Streptococcus phage Javan129

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZR6|A0A4D5ZZR6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan129 OX=2547994 GN=Javan129_0035 PE=4 SV=1
MM1 pKa = 7.06QCADD5 pKa = 4.25LPCFIVKK12 pKa = 9.41NWFGIDD18 pKa = 3.08LWGNAIDD25 pKa = 4.76LLNSASAQGLEE36 pKa = 4.45VIYY39 pKa = 10.04NAPGVNPKK47 pKa = 10.68ASDD50 pKa = 3.69LFVMEE55 pKa = 4.66VAGSPYY61 pKa = 10.04GHH63 pKa = 6.88TGAVIEE69 pKa = 5.19DD70 pKa = 3.5SDD72 pKa = 4.68GYY74 pKa = 8.88TIKK77 pKa = 9.94TVEE80 pKa = 4.02QNIDD84 pKa = 3.8GNWDD88 pKa = 3.49SLQVGGPARR97 pKa = 11.84FNTRR101 pKa = 11.84DD102 pKa = 3.46FTGVVGWIRR111 pKa = 11.84LPVDD115 pKa = 3.27HH116 pKa = 7.17TNQTVDD122 pKa = 3.59TAPQTSDD129 pKa = 3.59TIVEE133 pKa = 4.31TSKK136 pKa = 11.12SGTFTLDD143 pKa = 2.86VAEE146 pKa = 4.14INIRR150 pKa = 11.84RR151 pKa = 11.84WPSLASEE158 pKa = 4.53VVGSYY163 pKa = 10.28KK164 pKa = 10.7QGDD167 pKa = 3.97TVSFDD172 pKa = 3.52SEE174 pKa = 5.0GYY176 pKa = 10.18ANGYY180 pKa = 8.37YY181 pKa = 9.51WISYY185 pKa = 9.21VGGSGMRR192 pKa = 11.84NYY194 pKa = 9.35MAIGITDD201 pKa = 3.47KK202 pKa = 11.18DD203 pKa = 3.91GNIISLWGKK212 pKa = 9.69LVV214 pKa = 3.14

Molecular weight: 23.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZZM6|A0A4D5ZZM6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan129 OX=2547994 GN=Javan129_0008 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.38KK3 pKa = 10.43RR4 pKa = 11.84PLSLLTTSKK13 pKa = 10.77VATLGFGLAVNMSIGTVLSNLDD35 pKa = 2.84IDD37 pKa = 4.16LALVRR42 pKa = 11.84IVKK45 pKa = 10.02RR46 pKa = 11.84RR47 pKa = 11.84CC48 pKa = 2.94

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12268

42

1307

191.7

21.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.839 ± 0.634

0.538 ± 0.117

6.741 ± 0.332

7.336 ± 0.299

3.635 ± 0.188

6.276 ± 0.334

1.451 ± 0.154

6.92 ± 0.294

9.129 ± 0.307

9.032 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.16

5.722 ± 0.228

2.771 ± 0.186

4.141 ± 0.181

4.051 ± 0.197

6.065 ± 0.315

6.097 ± 0.236

6.122 ± 0.27

1.06 ± 0.095

3.741 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski