candidate division TM6 bacterium JCVI TM6SC1

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Dependentiae; Vermiphilus; Vermiphilus pyriformis

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2I1X7|A0A0D2I1X7_9BACT Uncharacterized protein OS=candidate division TM6 bacterium JCVI TM6SC1 OX=1306947 GN=J120_02460 PE=4 SV=1
MM1 pKa = 7.48TSLEE5 pKa = 4.07QFNQFISDD13 pKa = 4.45FLNQMYY19 pKa = 10.32QPDD22 pKa = 3.63WHH24 pKa = 6.52MVSTDD29 pKa = 3.36QSLMEE34 pKa = 4.6ALCEE38 pKa = 4.04NLDD41 pKa = 3.61SVLYY45 pKa = 10.48HH46 pKa = 6.98LAFCSSIDD54 pKa = 3.36KK55 pKa = 10.25EE56 pKa = 4.53GRR58 pKa = 11.84VHH60 pKa = 7.3YY61 pKa = 9.24MGSLAFSYY69 pKa = 10.19QRR71 pKa = 11.84LMRR74 pKa = 11.84YY75 pKa = 7.09YY76 pKa = 8.41TFGMVTEE83 pKa = 4.87FVEE86 pKa = 4.47QAEE89 pKa = 4.42HH90 pKa = 6.85LEE92 pKa = 4.21KK93 pKa = 10.36TLGQLLRR100 pKa = 11.84HH101 pKa = 6.16PNFLVTAQAMLPDD114 pKa = 4.86LEE116 pKa = 4.51RR117 pKa = 11.84AMPQIEE123 pKa = 4.24QGTLGFGAMEE133 pKa = 4.41QISPALVLVSIGAVLADD150 pKa = 4.17GGNGSDD156 pKa = 4.85EE157 pKa = 4.98EE158 pKa = 5.08DD159 pKa = 3.62DD160 pKa = 4.2QEE162 pKa = 4.28

Molecular weight: 18.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2JN06|A0A0D2JN06_9BACT DsbD domain-containing protein OS=candidate division TM6 bacterium JCVI TM6SC1 OX=1306947 GN=J120_02130 PE=4 SV=1
MM1 pKa = 7.41SVTFFKK7 pKa = 10.8KK8 pKa = 9.9SRR10 pKa = 11.84KK11 pKa = 8.53KK12 pKa = 10.3RR13 pKa = 11.84NRR15 pKa = 11.84KK16 pKa = 9.13HH17 pKa = 6.31GFLKK21 pKa = 10.6RR22 pKa = 11.84MASHH26 pKa = 7.71DD27 pKa = 2.99GRR29 pKa = 11.84AIINRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ACKK39 pKa = 9.87RR40 pKa = 11.84KK41 pKa = 9.59RR42 pKa = 11.84LAVRR46 pKa = 11.84AA47 pKa = 4.0

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

860

0

860

269901

38

1440

313.8

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.313 ± 0.089

1.46 ± 0.036

4.67 ± 0.06

5.039 ± 0.065

4.264 ± 0.061

5.21 ± 0.095

2.627 ± 0.048

8.473 ± 0.082

6.11 ± 0.073

10.259 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.033

5.0 ± 0.088

3.853 ± 0.052

4.713 ± 0.064

4.069 ± 0.062

7.025 ± 0.08

6.255 ± 0.063

6.013 ± 0.076

1.122 ± 0.031

4.395 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski