Staphylococcus phage vB_SauS_fPfSau02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; Staphylococcus virus fPfSau02

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BM68|A0A411BM68_9CAUD dUTPase OS=Staphylococcus phage vB_SauS_fPfSau02 OX=2510149 GN=PFS2_057 PE=4 SV=1
MM1 pKa = 7.41ATQKK5 pKa = 10.18QVEE8 pKa = 4.59YY9 pKa = 11.35VMSLQEE15 pKa = 3.92QLEE18 pKa = 4.35LEE20 pKa = 4.45DD21 pKa = 3.95CEE23 pKa = 5.56KK24 pKa = 10.67YY25 pKa = 9.42TDD27 pKa = 3.83EE28 pKa = 4.01QVKK31 pKa = 10.4AMSHH35 pKa = 6.03KK36 pKa = 10.06EE37 pKa = 3.72VSNVIEE43 pKa = 4.41NYY45 pKa = 9.42KK46 pKa = 10.77ASISNEE52 pKa = 3.8EE53 pKa = 4.63LYY55 pKa = 10.85DD56 pKa = 3.48EE57 pKa = 4.93CMSFGLPNCC66 pKa = 4.51

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BM42|A0A411BM42_9CAUD Regulatory protein OS=Staphylococcus phage vB_SauS_fPfSau02 OX=2510149 GN=PFS2_030 PE=4 SV=1
MM1 pKa = 7.03LQKK4 pKa = 10.39FRR6 pKa = 11.84IAKK9 pKa = 8.54EE10 pKa = 3.78KK11 pKa = 11.12SKK13 pKa = 11.34LKK15 pKa = 11.0LNLLKK20 pKa = 10.54HH21 pKa = 6.14ANSNLEE27 pKa = 3.86TRR29 pKa = 11.84NNPEE33 pKa = 3.99LLRR36 pKa = 11.84AVAEE40 pKa = 4.04LLKK43 pKa = 10.65EE44 pKa = 4.17INRR47 pKa = 4.05

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13875

47

2066

204.0

23.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.557 ± 0.619

0.44 ± 0.098

6.321 ± 0.264

7.712 ± 0.398

3.762 ± 0.23

5.96 ± 0.545

1.636 ± 0.145

7.157 ± 0.374

10.169 ± 0.412

8.123 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.134

6.732 ± 0.24

2.573 ± 0.214

4.115 ± 0.209

3.935 ± 0.206

6.083 ± 0.355

5.816 ± 0.262

5.73 ± 0.227

1.196 ± 0.134

4.31 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski