Sphingomonas sp. IBVSS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3925 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2Q6W6|A0A1Y2Q6W6_9SPHN 3-hydroxy-2-methylbutyryl-CoA dehydrogenase OS=Sphingomonas sp. IBVSS2 OX=1985172 GN=CAP40_18035 PE=4 SV=1
MM1 pKa = 8.11PDD3 pKa = 3.19PYY5 pKa = 10.98YY6 pKa = 11.06CKK8 pKa = 10.49LYY10 pKa = 10.96VDD12 pKa = 4.11TDD14 pKa = 3.81DD15 pKa = 6.94DD16 pKa = 4.03IGKK19 pKa = 10.01LEE21 pKa = 4.46AALDD25 pKa = 4.08DD26 pKa = 4.23AAGDD30 pKa = 3.63AFADD34 pKa = 3.39IRR36 pKa = 11.84VEE38 pKa = 4.01YY39 pKa = 9.64PVYY42 pKa = 10.43EE43 pKa = 4.28NEE45 pKa = 4.17NFDD48 pKa = 4.05PSSQAKK54 pKa = 9.48VPYY57 pKa = 10.21EE58 pKa = 4.01FIEE61 pKa = 4.25CSRR64 pKa = 11.84YY65 pKa = 9.17YY66 pKa = 10.96VEE68 pKa = 6.12LGTLEE73 pKa = 4.04QVPDD77 pKa = 3.62QLADD81 pKa = 3.93FQSGVATLVKK91 pKa = 10.12SLRR94 pKa = 11.84DD95 pKa = 3.18EE96 pKa = 4.44GRR98 pKa = 11.84FVTASCDD105 pKa = 3.8FEE107 pKa = 5.84DD108 pKa = 5.15VIADD112 pKa = 3.37ATGWNWTEE120 pKa = 4.57GNPEE124 pKa = 3.77PPGRR128 pKa = 11.84AVAAA132 pKa = 4.3

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2QJT1|A0A1Y2QJT1_9SPHN Short-chain dehydrogenase OS=Sphingomonas sp. IBVSS2 OX=1985172 GN=CAP40_08665 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3925

0

3925

1295300

29

6775

330.0

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.028 ± 0.055

0.68 ± 0.012

5.669 ± 0.029

5.159 ± 0.054

3.546 ± 0.023

9.446 ± 0.104

1.897 ± 0.022

4.769 ± 0.026

2.79 ± 0.031

9.952 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.024

2.601 ± 0.052

5.492 ± 0.048

3.048 ± 0.025

7.492 ± 0.059

4.964 ± 0.044

5.36 ± 0.071

7.091 ± 0.031

1.48 ± 0.022

2.258 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski