Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / CCUG 35730 / CIP 100753 / JCM 10117 / KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040) (Corynebacterium paurometabolum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Tsukamurellaceae; Tsukamurella; Tsukamurella paurometabola

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5UUH5|D5UUH5_TSUPD Uncharacterized protein OS=Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / CCUG 35730 / CIP 100753 / JCM 10117 / KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040) OX=521096 GN=Tpau_0912 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84NNLPPGLPPDD12 pKa = 4.75PFADD16 pKa = 4.31DD17 pKa = 4.46PSDD20 pKa = 3.41PTAALDD26 pKa = 4.47SLDD29 pKa = 4.33PGQPLDD35 pKa = 3.56PAEE38 pKa = 4.11RR39 pKa = 11.84LAVEE43 pKa = 4.64EE44 pKa = 4.68DD45 pKa = 3.51LADD48 pKa = 3.92LSVYY52 pKa = 9.82EE53 pKa = 4.49ALLGHH58 pKa = 6.61RR59 pKa = 11.84GIRR62 pKa = 11.84GLVICCDD69 pKa = 3.99DD70 pKa = 3.75CQEE73 pKa = 4.23DD74 pKa = 5.19HH75 pKa = 6.43YY76 pKa = 11.2HH77 pKa = 6.47DD78 pKa = 4.03WDD80 pKa = 3.61MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.33EE99 pKa = 4.4PAYY102 pKa = 10.75DD103 pKa = 4.0PAPDD107 pKa = 4.77SYY109 pKa = 10.62VTWDD113 pKa = 3.35YY114 pKa = 11.64CRR116 pKa = 11.84GYY118 pKa = 11.37ADD120 pKa = 4.29AAMNLAGEE128 pKa = 4.44EE129 pKa = 4.15

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5UPH2|D5UPH2_TSUPD Secreted protein OS=Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / CCUG 35730 / CIP 100753 / JCM 10117 / KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040) OX=521096 GN=Tpau_2117 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.88GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4170

0

4170

1342153

30

4264

321.9

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.916 ± 0.083

0.667 ± 0.009

6.22 ± 0.035

5.241 ± 0.036

2.975 ± 0.024

9.177 ± 0.035

1.956 ± 0.019

4.186 ± 0.027

2.189 ± 0.032

9.744 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.015

1.996 ± 0.019

5.776 ± 0.035

2.778 ± 0.024

7.388 ± 0.037

5.339 ± 0.021

6.219 ± 0.032

8.897 ± 0.039

1.41 ± 0.016

2.067 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski