Streptococcus phage phi-SsuFJNP8_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MMM1|A0A7G3MMM1_9CAUD Mobile element protein OS=Streptococcus phage phi-SsuFJNP8_rum OX=2664391 PE=4 SV=1
MM1 pKa = 8.08SEE3 pKa = 3.56ITMLPLGSVVILKK16 pKa = 10.86GNTKK20 pKa = 10.59KK21 pKa = 10.43MMIISRR27 pKa = 11.84VIAAPVKK34 pKa = 9.58GEE36 pKa = 4.02VYY38 pKa = 10.45RR39 pKa = 11.84FDD41 pKa = 4.36YY42 pKa = 10.35GACLYY47 pKa = 10.35PEE49 pKa = 4.55GLMGDD54 pKa = 3.41SLIYY58 pKa = 10.45FNHH61 pKa = 6.23EE62 pKa = 4.64DD63 pKa = 3.19ILKK66 pKa = 9.78VVQEE70 pKa = 5.21GYY72 pKa = 9.38TDD74 pKa = 4.24DD75 pKa = 5.16DD76 pKa = 4.25NEE78 pKa = 4.28IMLEE82 pKa = 4.12NIANILATTDD92 pKa = 3.08IPKK95 pKa = 10.81GDD97 pKa = 3.49VATLNEE103 pKa = 4.1VNGIGGG109 pKa = 3.5

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MJU1|A0A7G3MJU1_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJNP8_rum OX=2664391 PE=4 SV=1
MM1 pKa = 8.01LYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.21DD29 pKa = 3.63IRR31 pKa = 11.84NEE33 pKa = 3.56FEE35 pKa = 3.95RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.16RR40 pKa = 11.84KK41 pKa = 9.86KK42 pKa = 10.1EE43 pKa = 3.91NLSQTPLSRR52 pKa = 11.84KK53 pKa = 9.46NKK55 pKa = 9.68

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

23209

41

1493

283.0

32.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.407 ± 0.33

0.732 ± 0.107

5.774 ± 0.22

7.48 ± 0.277

3.938 ± 0.163

6.17 ± 0.216

1.935 ± 0.121

6.812 ± 0.238

7.234 ± 0.253

9.621 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.121

5.024 ± 0.163

2.947 ± 0.155

4.537 ± 0.186

4.3 ± 0.179

7.096 ± 0.323

6.217 ± 0.313

6.299 ± 0.149

1.168 ± 0.077

4.059 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski