Microbulbifer agarilyticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Microbulbiferaceae; Microbulbifer

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2M9N2|A0A1Q2M9N2_9GAMM VWFA domain-containing protein OS=Microbulbifer agarilyticus OX=260552 GN=Mag101_05340 PE=4 SV=1
MM1 pKa = 6.78EE2 pKa = 4.28TSFAIIEE9 pKa = 4.01VDD11 pKa = 3.61EE12 pKa = 4.51AATAYY17 pKa = 8.29EE18 pKa = 3.91QLVTINDD25 pKa = 3.73YY26 pKa = 11.77AEE28 pKa = 4.49VPVAWSKK35 pKa = 10.63WSGDD39 pKa = 3.53PATTAQYY46 pKa = 11.19LLNGQVVLEE55 pKa = 4.07QNVGGGATQTGTATLQVAEE74 pKa = 4.64GGQYY78 pKa = 10.47SLQVALCNDD87 pKa = 4.41DD88 pKa = 4.0GCSTSAATDD97 pKa = 3.26IVVADD102 pKa = 4.05TDD104 pKa = 4.39GSHH107 pKa = 7.45LDD109 pKa = 4.54PITVTAGEE117 pKa = 4.14NNKK120 pKa = 10.15PYY122 pKa = 10.37TNSTNSVVGTYY133 pKa = 8.99FVEE136 pKa = 3.68WGVYY140 pKa = 7.95GRR142 pKa = 11.84KK143 pKa = 9.56FSVDD147 pKa = 3.16MMPSYY152 pKa = 11.49NLTHH156 pKa = 7.4LIYY159 pKa = 10.65GFIPICGGDD168 pKa = 4.17GINDD172 pKa = 3.6SLKK175 pKa = 10.38EE176 pKa = 4.02IEE178 pKa = 5.01GSFQALQRR186 pKa = 11.84SCSGRR191 pKa = 11.84EE192 pKa = 3.78DD193 pKa = 3.97FKK195 pKa = 11.81VSLHH199 pKa = 6.9DD200 pKa = 4.64PFAALQKK207 pKa = 10.36SQADD211 pKa = 3.6QTFSDD216 pKa = 4.67PYY218 pKa = 10.38KK219 pKa = 11.14GNFGQLMALKK229 pKa = 9.89QAYY232 pKa = 7.94PDD234 pKa = 4.18LKK236 pKa = 10.08ILPSIGGWTLSDD248 pKa = 3.3PFYY251 pKa = 10.79FFSDD255 pKa = 3.0AAKK258 pKa = 10.23RR259 pKa = 11.84KK260 pKa = 7.43TFVDD264 pKa = 3.65SVEE267 pKa = 3.9EE268 pKa = 5.27FIRR271 pKa = 11.84TWKK274 pKa = 10.55FFDD277 pKa = 4.25GVDD280 pKa = 3.86IDD282 pKa = 3.61WEE284 pKa = 4.58YY285 pKa = 11.03PGGQGANPTLGDD297 pKa = 3.6PAIDD301 pKa = 3.78GEE303 pKa = 4.65TYY305 pKa = 10.53RR306 pKa = 11.84LLMRR310 pKa = 11.84DD311 pKa = 3.73LRR313 pKa = 11.84AMLDD317 pKa = 3.43NLEE320 pKa = 4.19QEE322 pKa = 4.27TGRR325 pKa = 11.84TYY327 pKa = 11.39EE328 pKa = 3.95LTSAIGAGSDD338 pKa = 3.35KK339 pKa = 11.1IEE341 pKa = 4.43DD342 pKa = 3.22VDD344 pKa = 4.04YY345 pKa = 11.67LDD347 pKa = 3.46VQQYY351 pKa = 8.23MDD353 pKa = 3.88YY354 pKa = 10.1FFVMTYY360 pKa = 10.69DD361 pKa = 5.02FYY363 pKa = 11.64GGWSNEE369 pKa = 4.11VLGHH373 pKa = 5.33QAALYY378 pKa = 9.93APAWKK383 pKa = 9.54PDD385 pKa = 3.36TDD387 pKa = 3.91YY388 pKa = 7.94TTHH391 pKa = 7.19NGIQNLLGLGVDD403 pKa = 3.51PGKK406 pKa = 10.7LVVGAAMYY414 pKa = 10.31GRR416 pKa = 11.84GWKK419 pKa = 9.8GVSGWTGNDD428 pKa = 2.9HH429 pKa = 5.69MTGTATGMVNGTWEE443 pKa = 4.75DD444 pKa = 3.8GVVDD448 pKa = 3.56YY449 pKa = 11.27RR450 pKa = 11.84DD451 pKa = 3.3IVSRR455 pKa = 11.84IATGEE460 pKa = 3.68WEE462 pKa = 4.35EE463 pKa = 4.98YY464 pKa = 10.27YY465 pKa = 10.94DD466 pKa = 3.96STAEE470 pKa = 3.55APYY473 pKa = 10.12IFKK476 pKa = 10.55PSTGDD481 pKa = 3.68LITYY485 pKa = 9.37DD486 pKa = 3.45DD487 pKa = 4.72HH488 pKa = 8.32RR489 pKa = 11.84SVMAKK494 pKa = 9.81GAYY497 pKa = 8.43VQSNNLAGLFSWEE510 pKa = 3.53IDD512 pKa = 3.3ADD514 pKa = 3.73NGDD517 pKa = 4.0IMNAMHH523 pKa = 7.08EE524 pKa = 4.59SLGHH528 pKa = 6.47GDD530 pKa = 3.46GFGNRR535 pKa = 11.84APVARR540 pKa = 11.84AGGDD544 pKa = 3.29QSVDD548 pKa = 3.06SGASVSLDD556 pKa = 2.81GSTSSDD562 pKa = 3.59LDD564 pKa = 3.84NDD566 pKa = 3.99PLTYY570 pKa = 10.51SWVQVSGTSVTLQNASSASASFTAPAVSADD600 pKa = 3.68EE601 pKa = 4.21EE602 pKa = 4.97LVFSLTVSDD611 pKa = 5.51GEE613 pKa = 4.85ASDD616 pKa = 4.02SDD618 pKa = 3.94QVSVTVVADD627 pKa = 3.81QPNQAPSADD636 pKa = 3.41AGADD640 pKa = 3.54QMVTTPATVTLNGSASSDD658 pKa = 3.57PDD660 pKa = 3.54GDD662 pKa = 3.82ALTYY666 pKa = 10.76SWVQVAGSSVTLSDD680 pKa = 3.97ASSATPSFSAAEE692 pKa = 4.04VSAEE696 pKa = 3.85QEE698 pKa = 4.16LVFEE702 pKa = 5.27LNVNDD707 pKa = 5.19GSLSDD712 pKa = 3.66ATPDD716 pKa = 3.3QVSIFLLPADD726 pKa = 4.78ANTPPQVSAPAQVTIQEE743 pKa = 4.46GASDD747 pKa = 4.76SITATGTDD755 pKa = 3.32ADD757 pKa = 4.5GDD759 pKa = 4.13ALTYY763 pKa = 8.45TWSGMVSGSGDD774 pKa = 3.98TISITAPQVEE784 pKa = 4.59ADD786 pKa = 3.84TNFTLTVTVSDD797 pKa = 5.52GIASASADD805 pKa = 3.31VTVTVTNVIVDD816 pKa = 4.25GGCSSTDD823 pKa = 3.37PNAGNVPAWQSGSTYY838 pKa = 10.67LGGDD842 pKa = 3.51QVSHH846 pKa = 6.2EE847 pKa = 4.04QLVWQAKK854 pKa = 8.35YY855 pKa = 7.14WTQQEE860 pKa = 4.49PGFSSADD867 pKa = 3.2WQLVSDD873 pKa = 5.78IEE875 pKa = 4.63VPWNASTAYY884 pKa = 10.63NGGDD888 pKa = 3.43EE889 pKa = 4.39VNHH892 pKa = 5.64EE893 pKa = 4.39GKK895 pKa = 10.49RR896 pKa = 11.84YY897 pKa = 8.33RR898 pKa = 11.84AKK900 pKa = 9.79WWTQGQNPSSSSDD913 pKa = 2.93WEE915 pKa = 4.37EE916 pKa = 5.01IGDD919 pKa = 3.92ASCNN923 pKa = 3.64

Molecular weight: 98.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2M9B5|A0A1Q2M9B5_9GAMM GntR family transcriptional regulator OS=Microbulbifer agarilyticus OX=260552 GN=Mag101_01875 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.2SGRR28 pKa = 11.84KK29 pKa = 8.27ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.19VLCAA44 pKa = 3.83

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3454

0

3454

1229174

38

7326

355.9

39.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.199 ± 0.052

0.976 ± 0.014

5.857 ± 0.047

6.455 ± 0.041

3.893 ± 0.027

7.939 ± 0.045

2.101 ± 0.022

5.095 ± 0.026

3.879 ± 0.039

10.223 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.02

3.636 ± 0.032

4.53 ± 0.038

4.266 ± 0.032

5.786 ± 0.053

6.313 ± 0.047

5.129 ± 0.039

7.124 ± 0.038

1.421 ± 0.017

2.874 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski