763 ± 0.251

6.17 ± 0.367

3.341 ± 0.127

7.733 ± 0.334

1.592 ± 0.163

4.973 ± 0.145

4.212 ± 0.383

8.07 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.141

4.66 ± 0.188

4.799 ± 0.226

4.764 ± 0.344

5.7 ± 0.396

6.193 ± 0.291

6.943 ± 0.367

6.693 ± 0.255

1.76 ± 0.126

3.108 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski