Amino acid dipepetide frequency for Clostridium phage phi8074-B1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.069AlaAla: 0.069 ± 0.065
0.623AlaCys: 0.623 ± 0.224
2.632AlaAsp: 2.632 ± 0.413
4.363AlaGlu: 4.363 ± 0.544
1.801AlaPhe: 1.801 ± 0.443
3.186AlaGly: 3.186 ± 0.528
0.554AlaHis: 0.554 ± 0.218
4.294AlaIle: 4.294 ± 0.577
4.709AlaLys: 4.709 ± 0.748
4.086AlaLeu: 4.086 ± 0.592
1.454AlaMet: 1.454 ± 0.353
2.632AlaAsn: 2.632 ± 0.46
0.693AlaPro: 0.693 ± 0.224
1.87AlaGln: 1.87 ± 0.377
2.216AlaArg: 2.216 ± 0.472
2.701AlaSer: 2.701 ± 0.465
3.186AlaThr: 3.186 ± 0.529
2.77AlaVal: 2.77 ± 0.44
0.485AlaTrp: 0.485 ± 0.17
2.493AlaTyr: 2.493 ± 0.471
0.0AlaXaa: 0.0 ± 0.0
Cys
0.554CysAla: 0.554 ± 0.215
0.623CysCys: 0.623 ± 0.21
0.97CysAsp: 0.97 ± 0.279
0.831CysGlu: 0.831 ± 0.2
0.554CysPhe: 0.554 ± 0.18
0.97CysGly: 0.97 ± 0.328
0.416CysHis: 0.416 ± 0.192
0.9CysIle: 0.9 ± 0.231
1.039CysLys: 1.039 ± 0.291
0.97CysLeu: 0.97 ± 0.237
0.416CysMet: 0.416 ± 0.162
0.623CysAsn: 0.623 ± 0.19
0.693CysPro: 0.693 ± 0.182
0.346CysGln: 0.346 ± 0.154
0.485CysArg: 0.485 ± 0.206
0.623CysSer: 0.623 ± 0.214
0.693CysThr: 0.693 ± 0.222
0.831CysVal: 0.831 ± 0.223
0.208CysTrp: 0.208 ± 0.101
1.039CysTyr: 1.039 ± 0.268
0.0CysXaa: 0.0 ± 0.0
Asp
2.909AspAla: 2.909 ± 0.521
0.831AspCys: 0.831 ± 0.233
2.285AspAsp: 2.285 ± 0.397
4.086AspGlu: 4.086 ± 0.556
2.563AspPhe: 2.563 ± 0.527
2.424AspGly: 2.424 ± 0.362
0.693AspHis: 0.693 ± 0.186
6.441AspIle: 6.441 ± 0.784
6.995AspLys: 6.995 ± 0.601
6.095AspLeu: 6.095 ± 0.697
2.978AspMet: 2.978 ± 0.464
3.601AspAsn: 3.601 ± 0.567
1.87AspPro: 1.87 ± 0.259
1.524AspGln: 1.524 ± 0.268
3.532AspArg: 3.532 ± 0.422
2.355AspSer: 2.355 ± 0.327
4.709AspThr: 4.709 ± 0.519
3.74AspVal: 3.74 ± 0.345
0.693AspTrp: 0.693 ± 0.198
3.948AspTyr: 3.948 ± 0.52
0.0AspXaa: 0.0 ± 0.0
Glu
3.74GluAla: 3.74 ± 0.492
1.039GluCys: 1.039 ± 0.298
5.402GluAsp: 5.402 ± 0.704
6.856GluGlu: 6.856 ± 0.844
3.671GluPhe: 3.671 ± 0.527
4.571GluGly: 4.571 ± 0.68
1.939GluHis: 1.939 ± 0.412
6.856GluIle: 6.856 ± 0.578
6.025GluLys: 6.025 ± 0.74
10.112GluLeu: 10.112 ± 0.985
1.87GluMet: 1.87 ± 0.367
4.294GluAsn: 4.294 ± 0.452
1.731GluPro: 1.731 ± 0.442
3.463GluGln: 3.463 ± 0.525
2.493GluArg: 2.493 ± 0.457
3.948GluSer: 3.948 ± 0.56
4.64GluThr: 4.64 ± 0.469
5.887GluVal: 5.887 ± 0.664
0.831GluTrp: 0.831 ± 0.205
4.571GluTyr: 4.571 ± 0.482
0.0GluXaa: 0.0 ± 0.0
Phe
1.731PheAla: 1.731 ± 0.429
0.623PheCys: 0.623 ± 0.169
2.632PheAsp: 2.632 ± 0.374
3.324PheGlu: 3.324 ± 0.628
1.039PhePhe: 1.039 ± 0.265
2.909PheGly: 2.909 ± 0.614
0.762PheHis: 0.762 ± 0.256
1.939PheIle: 1.939 ± 0.422
3.601PheLys: 3.601 ± 0.479
2.632PheLeu: 2.632 ± 0.42
0.97PheMet: 0.97 ± 0.256
2.563PheAsn: 2.563 ± 0.479
1.593PhePro: 1.593 ± 0.335
0.97PheGln: 0.97 ± 0.254
1.593PheArg: 1.593 ± 0.299
1.731PheSer: 1.731 ± 0.328
2.216PheThr: 2.216 ± 0.522
1.593PheVal: 1.593 ± 0.302
0.554PheTrp: 0.554 ± 0.21
1.454PheTyr: 1.454 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
3.117GlyAla: 3.117 ± 0.464
0.554GlyCys: 0.554 ± 0.229
3.601GlyAsp: 3.601 ± 0.468
6.51GlyGlu: 6.51 ± 0.722
2.147GlyPhe: 2.147 ± 0.349
4.086GlyGly: 4.086 ± 0.579
1.316GlyHis: 1.316 ± 0.329
6.233GlyIle: 6.233 ± 0.637
5.125GlyLys: 5.125 ± 0.624
5.194GlyLeu: 5.194 ± 0.648
1.385GlyMet: 1.385 ± 0.251
3.601GlyAsn: 3.601 ± 0.743
0.0GlyPro: 0.0 ± 0.0
1.801GlyGln: 1.801 ± 0.405
2.84GlyArg: 2.84 ± 0.401
3.324GlySer: 3.324 ± 0.554
3.394GlyThr: 3.394 ± 0.474
3.255GlyVal: 3.255 ± 0.441
0.9GlyTrp: 0.9 ± 0.316
3.532GlyTyr: 3.532 ± 0.492
0.0GlyXaa: 0.0 ± 0.0
His
0.416HisAla: 0.416 ± 0.184
0.208HisCys: 0.208 ± 0.112
0.97HisAsp: 0.97 ± 0.227
1.108HisGlu: 1.108 ± 0.298
0.762HisPhe: 0.762 ± 0.195
1.108HisGly: 1.108 ± 0.252
0.416HisHis: 0.416 ± 0.167
2.008HisIle: 2.008 ± 0.386
1.454HisLys: 1.454 ± 0.272
2.147HisLeu: 2.147 ± 0.417
0.346HisMet: 0.346 ± 0.12
1.247HisAsn: 1.247 ± 0.251
0.346HisPro: 0.346 ± 0.167
0.485HisGln: 0.485 ± 0.173
0.485HisArg: 0.485 ± 0.159
0.762HisSer: 0.762 ± 0.26
1.108HisThr: 1.108 ± 0.248
1.177HisVal: 1.177 ± 0.321
0.208HisTrp: 0.208 ± 0.106
1.039HisTyr: 1.039 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
3.532IleAla: 3.532 ± 0.467
1.108IleCys: 1.108 ± 0.254
6.372IleAsp: 6.372 ± 0.924
6.579IleGlu: 6.579 ± 0.756
1.731IlePhe: 1.731 ± 0.331
4.017IleGly: 4.017 ± 0.624
1.662IleHis: 1.662 ± 0.424
4.502IleIle: 4.502 ± 0.602
6.164IleLys: 6.164 ± 0.539
7.133IleLeu: 7.133 ± 0.873
2.355IleMet: 2.355 ± 0.345
5.679IleAsn: 5.679 ± 0.696
3.324IlePro: 3.324 ± 0.56
2.701IleGln: 2.701 ± 0.522
3.601IleArg: 3.601 ± 0.491
4.571IleSer: 4.571 ± 0.622
5.264IleThr: 5.264 ± 0.733
4.709IleVal: 4.709 ± 0.606
0.416IleTrp: 0.416 ± 0.151
2.978IleTyr: 2.978 ± 0.454
0.0IleXaa: 0.0 ± 0.0
Lys
3.948LysAla: 3.948 ± 0.654
1.385LysCys: 1.385 ± 0.375
5.194LysAsp: 5.194 ± 0.606
6.302LysGlu: 6.302 ± 0.802
3.047LysPhe: 3.047 ± 0.406
4.363LysGly: 4.363 ± 0.479
1.731LysHis: 1.731 ± 0.379
5.333LysIle: 5.333 ± 0.551
4.779LysLys: 4.779 ± 0.568
9.211LysLeu: 9.211 ± 0.813
2.493LysMet: 2.493 ± 0.49
3.601LysAsn: 3.601 ± 0.443
3.186LysPro: 3.186 ± 0.509
5.056LysGln: 5.056 ± 0.739
2.77LysArg: 2.77 ± 0.407
3.74LysSer: 3.74 ± 0.607
5.056LysThr: 5.056 ± 0.504
5.194LysVal: 5.194 ± 0.651
0.762LysTrp: 0.762 ± 0.203
4.571LysTyr: 4.571 ± 0.61
0.0LysXaa: 0.0 ± 0.0
Leu
6.302LeuAla: 6.302 ± 1.108
1.385LeuCys: 1.385 ± 0.278
6.372LeuAsp: 6.372 ± 0.542
9.003LeuGlu: 9.003 ± 0.807
2.77LeuPhe: 2.77 ± 0.501
7.549LeuGly: 7.549 ± 0.685
1.177LeuHis: 1.177 ± 0.329
5.956LeuIle: 5.956 ± 0.591
6.718LeuLys: 6.718 ± 0.6
6.649LeuLeu: 6.649 ± 0.618
2.355LeuMet: 2.355 ± 0.385
5.264LeuAsn: 5.264 ± 0.452
2.493LeuPro: 2.493 ± 0.351
3.186LeuGln: 3.186 ± 0.475
3.878LeuArg: 3.878 ± 0.568
5.402LeuSer: 5.402 ± 0.588
4.432LeuThr: 4.432 ± 0.493
5.748LeuVal: 5.748 ± 0.734
0.9LeuTrp: 0.9 ± 0.308
4.086LeuTyr: 4.086 ± 0.572
0.0LeuXaa: 0.0 ± 0.0
Met
1.524MetAla: 1.524 ± 0.34
0.277MetCys: 0.277 ± 0.131
2.216MetAsp: 2.216 ± 0.344
1.593MetGlu: 1.593 ± 0.289
1.247MetPhe: 1.247 ± 0.344
2.285MetGly: 2.285 ± 0.42
0.139MetHis: 0.139 ± 0.098
1.524MetIle: 1.524 ± 0.454
3.047MetLys: 3.047 ± 0.408
2.008MetLeu: 2.008 ± 0.379
0.554MetMet: 0.554 ± 0.22
1.454MetAsn: 1.454 ± 0.304
0.554MetPro: 0.554 ± 0.206
0.693MetGln: 0.693 ± 0.29
1.177MetArg: 1.177 ± 0.302
1.593MetSer: 1.593 ± 0.44
1.662MetThr: 1.662 ± 0.273
1.524MetVal: 1.524 ± 0.359
0.277MetTrp: 0.277 ± 0.13
1.108MetTyr: 1.108 ± 0.31
0.0MetXaa: 0.0 ± 0.0
Asn
2.978AsnAla: 2.978 ± 0.466
0.693AsnCys: 0.693 ± 0.263
2.978AsnAsp: 2.978 ± 0.42
4.294AsnGlu: 4.294 ± 0.469
1.524AsnPhe: 1.524 ± 0.37
3.394AsnGly: 3.394 ± 0.498
1.177AsnHis: 1.177 ± 0.32
5.679AsnIle: 5.679 ± 0.63
4.917AsnLys: 4.917 ± 0.742
5.541AsnLeu: 5.541 ± 0.634
1.731AsnMet: 1.731 ± 0.343
2.84AsnAsn: 2.84 ± 0.399
2.493AsnPro: 2.493 ± 0.437
2.424AsnGln: 2.424 ± 0.473
2.84AsnArg: 2.84 ± 0.515
2.84AsnSer: 2.84 ± 0.616
2.84AsnThr: 2.84 ± 0.534
1.801AsnVal: 1.801 ± 0.259
0.554AsnTrp: 0.554 ± 0.212
3.601AsnTyr: 3.601 ± 0.506
0.0AsnXaa: 0.0 ± 0.0
Pro
0.97ProAla: 0.97 ± 0.208
0.208ProCys: 0.208 ± 0.136
1.524ProAsp: 1.524 ± 0.328
3.532ProGlu: 3.532 ± 0.546
1.108ProPhe: 1.108 ± 0.265
0.554ProGly: 0.554 ± 0.23
0.831ProHis: 0.831 ± 0.234
1.939ProIle: 1.939 ± 0.258
2.008ProLys: 2.008 ± 0.346
2.147ProLeu: 2.147 ± 0.417
1.039ProMet: 1.039 ± 0.268
1.801ProAsn: 1.801 ± 0.401
0.9ProPro: 0.9 ± 0.277
1.247ProGln: 1.247 ± 0.316
1.385ProArg: 1.385 ± 0.36
1.454ProSer: 1.454 ± 0.344
1.731ProThr: 1.731 ± 0.363
2.424ProVal: 2.424 ± 0.397
0.346ProTrp: 0.346 ± 0.166
1.524ProTyr: 1.524 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
2.355GlnAla: 2.355 ± 0.506
0.554GlnCys: 0.554 ± 0.194
2.701GlnAsp: 2.701 ± 0.421
4.017GlnGlu: 4.017 ± 0.494
1.316GlnPhe: 1.316 ± 0.285
2.355GlnGly: 2.355 ± 0.393
0.762GlnHis: 0.762 ± 0.204
3.809GlnIle: 3.809 ± 0.529
2.424GlnLys: 2.424 ± 0.398
4.225GlnLeu: 4.225 ± 0.547
0.97GlnMet: 0.97 ± 0.221
1.385GlnAsn: 1.385 ± 0.356
0.9GlnPro: 0.9 ± 0.239
1.939GlnGln: 1.939 ± 0.405
1.247GlnArg: 1.247 ± 0.232
1.524GlnSer: 1.524 ± 0.351
2.147GlnThr: 2.147 ± 0.421
1.801GlnVal: 1.801 ± 0.366
0.069GlnTrp: 0.069 ± 0.065
2.285GlnTyr: 2.285 ± 0.354
0.0GlnXaa: 0.0 ± 0.0
Arg
1.524ArgAla: 1.524 ± 0.367
0.485ArgCys: 0.485 ± 0.176
3.047ArgAsp: 3.047 ± 0.396
4.017ArgGlu: 4.017 ± 0.53
1.801ArgPhe: 1.801 ± 0.444
3.255ArgGly: 3.255 ± 0.458
0.346ArgHis: 0.346 ± 0.138
2.909ArgIle: 2.909 ± 0.392
3.809ArgLys: 3.809 ± 0.535
3.671ArgLeu: 3.671 ± 0.587
0.97ArgMet: 0.97 ± 0.226
2.147ArgAsn: 2.147 ± 0.373
1.316ArgPro: 1.316 ± 0.287
1.316ArgGln: 1.316 ± 0.348
2.078ArgArg: 2.078 ± 0.409
2.078ArgSer: 2.078 ± 0.377
2.84ArgThr: 2.84 ± 0.375
3.324ArgVal: 3.324 ± 0.697
0.554ArgTrp: 0.554 ± 0.187
2.285ArgTyr: 2.285 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
2.355SerAla: 2.355 ± 0.488
0.554SerCys: 0.554 ± 0.253
3.255SerAsp: 3.255 ± 0.485
3.809SerGlu: 3.809 ± 0.573
1.939SerPhe: 1.939 ± 0.271
3.394SerGly: 3.394 ± 0.549
0.623SerHis: 0.623 ± 0.213
3.74SerIle: 3.74 ± 0.493
3.74SerLys: 3.74 ± 0.559
4.432SerLeu: 4.432 ± 0.457
1.524SerMet: 1.524 ± 0.374
2.909SerAsn: 2.909 ± 0.436
1.524SerPro: 1.524 ± 0.302
2.147SerGln: 2.147 ± 0.382
2.147SerArg: 2.147 ± 0.398
2.701SerSer: 2.701 ± 0.448
2.701SerThr: 2.701 ± 0.492
3.601SerVal: 3.601 ± 0.534
0.623SerTrp: 0.623 ± 0.201
2.008SerTyr: 2.008 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
2.84ThrAla: 2.84 ± 0.391
1.108ThrCys: 1.108 ± 0.27
3.878ThrAsp: 3.878 ± 0.786
3.809ThrGlu: 3.809 ± 0.566
2.77ThrPhe: 2.77 ± 0.396
3.671ThrGly: 3.671 ± 0.491
1.454ThrHis: 1.454 ± 0.34
5.333ThrIle: 5.333 ± 0.578
4.571ThrLys: 4.571 ± 0.48
5.471ThrLeu: 5.471 ± 0.846
0.762ThrMet: 0.762 ± 0.216
3.532ThrAsn: 3.532 ± 0.49
2.355ThrPro: 2.355 ± 0.378
2.078ThrGln: 2.078 ± 0.355
2.632ThrArg: 2.632 ± 0.406
2.563ThrSer: 2.563 ± 0.435
3.671ThrThr: 3.671 ± 0.698
3.186ThrVal: 3.186 ± 0.397
0.554ThrTrp: 0.554 ± 0.216
2.493ThrTyr: 2.493 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
3.186ValAla: 3.186 ± 0.416
0.762ValCys: 0.762 ± 0.206
4.432ValAsp: 4.432 ± 0.555
5.056ValGlu: 5.056 ± 0.577
2.216ValPhe: 2.216 ± 0.406
3.671ValGly: 3.671 ± 0.481
0.762ValHis: 0.762 ± 0.248
4.502ValIle: 4.502 ± 0.788
5.125ValLys: 5.125 ± 0.516
3.948ValLeu: 3.948 ± 0.562
1.177ValMet: 1.177 ± 0.267
4.017ValAsn: 4.017 ± 0.557
1.731ValPro: 1.731 ± 0.269
3.324ValGln: 3.324 ± 0.557
3.255ValArg: 3.255 ± 0.571
3.117ValSer: 3.117 ± 0.538
3.047ValThr: 3.047 ± 0.453
3.532ValVal: 3.532 ± 0.604
0.554ValTrp: 0.554 ± 0.184
2.632ValTyr: 2.632 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
0.346TrpAla: 0.346 ± 0.149
0.139TrpCys: 0.139 ± 0.09
0.623TrpAsp: 0.623 ± 0.182
0.623TrpGlu: 0.623 ± 0.221
0.346TrpPhe: 0.346 ± 0.165
0.831TrpGly: 0.831 ± 0.23
0.208TrpHis: 0.208 ± 0.108
0.554TrpIle: 0.554 ± 0.173
1.247TrpLys: 1.247 ± 0.321
1.247TrpLeu: 1.247 ± 0.287
0.277TrpMet: 0.277 ± 0.141
0.693TrpAsn: 0.693 ± 0.182
0.0TrpPro: 0.0 ± 0.0
0.416TrpGln: 0.416 ± 0.193
0.139TrpArg: 0.139 ± 0.09
0.623TrpSer: 0.623 ± 0.188
0.485TrpThr: 0.485 ± 0.178
0.693TrpVal: 0.693 ± 0.211
0.277TrpTrp: 0.277 ± 0.118
0.277TrpTyr: 0.277 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.424TyrAla: 2.424 ± 0.406
0.623TyrCys: 0.623 ± 0.206
3.186TyrAsp: 3.186 ± 0.534
4.225TyrGlu: 4.225 ± 0.51
2.078TyrPhe: 2.078 ± 0.49
3.324TyrGly: 3.324 ± 0.544
0.831TyrHis: 0.831 ± 0.29
3.948TyrIle: 3.948 ± 0.478
4.294TyrLys: 4.294 ± 0.573
4.709TyrLeu: 4.709 ± 0.655
0.762TyrMet: 0.762 ± 0.284
3.255TyrAsn: 3.255 ± 0.386
0.97TyrPro: 0.97 ± 0.296
1.801TyrGln: 1.801 ± 0.377
2.978TyrArg: 2.978 ± 0.674
2.078TyrSer: 2.078 ± 0.348
2.909TyrThr: 2.909 ± 0.458
3.255TyrVal: 3.255 ± 0.386
0.277TyrTrp: 0.277 ± 0.201
2.008TyrTyr: 2.008 ± 0.469
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (14440 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski