Amino acid dipepetide frequency for Gordonia phage Morrissey

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.367AlaAla: 15.367 ± 1.963
0.278AlaCys: 0.278 ± 0.154
7.093AlaAsp: 7.093 ± 0.665
7.788AlaGlu: 7.788 ± 0.837
3.06AlaPhe: 3.06 ± 0.581
10.43AlaGly: 10.43 ± 1.454
1.391AlaHis: 1.391 ± 0.349
3.964AlaIle: 3.964 ± 0.522
6.884AlaLys: 6.884 ± 0.771
8.692AlaLeu: 8.692 ± 0.664
2.434AlaMet: 2.434 ± 0.485
3.755AlaAsn: 3.755 ± 0.452
5.563AlaPro: 5.563 ± 0.803
4.242AlaGln: 4.242 ± 0.569
6.954AlaArg: 6.954 ± 0.717
6.606AlaSer: 6.606 ± 0.929
6.05AlaThr: 6.05 ± 0.92
8.831AlaVal: 8.831 ± 0.999
2.851AlaTrp: 2.851 ± 0.486
3.199AlaTyr: 3.199 ± 0.588
0.0AlaXaa: 0.0 ± 0.0
Cys
0.348CysAla: 0.348 ± 0.134
0.0CysCys: 0.0 ± 0.0
0.209CysAsp: 0.209 ± 0.111
0.556CysGlu: 0.556 ± 0.223
0.139CysPhe: 0.139 ± 0.099
0.695CysGly: 0.695 ± 0.224
0.139CysHis: 0.139 ± 0.096
0.139CysIle: 0.139 ± 0.109
0.278CysLys: 0.278 ± 0.187
0.348CysLeu: 0.348 ± 0.154
0.139CysMet: 0.139 ± 0.095
0.278CysAsn: 0.278 ± 0.131
0.487CysPro: 0.487 ± 0.232
0.209CysGln: 0.209 ± 0.12
0.626CysArg: 0.626 ± 0.288
0.626CysSer: 0.626 ± 0.205
0.209CysThr: 0.209 ± 0.132
0.139CysVal: 0.139 ± 0.094
0.209CysTrp: 0.209 ± 0.12
0.139CysTyr: 0.139 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
6.675AspAla: 6.675 ± 0.6
0.209AspCys: 0.209 ± 0.108
5.076AspAsp: 5.076 ± 1.557
3.268AspGlu: 3.268 ± 0.614
0.765AspPhe: 0.765 ± 0.22
5.563AspGly: 5.563 ± 0.594
1.669AspHis: 1.669 ± 0.363
2.434AspIle: 2.434 ± 0.4
2.017AspLys: 2.017 ± 0.461
6.536AspLeu: 6.536 ± 0.867
1.599AspMet: 1.599 ± 0.356
2.225AspAsn: 2.225 ± 0.564
4.728AspPro: 4.728 ± 0.466
1.738AspGln: 1.738 ± 0.381
4.52AspArg: 4.52 ± 0.658
2.99AspSer: 2.99 ± 0.514
4.52AspThr: 4.52 ± 0.687
3.338AspVal: 3.338 ± 0.403
1.53AspTrp: 1.53 ± 0.336
1.877AspTyr: 1.877 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
7.023GluAla: 7.023 ± 0.787
0.487GluCys: 0.487 ± 0.226
3.894GluAsp: 3.894 ± 0.619
4.45GluGlu: 4.45 ± 0.694
2.017GluPhe: 2.017 ± 0.419
4.659GluGly: 4.659 ± 0.548
1.599GluHis: 1.599 ± 0.411
3.546GluIle: 3.546 ± 0.599
0.765GluLys: 0.765 ± 0.254
5.146GluLeu: 5.146 ± 0.759
1.46GluMet: 1.46 ± 0.397
2.225GluAsn: 2.225 ± 0.431
3.964GluPro: 3.964 ± 0.565
1.808GluGln: 1.808 ± 0.338
4.033GluArg: 4.033 ± 0.542
3.964GluSer: 3.964 ± 0.582
3.268GluThr: 3.268 ± 0.549
3.894GluVal: 3.894 ± 0.534
1.321GluTrp: 1.321 ± 0.242
1.321GluTyr: 1.321 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
1.669PheAla: 1.669 ± 0.365
0.209PheCys: 0.209 ± 0.118
2.434PheAsp: 2.434 ± 0.388
1.252PheGlu: 1.252 ± 0.288
0.487PhePhe: 0.487 ± 0.181
1.599PheGly: 1.599 ± 0.312
0.556PheHis: 0.556 ± 0.273
1.391PheIle: 1.391 ± 0.332
1.46PheLys: 1.46 ± 0.342
2.017PheLeu: 2.017 ± 0.394
0.487PheMet: 0.487 ± 0.179
0.556PheAsn: 0.556 ± 0.188
2.364PhePro: 2.364 ± 0.349
1.391PheGln: 1.391 ± 0.299
1.877PheArg: 1.877 ± 0.306
1.53PheSer: 1.53 ± 0.307
1.669PheThr: 1.669 ± 0.357
1.321PheVal: 1.321 ± 0.366
0.348PheTrp: 0.348 ± 0.141
0.417PheTyr: 0.417 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
8.831GlyAla: 8.831 ± 1.14
0.626GlyCys: 0.626 ± 0.22
4.868GlyAsp: 4.868 ± 0.606
5.007GlyGlu: 5.007 ± 0.517
2.295GlyPhe: 2.295 ± 0.427
6.815GlyGly: 6.815 ± 1.047
1.808GlyHis: 1.808 ± 0.302
5.215GlyIle: 5.215 ± 0.778
3.755GlyLys: 3.755 ± 0.658
7.232GlyLeu: 7.232 ± 0.806
2.156GlyMet: 2.156 ± 0.441
2.851GlyAsn: 2.851 ± 0.514
3.964GlyPro: 3.964 ± 0.682
3.477GlyGln: 3.477 ± 0.49
6.328GlyArg: 6.328 ± 0.812
5.772GlySer: 5.772 ± 0.904
6.675GlyThr: 6.675 ± 0.968
6.745GlyVal: 6.745 ± 0.759
1.669GlyTrp: 1.669 ± 0.349
2.364GlyTyr: 2.364 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
1.599HisAla: 1.599 ± 0.298
0.139HisCys: 0.139 ± 0.089
1.321HisAsp: 1.321 ± 0.336
0.487HisGlu: 0.487 ± 0.2
0.626HisPhe: 0.626 ± 0.276
1.947HisGly: 1.947 ± 0.448
0.487HisHis: 0.487 ± 0.205
0.834HisIle: 0.834 ± 0.257
0.556HisLys: 0.556 ± 0.214
2.086HisLeu: 2.086 ± 0.45
0.487HisMet: 0.487 ± 0.185
0.974HisAsn: 0.974 ± 0.239
1.46HisPro: 1.46 ± 0.386
0.834HisGln: 0.834 ± 0.224
1.321HisArg: 1.321 ± 0.329
0.417HisSer: 0.417 ± 0.22
1.321HisThr: 1.321 ± 0.365
0.974HisVal: 0.974 ± 0.264
0.278HisTrp: 0.278 ± 0.127
0.07HisTyr: 0.07 ± 0.08
0.0HisXaa: 0.0 ± 0.0
Ile
3.616IleAla: 3.616 ± 0.809
0.0IleCys: 0.0 ± 0.0
2.573IleAsp: 2.573 ± 0.491
1.252IleGlu: 1.252 ± 0.237
0.695IlePhe: 0.695 ± 0.214
3.477IleGly: 3.477 ± 0.575
1.947IleHis: 1.947 ± 0.335
1.391IleIle: 1.391 ± 0.247
2.225IleLys: 2.225 ± 0.452
3.546IleLeu: 3.546 ± 0.477
0.556IleMet: 0.556 ± 0.188
0.626IleAsn: 0.626 ± 0.213
4.172IlePro: 4.172 ± 0.667
2.434IleGln: 2.434 ± 0.443
2.99IleArg: 2.99 ± 0.456
3.199IleSer: 3.199 ± 0.62
4.242IleThr: 4.242 ± 0.618
3.129IleVal: 3.129 ± 0.481
0.974IleTrp: 0.974 ± 0.227
1.043IleTyr: 1.043 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
7.023LysAla: 7.023 ± 0.991
0.278LysCys: 0.278 ± 0.136
3.06LysAsp: 3.06 ± 0.503
2.295LysGlu: 2.295 ± 0.405
1.043LysPhe: 1.043 ± 0.391
2.851LysGly: 2.851 ± 0.509
0.278LysHis: 0.278 ± 0.133
1.877LysIle: 1.877 ± 0.304
0.695LysLys: 0.695 ± 0.22
4.033LysLeu: 4.033 ± 0.49
1.043LysMet: 1.043 ± 0.286
1.252LysAsn: 1.252 ± 0.324
2.156LysPro: 2.156 ± 0.358
0.487LysGln: 0.487 ± 0.165
2.017LysArg: 2.017 ± 0.399
2.921LysSer: 2.921 ± 0.434
2.781LysThr: 2.781 ± 0.532
3.477LysVal: 3.477 ± 0.61
0.974LysTrp: 0.974 ± 0.331
0.765LysTyr: 0.765 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
9.387LeuAla: 9.387 ± 0.675
0.695LeuCys: 0.695 ± 0.222
5.285LeuAsp: 5.285 ± 0.535
6.05LeuGlu: 6.05 ± 0.771
1.947LeuPhe: 1.947 ± 0.338
7.371LeuGly: 7.371 ± 0.615
1.53LeuHis: 1.53 ± 0.298
2.99LeuIle: 2.99 ± 0.505
4.868LeuLys: 4.868 ± 0.597
5.424LeuLeu: 5.424 ± 0.712
1.53LeuMet: 1.53 ± 0.332
2.017LeuAsn: 2.017 ± 0.407
4.033LeuPro: 4.033 ± 0.638
2.086LeuGln: 2.086 ± 0.485
4.798LeuArg: 4.798 ± 0.658
3.824LeuSer: 3.824 ± 0.536
5.98LeuThr: 5.98 ± 0.573
5.563LeuVal: 5.563 ± 0.685
1.46LeuTrp: 1.46 ± 0.321
2.503LeuTyr: 2.503 ± 0.343
0.0LeuXaa: 0.0 ± 0.0
Met
3.407MetAla: 3.407 ± 0.664
0.139MetCys: 0.139 ± 0.106
1.252MetAsp: 1.252 ± 0.271
0.974MetGlu: 0.974 ± 0.273
0.626MetPhe: 0.626 ± 0.185
2.295MetGly: 2.295 ± 0.328
0.139MetHis: 0.139 ± 0.085
0.695MetIle: 0.695 ± 0.183
0.626MetLys: 0.626 ± 0.187
1.113MetLeu: 1.113 ± 0.309
0.487MetMet: 0.487 ± 0.206
0.695MetAsn: 0.695 ± 0.197
0.834MetPro: 0.834 ± 0.229
0.556MetGln: 0.556 ± 0.164
1.391MetArg: 1.391 ± 0.354
2.434MetSer: 2.434 ± 0.454
2.573MetThr: 2.573 ± 0.427
1.808MetVal: 1.808 ± 0.354
0.278MetTrp: 0.278 ± 0.136
0.278MetTyr: 0.278 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
3.338AsnAla: 3.338 ± 0.49
0.07AsnCys: 0.07 ± 0.071
1.877AsnAsp: 1.877 ± 0.411
0.765AsnGlu: 0.765 ± 0.216
0.626AsnPhe: 0.626 ± 0.19
3.964AsnGly: 3.964 ± 0.86
0.626AsnHis: 0.626 ± 0.185
1.738AsnIle: 1.738 ± 0.292
1.391AsnLys: 1.391 ± 0.277
2.503AsnLeu: 2.503 ± 0.375
0.417AsnMet: 0.417 ± 0.192
1.391AsnAsn: 1.391 ± 0.435
3.477AsnPro: 3.477 ± 0.438
0.834AsnGln: 0.834 ± 0.232
1.738AsnArg: 1.738 ± 0.406
2.017AsnSer: 2.017 ± 0.359
2.503AsnThr: 2.503 ± 0.448
2.503AsnVal: 2.503 ± 0.518
0.765AsnTrp: 0.765 ± 0.192
0.556AsnTyr: 0.556 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
6.397ProAla: 6.397 ± 0.729
0.417ProCys: 0.417 ± 0.248
5.493ProAsp: 5.493 ± 0.574
6.328ProGlu: 6.328 ± 1.035
1.808ProPhe: 1.808 ± 0.455
5.632ProGly: 5.632 ± 0.891
0.695ProHis: 0.695 ± 0.209
2.851ProIle: 2.851 ± 0.47
1.738ProLys: 1.738 ± 0.326
3.824ProLeu: 3.824 ± 0.539
1.182ProMet: 1.182 ± 0.253
2.086ProAsn: 2.086 ± 0.345
3.477ProPro: 3.477 ± 0.655
1.252ProGln: 1.252 ± 0.255
2.086ProArg: 2.086 ± 0.481
3.407ProSer: 3.407 ± 0.706
4.033ProThr: 4.033 ± 0.616
4.311ProVal: 4.311 ± 0.571
1.46ProTrp: 1.46 ± 0.383
1.252ProTyr: 1.252 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
4.868GlnAla: 4.868 ± 0.595
0.209GlnCys: 0.209 ± 0.13
1.46GlnAsp: 1.46 ± 0.309
1.53GlnGlu: 1.53 ± 0.283
1.46GlnPhe: 1.46 ± 0.299
2.851GlnGly: 2.851 ± 0.446
0.695GlnHis: 0.695 ± 0.218
2.573GlnIle: 2.573 ± 0.344
0.904GlnLys: 0.904 ± 0.202
2.851GlnLeu: 2.851 ± 0.503
0.834GlnMet: 0.834 ± 0.248
1.46GlnAsn: 1.46 ± 0.32
1.182GlnPro: 1.182 ± 0.39
0.834GlnGln: 0.834 ± 0.257
2.156GlnArg: 2.156 ± 0.364
1.53GlnSer: 1.53 ± 0.311
1.46GlnThr: 1.46 ± 0.314
2.921GlnVal: 2.921 ± 0.429
0.765GlnTrp: 0.765 ± 0.245
0.556GlnTyr: 0.556 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
6.536ArgAla: 6.536 ± 0.753
0.417ArgCys: 0.417 ± 0.204
3.546ArgAsp: 3.546 ± 0.576
4.172ArgGlu: 4.172 ± 0.692
1.46ArgPhe: 1.46 ± 0.381
6.397ArgGly: 6.397 ± 0.943
1.252ArgHis: 1.252 ± 0.326
1.46ArgIle: 1.46 ± 0.342
3.06ArgLys: 3.06 ± 0.438
4.52ArgLeu: 4.52 ± 0.544
2.295ArgMet: 2.295 ± 0.46
2.295ArgAsn: 2.295 ± 0.384
3.616ArgPro: 3.616 ± 0.564
2.225ArgGln: 2.225 ± 0.428
4.589ArgArg: 4.589 ± 0.907
3.129ArgSer: 3.129 ± 0.372
4.311ArgThr: 4.311 ± 0.594
5.354ArgVal: 5.354 ± 0.754
1.182ArgTrp: 1.182 ± 0.311
2.017ArgTyr: 2.017 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
8.414SerAla: 8.414 ± 0.721
0.348SerCys: 0.348 ± 0.183
3.964SerAsp: 3.964 ± 0.462
3.199SerGlu: 3.199 ± 0.516
1.53SerPhe: 1.53 ± 0.25
6.05SerGly: 6.05 ± 0.792
0.487SerHis: 0.487 ± 0.185
1.877SerIle: 1.877 ± 0.342
2.99SerLys: 2.99 ± 0.476
3.755SerLeu: 3.755 ± 0.5
1.043SerMet: 1.043 ± 0.315
2.017SerAsn: 2.017 ± 0.361
3.199SerPro: 3.199 ± 0.444
1.808SerGln: 1.808 ± 0.425
3.407SerArg: 3.407 ± 0.425
3.129SerSer: 3.129 ± 0.59
4.033SerThr: 4.033 ± 0.562
4.242SerVal: 4.242 ± 0.496
0.974SerTrp: 0.974 ± 0.25
0.834SerTyr: 0.834 ± 0.194
0.0SerXaa: 0.0 ± 0.0
Thr
8.901ThrAla: 8.901 ± 0.96
0.556ThrCys: 0.556 ± 0.211
3.268ThrAsp: 3.268 ± 0.359
4.242ThrGlu: 4.242 ± 0.593
1.599ThrPhe: 1.599 ± 0.318
7.719ThrGly: 7.719 ± 0.875
0.974ThrHis: 0.974 ± 0.261
2.851ThrIle: 2.851 ± 0.404
3.477ThrLys: 3.477 ± 0.529
5.354ThrLeu: 5.354 ± 0.824
1.321ThrMet: 1.321 ± 0.288
1.877ThrAsn: 1.877 ± 0.364
4.172ThrPro: 4.172 ± 0.538
2.156ThrGln: 2.156 ± 0.397
4.103ThrArg: 4.103 ± 0.569
3.129ThrSer: 3.129 ± 0.425
5.563ThrThr: 5.563 ± 0.691
6.467ThrVal: 6.467 ± 0.593
1.043ThrTrp: 1.043 ± 0.276
1.599ThrTyr: 1.599 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
8.066ValAla: 8.066 ± 0.891
0.556ValCys: 0.556 ± 0.271
4.589ValAsp: 4.589 ± 0.534
4.381ValGlu: 4.381 ± 0.56
1.321ValPhe: 1.321 ± 0.311
5.007ValGly: 5.007 ± 0.635
1.46ValHis: 1.46 ± 0.31
3.616ValIle: 3.616 ± 0.524
2.781ValLys: 2.781 ± 0.484
6.675ValLeu: 6.675 ± 0.697
2.364ValMet: 2.364 ± 0.397
2.573ValAsn: 2.573 ± 0.424
3.685ValPro: 3.685 ± 0.422
3.129ValGln: 3.129 ± 0.457
4.937ValArg: 4.937 ± 0.826
3.964ValSer: 3.964 ± 0.53
6.258ValThr: 6.258 ± 0.543
6.258ValVal: 6.258 ± 0.799
0.974ValTrp: 0.974 ± 0.288
1.947ValTyr: 1.947 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.391TrpAla: 1.391 ± 0.269
0.139TrpCys: 0.139 ± 0.099
0.765TrpAsp: 0.765 ± 0.24
1.391TrpGlu: 1.391 ± 0.266
0.626TrpPhe: 0.626 ± 0.359
1.46TrpGly: 1.46 ± 0.351
0.348TrpHis: 0.348 ± 0.141
1.391TrpIle: 1.391 ± 0.308
0.348TrpLys: 0.348 ± 0.151
1.53TrpLeu: 1.53 ± 0.295
0.487TrpMet: 0.487 ± 0.146
1.113TrpAsn: 1.113 ± 0.372
1.182TrpPro: 1.182 ± 0.32
0.765TrpGln: 0.765 ± 0.241
1.877TrpArg: 1.877 ± 0.346
1.391TrpSer: 1.391 ± 0.317
1.53TrpThr: 1.53 ± 0.313
1.599TrpVal: 1.599 ± 0.356
0.556TrpTrp: 0.556 ± 0.179
0.209TrpTyr: 0.209 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.573TyrAla: 2.573 ± 0.408
0.139TyrCys: 0.139 ± 0.089
1.252TyrAsp: 1.252 ± 0.347
1.46TyrGlu: 1.46 ± 0.375
0.904TyrPhe: 0.904 ± 0.258
1.669TyrGly: 1.669 ± 0.315
0.139TyrHis: 0.139 ± 0.12
1.252TyrIle: 1.252 ± 0.284
0.487TyrLys: 0.487 ± 0.212
2.156TyrLeu: 2.156 ± 0.384
0.209TyrMet: 0.209 ± 0.14
0.834TyrAsn: 0.834 ± 0.248
2.017TyrPro: 2.017 ± 0.383
0.834TyrGln: 0.834 ± 0.249
2.156TyrArg: 2.156 ± 0.401
1.321TyrSer: 1.321 ± 0.279
1.391TyrThr: 1.391 ± 0.297
1.669TyrVal: 1.669 ± 0.303
0.487TyrTrp: 0.487 ± 0.218
0.487TyrTyr: 0.487 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (14382 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski