Thermopolyspora flexuosa

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Thermopolyspora

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543J1D1|A0A543J1D1_9ACTN TetR family transcriptional regulator OS=Thermopolyspora flexuosa OX=103836 GN=FHX40_3372 PE=4 SV=1
MM1 pKa = 7.56SSIPEE6 pKa = 3.65EE7 pKa = 3.66LGYY10 pKa = 10.76SKK12 pKa = 10.36EE13 pKa = 4.3HH14 pKa = 6.15EE15 pKa = 4.44WVAGLDD21 pKa = 3.6DD22 pKa = 4.29GRR24 pKa = 11.84VVTIGITAYY33 pKa = 10.32AAEE36 pKa = 4.0QLGDD40 pKa = 3.62VVYY43 pKa = 10.98VQLPEE48 pKa = 4.02VGATVQAGDD57 pKa = 3.23AVGEE61 pKa = 4.3VEE63 pKa = 4.5SVKK66 pKa = 10.75SVSDD70 pKa = 3.67VYY72 pKa = 11.18CPVSGEE78 pKa = 3.97VVEE81 pKa = 4.5INEE84 pKa = 4.46AVVDD88 pKa = 4.47DD89 pKa = 4.64PSLINSDD96 pKa = 3.82PYY98 pKa = 11.36GEE100 pKa = 4.19GWLFKK105 pKa = 10.99VRR107 pKa = 11.84TTGEE111 pKa = 4.02AEE113 pKa = 3.97EE114 pKa = 4.34LLSAEE119 pKa = 4.52EE120 pKa = 4.14YY121 pKa = 10.76AALTAGDD128 pKa = 3.84SS129 pKa = 3.51

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543IPZ0|A0A543IPZ0_9ACTN Uncharacterized protein OS=Thermopolyspora flexuosa OX=103836 GN=FHX40_4769 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.93GRR40 pKa = 11.84KK41 pKa = 8.98RR42 pKa = 11.84LTVV45 pKa = 3.11

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4864

0

4864

1624665

29

2647

334.0

35.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.707 ± 0.056

0.816 ± 0.01

5.646 ± 0.025

6.098 ± 0.037

2.692 ± 0.017

9.302 ± 0.038

2.139 ± 0.017

3.769 ± 0.025

1.964 ± 0.026

10.32 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.731 ± 0.012

1.727 ± 0.017

6.501 ± 0.035

2.357 ± 0.019

9.182 ± 0.035

4.28 ± 0.023

5.554 ± 0.027

8.67 ± 0.036

1.449 ± 0.015

2.095 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski