Streptomyces sp. SCSIO 3032

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W7CZK4|A0A1W7CZK4_9ACTN SAM-dependent methyltransferase OS=Streptomyces sp. SCSIO 3032 OX=1264596 GN=CAG99_16560 PE=4 SV=1
MM1 pKa = 7.24SQPPPPPQNPPGPPQPEE18 pKa = 4.29PAAGPAPAPGGPAPASGGPAPASGGPASGTPAPAPGGPAPASGGPASGTPAPAPGGPAPASGGPASGTPAPAPSGPAPASGGPASGTPAPAPGTPASAPPAVPGGLPGQPGYY130 pKa = 11.02GYY132 pKa = 8.74PQQAYY137 pKa = 9.77GSPQQGYY144 pKa = 8.57GFPQSPGAAPYY155 pKa = 8.65PSPYY159 pKa = 9.04QQPGQPGPTAPGGAFGAPTAPGPYY183 pKa = 9.99GPATGGFATPPPGPGGPKK201 pKa = 9.2GRR203 pKa = 11.84GRR205 pKa = 11.84RR206 pKa = 11.84GVVLAAVAAFAVLVGGGGAVWLLTGVQDD234 pKa = 5.25DD235 pKa = 4.3PAPAAGDD242 pKa = 3.93GADD245 pKa = 3.87GGADD249 pKa = 3.53DD250 pKa = 4.72GAEE253 pKa = 3.99GGARR257 pKa = 11.84GEE259 pKa = 4.33AGNGAGLPLPTEE271 pKa = 4.88AIGASLAWEE280 pKa = 4.53VPAPEE285 pKa = 4.0VSEE288 pKa = 4.55DD289 pKa = 3.74EE290 pKa = 4.6NIIDD294 pKa = 5.95AKK296 pKa = 9.47GTWLIEE302 pKa = 4.03DD303 pKa = 3.48AFVRR307 pKa = 11.84VTPDD311 pKa = 2.87SLVSYY316 pKa = 10.69DD317 pKa = 4.05LATGQEE323 pKa = 4.13NWSLPFEE330 pKa = 4.35FSGGDD335 pKa = 3.67CLPSSNVSDD344 pKa = 3.45NRR346 pKa = 11.84VALLQGRR353 pKa = 11.84DD354 pKa = 3.84CEE356 pKa = 4.43VLTVVDD362 pKa = 4.25IAAGTEE368 pKa = 4.19VMSMQIEE375 pKa = 4.68SEE377 pKa = 4.22WPTSAGSVPAILGDD391 pKa = 3.78TVAVGTGVAGLGFSISRR408 pKa = 11.84QEE410 pKa = 4.31MIWRR414 pKa = 11.84PPANSDD420 pKa = 3.87CRR422 pKa = 11.84EE423 pKa = 3.75SDD425 pKa = 3.86YY426 pKa = 11.61LVVDD430 pKa = 4.34DD431 pKa = 5.2MFVSQLSCGFAGDD444 pKa = 3.53EE445 pKa = 4.47GGSIRR450 pKa = 11.84ATDD453 pKa = 3.48EE454 pKa = 4.46SGDD457 pKa = 3.72TEE459 pKa = 4.19LWEE462 pKa = 3.96WAYY465 pKa = 10.69GPTYY469 pKa = 9.96EE470 pKa = 4.81GEE472 pKa = 4.11PFEE475 pKa = 4.37VQSVVSVDD483 pKa = 3.66PLVVTAAVGEE493 pKa = 4.76DD494 pKa = 3.72YY495 pKa = 10.19TAADD499 pKa = 3.75HH500 pKa = 7.19ARR502 pKa = 11.84VLVIDD507 pKa = 4.2EE508 pKa = 4.28DD509 pKa = 4.19HH510 pKa = 6.91EE511 pKa = 4.47EE512 pKa = 4.19VAVEE516 pKa = 4.7LDD518 pKa = 3.55YY519 pKa = 11.66DD520 pKa = 3.49QDD522 pKa = 3.94RR523 pKa = 11.84YY524 pKa = 10.18MRR526 pKa = 11.84PCEE529 pKa = 3.94TNSFDD534 pKa = 3.94NCWLGVEE541 pKa = 4.64HH542 pKa = 7.3EE543 pKa = 4.69GFLYY547 pKa = 10.73LPSNVPYY554 pKa = 10.73GDD556 pKa = 3.7NAVEE560 pKa = 4.86AFDD563 pKa = 5.59LSTGQPLWEE572 pKa = 4.11VPAINGGQILPFGVQDD588 pKa = 4.07GQILAYY594 pKa = 9.88QVASTEE600 pKa = 4.1LEE602 pKa = 4.17GMVVAIDD609 pKa = 3.64PATEE613 pKa = 3.84EE614 pKa = 4.05VRR616 pKa = 11.84PIMALDD622 pKa = 3.75RR623 pKa = 11.84AARR626 pKa = 11.84PQEE629 pKa = 3.94HH630 pKa = 6.82ALMSGLFVHH639 pKa = 6.94DD640 pKa = 3.14QHH642 pKa = 8.66IIWRR646 pKa = 11.84DD647 pKa = 3.31NTLVLANRR655 pKa = 11.84SFYY658 pKa = 11.21SSDD661 pKa = 3.58GEE663 pKa = 4.23DD664 pKa = 3.25VRR666 pKa = 11.84SVLVYY671 pKa = 10.38RR672 pKa = 4.98

Molecular weight: 67.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W7CYE1|A0A1W7CYE1_9ACTN Uncharacterized protein OS=Streptomyces sp. SCSIO 3032 OX=1264596 GN=CAG99_13450 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.82GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5248

0

5248

1788051

29

7193

340.7

36.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.667 ± 0.06

0.75 ± 0.009

6.052 ± 0.029

5.953 ± 0.032

2.658 ± 0.018

9.97 ± 0.042

2.373 ± 0.017

2.828 ± 0.022

1.335 ± 0.019

10.408 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.613 ± 0.015

1.58 ± 0.019

6.463 ± 0.037

2.435 ± 0.02

8.897 ± 0.045

4.515 ± 0.024

5.777 ± 0.031

8.341 ± 0.033

1.521 ± 0.014

1.862 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski