Freshwater phage uvFW-CGR-AMD-COM-C455

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XUE8|A0A1B0XUE8_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C455 OX=1838156 GN=uvFWCGRAMDCOMC455_042 PE=4 SV=1
MM1 pKa = 7.67TEE3 pKa = 3.83TPDD6 pKa = 2.87WRR8 pKa = 11.84GIPTAACPTCGGVWFNVPVIFDD30 pKa = 3.81PEE32 pKa = 4.4TYY34 pKa = 10.31EE35 pKa = 3.75PAGWGTEE42 pKa = 4.11ATCFSCDD49 pKa = 3.48TLVTAPCPLDD59 pKa = 3.82LPNAILL65 pKa = 4.16

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XUF8|A0A1B0XUF8_9CAUD Transcription factor WhiB OS=Freshwater phage uvFW-CGR-AMD-COM-C455 OX=1838156 GN=uvFWCGRAMDCOMC455_08 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 6.24EE3 pKa = 4.08ILEE6 pKa = 4.16LEE8 pKa = 4.42IYY10 pKa = 10.66SNGWLDD16 pKa = 4.34FGLAGQLYY24 pKa = 10.6VGIDD28 pKa = 3.74LKK30 pKa = 11.36AVIVVVLAVATIKK43 pKa = 10.77FIAPRR48 pKa = 11.84IRR50 pKa = 11.84RR51 pKa = 11.84TRR53 pKa = 11.84SRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 3.21

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11382

40

1737

196.2

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.085 ± 0.423

0.756 ± 0.205

5.737 ± 0.31

6.431 ± 0.47

3.453 ± 0.227

7.327 ± 0.352

1.45 ± 0.242

6.018 ± 0.182

6.238 ± 0.435

8.004 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.144 ± 0.286

5.105 ± 0.308

4.252 ± 0.251

3.831 ± 0.256

4.771 ± 0.274

6.774 ± 0.402

7.152 ± 0.715

6.273 ± 0.26

1.819 ± 0.224

3.383 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski