Streptococcus phage phi-SsuFJSM8_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MKH4|A0A7G3MKH4_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJSM8_rum OX=2664395 PE=4 SV=1
MM1 pKa = 7.68ALLNIDD7 pKa = 3.96KK8 pKa = 9.38VTEE11 pKa = 4.25PFDD14 pKa = 4.72LEE16 pKa = 3.89TALAYY21 pKa = 8.8MRR23 pKa = 11.84KK24 pKa = 8.05NGEE27 pKa = 4.15FIRR30 pKa = 11.84CKK32 pKa = 9.37TAEE35 pKa = 3.98QDD37 pKa = 3.13FYY39 pKa = 11.24MYY41 pKa = 10.43LEE43 pKa = 4.31EE44 pKa = 4.27VRR46 pKa = 11.84RR47 pKa = 11.84PAIKK51 pKa = 9.97NGKK54 pKa = 7.82RR55 pKa = 11.84QLVTTEE61 pKa = 4.17TVWAFNQWGSTTLTLNLSDD80 pKa = 5.06LFHH83 pKa = 7.49DD84 pKa = 4.76CFYY87 pKa = 11.68LMRR90 pKa = 11.84FDD92 pKa = 5.99EE93 pKa = 4.69NGQPDD98 pKa = 3.69WSDD101 pKa = 3.12PSIVQEE107 pKa = 4.1GSVEE111 pKa = 4.34SEE113 pKa = 4.09VTDD116 pKa = 3.48EE117 pKa = 5.53GIVNTT122 pKa = 4.83

Molecular weight: 14.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MSB9|A0A7G3MSB9_9CAUD Portal protein OS=Streptococcus phage phi-SsuFJSM8_rum OX=2664395 PE=4 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84PSTPCKK10 pKa = 10.41QNGCPNLVPYY20 pKa = 8.56GHH22 pKa = 7.39KK23 pKa = 10.85YY24 pKa = 10.01CDD26 pKa = 3.27NHH28 pKa = 6.2KK29 pKa = 11.04ANYY32 pKa = 9.49QLDD35 pKa = 4.17TKK37 pKa = 8.57STKK40 pKa = 10.53AKK42 pKa = 10.29GYY44 pKa = 8.11NAQWNKK50 pKa = 9.57EE51 pKa = 3.62RR52 pKa = 11.84FRR54 pKa = 11.84YY55 pKa = 9.91LKK57 pKa = 9.8VHH59 pKa = 6.47PLCVQCKK66 pKa = 8.68VKK68 pKa = 10.55GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 4.74HH79 pKa = 6.91IKK81 pKa = 10.07PHH83 pKa = 6.85RR84 pKa = 11.84GDD86 pKa = 5.24QEE88 pKa = 5.02LFWNQSNWQALCKK101 pKa = 10.09SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.58RR113 pKa = 11.84YY114 pKa = 10.6VEE116 pKa = 3.73YY117 pKa = 9.41TYY119 pKa = 11.18RR120 pKa = 11.84FF121 pKa = 3.55

Molecular weight: 14.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

10233

43

1493

255.8

28.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.674 ± 0.575

0.84 ± 0.175

5.727 ± 0.273

7.515 ± 0.552

3.987 ± 0.296

6.196 ± 0.303

1.808 ± 0.139

7.134 ± 0.305

6.831 ± 0.284

9.127 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.212

4.876 ± 0.217

2.658 ± 0.199

4.613 ± 0.276

4.613 ± 0.341

7.104 ± 0.551

6.909 ± 0.609

6.225 ± 0.28

1.427 ± 0.099

3.557 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski