Amino acid dipepetide frequency for Oceanobacillus piezotolerans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.471AlaAla: 5.471 ± 0.087	0.538AlaCys: 0.538 ± 0.022	3.35AlaAsp: 3.35 ± 0.06	4.718AlaGlu: 4.718 ± 0.083	3.391AlaPhe: 3.391 ± 0.065	5.124AlaGly: 5.124 ± 0.084	1.177AlaHis: 1.177 ± 0.033	6.25AlaIle: 6.25 ± 0.085	4.417AlaLys: 4.417 ± 0.057	6.867AlaLeu: 6.867 ± 0.101	2.018AlaMet: 2.018 ± 0.046	2.955AlaAsn: 2.955 ± 0.068	1.969AlaPro: 1.969 ± 0.048	1.963AlaGln: 1.963 ± 0.044	2.511AlaArg: 2.511 ± 0.043	4.041AlaSer: 4.041 ± 0.071	3.415AlaThr: 3.415 ± 0.057	5.148AlaVal: 5.148 ± 0.071	0.642AlaTrp: 0.642 ± 0.024	2.384AlaTyr: 2.384 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.34CysAla: 0.34 ± 0.02	0.071CysCys: 0.071 ± 0.008	0.34CysAsp: 0.34 ± 0.019	0.348CysGlu: 0.348 ± 0.02	0.268CysPhe: 0.268 ± 0.014	0.58CysGly: 0.58 ± 0.027	0.181CysHis: 0.181 ± 0.012	0.47CysIle: 0.47 ± 0.024	0.324CysLys: 0.324 ± 0.017	0.548CysLeu: 0.548 ± 0.021	0.143CysMet: 0.143 ± 0.013	0.277CysAsn: 0.277 ± 0.014	0.28CysPro: 0.28 ± 0.018	0.192CysGln: 0.192 ± 0.013	0.254CysArg: 0.254 ± 0.016	0.425CysSer: 0.425 ± 0.018	0.36CysThr: 0.36 ± 0.017	0.343CysVal: 0.343 ± 0.019	0.052CysTrp: 0.052 ± 0.006	0.216CysTyr: 0.216 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.246AspAla: 3.246 ± 0.054	0.325AspCys: 0.325 ± 0.017	2.448AspAsp: 2.448 ± 0.05	4.406AspGlu: 4.406 ± 0.079	2.498AspPhe: 2.498 ± 0.049	3.394AspGly: 3.394 ± 0.066	1.105AspHis: 1.105 ± 0.037	4.5AspIle: 4.5 ± 0.071	3.41AspLys: 3.41 ± 0.053	4.895AspLeu: 4.895 ± 0.073	1.401AspMet: 1.401 ± 0.035	2.181AspAsn: 2.181 ± 0.054	1.951AspPro: 1.951 ± 0.05	1.724AspGln: 1.724 ± 0.045	2.18AspArg: 2.18 ± 0.049	2.758AspSer: 2.758 ± 0.048	2.594AspThr: 2.594 ± 0.061	3.719AspVal: 3.719 ± 0.064	0.604AspTrp: 0.604 ± 0.025	2.387AspTyr: 2.387 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	5.667GluAla: 5.667 ± 0.079	0.305GluCys: 0.305 ± 0.017	4.163GluAsp: 4.163 ± 0.073	8.042GluGlu: 8.042 ± 0.124	2.594GluPhe: 2.594 ± 0.049	4.472GluGly: 4.472 ± 0.065	1.45GluHis: 1.45 ± 0.033	6.245GluIle: 6.245 ± 0.079	6.789GluLys: 6.789 ± 0.091	7.283GluLeu: 7.283 ± 0.082	2.283GluMet: 2.283 ± 0.052	4.145GluAsn: 4.145 ± 0.075	2.06GluPro: 2.06 ± 0.048	3.359GluGln: 3.359 ± 0.057	3.474GluArg: 3.474 ± 0.059	3.822GluSer: 3.822 ± 0.063	3.98GluThr: 3.98 ± 0.066	5.516GluVal: 5.516 ± 0.08	0.819GluTrp: 0.819 ± 0.025	2.379GluTyr: 2.379 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.952PheAla: 2.952 ± 0.059	0.297PheCys: 0.297 ± 0.017	2.274PheAsp: 2.274 ± 0.043	2.796PheGlu: 2.796 ± 0.048	2.273PhePhe: 2.273 ± 0.057	3.336PheGly: 3.336 ± 0.073	1.044PheHis: 1.044 ± 0.04	4.265PheIle: 4.265 ± 0.079	2.283PheLys: 2.283 ± 0.043	4.468PheLeu: 4.468 ± 0.074	1.169PheMet: 1.169 ± 0.036	1.999PheAsn: 1.999 ± 0.043	1.667PhePro: 1.667 ± 0.039	1.659PheGln: 1.659 ± 0.044	1.519PheArg: 1.519 ± 0.035	3.227PheSer: 3.227 ± 0.055	2.573PheThr: 2.573 ± 0.046	3.096PheVal: 3.096 ± 0.056	0.481PheTrp: 0.481 ± 0.024	1.786PheTyr: 1.786 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.788GlyAla: 4.788 ± 0.074	0.5GlyCys: 0.5 ± 0.023	3.242GlyAsp: 3.242 ± 0.062	4.835GlyGlu: 4.835 ± 0.07	3.381GlyPhe: 3.381 ± 0.064	4.691GlyGly: 4.691 ± 0.084	1.359GlyHis: 1.359 ± 0.038	6.246GlyIle: 6.246 ± 0.079	4.848GlyLys: 4.848 ± 0.072	6.515GlyLeu: 6.515 ± 0.086	2.135GlyMet: 2.135 ± 0.046	2.969GlyAsn: 2.969 ± 0.062	1.754GlyPro: 1.754 ± 0.044	2.037GlyGln: 2.037 ± 0.048	2.584GlyArg: 2.584 ± 0.055	3.87GlySer: 3.87 ± 0.066	3.997GlyThr: 3.997 ± 0.062	4.958GlyVal: 4.958 ± 0.073	0.774GlyTrp: 0.774 ± 0.029	2.88GlyTyr: 2.88 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.352HisAla: 1.352 ± 0.037	0.182HisCys: 0.182 ± 0.015	0.991HisAsp: 0.991 ± 0.033	1.332HisGlu: 1.332 ± 0.035	1.04HisPhe: 1.04 ± 0.033	1.425HisGly: 1.425 ± 0.044	0.72HisHis: 0.72 ± 0.034	1.593HisIle: 1.593 ± 0.042	0.99HisLys: 0.99 ± 0.034	2.056HisLeu: 2.056 ± 0.045	0.499HisMet: 0.499 ± 0.023	0.813HisAsn: 0.813 ± 0.03	1.12HisPro: 1.12 ± 0.032	0.853HisGln: 0.853 ± 0.029	0.845HisArg: 0.845 ± 0.03	1.221HisSer: 1.221 ± 0.036	1.092HisThr: 1.092 ± 0.034	1.402HisVal: 1.402 ± 0.038	0.218HisTrp: 0.218 ± 0.015	0.905HisTyr: 0.905 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.322IleAla: 6.322 ± 0.088	0.572IleCys: 0.572 ± 0.022	4.455IleAsp: 4.455 ± 0.075	6.014IleGlu: 6.014 ± 0.077	3.562IlePhe: 3.562 ± 0.058	6.412IleGly: 6.412 ± 0.09	1.829IleHis: 1.829 ± 0.045	7.027IleIle: 7.027 ± 0.104	4.699IleLys: 4.699 ± 0.082	7.765IleLeu: 7.765 ± 0.096	1.959IleMet: 1.959 ± 0.046	3.753IleAsn: 3.753 ± 0.062	3.649IlePro: 3.649 ± 0.06	3.144IleGln: 3.144 ± 0.058	3.223IleArg: 3.223 ± 0.062	5.395IleSer: 5.395 ± 0.081	4.729IleThr: 4.729 ± 0.073	5.63IleVal: 5.63 ± 0.086	0.693IleTrp: 0.693 ± 0.029	2.699IleTyr: 2.699 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.426LysAla: 4.426 ± 0.071	0.264LysCys: 0.264 ± 0.016	3.966LysAsp: 3.966 ± 0.059	7.354LysGlu: 7.354 ± 0.1	1.874LysPhe: 1.874 ± 0.038	4.273LysGly: 4.273 ± 0.063	1.377LysHis: 1.377 ± 0.037	4.436LysIle: 4.436 ± 0.064	5.377LysLys: 5.377 ± 0.086	5.775LysLeu: 5.775 ± 0.078	2.063LysMet: 2.063 ± 0.04	3.272LysAsn: 3.272 ± 0.052	2.122LysPro: 2.122 ± 0.044	3.13LysGln: 3.13 ± 0.062	3.147LysArg: 3.147 ± 0.059	3.378LysSer: 3.378 ± 0.058	3.305LysThr: 3.305 ± 0.055	4.543LysVal: 4.543 ± 0.067	0.763LysTrp: 0.763 ± 0.027	2.164LysTyr: 2.164 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	6.912LeuAla: 6.912 ± 0.082	0.544LeuCys: 0.544 ± 0.026	4.938LeuAsp: 4.938 ± 0.071	7.131LeuGlu: 7.131 ± 0.084	4.81LeuPhe: 4.81 ± 0.086	6.444LeuGly: 6.444 ± 0.085	1.854LeuHis: 1.854 ± 0.04	7.652LeuIle: 7.652 ± 0.1	6.264LeuLys: 6.264 ± 0.082	9.897LeuLeu: 9.897 ± 0.115	2.497LeuMet: 2.497 ± 0.046	4.392LeuAsn: 4.392 ± 0.071	3.748LeuPro: 3.748 ± 0.06	3.463LeuGln: 3.463 ± 0.055	3.506LeuArg: 3.506 ± 0.063	6.542LeuSer: 6.542 ± 0.077	5.451LeuThr: 5.451 ± 0.069	6.212LeuVal: 6.212 ± 0.08	0.758LeuTrp: 0.758 ± 0.03	3.147LeuTyr: 3.147 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	1.914MetAla: 1.914 ± 0.045	0.108MetCys: 0.108 ± 0.009	1.648MetAsp: 1.648 ± 0.044	2.332MetGlu: 2.332 ± 0.046	1.011MetPhe: 1.011 ± 0.033	1.748MetGly: 1.748 ± 0.037	0.462MetHis: 0.462 ± 0.02	2.207MetIle: 2.207 ± 0.045	2.431MetLys: 2.431 ± 0.045	2.461MetLeu: 2.461 ± 0.049	0.817MetMet: 0.817 ± 0.029	1.61MetAsn: 1.61 ± 0.042	0.954MetPro: 0.954 ± 0.034	0.945MetGln: 0.945 ± 0.033	1.04MetArg: 1.04 ± 0.03	1.584MetSer: 1.584 ± 0.039	1.549MetThr: 1.549 ± 0.037	1.843MetVal: 1.843 ± 0.036	0.181MetTrp: 0.181 ± 0.014	0.764MetTyr: 0.764 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.829AsnAla: 2.829 ± 0.053	0.273AsnCys: 0.273 ± 0.016	2.34AsnAsp: 2.34 ± 0.048	3.775AsnGlu: 3.775 ± 0.062	1.688AsnPhe: 1.688 ± 0.038	3.462AsnGly: 3.462 ± 0.064	1.173AsnHis: 1.173 ± 0.038	3.719AsnIle: 3.719 ± 0.069	3.321AsnLys: 3.321 ± 0.059	4.021AsnLeu: 4.021 ± 0.062	1.294AsnMet: 1.294 ± 0.038	2.405AsnAsn: 2.405 ± 0.056	2.312AsnPro: 2.312 ± 0.052	2.099AsnGln: 2.099 ± 0.051	2.192AsnArg: 2.192 ± 0.047	2.418AsnSer: 2.418 ± 0.057	2.285AsnThr: 2.285 ± 0.051	3.148AsnVal: 3.148 ± 0.063	0.572AsnTrp: 0.572 ± 0.021	1.78AsnTyr: 1.78 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.167ProAla: 2.167 ± 0.051	0.17ProCys: 0.17 ± 0.013	2.038ProAsp: 2.038 ± 0.05	3.136ProGlu: 3.136 ± 0.056	2.012ProPhe: 2.012 ± 0.046	2.285ProGly: 2.285 ± 0.062	0.727ProHis: 0.727 ± 0.032	3.094ProIle: 3.094 ± 0.044	2.133ProLys: 2.133 ± 0.049	3.431ProLeu: 3.431 ± 0.062	0.866ProMet: 0.866 ± 0.028	1.862ProAsn: 1.862 ± 0.044	0.906ProPro: 0.906 ± 0.032	0.97ProGln: 0.97 ± 0.033	1.042ProArg: 1.042 ± 0.03	2.301ProSer: 2.301 ± 0.046	1.979ProThr: 1.979 ± 0.043	2.724ProVal: 2.724 ± 0.055	0.359ProTrp: 0.359 ± 0.019	1.413ProTyr: 1.413 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	2.76GlnAla: 2.76 ± 0.05	0.166GlnCys: 0.166 ± 0.012	1.733GlnAsp: 1.733 ± 0.044	3.009GlnGlu: 3.009 ± 0.053	1.582GlnPhe: 1.582 ± 0.033	2.199GlnGly: 2.199 ± 0.05	0.678GlnHis: 0.678 ± 0.027	2.739GlnIle: 2.739 ± 0.051	2.37GlnLys: 2.37 ± 0.047	3.903GlnLeu: 3.903 ± 0.078	1.068GlnMet: 1.068 ± 0.036	1.548GlnAsn: 1.548 ± 0.04	1.122GlnPro: 1.122 ± 0.034	1.676GlnGln: 1.676 ± 0.047	1.453GlnArg: 1.453 ± 0.04	2.034GlnSer: 2.034 ± 0.047	1.952GlnThr: 1.952 ± 0.045	2.433GlnVal: 2.433 ± 0.041	0.391GlnTrp: 0.391 ± 0.021	1.316GlnTyr: 1.316 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.324ArgAla: 2.324 ± 0.047	0.215ArgCys: 0.215 ± 0.017	2.081ArgAsp: 2.081 ± 0.042	3.282ArgGlu: 3.282 ± 0.072	1.812ArgPhe: 1.812 ± 0.04	2.231ArgGly: 2.231 ± 0.045	0.702ArgHis: 0.702 ± 0.025	3.24ArgIle: 3.24 ± 0.054	3.168ArgLys: 3.168 ± 0.058	3.819ArgLeu: 3.819 ± 0.059	1.271ArgMet: 1.271 ± 0.034	2.066ArgAsn: 2.066 ± 0.049	1.18ArgPro: 1.18 ± 0.038	1.376ArgGln: 1.376 ± 0.037	1.691ArgArg: 1.691 ± 0.045	2.123ArgSer: 2.123 ± 0.044	2.058ArgThr: 2.058 ± 0.044	2.646ArgVal: 2.646 ± 0.051	0.372ArgTrp: 0.372 ± 0.019	1.526ArgTyr: 1.526 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.468SerAla: 3.468 ± 0.059	0.326SerCys: 0.326 ± 0.017	2.9SerAsp: 2.9 ± 0.058	3.979SerGlu: 3.979 ± 0.067	3.208SerPhe: 3.208 ± 0.047	4.339SerGly: 4.339 ± 0.066	1.29SerHis: 1.29 ± 0.037	5.395SerIle: 5.395 ± 0.082	3.8SerLys: 3.8 ± 0.056	5.962SerLeu: 5.962 ± 0.074	1.716SerMet: 1.716 ± 0.044	2.8SerAsn: 2.8 ± 0.049	2.096SerPro: 2.096 ± 0.046	1.806SerGln: 1.806 ± 0.04	2.232SerArg: 2.232 ± 0.049	3.769SerSer: 3.769 ± 0.068	3.155SerThr: 3.155 ± 0.054	4.057SerVal: 4.057 ± 0.057	0.617SerTrp: 0.617 ± 0.027	2.366SerTyr: 2.366 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	3.887ThrAla: 3.887 ± 0.064	0.334ThrCys: 0.334 ± 0.017	2.692ThrAsp: 2.692 ± 0.051	3.871ThrGlu: 3.871 ± 0.064	2.597ThrPhe: 2.597 ± 0.057	4.09ThrGly: 4.09 ± 0.06	1.018ThrHis: 1.018 ± 0.025	4.848ThrIle: 4.848 ± 0.078	3.212ThrLys: 3.212 ± 0.057	5.158ThrLeu: 5.158 ± 0.071	1.329ThrMet: 1.329 ± 0.035	2.63ThrAsn: 2.63 ± 0.054	2.26ThrPro: 2.26 ± 0.048	1.38ThrGln: 1.38 ± 0.036	1.765ThrArg: 1.765 ± 0.035	3.322ThrSer: 3.322 ± 0.049	2.911ThrThr: 2.911 ± 0.055	4.075ThrVal: 4.075 ± 0.056	0.537ThrTrp: 0.537 ± 0.024	1.973ThrTyr: 1.973 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	4.76ValAla: 4.76 ± 0.07	0.475ValCys: 0.475 ± 0.02	3.671ValAsp: 3.671 ± 0.061	5.171ValGlu: 5.171 ± 0.069	3.183ValPhe: 3.183 ± 0.059	4.619ValGly: 4.619 ± 0.069	1.372ValHis: 1.372 ± 0.036	6.05ValIle: 6.05 ± 0.086	4.388ValLys: 4.388 ± 0.055	6.743ValLeu: 6.743 ± 0.076	1.896ValMet: 1.896 ± 0.045	3.251ValAsn: 3.251 ± 0.055	2.71ValPro: 2.71 ± 0.051	2.274ValGln: 2.274 ± 0.046	2.49ValArg: 2.49 ± 0.049	4.32ValSer: 4.32 ± 0.066	4.069ValThr: 4.069 ± 0.067	4.86ValVal: 4.86 ± 0.071	0.569ValTrp: 0.569 ± 0.025	2.305ValTyr: 2.305 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.582TrpAla: 0.582 ± 0.023	0.069TrpCys: 0.069 ± 0.008	0.491TrpAsp: 0.491 ± 0.023	0.695TrpGlu: 0.695 ± 0.03	0.541TrpPhe: 0.541 ± 0.025	0.709TrpGly: 0.709 ± 0.029	0.177TrpHis: 0.177 ± 0.012	0.874TrpIle: 0.874 ± 0.029	0.73TrpLys: 0.73 ± 0.028	1.032TrpLeu: 1.032 ± 0.035	0.322TrpMet: 0.322 ± 0.016	0.574TrpAsn: 0.574 ± 0.022	0.259TrpPro: 0.259 ± 0.016	0.375TrpGln: 0.375 ± 0.02	0.373TrpArg: 0.373 ± 0.019	0.546TrpSer: 0.546 ± 0.023	0.484TrpThr: 0.484 ± 0.018	0.637TrpVal: 0.637 ± 0.024	0.144TrpTrp: 0.144 ± 0.013	0.346TrpTyr: 0.346 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.182TyrAla: 2.182 ± 0.046	0.278TyrCys: 0.278 ± 0.016	1.965TyrAsp: 1.965 ± 0.048	2.623TyrGlu: 2.623 ± 0.055	1.841TyrPhe: 1.841 ± 0.04	2.529TyrGly: 2.529 ± 0.046	0.955TyrHis: 0.955 ± 0.032	2.683TyrIle: 2.683 ± 0.055	2.056TyrLys: 2.056 ± 0.04	3.563TyrLeu: 3.563 ± 0.059	0.86TyrMet: 0.86 ± 0.029	1.606TyrAsn: 1.606 ± 0.038	1.494TyrPro: 1.494 ± 0.035	1.707TyrGln: 1.707 ± 0.041	1.661TyrArg: 1.661 ± 0.041	2.185TyrSer: 2.185 ± 0.053	1.951TyrThr: 1.951 ± 0.045	2.186TyrVal: 2.186 ± 0.044	0.413TyrTrp: 0.413 ± 0.019	1.499TyrTyr: 1.499 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3815 proteins (1080255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---