Bradyrhizobium sp. LMTR 3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9YI77|A0A1B9YI77_9BRAD Uncharacterized protein OS=Bradyrhizobium sp. LMTR 3 OX=189873 GN=LMTR3_26510 PE=4 SV=1
MM1 pKa = 7.6ASASFVEE8 pKa = 5.22IISHH12 pKa = 6.11FAGYY16 pKa = 7.91MQIFEE21 pKa = 5.46DD22 pKa = 3.51IARR25 pKa = 11.84DD26 pKa = 3.81RR27 pKa = 11.84IQYY30 pKa = 10.68DD31 pKa = 3.65EE32 pKa = 4.53TPGPGPSGDD41 pKa = 3.72YY42 pKa = 7.55TTLRR46 pKa = 11.84PNYY49 pKa = 7.8HH50 pKa = 6.31HH51 pKa = 7.59RR52 pKa = 11.84YY53 pKa = 9.44APEE56 pKa = 5.37DD57 pKa = 3.7MDD59 pKa = 4.46SAASPVPEE67 pKa = 4.89VIADD71 pKa = 3.94DD72 pKa = 4.36PMDD75 pKa = 4.52MIRR78 pKa = 11.84GRR80 pKa = 11.84PLKK83 pKa = 10.16LLKK86 pKa = 10.6GPQDD90 pKa = 3.38PDD92 pKa = 3.32FDD94 pKa = 4.83FFPTPPKK101 pKa = 10.22PNIVLPPGGGGGGGGGSEE119 pKa = 3.86FNIRR123 pKa = 11.84VKK125 pKa = 11.23YY126 pKa = 9.83EE127 pKa = 4.07DD128 pKa = 3.98GGAEE132 pKa = 3.79IQLTVHH138 pKa = 5.31QHH140 pKa = 3.86NFMRR144 pKa = 11.84DD145 pKa = 2.87EE146 pKa = 4.63DD147 pKa = 4.58FNLPADD153 pKa = 4.06FLVAAEE159 pKa = 4.21PLIMSLNSDD168 pKa = 3.06AMATIEE174 pKa = 4.1QLVANASAEE183 pKa = 4.07IPTDD187 pKa = 1.98WWMPQNDD194 pKa = 3.63AGATDD199 pKa = 4.2FLITHH204 pKa = 7.05DD205 pKa = 4.53AAWADD210 pKa = 3.84RR211 pKa = 11.84DD212 pKa = 4.01GTPDD216 pKa = 3.32EE217 pKa = 4.8HH218 pKa = 7.28SVPAGYY224 pKa = 9.91YY225 pKa = 10.41VNGEE229 pKa = 4.02LQEE232 pKa = 4.7PPTEE236 pKa = 3.94PRR238 pKa = 11.84PPIEE242 pKa = 4.85LKK244 pKa = 10.44EE245 pKa = 4.17LPDD248 pKa = 3.66TGDD251 pKa = 5.66GIGQWASMGGNYY263 pKa = 10.09SINAALLVDD272 pKa = 3.71IGEE275 pKa = 4.56SARR278 pKa = 11.84TMVVLGDD285 pKa = 3.84YY286 pKa = 10.13FKK288 pKa = 10.81TDD290 pKa = 4.33AMFQTNTTVDD300 pKa = 3.44NDD302 pKa = 3.7EE303 pKa = 4.33VSVSGGEE310 pKa = 4.11GTPSVTSEE318 pKa = 4.08GNVATNIANFVQNPSIYY335 pKa = 10.71ADD337 pKa = 3.98LPSYY341 pKa = 9.26WAGPNWIVDD350 pKa = 4.03VVDD353 pKa = 3.42GDD355 pKa = 4.29YY356 pKa = 11.54YY357 pKa = 11.46SVNTVSQTNYY367 pKa = 10.65LSDD370 pKa = 3.78NDD372 pKa = 3.5VATQVSSEE380 pKa = 3.66GHH382 pKa = 5.73YY383 pKa = 11.1NLVSGHH389 pKa = 5.57NQLGNLAQIFDD400 pKa = 4.28GEE402 pKa = 4.1IHH404 pKa = 6.77YY405 pKa = 10.67DD406 pKa = 3.86LIIIEE411 pKa = 4.29GAYY414 pKa = 10.44HH415 pKa = 6.21GMNVIFQNNILLNNDD430 pKa = 4.32NIVMSADD437 pKa = 3.33GTDD440 pKa = 3.56PSQSVNSGHH449 pKa = 6.67NSLLNEE455 pKa = 4.23GAIEE459 pKa = 4.19NYY461 pKa = 10.64GGDD464 pKa = 3.79TFDD467 pKa = 4.38GLTPGLQQILDD478 pKa = 4.06LLASGVTSIDD488 pKa = 3.58PEE490 pKa = 4.58LGSIIAGNGGPLRR503 pKa = 11.84VLYY506 pKa = 10.9VKK508 pKa = 10.74GDD510 pKa = 3.7YY511 pKa = 11.02YY512 pKa = 11.0DD513 pKa = 5.09INAVWQTNITSDD525 pKa = 3.92INVLYY530 pKa = 10.27QLQNEE535 pKa = 4.49PSPDD539 pKa = 3.78LMALDD544 pKa = 4.59PDD546 pKa = 4.23ADD548 pKa = 4.11VTQSASTGGNEE559 pKa = 4.48LANDD563 pKa = 3.85AAIVDD568 pKa = 4.0VNPDD572 pKa = 3.36VIYY575 pKa = 11.25VEE577 pKa = 4.66GEE579 pKa = 4.27VYY581 pKa = 10.17TDD583 pKa = 4.2SILVQANLLPVDD595 pKa = 3.76QDD597 pKa = 3.73DD598 pKa = 4.69AVNGDD603 pKa = 3.39TDD605 pKa = 3.87TLVTEE610 pKa = 5.62LIAFVDD616 pKa = 3.96DD617 pKa = 4.38TQDD620 pKa = 3.18QTNASPAVVTNSVQADD636 pKa = 3.57PMASVLHH643 pKa = 6.19

Molecular weight: 69.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9Z3M2|A0A1B9Z3M2_9BRAD Probable GTP-binding protein EngB OS=Bradyrhizobium sp. LMTR 3 OX=189873 GN=engB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6979

0

6979

2128772

29

5480

305.0

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.364 ± 0.044

0.88 ± 0.01

5.437 ± 0.026

5.428 ± 0.028

3.779 ± 0.021

8.297 ± 0.032

2.038 ± 0.015

5.429 ± 0.021

3.74 ± 0.029

9.869 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.015

2.791 ± 0.02

5.25 ± 0.026

3.175 ± 0.016

7.008 ± 0.033

5.65 ± 0.022

5.312 ± 0.027

7.456 ± 0.025

1.339 ± 0.014

2.269 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski