Apis mellifera associated microvirus 48

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTP4|A0A3S8UTP4_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 48 OX=2494778 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 4.27FTMIKK7 pKa = 8.12DD8 pKa = 3.15TSMIYY13 pKa = 8.36QTLDD17 pKa = 3.28DD18 pKa = 4.99TPVSMPTRR26 pKa = 11.84LRR28 pKa = 11.84LPQSRR33 pKa = 11.84TEE35 pKa = 3.9QIRR38 pKa = 11.84QYY40 pKa = 10.81VRR42 pKa = 11.84EE43 pKa = 4.08EE44 pKa = 3.82MSRR47 pKa = 11.84AAAEE51 pKa = 3.95QGHH54 pKa = 5.99EE55 pKa = 4.33SFEE58 pKa = 4.21EE59 pKa = 4.73ADD61 pKa = 4.89DD62 pKa = 4.42FSLDD66 pKa = 4.23DD67 pKa = 4.56EE68 pKa = 4.97EE69 pKa = 6.33DD70 pKa = 3.83MPLSPYY76 pKa = 9.53EE77 pKa = 4.04LAMLEE82 pKa = 4.31PTPPLQNGVSAEE94 pKa = 4.07GAPPVGPAATSEE106 pKa = 4.4VPAASQPVTSEE117 pKa = 4.04TPPNVPP123 pKa = 3.94

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U4V2|A0A3Q8U4V2_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 48 OX=2494778 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.09CIKK5 pKa = 10.34LATRR9 pKa = 11.84TDD11 pKa = 3.75GFQFPCGQCKK21 pKa = 9.38NCRR24 pKa = 11.84INHH27 pKa = 5.14RR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 2.98WQSRR34 pKa = 11.84LLLEE38 pKa = 4.8ASSHH42 pKa = 4.9AHH44 pKa = 5.23SCFVTLTFADD54 pKa = 3.65TGIPPILRR62 pKa = 11.84RR63 pKa = 11.84SDD65 pKa = 3.13LKK67 pKa = 11.0RR68 pKa = 11.84FFRR71 pKa = 11.84ALRR74 pKa = 11.84VYY76 pKa = 10.51IPDD79 pKa = 3.42ARR81 pKa = 11.84HH82 pKa = 5.54FSVGEE87 pKa = 3.6YY88 pKa = 10.34GKK90 pKa = 10.64RR91 pKa = 11.84FGRR94 pKa = 11.84AHH96 pKa = 5.63YY97 pKa = 7.39HH98 pKa = 3.77THH100 pKa = 6.14IFSNVPVLATHH111 pKa = 7.59LAACWPFGHH120 pKa = 6.72VDD122 pKa = 3.26IGDD125 pKa = 3.93TEE127 pKa = 4.28PASLDD132 pKa = 3.4YY133 pKa = 10.99TLGYY137 pKa = 9.76LLKK140 pKa = 10.54DD141 pKa = 3.17KK142 pKa = 10.78RR143 pKa = 11.84SNVWPIEE150 pKa = 3.77SRR152 pKa = 11.84FPEE155 pKa = 4.13FRR157 pKa = 11.84AFSPGIGKK165 pKa = 8.41MALPHH170 pKa = 6.51LLIDD174 pKa = 4.22GNLLPRR180 pKa = 11.84EE181 pKa = 4.0YY182 pKa = 10.87KK183 pKa = 10.81VFGNLWPVSRR193 pKa = 11.84YY194 pKa = 9.23LRR196 pKa = 11.84QRR198 pKa = 11.84AKK200 pKa = 11.15KK201 pKa = 9.19MGFDD205 pKa = 3.58VSQTEE210 pKa = 4.2EE211 pKa = 3.77KK212 pKa = 10.52RR213 pKa = 11.84LEE215 pKa = 4.09EE216 pKa = 4.81FEE218 pKa = 4.21AQQMRR223 pKa = 11.84AVLRR227 pKa = 11.84NPSLTQAEE235 pKa = 4.65VSTLYY240 pKa = 11.01DD241 pKa = 3.24GFVKK245 pKa = 10.27KK246 pKa = 10.32RR247 pKa = 11.84QEE249 pKa = 3.82KK250 pKa = 11.14LEE252 pKa = 3.96VLKK255 pKa = 11.07KK256 pKa = 10.23KK257 pKa = 10.57AIRR260 pKa = 11.84DD261 pKa = 3.73AYY263 pKa = 9.74RR264 pKa = 11.84QIHH267 pKa = 5.14GHH269 pKa = 4.91VKK271 pKa = 8.87TRR273 pKa = 11.84SKK275 pKa = 11.53NEE277 pKa = 3.83TFF279 pKa = 3.71

Molecular weight: 32.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1344

123

495

268.8

29.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.896 ± 0.92

0.744 ± 0.397

4.911 ± 0.227

5.134 ± 1.082

4.464 ± 0.547

7.292 ± 0.997

2.307 ± 0.581

3.646 ± 0.216

4.241 ± 0.891

8.408 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.439

2.679 ± 0.494

6.771 ± 1.096

4.167 ± 0.407

8.185 ± 1.101

7.813 ± 0.892

6.92 ± 0.967

5.655 ± 0.397

1.042 ± 0.289

2.976 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski