Pseudomonas phage vB_PaeP_PAO1_1-15pyo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus; Pseudomonas virus 15pyo

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IUX8|A0A0A1IUX8_9CAUD Putative DNA maturase B OS=Pseudomonas phage vB_PaeP_PAO1_1-15pyo OX=1548908 GN=ORF44 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84RR3 pKa = 11.84EE4 pKa = 4.01DD5 pKa = 3.65VFVQVDD11 pKa = 4.16KK12 pKa = 11.22ILTTDD17 pKa = 3.65YY18 pKa = 10.74TWVDD22 pKa = 3.16NTNIQLAVVPKK33 pKa = 9.93KK34 pKa = 10.33DD35 pKa = 3.18QEE37 pKa = 4.01VRR39 pKa = 11.84IFRR42 pKa = 11.84DD43 pKa = 3.52TPAQVPDD50 pKa = 3.7TQFSQGIPFLPRR62 pKa = 11.84YY63 pKa = 9.44IDD65 pKa = 3.78ANNKK69 pKa = 7.73QLLYY73 pKa = 10.69AVQEE77 pKa = 4.83GINTANLALDD87 pKa = 4.02GVLDD91 pKa = 5.11AIRR94 pKa = 11.84IAEE97 pKa = 4.12EE98 pKa = 3.63ARR100 pKa = 11.84RR101 pKa = 11.84LAQEE105 pKa = 4.42ALDD108 pKa = 4.19AANEE112 pKa = 4.02ALRR115 pKa = 11.84RR116 pKa = 11.84ALGFAEE122 pKa = 3.91IRR124 pKa = 11.84TITEE128 pKa = 3.68DD129 pKa = 3.21TDD131 pKa = 3.5ISKK134 pKa = 10.01EE135 pKa = 3.61WRR137 pKa = 11.84GYY139 pKa = 7.29WNRR142 pKa = 11.84CMTADD147 pKa = 3.68KK148 pKa = 10.28PITVTMQMEE157 pKa = 4.65DD158 pKa = 4.14PDD160 pKa = 4.64DD161 pKa = 3.75PWIEE165 pKa = 3.77FSEE168 pKa = 4.39VHH170 pKa = 6.26FEE172 pKa = 3.97QAGVRR177 pKa = 11.84DD178 pKa = 4.25LNIVAGPGVTINRR191 pKa = 11.84LQNTTMQLYY200 pKa = 10.33GEE202 pKa = 4.7NGVCTLKK209 pKa = 10.97RR210 pKa = 11.84LGPNHH215 pKa = 6.05WVVFGAMEE223 pKa = 4.71DD224 pKa = 3.79EE225 pKa = 4.44

Molecular weight: 25.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IX59|A0A0A1IX59_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_PAO1_1-15pyo OX=1548908 GN=ORF08 PE=4 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84VNEE6 pKa = 3.83LTSRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 6.94AAKK16 pKa = 9.72ARR18 pKa = 11.84RR19 pKa = 11.84DD20 pKa = 3.14KK21 pKa = 11.19ARR23 pKa = 11.84RR24 pKa = 11.84IDD26 pKa = 4.0LAHH29 pKa = 7.1KK30 pKa = 8.63MPKK33 pKa = 10.43GADD36 pKa = 3.22CPIFRR41 pKa = 11.84KK42 pKa = 10.22AEE44 pKa = 3.73QAQAKK49 pKa = 7.98QPRR52 pKa = 11.84VDD54 pKa = 3.31TLTTPRR60 pKa = 11.84SAGYY64 pKa = 9.29LAAAAYY70 pKa = 9.74LNKK73 pKa = 10.47SII75 pKa = 5.11

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13186

64

1255

269.1

29.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.307 ± 0.447

1.016 ± 0.179

5.771 ± 0.207

5.991 ± 0.246

3.041 ± 0.153

8.16 ± 0.319

2.199 ± 0.173

4.065 ± 0.166

4.55 ± 0.269

8.797 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.026 ± 0.114

3.435 ± 0.217

4.861 ± 0.351

4.763 ± 0.257

7.349 ± 0.349

4.823 ± 0.187

5.597 ± 0.273

6.666 ± 0.222

1.54 ± 0.118

3.003 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski