Candidatus Accumulibacter sp. SK-12

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011NLL7|A0A011NLL7_9PROT UDP-glucose 6-dehydrogenase OS=Candidatus Accumulibacter sp. SK-12 OX=1454001 GN=tuaD PE=3 SV=1
MM1 pKa = 7.17NVATEE6 pKa = 4.03MPMPFVFTDD15 pKa = 3.72SAAGKK20 pKa = 9.3VKK22 pKa = 10.31EE23 pKa = 4.47LIEE26 pKa = 4.3EE27 pKa = 4.26EE28 pKa = 4.44GNPDD32 pKa = 2.85LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.9GFTFDD54 pKa = 4.44EE55 pKa = 4.63VANDD59 pKa = 5.14DD60 pKa = 4.04DD61 pKa = 4.76TALQKK66 pKa = 11.15NGVTLLVDD74 pKa = 3.69PMSYY78 pKa = 10.34QYY80 pKa = 11.57LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.39GSQFVIKK100 pKa = 10.57NPNASSTCGCGSSFSVV116 pKa = 3.54

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011N204|A0A011N204_9PROT Sensory transduction protein regX3 OS=Candidatus Accumulibacter sp. SK-12 OX=1454001 GN=regX3 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84LMTVSVIPGPSPARR16 pKa = 11.84PLPGLARR23 pKa = 11.84PAPGSGSHH31 pKa = 6.61APRR34 pKa = 11.84SNSHH38 pKa = 5.53GPRR41 pKa = 11.84FILHH45 pKa = 5.32VRR47 pKa = 11.84GFSLHH52 pKa = 5.54VPGFIAHH59 pKa = 5.72VPGSISHH66 pKa = 5.63VPGFISHH73 pKa = 6.78APGFISHH80 pKa = 7.22IPRR83 pKa = 11.84SISHH87 pKa = 6.12IPGFISHH94 pKa = 6.61TPGFISHH101 pKa = 6.52VPGCNVHH108 pKa = 6.27GAGSVAPVAAGRR120 pKa = 11.84RR121 pKa = 11.84VAARR125 pKa = 11.84YY126 pKa = 9.21RR127 pKa = 11.84ISAKK131 pKa = 9.82WFILRR136 pKa = 11.84RR137 pKa = 11.84NRR139 pKa = 11.84RR140 pKa = 11.84ARR142 pKa = 11.84RR143 pKa = 11.84STGGRR148 pKa = 11.84PSSWLRR154 pKa = 11.84SAGRR158 pKa = 11.84RR159 pKa = 11.84CVSPRR164 pKa = 11.84PSLPCARR171 pKa = 11.84QSARR175 pKa = 11.84APSPPAADD183 pKa = 3.33RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84ARR188 pKa = 11.84LAAVAA193 pKa = 4.44

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3906

0

3906

1261008

29

6490

322.8

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.939 ± 0.052

1.11 ± 0.017

5.45 ± 0.034

5.546 ± 0.041

3.465 ± 0.025

8.316 ± 0.046

2.278 ± 0.019

4.425 ± 0.028

2.433 ± 0.034

11.519 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.022

2.346 ± 0.027

5.218 ± 0.033

3.774 ± 0.024

8.289 ± 0.049

5.509 ± 0.031

4.494 ± 0.039

7.334 ± 0.035

1.396 ± 0.018

2.094 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski