Vaccinia virus (strain Tian Tan) (VACV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77TF4|Q77TF4_VACCT Uncharacterized protein OS=Vaccinia virus (strain Tian Tan) OX=10253 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84LPILLLLISLVYY15 pKa = 9.16STPSPQTSKK24 pKa = 11.19KK25 pKa = 10.19IGDD28 pKa = 4.3DD29 pKa = 3.19ATLSCNRR36 pKa = 11.84NNTNDD41 pKa = 3.96YY42 pKa = 10.9VVMSAWYY49 pKa = 9.51KK50 pKa = 9.87EE51 pKa = 4.05PNSIILLAAKK61 pKa = 10.26SDD63 pKa = 3.41VLYY66 pKa = 10.72FDD68 pKa = 5.73NYY70 pKa = 9.77TKK72 pKa = 11.04DD73 pKa = 3.41KK74 pKa = 10.63ISYY77 pKa = 9.41DD78 pKa = 3.49SPYY81 pKa = 10.83DD82 pKa = 3.76DD83 pKa = 5.42LVTTITIKK91 pKa = 10.91SLTARR96 pKa = 11.84DD97 pKa = 3.35AGTYY101 pKa = 9.33VCAFFMTSPTNDD113 pKa = 3.34TDD115 pKa = 3.72KK116 pKa = 11.3VDD118 pKa = 3.61YY119 pKa = 10.85EE120 pKa = 4.4EE121 pKa = 5.24YY122 pKa = 9.3STEE125 pKa = 4.64LIVNTDD131 pKa = 3.39SEE133 pKa = 4.71STIDD137 pKa = 3.89IILSGSTHH145 pKa = 5.9SPEE148 pKa = 4.16TSSEE152 pKa = 4.05KK153 pKa = 10.82PEE155 pKa = 6.07DD156 pKa = 3.77IDD158 pKa = 4.64NLNCSSVFEE167 pKa = 4.36IATPEE172 pKa = 4.75PITDD176 pKa = 3.41NVEE179 pKa = 3.87DD180 pKa = 3.72HH181 pKa = 7.12TDD183 pKa = 3.32TVTYY187 pKa = 9.79TSDD190 pKa = 3.64SINTVSASSGEE201 pKa = 4.33STTDD205 pKa = 3.18EE206 pKa = 4.32TPEE209 pKa = 4.89PITDD213 pKa = 3.52KK214 pKa = 11.57EE215 pKa = 4.01EE216 pKa = 4.05DD217 pKa = 3.46HH218 pKa = 6.73TVTDD222 pKa = 3.97TVSYY226 pKa = 7.19TTVSTSSGIVTTKK239 pKa = 9.79STTDD243 pKa = 3.36DD244 pKa = 3.45ADD246 pKa = 5.14LYY248 pKa = 10.71DD249 pKa = 4.24TYY251 pKa = 11.51NDD253 pKa = 3.44NDD255 pKa = 4.39TVPSTTVGCSTTSISNYY272 pKa = 6.38KK273 pKa = 9.3TKK275 pKa = 10.95DD276 pKa = 3.25FVEE279 pKa = 4.27IFGITALIILSAVAIFCITYY299 pKa = 9.26YY300 pKa = 10.12IYY302 pKa = 11.04NKK304 pKa = 10.02RR305 pKa = 11.84SRR307 pKa = 11.84KK308 pKa = 9.53YY309 pKa = 8.36KK310 pKa = 10.04TEE312 pKa = 3.84NKK314 pKa = 9.17VV315 pKa = 2.9

Molecular weight: 34.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q77TJ2|Q77TJ2_VACCT Trans-activator protein A1 OS=Vaccinia virus (strain Tian Tan) OX=10253 PE=3 SV=1
MM1 pKa = 7.59NSTTHH6 pKa = 6.78HH7 pKa = 6.91ISSQWINVCNSRR19 pKa = 11.84TSSISYY25 pKa = 8.82VRR27 pKa = 11.84RR28 pKa = 11.84SEE30 pKa = 4.15HH31 pKa = 6.24HH32 pKa = 6.7SSTIAIEE39 pKa = 3.89CHH41 pKa = 6.59RR42 pKa = 11.84INILGIDD49 pKa = 3.68RR50 pKa = 11.84NRR52 pKa = 11.84TVGCCDD58 pKa = 3.0HH59 pKa = 6.93LHH61 pKa = 7.32RR62 pKa = 11.84INNDD66 pKa = 2.61VWCRR70 pKa = 11.84LKK72 pKa = 10.77FHH74 pKa = 6.95IVTDD78 pKa = 3.78AVYY81 pKa = 10.23RR82 pKa = 11.84SSHH85 pKa = 5.57RR86 pKa = 11.84NQRR89 pKa = 11.84RR90 pKa = 11.84SKK92 pKa = 10.8SNYY95 pKa = 7.41RR96 pKa = 11.84QNRR99 pKa = 11.84SQYY102 pKa = 11.3LFLICHH108 pKa = 6.36NLRR111 pKa = 11.84ASSMNNLHH119 pKa = 6.77

Molecular weight: 14.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

239

0

239

54410

37

1164

227.7

26.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.825 ± 0.118

2.022 ± 0.092

6.446 ± 0.135

5.323 ± 0.15

4.904 ± 0.153

3.937 ± 0.123

2.086 ± 0.083

9.531 ± 0.197

7.177 ± 0.145

9.098 ± 0.148

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.085

6.828 ± 0.128

3.325 ± 0.098

2.191 ± 0.078

4.352 ± 0.115

8.081 ± 0.138

6.01 ± 0.126

6.17 ± 0.113

0.72 ± 0.049

5.106 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski