Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509) (Actinomadura dassonvillei)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; Nocardiopsis dassonvillei; Nocardiopsis dassonvillei subsp. dassonvillei

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7AYD5|D7AYD5_NOCDD Transcriptional regulator TetR family OS=Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509) OX=446468 GN=Ndas_0675 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84VRR4 pKa = 11.84RR5 pKa = 11.84ISTILGGTAALAFALSACGTADD27 pKa = 3.09VGGGSGGEE35 pKa = 4.12GGGGEE40 pKa = 4.39GGGDD44 pKa = 3.72TITIGVKK51 pKa = 9.95YY52 pKa = 9.32DD53 pKa = 3.47QPGLGLLDD61 pKa = 3.69GDD63 pKa = 4.28APVGFDD69 pKa = 3.63VDD71 pKa = 3.77VATYY75 pKa = 9.9IAGEE79 pKa = 4.01LGYY82 pKa = 10.55EE83 pKa = 4.15PEE85 pKa = 4.14QIEE88 pKa = 4.21WTEE91 pKa = 3.56AASANRR97 pKa = 11.84EE98 pKa = 4.07SFLQQGTVDD107 pKa = 4.09MVIATYY113 pKa = 10.53SITDD117 pKa = 3.23EE118 pKa = 3.92RR119 pKa = 11.84RR120 pKa = 11.84QVVDD124 pKa = 3.69FAGPYY129 pKa = 9.6YY130 pKa = 10.51VAQQDD135 pKa = 3.78ILVQADD141 pKa = 3.49NEE143 pKa = 4.74DD144 pKa = 3.42IQGPEE149 pKa = 3.85DD150 pKa = 3.42LAGKK154 pKa = 7.48VLCSASGSRR163 pKa = 11.84SANNIVDD170 pKa = 3.96DD171 pKa = 4.0MGIDD175 pKa = 3.43AEE177 pKa = 4.2LRR179 pKa = 11.84EE180 pKa = 4.33AGNYY184 pKa = 8.72SEE186 pKa = 5.36CLSLLGNGTVDD197 pKa = 5.55AVTTDD202 pKa = 3.32NTILAGFAAQDD213 pKa = 3.46PGTYY217 pKa = 8.65RR218 pKa = 11.84VVNNPFGEE226 pKa = 3.99EE227 pKa = 4.2RR228 pKa = 11.84YY229 pKa = 10.31GVGLPAGSTEE239 pKa = 3.79RR240 pKa = 11.84CEE242 pKa = 4.25EE243 pKa = 3.92VNAAIQKK250 pKa = 8.31MWDD253 pKa = 2.84EE254 pKa = 4.94GYY256 pKa = 10.88AVEE259 pKa = 4.79ALEE262 pKa = 4.29TAFGEE267 pKa = 4.04TDD269 pKa = 3.54FAYY272 pKa = 10.2EE273 pKa = 3.85EE274 pKa = 4.19TAPEE278 pKa = 3.79VDD280 pKa = 3.19ACQQ283 pKa = 3.25

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7AX38|D7AX38_NOCDD Non-specific serine/threonine protein kinase OS=Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509) OX=446468 GN=Ndas_4419 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 7.46RR23 pKa = 11.84TRR25 pKa = 11.84VARR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.94KK32 pKa = 9.77

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5497

0

5497

1834850

30

7422

333.8

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.107 ± 0.051

0.705 ± 0.01

6.158 ± 0.03

6.542 ± 0.032

2.747 ± 0.018

9.798 ± 0.034

2.277 ± 0.017

2.804 ± 0.026

1.396 ± 0.02

10.275 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.015

1.721 ± 0.017

6.191 ± 0.033

2.445 ± 0.02

8.538 ± 0.037

5.256 ± 0.026

5.752 ± 0.024

8.988 ± 0.038

1.494 ± 0.014

1.962 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski