Rubrivivax sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rubrivivax; unclassified Rubrivivax

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519ZPE6|A0A519ZPE6_9BURK Acyl-CoA desaturase OS=Rubrivivax sp. OX=50259 GN=EOP40_06510 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84IDD4 pKa = 3.76FNGLIDD10 pKa = 4.42LATAQRR16 pKa = 11.84TGARR20 pKa = 11.84LNALIDD26 pKa = 3.73FQPKK30 pKa = 9.23SGYY33 pKa = 10.77AITGYY38 pKa = 8.66TLTLDD43 pKa = 3.63MDD45 pKa = 3.7WPFIQGWSASQVLVSGSYY63 pKa = 10.87GFYY66 pKa = 10.87AEE68 pKa = 5.13DD69 pKa = 3.54WVPPGQLTLVFEE81 pKa = 4.55PRR83 pKa = 11.84TIFEE87 pKa = 4.77GPTAPRR93 pKa = 11.84IDD95 pKa = 3.56IDD97 pKa = 3.55ASTVSASVLEE107 pKa = 4.37CSVDD111 pKa = 3.87EE112 pKa = 5.76VWGEE116 pKa = 3.83WCGPTLEE123 pKa = 4.39VCSS126 pKa = 4.26

Molecular weight: 13.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519ZC85|A0A519ZC85_9BURK Amine oxidase (Fragment) OS=Rubrivivax sp. OX=50259 GN=EOP40_18275 PE=4 SV=1
FF1 pKa = 7.03VLRR4 pKa = 11.84EE5 pKa = 4.41FISLSPTRR13 pKa = 11.84RR14 pKa = 11.84GDD16 pKa = 3.24HH17 pKa = 6.63RR18 pKa = 11.84SLVLAFFIVLPLQYY32 pKa = 11.17ALVWTEE38 pKa = 3.68RR39 pKa = 11.84FNLFTVFIPVYY50 pKa = 9.75VFLLIPVVSALGNDD64 pKa = 3.54PQRR67 pKa = 11.84FLEE70 pKa = 4.7RR71 pKa = 11.84NAKK74 pKa = 8.31LQWGIMVCVYY84 pKa = 10.96GMSHH88 pKa = 6.37VPALLLLNFPGFAGKK103 pKa = 7.94TAFLVFFLVLVVQTCMLVQHH123 pKa = 6.08VAARR127 pKa = 11.84GLRR130 pKa = 11.84HH131 pKa = 6.08PAAPAISDD139 pKa = 3.8SFHH142 pKa = 5.11WRR144 pKa = 11.84SWAMGLAAGGLLGAVLSGFTPFKK167 pKa = 10.05PLPALAMALVACVAGSLGHH186 pKa = 7.05LVMKK190 pKa = 10.4AIKK193 pKa = 9.3RR194 pKa = 11.84DD195 pKa = 3.31RR196 pKa = 11.84GVTHH200 pKa = 7.2WGLGGRR206 pKa = 11.84SVTGASGLLDD216 pKa = 3.96RR217 pKa = 11.84VDD219 pKa = 4.38ALCFAAPVFFHH230 pKa = 5.68SVRR233 pKa = 11.84WTFNLL238 pKa = 3.61

Molecular weight: 26.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4239

0

4239

1279779

21

3697

301.9

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.929 ± 0.046

0.876 ± 0.013

5.269 ± 0.026

4.889 ± 0.031

3.291 ± 0.027

8.468 ± 0.038

2.463 ± 0.021

3.97 ± 0.027

2.71 ± 0.03

11.216 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.019

2.339 ± 0.024

5.565 ± 0.031

4.366 ± 0.024

6.972 ± 0.035

5.485 ± 0.026

5.257 ± 0.029

7.799 ± 0.027

1.655 ± 0.02

2.037 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski