Gordonia bronchialis (strain ATCC 25592 / DSM 43247 / BCRC 13721 / JCM 3198 / KCTC 3076 / NBRC 16047 / NCTC 10667) (Rhodococcus bronchialis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia bronchialis

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0LAA9|D0LAA9_GORB4 Uncharacterized protein OS=Gordonia bronchialis (strain ATCC 25592 / DSM 43247 / BCRC 13721 / JCM 3198 / KCTC 3076 / NBRC 16047 / NCTC 10667) OX=526226 GN=Gbro_0086 PE=4 SV=1
MM1 pKa = 7.45PWIIAGVTAVVLIAVVAVVAVVLRR25 pKa = 11.84SGSDD29 pKa = 3.11STTTAGASTTEE40 pKa = 4.19TNATEE45 pKa = 4.44SSATDD50 pKa = 3.34TTQEE54 pKa = 4.03TTAEE58 pKa = 4.31TTTEE62 pKa = 4.15PVTQLTGTWRR72 pKa = 11.84GPAAGDD78 pKa = 3.2QTGFDD83 pKa = 3.75VVATISSEE91 pKa = 4.25YY92 pKa = 9.65PLAATVEE99 pKa = 4.3YY100 pKa = 9.44PQIGCAGDD108 pKa = 3.13WSEE111 pKa = 6.35DD112 pKa = 3.29SRR114 pKa = 11.84QGQTLLLTEE123 pKa = 5.29TITSGTCVTSQITLTPVSDD142 pKa = 4.95DD143 pKa = 3.46EE144 pKa = 4.61LSFYY148 pKa = 10.98SSYY151 pKa = 11.27YY152 pKa = 9.75SQSQSRR158 pKa = 11.84TLVITSTLRR167 pKa = 11.84RR168 pKa = 11.84QQ169 pKa = 3.23

Molecular weight: 17.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0L9H5|D0L9H5_GORB4 Peptide chain release factor 1 OS=Gordonia bronchialis (strain ATCC 25592 / DSM 43247 / BCRC 13721 / JCM 3198 / KCTC 3076 / NBRC 16047 / NCTC 10667) OX=526226 GN=prfA PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNSRR37 pKa = 11.84RR38 pKa = 11.84TKK40 pKa = 10.54GRR42 pKa = 11.84AKK44 pKa = 9.64LTAA47 pKa = 4.21

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4628

0

4628

1506137

30

11233

325.4

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.85 ± 0.044

0.761 ± 0.011

6.79 ± 0.029

5.09 ± 0.03

2.931 ± 0.021

8.892 ± 0.038

2.231 ± 0.018

4.561 ± 0.025

2.046 ± 0.029

9.427 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.017

2.093 ± 0.019

5.698 ± 0.031

2.745 ± 0.019

7.536 ± 0.038

5.608 ± 0.023

6.446 ± 0.027

8.804 ± 0.045

1.459 ± 0.017

2.046 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski