Polyangium fumosum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Sorangiineae; Polyangiaceae; Polyangium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U1JGS0|A0A4U1JGS0_9DELT Uncharacterized protein OS=Polyangium fumosum OX=889272 GN=E8A74_06320 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.35RR3 pKa = 11.84SILVLGLLGTALAACGEE20 pKa = 4.6GAGLPPRR27 pKa = 11.84SNGSSSSTSSSAGPGGAGGQGGEE50 pKa = 4.48GGGGGIGGQGGAGGQGGAGGKK71 pKa = 6.4PTPVCGDD78 pKa = 3.75DD79 pKa = 4.38ALDD82 pKa = 4.19SGEE85 pKa = 4.32EE86 pKa = 4.03CDD88 pKa = 5.78DD89 pKa = 4.09GNTEE93 pKa = 4.6AGDD96 pKa = 4.02GCSPEE101 pKa = 4.26CTVEE105 pKa = 3.94LGEE108 pKa = 5.05IEE110 pKa = 4.98PNDD113 pKa = 4.12TPDD116 pKa = 3.0QASPYY121 pKa = 9.79KK122 pKa = 10.74DD123 pKa = 3.4PFPAKK128 pKa = 9.78IAPEE132 pKa = 4.16GDD134 pKa = 3.0VDD136 pKa = 4.59FVSFTVAATNTSVIARR152 pKa = 11.84ILDD155 pKa = 3.65VGDD158 pKa = 4.12GGCATGVIDD167 pKa = 5.19TILEE171 pKa = 3.83VRR173 pKa = 11.84GADD176 pKa = 3.25GVTVLASDD184 pKa = 4.21DD185 pKa = 3.89DD186 pKa = 4.74AGEE189 pKa = 4.55GACSRR194 pKa = 11.84AVLPSLAPGSYY205 pKa = 9.42FARR208 pKa = 11.84VVASGAAPSPTFLYY222 pKa = 10.3RR223 pKa = 11.84LRR225 pKa = 11.84IDD227 pKa = 3.56QVADD231 pKa = 3.35VCGDD235 pKa = 3.72GQKK238 pKa = 9.04TPAEE242 pKa = 4.28ACDD245 pKa = 5.12DD246 pKa = 4.18GNTDD250 pKa = 4.4AGDD253 pKa = 4.13GCSPTCSIEE262 pKa = 3.9ITEE265 pKa = 4.5TEE267 pKa = 4.42PNDD270 pKa = 3.79TPATANAFVAPWNGVLTPHH289 pKa = 7.09GDD291 pKa = 3.19VDD293 pKa = 4.26VVSVVLSGPASSLTATTTDD312 pKa = 2.96QGTNACAAKK321 pKa = 9.57TLDD324 pKa = 3.92TIVDD328 pKa = 3.67ILAPDD333 pKa = 4.12GTTVLATGDD342 pKa = 4.61DD343 pKa = 3.89IIGNCGSALAQNLTAGTYY361 pKa = 7.69FVRR364 pKa = 11.84MRR366 pKa = 11.84GGSLAGDD373 pKa = 4.06PSPYY377 pKa = 10.11GIQIVIQQ384 pKa = 3.63

Molecular weight: 37.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U1JJF1|A0A4U1JJF1_9DELT Uncharacterized protein OS=Polyangium fumosum OX=889272 GN=E8A74_03665 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 9.41MWTWRR7 pKa = 11.84PRR9 pKa = 11.84GPGRR13 pKa = 11.84RR14 pKa = 11.84PRR16 pKa = 11.84GPGRR20 pKa = 11.84RR21 pKa = 11.84PRR23 pKa = 11.84GPGRR27 pKa = 11.84RR28 pKa = 11.84PRR30 pKa = 11.84GPGRR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84GWRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84PRR44 pKa = 11.84GPGRR48 pKa = 11.84RR49 pKa = 11.84PRR51 pKa = 11.84GWRR54 pKa = 11.84GRR56 pKa = 11.84PRR58 pKa = 11.84GPGRR62 pKa = 11.84RR63 pKa = 11.84PRR65 pKa = 11.84GWRR68 pKa = 11.84GRR70 pKa = 11.84PRR72 pKa = 11.84GSGKK76 pKa = 10.44ASTRR80 pKa = 11.84LEE82 pKa = 3.97RR83 pKa = 11.84ASARR87 pKa = 11.84SGKK90 pKa = 10.48ASTRR94 pKa = 11.84FGKK97 pKa = 10.61ASTRR101 pKa = 11.84FGKK104 pKa = 10.56ASTRR108 pKa = 11.84FEE110 pKa = 3.98RR111 pKa = 11.84ASTRR115 pKa = 11.84FGKK118 pKa = 10.66APTRR122 pKa = 11.84SGKK125 pKa = 10.61ASTRR129 pKa = 3.5

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9827

0

9827

3775618

28

5848

384.2

41.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.897 ± 0.037

1.261 ± 0.019

5.68 ± 0.019

6.366 ± 0.027

3.302 ± 0.013

9.005 ± 0.032

2.049 ± 0.01

3.72 ± 0.015

3.145 ± 0.022

9.951 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.01

2.052 ± 0.016

6.398 ± 0.031

2.617 ± 0.012

7.941 ± 0.035

5.44 ± 0.015

5.263 ± 0.02

7.811 ± 0.02

1.255 ± 0.009

1.999 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski